Replication protein A subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Replication factor A protein 1

Species:

Leishmania major

UniProt:

Q4Q884_LEIMA

TriTrypDb:

LmjF.28.1820 , LMJLV39_280026000 , LMJSD75_280025500

Length:

467

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 4
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005662	DNA replication factor A complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0034399	nuclear periphery	2	2
GO:0110165	cellular anatomical entity	1	7
GO:0140513	nuclear protein-containing complex	2	2
GO:0005634	nucleus	5	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5

Expansion

Sequence features

Q4Q884

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q884

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	2
GO:0000724	double-strand break repair via homologous recombination	7	2
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006259	DNA metabolic process	4	7
GO:0006268	DNA unwinding involved in DNA replication	9	2
GO:0006278	RNA-templated DNA biosynthetic process	6	2
GO:0006281	DNA repair	5	7
GO:0006289	nucleotide-excision repair	6	2
GO:0006302	double-strand break repair	6	2
GO:0006310	DNA recombination	5	7
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0006950	response to stress	2	7
GO:0006974	DNA damage response	4	7
GO:0006996	organelle organization	4	2
GO:0007004	telomere maintenance via telomerase	7	2
GO:0007049	cell cycle	2	2
GO:0008152	metabolic process	1	7
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0009987	cellular process	1	7
GO:0010833	telomere maintenance via telomere lengthening	6	2
GO:0016043	cellular component organization	3	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0022414	reproductive process	1	2
GO:0032200	telomere organization	6	2
GO:0032392	DNA geometric change	7	2
GO:0032508	DNA duplex unwinding	8	2
GO:0033554	cellular response to stress	3	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0044249	cellular biosynthetic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	7
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0046483	heterocycle metabolic process	3	7
GO:0050896	response to stimulus	1	7
GO:0051276	chromosome organization	5	2
GO:0051321	meiotic cell cycle	2	2
GO:0051716	cellular response to stimulus	2	7
GO:0071103	DNA conformation change	6	2
GO:0071704	organic substance metabolic process	2	7
GO:0071840	cellular component organization or biogenesis	2	2
GO:0071897	DNA biosynthetic process	5	2
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2
GO:0006260	DNA replication	5	5

