EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

SNARE associated Golgi protein, putative

Species:

Leishmania major

UniProt:

Q4Q8J8_LEIMA

TriTrypDb:

LmjF.28.0710 , LMJLV39_280012800 , LMJSD75_280012800

Length:

585

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	2
GO:0012505	endomembrane system	2	2
GO:0016020	membrane	2	10
GO:0031967	organelle envelope	3	2
GO:0031975	envelope	2	2
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4Q8J8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8J8

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	10
GO:0005509	calcium ion binding	5	10
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	141	145	PF00656	0.299
CLV_NRD_NRD_1	19	21	PF00675	0.359
CLV_NRD_NRD_1	309	311	PF00675	0.331
CLV_NRD_NRD_1	480	482	PF00675	0.351
CLV_NRD_NRD_1	569	571	PF00675	0.637
CLV_PCSK_KEX2_1	19	21	PF00082	0.359
CLV_PCSK_KEX2_1	309	311	PF00082	0.292
CLV_PCSK_KEX2_1	414	416	PF00082	0.398
CLV_PCSK_KEX2_1	479	481	PF00082	0.333
CLV_PCSK_KEX2_1	569	571	PF00082	0.637
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.398
CLV_PCSK_PC7_1	565	571	PF00082	0.571
CLV_PCSK_SKI1_1	414	418	PF00082	0.387
CLV_PCSK_SKI1_1	494	498	PF00082	0.353
CLV_PCSK_SKI1_1	520	524	PF00082	0.404
CLV_PCSK_SKI1_1	53	57	PF00082	0.350
CLV_PCSK_SKI1_1	569	573	PF00082	0.557
DEG_APCC_DBOX_1	18	26	PF00400	0.580
DEG_APCC_DBOX_1	29	37	PF00400	0.531
DOC_CYCLIN_RxL_1	50	58	PF00134	0.451
DOC_MAPK_FxFP_2	191	194	PF00069	0.335
DOC_MAPK_gen_1	223	230	PF00069	0.548
DOC_MAPK_gen_1	27	36	PF00069	0.542
DOC_MAPK_gen_1	569	576	PF00069	0.423
DOC_MAPK_MEF2A_6	223	230	PF00069	0.612
DOC_MAPK_MEF2A_6	352	359	PF00069	0.289
DOC_MAPK_MEF2A_6	540	548	PF00069	0.430
DOC_PP4_FxxP_1	191	194	PF00568	0.335
DOC_PP4_FxxP_1	219	222	PF00568	0.549
DOC_PP4_FxxP_1	337	340	PF00568	0.236
DOC_USP7_MATH_1	408	412	PF00917	0.574
DOC_USP7_MATH_1	504	508	PF00917	0.611
DOC_USP7_MATH_1	97	101	PF00917	0.412
DOC_USP7_UBL2_3	352	356	PF12436	0.271
DOC_WW_Pin1_4	481	486	PF00397	0.659
DOC_WW_Pin1_4	74	79	PF00397	0.367
LIG_14-3-3_CanoR_1	126	132	PF00244	0.318
LIG_14-3-3_CanoR_1	19	23	PF00244	0.598
LIG_14-3-3_CanoR_1	27	36	PF00244	0.575
LIG_14-3-3_CanoR_1	379	389	PF00244	0.624
LIG_14-3-3_CanoR_1	505	513	PF00244	0.605
LIG_14-3-3_CanoR_1	540	545	PF00244	0.338
LIG_BIR_II_1	1	5	PF00653	0.642
LIG_BRCT_BRCA1_1	341	345	PF00533	0.282
LIG_CaM_NSCaTE_8	314	321	PF13499	0.314
LIG_deltaCOP1_diTrp_1	161	170	PF00928	0.384
LIG_FHA_1	102	108	PF00498	0.333
LIG_FHA_1	29	35	PF00498	0.544
