N2227 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4Q8R9_LEIMA

TriTrypDb:

LmjF.28.0010 , LMJLV39_280005100 , LMJSD75_280005000

Length:

550

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4Q8R9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8R9

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	3
GO:0006796	phosphate-containing compound metabolic process	4	3
GO:0008152	metabolic process	1	3
GO:0009987	cellular process	1	3
GO:0016310	phosphorylation	5	3
GO:0044237	cellular metabolic process	2	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	113	117	PF00656	0.466
CLV_C14_Caspase3-7	288	292	PF00656	0.425
CLV_NRD_NRD_1	105	107	PF00675	0.323
CLV_NRD_NRD_1	141	143	PF00675	0.486
CLV_NRD_NRD_1	2	4	PF00675	0.669
CLV_NRD_NRD_1	261	263	PF00675	0.366
CLV_NRD_NRD_1	293	295	PF00675	0.463
CLV_NRD_NRD_1	376	378	PF00675	0.339
CLV_NRD_NRD_1	410	412	PF00675	0.520
CLV_NRD_NRD_1	512	514	PF00675	0.586
CLV_NRD_NRD_1	545	547	PF00675	0.539
CLV_PCSK_KEX2_1	105	107	PF00082	0.323
CLV_PCSK_KEX2_1	141	143	PF00082	0.342
CLV_PCSK_KEX2_1	2	4	PF00082	0.509
CLV_PCSK_KEX2_1	376	378	PF00082	0.339
CLV_PCSK_KEX2_1	410	412	PF00082	0.404
CLV_PCSK_KEX2_1	51	53	PF00082	0.475
CLV_PCSK_KEX2_1	544	546	PF00082	0.604
CLV_PCSK_PC1ET2_1	410	412	PF00082	0.404
CLV_PCSK_PC1ET2_1	51	53	PF00082	0.475
CLV_PCSK_PC7_1	101	107	PF00082	0.314
CLV_PCSK_PC7_1	540	546	PF00082	0.508
CLV_PCSK_SKI1_1	15	19	PF00082	0.381
CLV_PCSK_SKI1_1	181	185	PF00082	0.490
CLV_PCSK_SKI1_1	2	6	PF00082	0.606
CLV_PCSK_SKI1_1	457	461	PF00082	0.493
CLV_PCSK_SKI1_1	540	544	PF00082	0.453
CLV_PCSK_SKI1_1	546	550	PF00082	0.487
CLV_PCSK_SKI1_1	96	100	PF00082	0.481
DEG_APCC_DBOX_1	2	10	PF00400	0.596
DEG_APCC_DBOX_1	28	36	PF00400	0.376
DEG_APCC_DBOX_1	413	421	PF00400	0.313
DEG_Nend_UBRbox_1	1	4	PF02207	0.536
DEG_SCF_FBW7_1	339	344	PF00400	0.277
DOC_CKS1_1	384	389	PF01111	0.461
DOC_CYCLIN_RxL_1	10	23	PF00134	0.493
DOC_CYCLIN_RxL_1	222	233	PF00134	0.334
DOC_CYCLIN_yCln2_LP_2	179	185	PF00134	0.516
DOC_CYCLIN_yCln2_LP_2	190	196	PF00134	0.433
DOC_MAPK_gen_1	267	276	PF00069	0.386
DOC_MAPK_gen_1	294	303	PF00069	0.337
DOC_MAPK_gen_1	373	381	PF00069	0.345
DOC_MAPK_gen_1	410	419	PF00069	0.347
DOC_MAPK_gen_1	445	453	PF00069	0.610
DOC_MAPK_gen_1	457	465	PF00069	0.469
DOC_MAPK_MEF2A_6	201	210	PF00069	0.413
DOC_PP2B_LxvP_1	190	193	PF13499	0.463
DOC_PP4_FxxP_1	217	220	PF00568	0.335
DOC_PP4_FxxP_1	398	401	PF00568	0.470
DOC_PP4_FxxP_1	55	58	PF00568	0.290
DOC_SPAK_OSR1_1	377	381	PF12202	0.468
DOC_USP7_MATH_1	81	85	PF00917	0.447
DOC_USP7_UBL2_3	445	449	PF12436	0.540
DOC_WW_Pin1_4	265	270	PF00397	0.569
