TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania major

UniProt:

Q4Q8A3_LEIMA

TriTrypDb:

LmjF.28.1620 , LMJLV39_280024000 , LMJSD75_280023500

Length:

848

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q8A3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8A3

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	2
GO:0016829	lyase activity	2	2
GO:0016835	carbon-oxygen lyase activity	3	2
GO:0016836	hydro-lyase activity	4	2
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	107	111	PF00656	0.746
CLV_C14_Caspase3-7	439	443	PF00656	0.174
CLV_C14_Caspase3-7	457	461	PF00656	0.302
CLV_NRD_NRD_1	104	106	PF00675	0.536
CLV_NRD_NRD_1	152	154	PF00675	0.554
CLV_NRD_NRD_1	156	158	PF00675	0.556
CLV_NRD_NRD_1	307	309	PF00675	0.403
CLV_NRD_NRD_1	425	427	PF00675	0.321
CLV_NRD_NRD_1	429	431	PF00675	0.323
CLV_NRD_NRD_1	487	489	PF00675	0.268
CLV_NRD_NRD_1	536	538	PF00675	0.437
CLV_NRD_NRD_1	612	614	PF00675	0.281
CLV_NRD_NRD_1	730	732	PF00675	0.618
CLV_NRD_NRD_1	747	749	PF00675	0.490
CLV_NRD_NRD_1	798	800	PF00675	0.503
CLV_NRD_NRD_1	801	803	PF00675	0.536
CLV_NRD_NRD_1	814	816	PF00675	0.682
CLV_NRD_NRD_1	98	100	PF00675	0.539
CLV_PCSK_FUR_1	799	803	PF00082	0.512
CLV_PCSK_FUR_1	812	816	PF00082	0.573
CLV_PCSK_KEX2_1	104	106	PF00082	0.656
CLV_PCSK_KEX2_1	152	154	PF00082	0.686
CLV_PCSK_KEX2_1	156	158	PF00082	0.725
CLV_PCSK_KEX2_1	307	309	PF00082	0.445
CLV_PCSK_KEX2_1	424	426	PF00082	0.312
CLV_PCSK_KEX2_1	429	431	PF00082	0.312
CLV_PCSK_KEX2_1	487	489	PF00082	0.268
CLV_PCSK_KEX2_1	536	538	PF00082	0.483
CLV_PCSK_KEX2_1	747	749	PF00082	0.490
CLV_PCSK_KEX2_1	756	758	PF00082	0.456
CLV_PCSK_KEX2_1	790	792	PF00082	0.439
CLV_PCSK_KEX2_1	800	802	PF00082	0.500
CLV_PCSK_KEX2_1	814	816	PF00082	0.736
CLV_PCSK_KEX2_1	98	100	PF00082	0.691
CLV_PCSK_PC1ET2_1	756	758	PF00082	0.600
CLV_PCSK_PC1ET2_1	790	792	PF00082	0.449
CLV_PCSK_PC1ET2_1	800	802	PF00082	0.472
CLV_PCSK_PC7_1	152	158	PF00082	0.455
CLV_PCSK_PC7_1	425	431	PF00082	0.268
CLV_PCSK_PC7_1	743	749	PF00082	0.489
CLV_PCSK_PC7_1	94	100	PF00082	0.527
CLV_PCSK_SKI1_1	229	233	PF00082	0.716
CLV_PCSK_SKI1_1	336	340	PF00082	0.258
CLV_PCSK_SKI1_1	346	350	PF00082	0.285
CLV_PCSK_SKI1_1	406	410	PF00082	0.281
CLV_PCSK_SKI1_1	487	491	PF00082	0.330
CLV_PCSK_SKI1_1	69	73	PF00082	0.589
