Serine/threonine-protein phosphatase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Serine/threonine protein phosphatase 2B, putative

Species:

Leishmania major

UniProt:

Q4Q8T8_LEIMA

TriTrypDb:

LmjF.26.2530 , LMJLV39_260032300 , LMJSD75_260030700

Length:

499

Annotations

LeishMANIAdb annotations

A large collection of various protein phosphatases. Very highly expanded in kinetoplastids.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005955	calcineurin complex	3	2
GO:0008287	protein serine/threonine phosphatase complex	4	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1903293	phosphatase complex	3	2

Expansion

Sequence features

Q4Q8T8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8T8

Function

Biological processes
Term	Name	Level	Count
GO:0007165	signal transduction	2	14
GO:0009987	cellular process	1	14
GO:0019722	calcium-mediated signaling	5	14
GO:0019932	second-messenger-mediated signaling	4	14
GO:0035556	intracellular signal transduction	3	14
GO:0050789	regulation of biological process	2	14
GO:0050794	regulation of cellular process	3	14
GO:0065007	biological regulation	1	14
GO:0097720	calcineurin-mediated signaling	6	14

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0004721	phosphoprotein phosphatase activity	3	14
GO:0004722	protein serine/threonine phosphatase activity	4	14
GO:0004723	calcium-dependent protein serine/threonine phosphatase activity	5	14
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0005516	calmodulin binding	3	12
GO:0016787	hydrolase activity	2	14
GO:0016788	hydrolase activity, acting on ester bonds	3	14
GO:0016791	phosphatase activity	5	14
GO:0017018	myosin phosphatase activity	5	14
GO:0033192	calmodulin-dependent protein phosphatase activity	6	14
GO:0042578	phosphoric ester hydrolase activity	4	14
GO:0140096	catalytic activity, acting on a protein	2	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	388	392	PF00656	0.649
CLV_NRD_NRD_1	40	42	PF00675	0.495
CLV_PCSK_KEX2_1	153	155	PF00082	0.387
CLV_PCSK_KEX2_1	197	199	PF00082	0.417
CLV_PCSK_PC1ET2_1	153	155	PF00082	0.387
CLV_PCSK_PC1ET2_1	197	199	PF00082	0.417
CLV_PCSK_SKI1_1	102	106	PF00082	0.387
CLV_PCSK_SKI1_1	144	148	PF00082	0.307
CLV_PCSK_SKI1_1	154	158	PF00082	0.309
CLV_PCSK_SKI1_1	430	434	PF00082	0.763
DEG_Nend_Nbox_1	1	3	PF02207	0.678
DEG_SPOP_SBC_1	18	22	PF00917	0.482
DEG_SPOP_SBC_1	491	495	PF00917	0.490
DOC_MAPK_MEF2A_6	112	121	PF00069	0.362
DOC_PP2B_LxvP_1	238	241	PF13499	0.432
DOC_PP4_FxxP_1	208	211	PF00568	0.342
DOC_PP4_FxxP_1	296	299	PF00568	0.307
DOC_USP7_MATH_1	167	171	PF00917	0.404
DOC_USP7_MATH_1	18	22	PF00917	0.487
DOC_USP7_MATH_1	480	484	PF00917	0.508
DOC_USP7_MATH_1	491	495	PF00917	0.436
DOC_USP7_UBL2_3	283	287	PF12436	0.502
DOC_WW_Pin1_4	190	195	PF00397	0.284
DOC_WW_Pin1_4	324	329	PF00397	0.417
DOC_WW_Pin1_4	379	384	PF00397	0.675
LIG_14-3-3_CanoR_1	144	150	PF00244	0.316
LIG_14-3-3_CanoR_1	198	205	PF00244	0.422
LIG_14-3-3_CanoR_1	245	250	PF00244	0.275
LIG_14-3-3_CanoR_1	322	328	PF00244	0.363
LIG_14-3-3_CanoR_1	414	420	PF00244	0.623
LIG_Actin_WH2_2	181	199	PF00022	0.417
LIG_BRCT_BRCA1_1	21	25	PF00533	0.457
LIG_BRCT_BRCA1_1	285	289	PF00533	0.393
LIG_BRCT_BRCA1_1	342	346	PF00533	0.374
LIG_BRCT_BRCA1_1	412	416	PF00533	0.606
LIG_EH1_1	114	122	PF00400	0.417
