DNA-directed RNA polymerases I, II, and III subunit RPABC3

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase B subunit RPB8, putative

Species:

Leishmania major

UniProt:

Q4Q8I8_LEIMA

TriTrypDb:

LmjF.28.0810 , LMJLV39_280013900 , LMJSD75_280013900

Length:

149

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	11
GO:0005654	nucleoplasm	2	2
GO:0005665	RNA polymerase II, core complex	4	11
GO:0005666	RNA polymerase III complex	4	11
GO:0005736	RNA polymerase I complex	4	11
GO:0030880	RNA polymerase complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	11
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	11
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	11
GO:0140535	intracellular protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1990234	transferase complex	3	11

Expansion

Sequence features

Q4Q8I8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8I8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006351	DNA-templated transcription	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0032774	RNA biosynthetic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:0097659	nucleic acid-templated transcription	6	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0016740	transferase activity	2	6
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	6
GO:0016779	nucleotidyltransferase activity	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	23	29	PF00089	0.247
CLV_PCSK_KEX2_1	145	147	PF00082	0.247
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.247
CLV_PCSK_SKI1_1	145	149	PF00082	0.490
DOC_USP7_UBL2_3	71	75	PF12436	0.380
LIG_Actin_WH2_2	10	28	PF00022	0.290
LIG_Actin_WH2_2	117	134	PF00022	0.266
LIG_BIR_II_1	1	5	PF00653	0.465
LIG_BRCT_BRCA1_1	46	50	PF00533	0.380
LIG_FHA_1	20	26	PF00498	0.380
LIG_FHA_1	40	46	PF00498	0.266
LIG_FHA_1	57	63	PF00498	0.275
LIG_FHA_1	9	15	PF00498	0.250
LIG_LIR_Gen_1	138	147	PF02991	0.247
LIG_LIR_Gen_1	22	30	PF02991	0.332
LIG_LIR_Nem_3	138	144	PF02991	0.304
LIG_LIR_Nem_3	22	27	PF02991	0.332
LIG_SH2_PTP2	141	144	PF00017	0.290
LIG_SH2_SRC	77	80	PF00017	0.325
LIG_SH2_STAP1	77	81	PF00017	0.247
LIG_SH2_STAT5	141	144	PF00017	0.275
LIG_SH2_STAT5	24	27	PF00017	0.288
LIG_SH3_3	122	128	PF00018	0.302
LIG_SUMO_SIM_anti_2	56	62	PF11976	0.267
LIG_SUMO_SIM_par_1	4	11	PF11976	0.364
LIG_TYR_ITSM	20	27	PF00017	0.325
LIG_UBA3_1	120	127	PF00899	0.325
MOD_GlcNHglycan	1	4	PF01048	0.474
MOD_GSK3_1	35	42	PF00069	0.348
MOD_N-GLC_2	89	91	PF02516	0.385
MOD_NEK2_1	25	30	PF00069	0.290
MOD_NEK2_1	8	13	PF00069	0.276
MOD_NEK2_2	77	82	PF00069	0.247
MOD_PKA_2	25	31	PF00069	0.247
MOD_PKA_2	56	62	PF00069	0.302
MOD_PKA_2	83	89	PF00069	0.247
MOD_Plk_1	77	83	PF00069	0.247
MOD_Plk_1	8	14	PF00069	0.247
MOD_Plk_2-3	44	50	PF00069	0.325
MOD_Plk_4	19	25	PF00069	0.355
MOD_Plk_4	56	62	PF00069	0.302
MOD_Plk_4	77	83	PF00069	0.247
TRG_ENDOCYTIC_2	141	144	PF00928	0.302
TRG_ENDOCYTIC_2	24	27	PF00928	0.256

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA68	Leptomonas seymouri	93%	100%
A0A0S4INZ3	Bodo saltans	53%	99%
A0A1X0NYD9	Trypanosomatidae	74%	100%
A0A3S7X1E3	Leishmania donovani	100%	100%
A0A422N350	Trypanosoma rangeli	72%	100%
A4HGC3	Leishmania braziliensis	97%	100%
A4I3F6	Leishmania infantum	100%	100%
A8XT81	Caenorhabditis briggsae	30%	100%
D0A7Y4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
E9AZP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	99%	100%
O81097	Arabidopsis thaliana	24%	100%
P20436	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
P52434	Homo sapiens	29%	99%
Q19826	Caenorhabditis elegans	30%	100%
Q54YW8	Dictyostelium discoideum	31%	100%
Q92399	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q923G2	Mus musculus	29%	99%
Q9M1A8	Arabidopsis thaliana	24%	100%