DOC_WW_Pin1_4	337	342	PF00397	0.373
DOC_WW_Pin1_4	383	388	PF00397	0.419
DOC_WW_Pin1_4	535	540	PF00397	0.420
LIG_14-3-3_CanoR_1	15	20	PF00244	0.483
LIG_14-3-3_CanoR_1	169	175	PF00244	0.419
LIG_14-3-3_CanoR_1	411	420	PF00244	0.342
LIG_14-3-3_CanoR_1	497	501	PF00244	0.569
LIG_14-3-3_CterR_2	546	550	PF00244	0.557
LIG_deltaCOP1_diTrp_1	107	114	PF00928	0.351
LIG_EVH1_2	198	202	PF00568	0.350
LIG_FHA_1	155	161	PF00498	0.452
LIG_FHA_1	338	344	PF00498	0.284
LIG_FHA_1	394	400	PF00498	0.554
LIG_FHA_1	421	427	PF00498	0.425
LIG_FHA_1	88	94	PF00498	0.487
LIG_FHA_2	108	114	PF00498	0.460
LIG_GBD_Chelix_1	492	500	PF00786	0.442
LIG_LIR_Apic_2	363	367	PF02991	0.580
LIG_LIR_Apic_2	382	387	PF02991	0.260
LIG_LIR_Apic_2	396	401	PF02991	0.400
LIG_LIR_Gen_1	162	171	PF02991	0.314
LIG_LIR_Gen_1	333	343	PF02991	0.484
LIG_LIR_Gen_1	44	55	PF02991	0.335
LIG_LIR_Nem_3	107	111	PF02991	0.314
LIG_LIR_Nem_3	148	153	PF02991	0.346
LIG_LIR_Nem_3	162	167	PF02991	0.298
LIG_LIR_Nem_3	170	174	PF02991	0.320
LIG_LIR_Nem_3	280	285	PF02991	0.558
LIG_LIR_Nem_3	333	339	PF02991	0.471
LIG_LIR_Nem_3	44	50	PF02991	0.335
LIG_LIR_Nem_3	469	473	PF02991	0.455
LIG_LIR_Nem_3	501	507	PF02991	0.575
LIG_LIR_Nem_3	78	83	PF02991	0.367
LIG_LYPXL_yS_3	504	507	PF13949	0.562
LIG_LYPXL_yS_3	80	83	PF13949	0.377
LIG_Pex14_1	213	217	PF04695	0.329
LIG_PTB_Apo_2	124	131	PF02174	0.334
LIG_PTB_Apo_2	270	277	PF02174	0.426
LIG_REV1ctd_RIR_1	333	340	PF16727	0.473
LIG_SH2_CRK	194	198	PF00017	0.489
LIG_SH2_CRK	298	302	PF00017	0.334
LIG_SH2_CRK	384	388	PF00017	0.352
LIG_SH2_CRK	47	51	PF00017	0.459
LIG_SH2_NCK_1	384	388	PF00017	0.481
LIG_SH2_PTP2	164	167	PF00017	0.436
LIG_SH2_PTP2	34	37	PF00017	0.297
LIG_SH2_SRC	34	37	PF00017	0.444
LIG_SH2_STAP1	161	165	PF00017	0.278
LIG_SH2_STAP1	298	302	PF00017	0.334
LIG_SH2_STAP1	370	374	PF00017	0.386
LIG_SH2_STAT5	129	132	PF00017	0.378
LIG_SH2_STAT5	146	149	PF00017	0.384
LIG_SH2_STAT5	164	167	PF00017	0.272
LIG_SH2_STAT5	34	37	PF00017	0.318
LIG_SH2_STAT5	534	537	PF00017	0.550
LIG_SH3_3	179	185	PF00018	0.354
LIG_SH3_3	359	365	PF00018	0.383
LIG_SH3_3	499	505	PF00018	0.437
LIG_SUMO_SIM_anti_2	205	211	PF11976	0.451
LIG_SUMO_SIM_anti_2	40	47	PF11976	0.266
LIG_TRAF2_1	305	308	PF00917	0.476
LIG_TYR_ITIM	192	197	PF00017	0.363
LIG_TYR_ITIM	32	37	PF00017	0.325
LIG_TYR_ITIM	45	50	PF00017	0.301
LIG_UBA3_1	207	212	PF00899	0.455
LIG_WRC_WIRS_1	168	173	PF05994	0.318
MOD_CDK_SPK_2	265	270	PF00069	0.446
MOD_CDK_SPK_2	535	540	PF00069	0.420
MOD_CDK_SPxK_1	383	389	PF00069	0.458
MOD_CK1_1	154	160	PF00069	0.403
MOD_CK1_1	170	176	PF00069	0.465
MOD_CK1_1	200	206	PF00069	0.412
MOD_CK1_1	272	278	PF00069	0.574