CLV_PCSK_SKI1_1	756	760	PF00082	0.528
CLV_Separin_Metazoa	606	610	PF03568	0.268
DEG_APCC_DBOX_1	287	295	PF00400	0.283
DEG_APCC_DBOX_1	335	343	PF00400	0.304
DOC_CDC14_PxL_1	441	449	PF14671	0.279
DOC_CKS1_1	4	9	PF01111	0.443
DOC_CKS1_1	73	78	PF01111	0.494
DOC_CYCLIN_RxL_1	609	621	PF00134	0.362
DOC_CYCLIN_RxL_1	754	763	PF00134	0.547
DOC_MAPK_DCC_7	72	81	PF00069	0.455
DOC_MAPK_gen_1	307	315	PF00069	0.272
DOC_MAPK_gen_1	424	436	PF00069	0.351
DOC_MAPK_MEF2A_6	72	81	PF00069	0.455
DOC_PP1_RVXF_1	754	761	PF00149	0.515
DOC_PP2B_LxvP_1	663	666	PF13499	0.424
DOC_PP2B_LxvP_1	721	724	PF13499	0.516
DOC_PP4_FxxP_1	4	7	PF00568	0.407
DOC_PP4_FxxP_1	604	607	PF00568	0.268
DOC_PP4_FxxP_1	73	76	PF00568	0.581
DOC_USP7_MATH_1	113	117	PF00917	0.555
DOC_USP7_MATH_1	211	215	PF00917	0.750
DOC_USP7_MATH_1	594	598	PF00917	0.412
DOC_USP7_MATH_1	707	711	PF00917	0.682
DOC_USP7_MATH_1	785	789	PF00917	0.594
DOC_USP7_MATH_1	829	833	PF00917	0.517
DOC_WW_Pin1_4	292	297	PF00397	0.274
DOC_WW_Pin1_4	3	8	PF00397	0.414
DOC_WW_Pin1_4	32	37	PF00397	0.398
DOC_WW_Pin1_4	72	77	PF00397	0.500
DOC_WW_Pin1_4	803	808	PF00397	0.621
DOC_WW_Pin1_4	815	820	PF00397	0.592
LIG_14-3-3_CanoR_1	271	276	PF00244	0.414
LIG_14-3-3_CanoR_1	369	373	PF00244	0.268
LIG_14-3-3_CanoR_1	487	494	PF00244	0.264
LIG_14-3-3_CanoR_1	632	636	PF00244	0.285
LIG_14-3-3_CanoR_1	814	819	PF00244	0.676
LIG_Actin_WH2_2	460	478	PF00022	0.412
LIG_AP2alpha_2	728	730	PF02296	0.624
LIG_BIR_III_2	206	210	PF00653	0.461
LIG_BIR_III_2	442	446	PF00653	0.262
LIG_BRCT_BRCA1_1	67	71	PF00533	0.703
LIG_BRCT_BRCA1_1	842	846	PF00533	0.593
LIG_CaM_IQ_9	120	135	PF13499	0.552
LIG_CtBP_PxDLS_1	615	619	PF00389	0.283
LIG_deltaCOP1_diTrp_1	538	543	PF00928	0.441
LIG_eIF4E_1	320	326	PF01652	0.302
LIG_FHA_1	193	199	PF00498	0.718
LIG_FHA_1	320	326	PF00498	0.279
LIG_FHA_1	502	508	PF00498	0.283
LIG_FHA_1	657	663	PF00498	0.553
LIG_FHA_1	73	79	PF00498	0.713
LIG_FHA_1	778	784	PF00498	0.629
LIG_FHA_1	828	834	PF00498	0.774
LIG_FHA_2	169	175	PF00498	0.569
LIG_FHA_2	36	42	PF00498	0.651
LIG_FHA_2	397	403	PF00498	0.287
LIG_FHA_2	417	423	PF00498	0.145
LIG_FHA_2	437	443	PF00498	0.240
LIG_FHA_2	455	461	PF00498	0.302
LIG_FHA_2	529	535	PF00498	0.327
LIG_FHA_2	540	546	PF00498	0.334
LIG_FHA_2	554	560	PF00498	0.327
