Putative ubiquitin activating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin activating enzyme, putative

Species:

Leishmania major

UniProt:

Q4Q8N4_LEIMA

TriTrypDb:

LmjF.28.0360 , LMJLV39_280008800 , LMJSD75_280008900

Length:

287

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031510	SUMO activating enzyme complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

Q4Q8N4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8N4

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0016925	protein sumoylation	7	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018205	peptidyl-lysine modification	6	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	12
GO:0016874	ligase activity	2	12
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	12
GO:0019948	SUMO activating enzyme activity	3	2
GO:0140096	catalytic activity, acting on a protein	2	12
GO:0140657	ATP-dependent activity	1	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	102	106	PF00656	0.482
CLV_C14_Caspase3-7	62	66	PF00656	0.376
CLV_NRD_NRD_1	154	156	PF00675	0.448
CLV_PCSK_KEX2_1	154	156	PF00082	0.515
CLV_PCSK_SKI1_1	184	188	PF00082	0.396
DEG_Nend_UBRbox_1	1	4	PF02207	0.488
DOC_MAPK_gen_1	192	200	PF00069	0.362
DOC_MAPK_MEF2A_6	184	191	PF00069	0.427
DOC_MAPK_MEF2A_6	192	200	PF00069	0.491
DOC_MAPK_MEF2A_6	90	97	PF00069	0.435
DOC_MAPK_NFAT4_5	184	192	PF00069	0.435
DOC_USP7_MATH_1	115	119	PF00917	0.411
DOC_USP7_MATH_1	157	161	PF00917	0.555
DOC_USP7_MATH_1	170	174	PF00917	0.511
DOC_USP7_MATH_1	93	97	PF00917	0.429
LIG_14-3-3_CanoR_1	111	115	PF00244	0.486
LIG_BIR_III_2	122	126	PF00653	0.577
LIG_BRCT_BRCA1_1	196	200	PF00533	0.565
LIG_FHA_1	66	72	PF00498	0.415
LIG_FHA_2	100	106	PF00498	0.434
LIG_FHA_2	117	123	PF00498	0.531
LIG_FHA_2	218	224	PF00498	0.429
LIG_FHA_2	60	66	PF00498	0.415
LIG_GBD_Chelix_1	148	156	PF00786	0.480
LIG_LIR_Gen_1	212	221	PF02991	0.490
LIG_LIR_Gen_1	269	274	PF02991	0.449
LIG_LIR_Gen_1	96	104	PF02991	0.408
LIG_LIR_Nem_3	163	168	PF02991	0.488
LIG_LIR_Nem_3	212	216	PF02991	0.480
LIG_LIR_Nem_3	96	101	PF02991	0.408
LIG_PDZ_Class_2	282	287	PF00595	0.494
LIG_SH2_CRK	153	157	PF00017	0.525
LIG_SH2_CRK	98	102	PF00017	0.408
LIG_SH2_NCK_1	208	212	PF00017	0.499
LIG_SH2_NCK_1	98	102	PF00017	0.408
LIG_SH2_SRC	231	234	PF00017	0.392
LIG_SH2_STAP1	9	13	PF00017	0.298
LIG_SH2_STAT3	70	73	PF00017	0.408
LIG_SH2_STAT3	9	12	PF00017	0.298
LIG_SH2_STAT5	70	73	PF00017	0.408
LIG_SH3_3	166	172	PF00018	0.497
LIG_TRAF2_1	118	121	PF00917	0.382
MOD_CK1_1	107	113	PF00069	0.451
MOD_CK2_1	115	121	PF00069	0.435
MOD_CK2_1	157	163	PF00069	0.488
MOD_GlcNHglycan	107	110	PF01048	0.535
MOD_GlcNHglycan	18	21	PF01048	0.434
MOD_GlcNHglycan	236	239	PF01048	0.556
MOD_GSK3_1	139	146	PF00069	0.433
MOD_GSK3_1	190	197	PF00069	0.535
MOD_NEK2_1	104	109	PF00069	0.546
MOD_NEK2_1	139	144	PF00069	0.425
MOD_NEK2_1	24	29	PF00069	0.332
MOD_NEK2_2	93	98	PF00069	0.477
MOD_PKA_2	110	116	PF00069	0.493
MOD_Plk_4	143	149	PF00069	0.424
MOD_Plk_4	170	176	PF00069	0.477
MOD_Plk_4	209	215	PF00069	0.472
MOD_Plk_4	93	99	PF00069	0.491
TRG_DiLeu_BaEn_1	280	285	PF01217	0.521
TRG_ENDOCYTIC_2	153	156	PF00928	0.507
TRG_ENDOCYTIC_2	208	211	PF00928	0.462
TRG_ENDOCYTIC_2	270	273	PF00928	0.404
TRG_ENDOCYTIC_2	98	101	PF00928	0.313
TRG_ER_diArg_1	153	155	PF00400	0.545
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2G3	Leptomonas seymouri	55%	100%
A0A0S4IP88	Bodo saltans	26%	82%
A0A1X0NVF2	Trypanosomatidae	30%	97%
A0A3S7X162	Leishmania donovani	95%	100%
A0A422N6R9	Trypanosoma rangeli	32%	98%
A4HG77	Leishmania braziliensis	86%	100%
A4HKL5	Leishmania braziliensis	85%	100%
A4I3A8	Leishmania infantum	95%	100%
D0A7U0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	97%
E9AZJ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5DAI2	Trypanosoma cruzi	36%	98%