MOD_CK2_1	169	175	PF00069	0.389
MOD_CK2_1	535	541	PF00069	0.479
MOD_GlcNHglycan	232	235	PF01048	0.341
MOD_GlcNHglycan	310	313	PF01048	0.460
MOD_GlcNHglycan	390	393	PF01048	0.563
MOD_GlcNHglycan	83	86	PF01048	0.449
MOD_GSK3_1	265	272	PF00069	0.475
MOD_GSK3_1	337	344	PF00069	0.414
MOD_GSK3_1	492	499	PF00069	0.596
MOD_N-GLC_1	119	124	PF02516	0.341
MOD_N-GLC_1	272	277	PF02516	0.426
MOD_NEK2_1	119	124	PF00069	0.337
MOD_NEK2_1	167	172	PF00069	0.327
MOD_NEK2_1	5	10	PF00069	0.609
MOD_NEK2_1	526	531	PF00069	0.474
MOD_PIKK_1	133	139	PF00454	0.329
MOD_PIKK_1	200	206	PF00454	0.502
MOD_PK_1	422	428	PF00069	0.458
MOD_PKA_2	388	394	PF00069	0.541
MOD_PKA_2	496	502	PF00069	0.576
MOD_Plk_1	119	125	PF00069	0.333
MOD_Plk_1	154	160	PF00069	0.321
MOD_Plk_1	21	27	PF00069	0.455
MOD_Plk_1	272	278	PF00069	0.428
MOD_Plk_2-3	107	113	PF00069	0.465
MOD_Plk_4	175	181	PF00069	0.366
MOD_ProDKin_1	265	271	PF00069	0.561
MOD_ProDKin_1	337	343	PF00069	0.366
MOD_ProDKin_1	383	389	PF00069	0.428
MOD_ProDKin_1	535	541	PF00069	0.419
MOD_SUMO_rev_2	284	292	PF00179	0.407
MOD_SUMO_rev_2	369	374	PF00179	0.358
MOD_SUMO_rev_2	469	475	PF00179	0.622
TRG_DiLeu_BaEn_1	163	168	PF01217	0.271
TRG_DiLeu_BaEn_1	175	180	PF01217	0.266
TRG_DiLeu_BaEn_1	205	210	PF01217	0.449
TRG_DiLeu_BaEn_1	41	46	PF01217	0.307
TRG_DiLeu_BaLyEn_6	473	478	PF01217	0.493
TRG_ENDOCYTIC_2	150	153	PF00928	0.339
TRG_ENDOCYTIC_2	164	167	PF00928	0.267
TRG_ENDOCYTIC_2	194	197	PF00928	0.490
TRG_ENDOCYTIC_2	298	301	PF00928	0.337
TRG_ENDOCYTIC_2	34	37	PF00928	0.354
TRG_ENDOCYTIC_2	47	50	PF00928	0.344
TRG_ENDOCYTIC_2	504	507	PF00928	0.562
TRG_ENDOCYTIC_2	80	83	PF00928	0.377
TRG_ER_diArg_1	1	3	PF00400	0.580
TRG_ER_diArg_1	104	106	PF00400	0.308
TRG_ER_diArg_1	140	142	PF00400	0.482
TRG_ER_diArg_1	254	257	PF00400	0.493
TRG_ER_diArg_1	376	378	PF00400	0.339
TRG_ER_diArg_1	543	546	PF00400	0.524
TRG_NLS_MonoExtC_3	293	299	PF00514	0.515
TRG_NLS_MonoExtC_3	409	414	PF00514	0.468
TRG_NLS_MonoExtN_4	293	298	PF00514	0.376
TRG_NLS_MonoExtN_4	407	414	PF00514	0.463
TRG_Pf-PMV_PEXEL_1	411	416	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	479	483	PF00026	0.528

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I7B7	Leptomonas seymouri	75%	100%
A0A0S4IYT6	Bodo saltans	48%	90%
A0A1X0NV91	Trypanosomatidae	55%	99%
A0A3Q8IEE9	Leishmania donovani	96%	100%
A0A422NKU0	Trypanosoma rangeli	55%	99%
A4I374	Leishmania infantum	96%	100%
D0A7Q0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	99%
E9AIS9	Leishmania braziliensis	85%	100%
E9AZG2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5AS03	Trypanosoma cruzi	53%	99%