LIG_FHA_2	681	687	PF00498	0.527
LIG_LIR_Apic_2	603	607	PF02991	0.269
LIG_LIR_Apic_2	70	76	PF02991	0.530
LIG_LIR_Gen_1	378	389	PF02991	0.294
LIG_LIR_Gen_1	538	547	PF02991	0.412
LIG_LIR_LC3C_4	41	46	PF02991	0.386
LIG_LIR_Nem_3	119	125	PF02991	0.547
LIG_LIR_Nem_3	358	364	PF02991	0.329
LIG_LIR_Nem_3	378	384	PF02991	0.247
LIG_LIR_Nem_3	477	482	PF02991	0.327
LIG_LIR_Nem_3	538	543	PF02991	0.441
LIG_LIR_Nem_3	566	570	PF02991	0.327
LIG_LIR_Nem_3	667	673	PF02991	0.530
LIG_LIR_Nem_3	686	691	PF02991	0.407
LIG_LIR_Nem_3	835	839	PF02991	0.632
LIG_MYND_1	160	164	PF01753	0.484
LIG_NRBOX	404	410	PF00104	0.268
LIG_PCNA_PIPBox_1	574	583	PF02747	0.302
LIG_PCNA_yPIPBox_3	574	587	PF02747	0.422
LIG_PCNA_yPIPBox_3	637	649	PF02747	0.268
LIG_Pex14_1	539	543	PF04695	0.412
LIG_Pex14_2	12	16	PF04695	0.366
LIG_Pex14_2	331	335	PF04695	0.268
LIG_Pex14_2	391	395	PF04695	0.283
LIG_SH2_CRK	581	585	PF00017	0.395
LIG_SH2_CRK	640	644	PF00017	0.268
LIG_SH2_CRK	793	797	PF00017	0.598
LIG_SH2_GRB2like	640	643	PF00017	0.268
LIG_SH2_NCK_1	640	644	PF00017	0.283
LIG_SH2_SRC	622	625	PF00017	0.276
LIG_SH2_SRC	640	643	PF00017	0.268
LIG_SH2_SRC	691	694	PF00017	0.596
LIG_SH2_STAP1	622	626	PF00017	0.395
LIG_SH2_STAP1	640	644	PF00017	0.268
LIG_SH2_STAP1	793	797	PF00017	0.598
LIG_SH2_STAT3	365	368	PF00017	0.293
LIG_SH2_STAT3	580	583	PF00017	0.283
LIG_SH2_STAT5	243	246	PF00017	0.269
LIG_SH2_STAT5	281	284	PF00017	0.283
LIG_SH2_STAT5	398	401	PF00017	0.283
LIG_SH2_STAT5	479	482	PF00017	0.350
LIG_SH2_STAT5	555	558	PF00017	0.362
LIG_SH2_STAT5	680	683	PF00017	0.453
LIG_SH2_STAT5	803	806	PF00017	0.446
LIG_SH3_1	157	163	PF00018	0.525
LIG_SH3_3	157	163	PF00018	0.525
LIG_SH3_3	321	327	PF00018	0.309
LIG_SH3_3	339	345	PF00018	0.347
LIG_SH3_3	557	563	PF00018	0.269
LIG_SH3_3	604	610	PF00018	0.298
LIG_SH3_3	778	784	PF00018	0.541
LIG_SUMO_SIM_anti_2	658	664	PF11976	0.488
LIG_SUMO_SIM_par_1	614	619	PF11976	0.334
LIG_TRAF2_1	116	119	PF00917	0.484
LIG_TRAF2_1	38	41	PF00917	0.384
LIG_UBA3_1	78	84	PF00899	0.463
LIG_WRC_WIRS_1	494	499	PF05994	0.412
LIG_WRC_WIRS_1	540	545	PF05994	0.402
LIG_WRC_WIRS_1	833	838	PF05994	0.703
LIG_WW_3	606	610	PF00397	0.268
MOD_CDK_SPK_2	32	37	PF00069	0.398
MOD_CDK_SPK_2	803	808	PF00069	0.627
MOD_CK1_1	117	123	PF00069	0.675
