Proteasome subunit beta

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

proteasome beta 3 subunit, putative

Species:

Leishmania major

UniProt:

Q4Q8Q9_LEIMA

TriTrypDb:

LmjF.28.0110 , LMJLV39_280006200 , LMJSD75_280006100

Length:

205

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	11
GO:0005839	proteasome core complex	3	2
GO:0019774	proteasome core complex, beta-subunit complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

Q4Q8Q9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8Q9

PDB structure(s): 6qm7_J , 6qm7_X , 6qm8_J , 6qm8_X , 6tcz_j , 6td5_j

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006511	ubiquitin-dependent protein catabolic process	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010498	proteasomal protein catabolic process	5	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	12
GO:0030163	protein catabolic process	4	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043632	modification-dependent macromolecule catabolic process	5	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0051603	proteolysis involved in protein catabolic process	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901565	organonitrogen compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0016787	hydrolase activity	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	202	204	PF00675	0.493
CLV_PCSK_KEX2_1	26	28	PF00082	0.427
CLV_PCSK_PC1ET2_1	26	28	PF00082	0.427
CLV_PCSK_SKI1_1	198	202	PF00082	0.486
CLV_PCSK_SKI1_1	80	84	PF00082	0.377
DEG_Kelch_Keap1_1	113	118	PF01344	0.544
DOC_PP1_RVXF_1	64	70	PF00149	0.534
DOC_PP4_FxxP_1	104	107	PF00568	0.522
DOC_USP7_MATH_1	142	146	PF00917	0.608
DOC_WW_Pin1_4	156	161	PF00397	0.604
LIG_BIR_II_1	1	5	PF00653	0.458
LIG_FHA_1	114	120	PF00498	0.526
LIG_FHA_1	122	128	PF00498	0.517
LIG_FHA_1	194	200	PF00498	0.456
LIG_FHA_1	90	96	PF00498	0.576
LIG_FHA_2	139	145	PF00498	0.522
LIG_LIR_Gen_1	18	28	PF02991	0.534
LIG_LIR_Nem_3	18	23	PF02991	0.534
LIG_LIR_Nem_3	181	186	PF02991	0.527
LIG_Pex14_2	100	104	PF04695	0.522
LIG_SH2_CRK	183	187	PF00017	0.534
LIG_SH2_STAP1	96	100	PF00017	0.522
LIG_SH2_STAT5	120	123	PF00017	0.522
LIG_SH2_STAT5	136	139	PF00017	0.522
LIG_SH3_3	127	133	PF00018	0.514
LIG_TRAF2_1	159	162	PF00917	0.608
MOD_CK1_1	2	8	PF00069	0.446
MOD_CK2_1	112	118	PF00069	0.600
MOD_CK2_1	138	144	PF00069	0.522
MOD_CK2_1	156	162	PF00069	0.608
MOD_Cter_Amidation	201	204	PF01082	0.500
MOD_GlcNHglycan	172	175	PF01048	0.322
MOD_GlcNHglycan	180	183	PF01048	0.297
MOD_GlcNHglycan	6	9	PF01048	0.391
MOD_GSK3_1	108	115	PF00069	0.620
MOD_GSK3_1	138	145	PF00069	0.523
MOD_GSK3_1	51	58	PF00069	0.515
MOD_N-GLC_1	45	50	PF02516	0.363
MOD_NEK2_1	4	9	PF00069	0.425
MOD_NEK2_1	90	95	PF00069	0.520
MOD_NEK2_2	142	147	PF00069	0.534
MOD_PKA_2	176	182	PF00069	0.534
MOD_Plk_1	45	51	PF00069	0.563
MOD_Plk_4	45	51	PF00069	0.541
MOD_Plk_4	7	13	PF00069	0.598
MOD_Plk_4	90	96	PF00069	0.521
MOD_ProDKin_1	156	162	PF00069	0.604
TRG_DiLeu_BaLyEn_6	195	200	PF01217	0.471
TRG_ENDOCYTIC_2	183	186	PF00928	0.530
TRG_Pf-PMV_PEXEL_1	26	30	PF00026	0.427