LIG_FHA_1	339	345	PF00498	0.402
LIG_FHA_1	431	437	PF00498	0.535
LIG_FHA_1	484	490	PF00498	0.669
LIG_FHA_2	416	422	PF00498	0.545
LIG_LIR_Apic_2	219	225	PF02991	0.291
LIG_LIR_Apic_2	341	345	PF02991	0.402
LIG_LIR_Gen_1	170	180	PF02991	0.319
LIG_LIR_Gen_1	413	421	PF02991	0.633
LIG_LIR_Nem_3	147	152	PF02991	0.321
LIG_LIR_Nem_3	170	175	PF02991	0.296
LIG_LIR_Nem_3	276	282	PF02991	0.388
LIG_LIR_Nem_3	286	292	PF02991	0.356
LIG_LIR_Nem_3	413	419	PF02991	0.588
LIG_Pex14_1	342	346	PF04695	0.374
LIG_Pex14_2	336	340	PF04695	0.378
LIG_PTB_Apo_2	179	186	PF02174	0.324
LIG_SH2_CRK	165	169	PF00017	0.323
LIG_SH2_GRB2like	149	152	PF00017	0.324
LIG_SH2_GRB2like	251	254	PF00017	0.432
LIG_SH2_GRB2like	305	308	PF00017	0.347
LIG_SH2_NCK_1	251	255	PF00017	0.409
LIG_SH2_PTP2	103	106	PF00017	0.342
LIG_SH2_SRC	249	252	PF00017	0.417
LIG_SH2_STAP1	160	164	PF00017	0.312
LIG_SH2_STAP1	251	255	PF00017	0.324
LIG_SH2_STAP1	305	309	PF00017	0.363
LIG_SH2_STAT5	103	106	PF00017	0.294
LIG_SH2_STAT5	109	112	PF00017	0.284
LIG_SH2_STAT5	149	152	PF00017	0.324
LIG_SH2_STAT5	249	252	PF00017	0.362
LIG_SH2_STAT5	301	304	PF00017	0.347
LIG_SH2_STAT5	332	335	PF00017	0.382
LIG_SH3_1	6	12	PF00018	0.602
LIG_SH3_3	355	361	PF00018	0.570
LIG_SH3_3	440	446	PF00018	0.664
LIG_SH3_3	6	12	PF00018	0.492
LIG_SH3_3	68	74	PF00018	0.278
LIG_SUMO_SIM_anti_2	118	124	PF11976	0.309
LIG_SUMO_SIM_anti_2	264	270	PF11976	0.401
LIG_SUMO_SIM_anti_2	50	56	PF11976	0.359
LIG_UBA3_1	120	127	PF00899	0.346
MOD_CDK_SPxxK_3	190	197	PF00069	0.309
MOD_CK1_1	423	429	PF00069	0.689
MOD_CK1_1	483	489	PF00069	0.655
MOD_CK1_1	490	496	PF00069	0.607
MOD_CK2_1	415	421	PF00069	0.564
MOD_CK2_1	461	467	PF00069	0.520
MOD_CK2_1	473	479	PF00069	0.700
MOD_GlcNHglycan	169	172	PF01048	0.387
MOD_GlcNHglycan	21	24	PF01048	0.496
MOD_GlcNHglycan	213	216	PF01048	0.298
MOD_GlcNHglycan	26	30	PF01048	0.517
MOD_GlcNHglycan	296	299	PF01048	0.295
MOD_GlcNHglycan	482	485	PF01048	0.506
MOD_GlcNHglycan	489	492	PF01048	0.494
MOD_GSK3_1	140	147	PF00069	0.317
MOD_GSK3_1	241	248	PF00069	0.292
MOD_GSK3_1	480	487	PF00069	0.657
MOD_GSK3_1	491	498	PF00069	0.677
MOD_N-GLC_1	241	246	PF02516	0.372
MOD_N-GLC_1	324	329	PF02516	0.422
MOD_N-GLC_2	300	302	PF02516	0.321
MOD_NEK2_1	19	24	PF00069	0.490
MOD_NEK2_1	25	30	PF00069	0.507
MOD_NEK2_1	340	345	PF00069	0.370
MOD_NEK2_1	415	420	PF00069	0.571
MOD_PIKK_1	53	59	PF00454	0.401
MOD_PIKK_1	96	102	PF00454	0.354
MOD_PKA_1	197	203	PF00069	0.432
MOD_PKA_1	283	289	PF00069	0.450
MOD_PKA_2	197	203	PF00069	0.446
MOD_PKA_2	410	416	PF00069	0.618
MOD_Plk_1	423	429	PF00069	0.644
MOD_Plk_2-3	461	467	PF00069	0.343
MOD_Plk_4	130	136	PF00069	0.346
MOD_Plk_4	145	151	PF00069	0.230
MOD_Plk_4	245	251	PF00069	0.317
MOD_Plk_4	484	490	PF00069	0.482
MOD_Plk_4	50	56	PF00069	0.377
MOD_ProDKin_1	190	196	PF00069	0.284
MOD_ProDKin_1	324	330	PF00069	0.414
MOD_ProDKin_1	379	385	PF00069	0.678
MOD_SUMO_for_1	152	155	PF00179	0.342
MOD_SUMO_rev_2	193	199	PF00179	0.342
MOD_SUMO_rev_2	343	352	PF00179	0.508
TRG_DiLeu_BaEn_1	118	123	PF01217	0.342
TRG_DiLeu_BaEn_1	261	266	PF01217	0.362
TRG_DiLeu_BaEn_1	366	371	PF01217	0.671
TRG_ENDOCYTIC_2	103	106	PF00928	0.349
TRG_ENDOCYTIC_2	149	152	PF00928	0.342
TRG_ENDOCYTIC_2	165	168	PF00928	0.287
TRG_Pf-PMV_PEXEL_1	393	397	PF00026	0.616
TRG_Pf-PMV_PEXEL_1	454	458	PF00026	0.503