MOD_CK1_1	183	189	PF00069	0.674
MOD_CK1_1	316	322	PF00069	0.300
MOD_CK1_1	35	41	PF00069	0.622
MOD_CK1_1	763	769	PF00069	0.461
MOD_CK1_1	821	827	PF00069	0.756
MOD_CK1_1	832	838	PF00069	0.635
MOD_CK2_1	113	119	PF00069	0.678
MOD_CK2_1	168	174	PF00069	0.568
MOD_CK2_1	35	41	PF00069	0.627
MOD_CK2_1	401	407	PF00069	0.292
MOD_CK2_1	416	422	PF00069	0.327
MOD_CK2_1	528	534	PF00069	0.395
MOD_DYRK1A_RPxSP_1	815	819	PF00069	0.619
MOD_GlcNHglycan	182	185	PF01048	0.655
MOD_GlcNHglycan	195	198	PF01048	0.572
MOD_GlcNHglycan	220	223	PF01048	0.692
MOD_GlcNHglycan	452	455	PF01048	0.343
MOD_GlcNHglycan	598	601	PF01048	0.362
MOD_GlcNHglycan	623	627	PF01048	0.174
MOD_GlcNHglycan	650	653	PF01048	0.453
MOD_GlcNHglycan	787	790	PF01048	0.598
MOD_GlcNHglycan	826	829	PF01048	0.721
MOD_GSK3_1	113	120	PF00069	0.714
MOD_GSK3_1	179	186	PF00069	0.642
MOD_GSK3_1	188	195	PF00069	0.654
MOD_GSK3_1	31	38	PF00069	0.511
MOD_GSK3_1	450	457	PF00069	0.373
MOD_GSK3_1	483	490	PF00069	0.302
MOD_GSK3_1	627	634	PF00069	0.377
MOD_GSK3_1	759	766	PF00069	0.656
MOD_GSK3_1	813	820	PF00069	0.763
MOD_GSK3_1	823	830	PF00069	0.733
MOD_GSK3_1	840	847	PF00069	0.483
MOD_LATS_1	269	275	PF00433	0.412
MOD_N-GLC_1	292	297	PF02516	0.289
MOD_N-GLC_1	396	401	PF02516	0.362
MOD_N-GLC_1	707	712	PF02516	0.701
MOD_N-GLC_1	785	790	PF02516	0.589
MOD_NEK2_1	244	249	PF00069	0.440
MOD_NEK2_1	648	653	PF00069	0.526
MOD_NEK2_1	71	76	PF00069	0.633
MOD_NEK2_1	759	764	PF00069	0.498
MOD_NEK2_1	813	818	PF00069	0.681
MOD_NEK2_2	627	632	PF00069	0.334
MOD_PIKK_1	416	422	PF00454	0.334
MOD_PIKK_1	735	741	PF00454	0.635
MOD_PIKK_1	763	769	PF00454	0.602
MOD_PK_1	271	277	PF00069	0.412
MOD_PK_1	340	346	PF00069	0.334
MOD_PKA_1	487	493	PF00069	0.268
MOD_PKA_1	801	807	PF00069	0.712
MOD_PKA_1	814	820	PF00069	0.734
MOD_PKA_2	193	199	PF00069	0.581
MOD_PKA_2	233	239	PF00069	0.456
MOD_PKA_2	368	374	PF00069	0.294
MOD_PKA_2	487	493	PF00069	0.268
MOD_PKA_2	545	551	PF00069	0.444
MOD_PKA_2	553	559	PF00069	0.412
MOD_PKA_2	631	637	PF00069	0.305
MOD_PKA_2	742	748	PF00069	0.604
MOD_PKA_2	801	807	PF00069	0.635
MOD_PKA_2	813	819	PF00069	0.690
MOD_PKA_2	97	103	PF00069	0.696
MOD_PKB_1	216	224	PF00069	0.484
MOD_PKB_1	799	807	PF00069	0.483
MOD_PKB_1	812	820	PF00069	0.645
MOD_Plk_1	117	123	PF00069	0.643