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1ILF0	Leptomonas seymouri	24%	83%
A0A0N1PCD1	Leptomonas seymouri	92%	100%
A0A0S4IV44	Bodo saltans	75%	100%
A0A0S4J7A1	Bodo saltans	24%	83%
A0A1X0NK57	Trypanosomatidae	25%	83%
A0A1X0NVR6	Trypanosomatidae	85%	100%
A0A3R7KX47	Trypanosoma rangeli	27%	83%
A0A3S7WPD8	Leishmania donovani	25%	83%
A0A3S7X127	Leishmania donovani	99%	100%
A0A422MWT4	Trypanosoma rangeli	85%	100%
A0B5B1	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	26%	99%
A0RU86	Cenarchaeum symbiosum (strain A)	27%	100%
A1RSJ8	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	26%	100%
A1RTI7	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	24%	100%
A1RWY6	Thermofilum pendens (strain DSM 2475 / Hrk 5)	23%	100%
A1RX71	Thermofilum pendens (strain DSM 2475 / Hrk 5)	27%	100%
A2BN24	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	24%	100%
A2BN27	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	25%	97%
A2SS78	Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)	29%	97%
A3CUS9	Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)	26%	96%
A3DN21	Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)	26%	96%
A3DN27	Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)	27%	99%
A3MS44	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	22%	100%
A3MXQ6	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	28%	100%
A4FYA5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	23%	94%
A4HG53	Leishmania braziliensis	94%	100%
A4HSQ5	Leishmania infantum	25%	83%
A4I384	Leishmania infantum	99%	100%
A4WH05	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	25%	100%
A4WMZ0	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	23%	100%
A4YIE0	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	24%	94%
A4YJ04	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	26%	100%
A5UM14	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	26%	100%
A6USJ3	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	23%	94%
A6UT20	Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)	21%	94%
A6VK02	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	23%	94%
A7I841	Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)	27%	96%
A7KE01	Salmo salar	23%	94%
A7KII6	Salmo salar	23%	94%
A8AA46	Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125)	25%	100%
A8AB58	Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125)	26%	97%
A8M8R5	Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167)	23%	100%
A8MBW0	Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167)	23%	100%
A9A4A1	Nitrosopumilus maritimus (strain SCM1)	26%	100%
A9A788	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	23%	94%
B0R4C8	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	23%	84%
B1L6S7	Korarchaeum cryptofilum (strain OPF8)	25%	100%
B1L6X8	Korarchaeum cryptofilum (strain OPF8)	30%	100%
B1YA36	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	25%	100%
B1YDJ0	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	28%	100%
B5IEE5	Aciduliprofundum boonei (strain DSM 19572 / T469)	25%	98%
B6YSW2	Thermococcus onnurineus (strain NA1)	23%	100%
B6YXV3	Thermococcus onnurineus (strain NA1)	23%	100%
B8D673	Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n)	28%	100%
B8D683	Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n)	25%	100%
B8GG66	Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)	25%	97%
B8H8L6	Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6)	28%	75%
B9LTS6	Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)	23%	84%
C3MRE5	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	24%	100%
C3MY41	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	24%	100%
C3MZI0	Sulfolobus islandicus (strain M.16.27)	24%	100%
C3N7K2	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	24%	100%
C3NFX2	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	24%	100%
C4KIR0	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	24%	100%
C5A2D5	Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)	27%	100%
C5A7L1	Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)	24%	100%
C6A2V9	Thermococcus sibiricus (strain DSM 12597 / MM 739)	22%	99%
C7NQ99	Halorhabdus utahensis (strain DSM 12940 / JCM 11049 / AX-2)	26%	88%
C7NVQ4	Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541)	29%	86%
C7P6N4	Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86)	25%	92%
C9REN7	Methanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7)	25%	92%
C9ZTA1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	79%
D0A7R1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	86%	100%
D0KRX1	Saccharolobus solfataricus (strain 98/2)	24%	100%
D1Z199	Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE)	23%	100%
D2PDG6	Sulfolobus islandicus (strain L.D.8.5 / Lassen #2)	24%	100%
D2RGT4	Archaeoglobus profundus (strain DSM 5631 / JCM 9629 / NBRC 100127 / Av18)	28%	95%
D2RQX3	Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k)	27%	84%