LIG_FHA_1	545	551	PF00498	0.398
LIG_FHA_1	56	62	PF00498	0.335
LIG_FHA_2	139	145	PF00498	0.390
LIG_FHA_2	468	474	PF00498	0.637
LIG_FHA_2	487	493	PF00498	0.463
LIG_FHA_2	93	99	PF00498	0.347
LIG_GBD_Chelix_1	164	172	PF00786	0.389
LIG_LIR_Apic_2	216	222	PF02991	0.560
LIG_LIR_Apic_2	71	76	PF02991	0.432
LIG_LIR_Gen_1	100	107	PF02991	0.338
LIG_LIR_Gen_1	224	232	PF02991	0.493
LIG_LIR_Gen_1	43	54	PF02991	0.479
LIG_LIR_Gen_1	510	521	PF02991	0.532
LIG_LIR_Nem_3	100	106	PF02991	0.348
LIG_LIR_Nem_3	135	140	PF02991	0.426
LIG_LIR_Nem_3	224	230	PF02991	0.567
LIG_LIR_Nem_3	32	38	PF02991	0.592
LIG_LIR_Nem_3	390	394	PF02991	0.565
LIG_LIR_Nem_3	43	49	PF02991	0.619
LIG_LIR_Nem_3	71	75	PF02991	0.421
LIG_LYPXL_yS_3	518	521	PF13949	0.540
LIG_MLH1_MIPbox_1	341	345	PF16413	0.282
LIG_NRBOX	545	551	PF00104	0.346
LIG_PDZ_Class_2	580	585	PF00595	0.401
LIG_Pex14_2	134	138	PF04695	0.307
LIG_Pex14_2	337	341	PF04695	0.249
LIG_Pex14_2	364	368	PF04695	0.343
LIG_SH2_CRK	103	107	PF00017	0.360
LIG_SH2_CRK	294	298	PF00017	0.390
LIG_SH2_NCK_1	294	298	PF00017	0.389
LIG_SH2_NCK_1	46	50	PF00017	0.622
LIG_SH2_PTP2	73	76	PF00017	0.512
LIG_SH2_SRC	582	585	PF00017	0.406
LIG_SH2_STAP1	103	107	PF00017	0.342
LIG_SH2_STAP1	271	275	PF00017	0.446
LIG_SH2_STAP1	46	50	PF00017	0.639
LIG_SH2_STAT3	38	41	PF00017	0.612
LIG_SH2_STAT3	535	538	PF00017	0.649
LIG_SH2_STAT5	103	106	PF00017	0.326
LIG_SH2_STAT5	148	151	PF00017	0.419
LIG_SH2_STAT5	248	251	PF00017	0.575
LIG_SH2_STAT5	46	49	PF00017	0.509
LIG_SH2_STAT5	553	556	PF00017	0.410
LIG_SH2_STAT5	582	585	PF00017	0.418
LIG_SH2_STAT5	73	76	PF00017	0.534
LIG_SH3_3	56	62	PF00018	0.285
LIG_SH3_3	72	78	PF00018	0.318
LIG_SUMO_SIM_par_1	295	301	PF11976	0.389
LIG_TRAF2_1	461	464	PF00917	0.552
LIG_TYR_ITIM	44	49	PF00017	0.475
LIG_TYR_ITIM	580	585	PF00017	0.368
LIG_TYR_ITIM	70	75	PF00017	0.292
LIG_WRC_WIRS_1	341	346	PF05994	0.236
LIG_WRC_WIRS_1	388	393	PF05994	0.642
LIG_WRC_WIRS_1	455	460	PF05994	0.539
MOD_CK1_1	101	107	PF00069	0.367
MOD_CK1_1	241	247	PF00069	0.562
MOD_CK1_1	298	304	PF00069	0.482
MOD_CK1_1	383	389	PF00069	0.604
MOD_CK1_1	539	545	PF00069	0.428
MOD_CK2_1	231	237	PF00069	0.576
MOD_CK2_1	241	247	PF00069	0.481
MOD_CK2_1	3	9	PF00069	0.611
MOD_CK2_1	450	456	PF00069	0.509
MOD_CK2_1	458	464	PF00069	0.502
MOD_CK2_1	467	473	PF00069	0.505
MOD_CK2_1	486	492	PF00069	0.469
MOD_CK2_1	92	98	PF00069	0.392
MOD_GlcNHglycan	186	189	PF01048	0.271
MOD_GlcNHglycan	289	292	PF01048	0.370
MOD_GlcNHglycan	345	348	PF01048	0.404
MOD_GlcNHglycan	368	371	PF01048	0.344