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7Y2	Leptomonas seymouri	75%	91%
A0A0N1ILH2	Leptomonas seymouri	38%	100%
A0A0S4JDD1	Bodo saltans	52%	100%
A0A0S4JKZ6	Bodo saltans	58%	100%
A0A0S4KLF4	Bodo saltans	25%	100%
A0A1X0NMS7	Trypanosomatidae	27%	100%
A0A1X0P6J3	Trypanosomatidae	52%	100%
A0A1X0P6Y9	Trypanosomatidae	33%	100%
A0A1X0P7H9	Trypanosomatidae	58%	100%
A0A1X0P7Q5	Trypanosomatidae	43%	100%
A0A1X0P9R6	Trypanosomatidae	30%	100%
A0A3Q8I8M6	Leishmania donovani	30%	100%
A0A3Q8ID20	Leishmania donovani	94%	100%
A0A3R7M399	Trypanosoma rangeli	30%	100%
A0A3R7MBA3	Trypanosoma rangeli	55%	100%
A0A3S5H6K2	Leishmania donovani	25%	100%
A0A3S5H6T9	Leishmania donovani	36%	100%
A0A3S5H7Q6	Leishmania donovani	36%	100%
A0A3S7XAY3	Leishmania donovani	39%	100%
A0A422N1U7	Trypanosoma rangeli	27%	83%
A0A422N9D5	Trypanosoma rangeli	45%	100%
A4H6S0	Leishmania braziliensis	25%	100%
A4H7Z3	Leishmania braziliensis	35%	100%
A4HF98	Leishmania braziliensis	77%	91%
A4HJQ1	Leishmania braziliensis	36%	100%
A4HP65	Leishmania braziliensis	37%	100%
A4HV49	Leishmania infantum	25%	100%
A4HVT0	Leishmania infantum	31%	100%
A4HWC1	Leishmania infantum	36%	100%
A4I2J0	Leishmania infantum	94%	100%
A4I768	Leishmania infantum	36%	100%
A4IDH0	Leishmania infantum	39%	100%
C9ZM48	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
C9ZQD6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
C9ZX13	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
D0A358	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
D0A366	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	78%
E9ANT1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9APH5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AQ21	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9ASX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AYN4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	99%
E9B262	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
O42773	Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)	46%	78%
P14747	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	83%
P16298	Homo sapiens	48%	95%
P20651	Rattus norvegicus	46%	95%
P23287	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	90%
P48452	Bos taurus	48%	96%
P48453	Mus musculus	46%	95%
P48454	Homo sapiens	47%	97%
P48455	Mus musculus	45%	97%
P48456	Drosophila melanogaster	48%	80%
P48457	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	46%	91%
P63328	Mus musculus	48%	96%
P63329	Rattus norvegicus	48%	96%
Q05681	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	48%	89%
Q08209	Homo sapiens	48%	96%
Q0G819	Caenorhabditis elegans	46%	92%
Q12705	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	47%	90%
Q27889	Drosophila melanogaster	48%	88%
Q4Q1M5	Leishmania major	37%	100%
Q4Q5Z8	Leishmania major	37%	100%
Q4QFG0	Leishmania major	35%	100%
Q4QG03	Leishmania major	31%	100%
Q4QGT8	Leishmania major	26%	100%
Q4WUR1	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	46%	93%
Q7YSW8	Dictyostelium discoideum	45%	80%
Q9FN02	Arabidopsis thaliana	34%	100%
Q9VXF1	Drosophila melanogaster	49%	85%
V5B3V2	Trypanosoma cruzi	52%	100%
V5BBR3	Trypanosoma cruzi	47%	100%
V5BBS3	Trypanosoma cruzi	27%	83%