D2RUI0	Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k)	27%	86%
D3E0A3	Methanobrevibacter ruminantium (strain ATCC 35063 / DSM 1093 / JCM 13430 / OCM 146 / M1)	25%	99%
D3RX66	Ferroglobus placidus (strain DSM 10642 / AEDII12DO)	28%	98%
D3S8M7	Methanocaldococcus sp. (strain FS406-22)	26%	92%
D3SSQ6	Natrialba magadii (strain ATCC 43099 / DSM 3394 / CCM 3739 / CIP 104546 / IAM 13178 / JCM 8861 / NBRC 102185 / NCIMB 2190 / MS3)	26%	84%
D4GYZ1	Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)	24%	84%
D5EAS6	Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP)	25%	98%
E9AKP2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	83%
E9AZH2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O09061	Mus musculus	27%	85%
O27270	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	23%	98%
O35521	Mus terricolor	23%	94%
O35522	Mus musculus bactrianus	23%	94%
O35524	Mus spicilegus	23%	94%
O43063	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	91%
O50110	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	20%	99%
O64464	Oryza sativa subsp. japonica	22%	93%
O65084	Picea mariana	45%	100%
O73817	Oncorhynchus mykiss	49%	100%
O81153	Arabidopsis thaliana	45%	100%
P18421	Rattus norvegicus	27%	85%
P20618	Homo sapiens	27%	85%
P23724	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	85%
P25451	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	43%	100%
P28061	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	22%	97%
P28065	Homo sapiens	23%	94%
P28076	Mus musculus	23%	94%
P28077	Rattus norvegicus	24%	94%
P33672	Bos taurus	47%	100%
P34286	Caenorhabditis elegans	22%	79%
P38624	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	95%
P40112	Rattus norvegicus	47%	100%
P40304	Drosophila melanogaster	28%	87%
P42742	Arabidopsis thaliana	24%	92%
P49720	Homo sapiens	47%	100%
Q0W2D6	Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)	28%	99%
Q12YV7	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	26%	98%
Q18GX3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	23%	84%
Q23237	Caenorhabditis elegans	46%	100%
Q2NI68	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	28%	100%
Q2TBX6	Bos taurus	27%	85%
Q3IPX1	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	29%	86%
Q3SZC2	Bos taurus	24%	94%
Q46G14	Methanosarcina barkeri (strain Fusaro / DSM 804)	25%	97%
Q4JAA8	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	25%	100%
Q4JAY3	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	26%	100%
Q4QGU1	Leishmania major	25%	72%
Q4QJ65	Leishmania major	25%	83%
Q55D66	Dictyostelium discoideum	41%	100%
Q58634	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	26%	92%
Q5B7I7	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	23%	95%
Q5JHL8	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	24%	100%
Q5V1D5	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	25%	89%
Q5V4S6	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	27%	85%
Q5W416	Canis lupus familiaris	24%	74%
Q6L181	Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)	21%	98%
Q6LZD4	Methanococcus maripaludis (strain S2 / LL)	23%	94%
Q74MP5	Nanoarchaeum equitans (strain Kin4-M)	23%	100%
Q86A21	Dictyostelium discoideum	22%	87%
Q8PZ04	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	25%	98%
Q8SR11	Encephalitozoon cuniculi (strain GB-M1)	23%	100%
Q8TJB5	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	24%	98%
Q8TW10	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	25%	98%
Q8U125	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	22%	100%
Q8U4C9	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	24%	100%
Q8UW64	Oryzias latipes	24%	94%
Q8ZST5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	27%	100%
Q8ZYF2	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	23%	100%
Q975D1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	26%	99%
Q975U8	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	23%	100%
Q97AZ7	Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)	22%	97%
Q980L4	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	24%	100%
Q9DD33	Salmo salar	23%	94%
Q9HR36	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	23%	84%
Q9IB83	Carassius auratus	29%	86%
Q9IB84	Carassius auratus	29%	86%
Q9LST7	Oryza sativa subsp. japonica	45%	100%
Q9N9W8	Giardia intestinalis	34%	99%
Q9NDA1	Trypanosoma brucei brucei	86%	100%
Q9P992	Methanosarcina thermophila	25%	98%
Q9P996	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	28%	96%
Q9PT26	Oncorhynchus mykiss	23%	94%
Q9R1P1	Mus musculus	47%	100%
Q9U794	Trypanosoma brucei brucei	26%	79%
Q9UQY2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	91%
Q9V0N9	Pyrococcus abyssi (strain GE5 / Orsay)	22%	99%
Q9XI05	Arabidopsis thaliana	46%	100%
Q9XYN7	Drosophila melanogaster	49%	100%
Q9Y7T8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	45%	100%
Q9YER0	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	24%	94%
Q9YES4	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	29%	100%
V5BGL9	Trypanosoma cruzi	83%	100%