MOD_GlcNHglycan	382	385	PF01048	0.326
MOD_GlcNHglycan	402	405	PF01048	0.421
MOD_GlcNHglycan	420	424	PF01048	0.359
MOD_GlcNHglycan	83	86	PF01048	0.540
MOD_GSK3_1	14	21	PF00069	0.598
MOD_GSK3_1	180	187	PF00069	0.271
MOD_GSK3_1	339	346	PF00069	0.282
MOD_GSK3_1	36	43	PF00069	0.491
MOD_GSK3_1	383	390	PF00069	0.568
MOD_GSK3_1	450	457	PF00069	0.568
MOD_GSK3_1	536	543	PF00069	0.430
MOD_GSK3_1	97	104	PF00069	0.389
MOD_N-GLC_1	301	306	PF02516	0.331
MOD_NEK2_1	238	243	PF00069	0.546
MOD_NEK2_1	287	292	PF00069	0.574
MOD_NEK2_1	29	34	PF00069	0.545
MOD_NEK2_1	345	350	PF00069	0.271
MOD_NEK2_1	36	41	PF00069	0.551
MOD_NEK2_1	380	385	PF00069	0.562
MOD_NEK2_1	450	455	PF00069	0.529
MOD_NEK2_1	544	549	PF00069	0.364
MOD_NEK2_1	55	60	PF00069	0.369
MOD_NEK2_2	340	345	PF00069	0.236
MOD_PIKK_1	231	237	PF00454	0.545
MOD_PKA_2	18	24	PF00069	0.579
MOD_PKA_2	29	35	PF00069	0.535
MOD_PKA_2	408	414	PF00069	0.555
MOD_PKA_2	504	510	PF00069	0.548
MOD_PKA_2	539	545	PF00069	0.388
MOD_Plk_1	301	307	PF00069	0.515
MOD_Plk_1	467	473	PF00069	0.535
MOD_Plk_1	97	103	PF00069	0.297
MOD_Plk_2-3	467	473	PF00069	0.536
MOD_Plk_4	241	247	PF00069	0.521
MOD_Plk_4	29	35	PF00069	0.591
MOD_Plk_4	340	346	PF00069	0.253
MOD_Plk_4	383	389	PF00069	0.597
MOD_Plk_4	430	436	PF00069	0.564
MOD_Plk_4	443	449	PF00069	0.537
MOD_Plk_4	450	456	PF00069	0.423
MOD_Plk_4	467	473	PF00069	0.505
MOD_Plk_4	544	550	PF00069	0.322
MOD_Plk_4	55	61	PF00069	0.362
MOD_Plk_4	68	74	PF00069	0.305
MOD_Plk_4	98	104	PF00069	0.393
MOD_ProDKin_1	481	487	PF00069	0.659
MOD_ProDKin_1	74	80	PF00069	0.365
MOD_SUMO_rev_2	491	496	PF00179	0.487
TRG_DiLeu_BaLyEn_6	517	522	PF01217	0.630
TRG_ENDOCYTIC_2	103	106	PF00928	0.371
TRG_ENDOCYTIC_2	169	172	PF00928	0.404
TRG_ENDOCYTIC_2	294	297	PF00928	0.436
TRG_ENDOCYTIC_2	46	49	PF00928	0.481
TRG_ENDOCYTIC_2	518	521	PF00928	0.591
TRG_ENDOCYTIC_2	582	585	PF00928	0.408
TRG_ENDOCYTIC_2	72	75	PF00928	0.313
TRG_ER_diArg_1	308	310	PF00400	0.531
TRG_ER_diArg_1	406	409	PF00400	0.624
TRG_ER_diArg_1	478	481	PF00400	0.622
TRG_ER_diArg_1	568	570	PF00400	0.358
TRG_Pf-PMV_PEXEL_1	552	557	PF00026	0.581

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7S3	Leptomonas seymouri	64%	100%
A0A1X0NYV6	Trypanosomatidae	46%	100%
A0A3Q8IAW4	Leishmania donovani	93%	100%
A0A422P1M7	Trypanosoma rangeli	47%	100%
A4HGB2	Leishmania braziliensis	81%	100%
A4I3E6	Leishmania infantum	93%	100%
D0A801	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AZN3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5B0S4	Trypanosoma cruzi	48%	100%