MOD_Plk_1	316	322	PF00069	0.283
MOD_Plk_1	340	346	PF00069	0.398
MOD_Plk_1	401	407	PF00069	0.302
MOD_Plk_1	616	622	PF00069	0.412
MOD_Plk_1	760	766	PF00069	0.587
MOD_Plk_4	118	124	PF00069	0.580
MOD_Plk_4	493	499	PF00069	0.277
MOD_Plk_4	658	664	PF00069	0.527
MOD_Plk_4	77	83	PF00069	0.571
MOD_ProDKin_1	292	298	PF00069	0.274
MOD_ProDKin_1	3	9	PF00069	0.410
MOD_ProDKin_1	32	38	PF00069	0.388
MOD_ProDKin_1	72	78	PF00069	0.502
MOD_ProDKin_1	803	809	PF00069	0.626
MOD_ProDKin_1	815	821	PF00069	0.590
MOD_SUMO_for_1	225	228	PF00179	0.558
MOD_SUMO_for_1	83	86	PF00179	0.503
MOD_SUMO_rev_2	68	74	PF00179	0.753
MOD_SUMO_rev_2	705	714	PF00179	0.602
MOD_SUMO_rev_2	749	758	PF00179	0.559
TRG_DiLeu_BaEn_1	40	45	PF01217	0.386
TRG_DiLeu_BaLyEn_6	74	79	PF01217	0.475
TRG_DiLeu_BaLyEn_6	754	759	PF01217	0.515
TRG_ENDOCYTIC_2	479	482	PF00928	0.362
TRG_ENDOCYTIC_2	581	584	PF00928	0.302
TRG_ENDOCYTIC_2	640	643	PF00928	0.268
TRG_ENDOCYTIC_2	793	796	PF00928	0.599
TRG_ER_diArg_1	127	130	PF00400	0.724
TRG_ER_diArg_1	151	153	PF00400	0.634
TRG_ER_diArg_1	288	291	PF00400	0.271
TRG_ER_diArg_1	306	308	PF00400	0.426
TRG_ER_diArg_1	423	426	PF00400	0.312
TRG_ER_diArg_1	428	430	PF00400	0.312
TRG_ER_diArg_1	487	489	PF00400	0.268
TRG_ER_diArg_1	552	555	PF00400	0.334
TRG_ER_diArg_1	799	802	PF00400	0.488
TRG_ER_diArg_1	811	814	PF00400	0.611
TRG_NES_CRM1_1	280	292	PF08389	0.327
TRG_NLS_Bipartite_1	790	804	PF00514	0.497
TRG_NLS_MonoCore_2	798	803	PF00514	0.624
TRG_NLS_MonoExtN_4	799	804	PF00514	0.592
TRG_Pf-PMV_PEXEL_1	403	407	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	429	433	PF00026	0.368
TRG_Pf-PMV_PEXEL_1	487	492	PF00026	0.315
TRG_Pf-PMV_PEXEL_1	514	519	PF00026	0.334
TRG_Pf-PMV_PEXEL_1	65	70	PF00026	0.576
TRG_Pf-PMV_PEXEL_1	653	658	PF00026	0.454
TRG_Pf-PMV_PEXEL_1	757	761	PF00026	0.531

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P820	Leptomonas seymouri	65%	85%
A0A0S4K0L8	Bodo saltans	45%	84%
A0A1X0NQX7	Trypanosomatidae	57%	92%
A0A3Q8II50	Leishmania donovani	93%	100%
A0A3S5ISD4	Trypanosoma rangeli	60%	96%
A4HGL5	Leishmania braziliensis	84%	100%
A4I3P1	Leishmania infantum	95%	100%
D0A8C3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	92%
E9AZY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
V5BFB8	Trypanosoma cruzi	54%	92%