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	7
GO:0003684	damaged DNA binding	5	2
GO:0003697	single-stranded DNA binding	5	2
GO:0005488	binding	1	7
GO:0042162	telomeric DNA binding	6	2
GO:0043047	single-stranded telomeric DNA binding	7	2
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0043565	sequence-specific DNA binding	5	2
GO:0046872	metal ion binding	4	7
GO:0097159	organic cyclic compound binding	2	7
GO:0098847	sequence-specific single stranded DNA binding	6	2
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	273	275	PF00675	0.467
CLV_NRD_NRD_1	380	382	PF00675	0.345
CLV_NRD_NRD_1	446	448	PF00675	0.553
CLV_NRD_NRD_1	93	95	PF00675	0.311
CLV_PCSK_KEX2_1	172	174	PF00082	0.345
CLV_PCSK_KEX2_1	379	381	PF00082	0.345
CLV_PCSK_KEX2_1	445	447	PF00082	0.563
CLV_PCSK_KEX2_1	93	95	PF00082	0.311
CLV_PCSK_PC1ET2_1	172	174	PF00082	0.345
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.345
CLV_PCSK_PC7_1	168	174	PF00082	0.345
CLV_PCSK_SKI1_1	173	177	PF00082	0.345
CLV_PCSK_SKI1_1	207	211	PF00082	0.345
CLV_PCSK_SKI1_1	239	243	PF00082	0.501
CLV_PCSK_SKI1_1	287	291	PF00082	0.639
CLV_PCSK_SKI1_1	318	322	PF00082	0.355
CLV_PCSK_SKI1_1	337	341	PF00082	0.407
CLV_Separin_Metazoa	458	462	PF03568	0.553
DEG_APCC_DBOX_1	273	281	PF00400	0.460
DEG_Nend_UBRbox_3	1	3	PF02207	0.598
DOC_MAPK_gen_1	287	296	PF00069	0.444
DOC_MAPK_gen_1	396	405	PF00069	0.345
DOC_MAPK_gen_1	423	431	PF00069	0.345
DOC_MAPK_MEF2A_6	128	136	PF00069	0.433
DOC_MAPK_MEF2A_6	344	351	PF00069	0.345
DOC_MAPK_MEF2A_6	399	407	PF00069	0.360
DOC_USP7_MATH_1	11	15	PF00917	0.445
DOC_USP7_MATH_1	438	442	PF00917	0.550
DOC_USP7_UBL2_3	172	176	PF12436	0.345
DOC_WW_Pin1_4	13	18	PF00397	0.452
DOC_WW_Pin1_4	337	342	PF00397	0.410
LIG_14-3-3_CanoR_1	108	114	PF00244	0.444
LIG_14-3-3_CanoR_1	26	35	PF00244	0.557
LIG_14-3-3_CanoR_1	423	429	PF00244	0.345
LIG_14-3-3_CanoR_1	430	437	PF00244	0.499
LIG_14-3-3_CanoR_1	60	64	PF00244	0.511
LIG_Actin_WH2_2	196	212	PF00022	0.345
LIG_APCC_ABBA_1	360	365	PF00400	0.345
LIG_APCC_ABBAyCdc20_2	60	66	PF00400	0.511
LIG_BIR_III_2	311	315	PF00653	0.345
LIG_FHA_1	119	125	PF00498	0.493
LIG_FHA_1	14	20	PF00498	0.454
LIG_FHA_1	179	185	PF00498	0.345
LIG_FHA_1	240	246	PF00498	0.445
LIG_FHA_1	350	356	PF00498	0.345
LIG_FHA_2	164	170	PF00498	0.345
LIG_FHA_2	223	229	PF00498	0.345
LIG_FHA_2	415	421	PF00498	0.345
LIG_FHA_2	438	444	PF00498	0.546
LIG_FHA_2	452	458	PF00498	0.494
LIG_FHA_2	49	55	PF00498	0.511
LIG_GBD_Chelix_1	145	153	PF00786	0.345
LIG_LIR_Apic_2	37	41	PF02991	0.511
LIG_LIR_Gen_1	292	301	PF02991	0.345
LIG_LIR_Gen_1	356	366	PF02991	0.345
LIG_LIR_Gen_1	49	58	PF02991	0.511
LIG_LIR_Nem_3	213	217	PF02991	0.345
LIG_LIR_Nem_3	292	296	PF02991	0.345
LIG_LIR_Nem_3	356	362	PF02991	0.345
LIG_LIR_Nem_3	432	437	PF02991	0.515
LIG_LIR_Nem_3	49	53	PF02991	0.511
LIG_LIR_Nem_3	69	74	PF02991	0.424
LIG_PCNA_yPIPBox_3	138	146	PF02747	0.345
LIG_Pex14_1	359	363	PF04695	0.345
LIG_SH2_CRK	129	133	PF00017	0.430
LIG_SH2_PTP2	293	296	PF00017	0.345
LIG_SH2_SRC	293	296	PF00017	0.345
LIG_SH2_STAP1	102	106	PF00017	0.511
LIG_SH2_STAT3	302	305	PF00017	0.345
LIG_SH2_STAT5	244	247	PF00017	0.469
LIG_SH2_STAT5	276	279	PF00017	0.471
LIG_SH2_STAT5	293	296	PF00017	0.345
LIG_SH2_STAT5	302	305	PF00017	0.345
LIG_SH2_STAT5	345	348	PF00017	0.345
LIG_SH2_STAT5	388	391	PF00017	0.345
LIG_SH2_STAT5	82	85	PF00017	0.511
LIG_SH3_4	40	47	PF00018	0.511
LIG_SUMO_SIM_anti_2	144	150	PF11976	0.345
LIG_TRAF2_1	417	420	PF00917	0.345
LIG_TRAF2_1	440	443	PF00917	0.517
LIG_WRC_WIRS_1	211	216	PF05994	0.345
LIG_WRC_WIRS_1	47	52	PF05994	0.511
LIG_WRC_WIRS_1	68	73	PF05994	0.511
MOD_CDK_SPxxK_3	337	344	PF00069	0.410
MOD_CK1_1	159	165	PF00069	0.345
MOD_CK1_1	254	260	PF00069	0.525
MOD_CK1_1	412	418	PF00069	0.279
MOD_CK1_1	441	447	PF00069	0.543
MOD_CK2_1	163	169	PF00069	0.345
MOD_CK2_1	414	420	PF00069	0.365
MOD_CK2_1	437	443	PF00069	0.532
MOD_Cter_Amidation	272	275	PF01082	0.476
MOD_GlcNHglycan	263	266	PF01048	0.658
MOD_GlcNHglycan	54	58	PF01048	0.311
MOD_GlcNHglycan	83	86	PF01048	0.311
MOD_GSK3_1	102	109	PF00069	0.571
MOD_GSK3_1	159	166	PF00069	0.345
MOD_GSK3_1	215	222	PF00069	0.345
MOD_GSK3_1	250	257	PF00069	0.507
MOD_GSK3_1	30	37	PF00069	0.511
MOD_GSK3_1	335	342	PF00069	0.347
MOD_GSK3_1	345	352	PF00069	0.361
MOD_GSK3_1	410	417	PF00069	0.345
MOD_GSK3_1	437	444	PF00069	0.549
MOD_N-GLC_1	250	255	PF02516	0.517
MOD_N-GLC_1	353	358	PF02516	0.370
MOD_NEK2_1	219	224	PF00069	0.345
MOD_NEK2_1	424	429	PF00069	0.345
MOD_NEK2_1	48	53	PF00069	0.511
MOD_NEK2_2	358	363	PF00069	0.410
MOD_PIKK_1	159	165	PF00454	0.345
MOD_PIKK_1	254	260	PF00454	0.525
MOD_PIKK_1	339	345	PF00454	0.345
MOD_PIKK_1	353	359	PF00454	0.345
MOD_PIKK_1	40	46	PF00454	0.511
MOD_PKA_1	318	324	PF00069	0.345
MOD_PKA_1	93	99	PF00069	0.511
MOD_PKA_2	107	113	PF00069	0.443
MOD_PKA_2	27	33	PF00069	0.557
MOD_PKA_2	424	430	PF00069	0.345
MOD_PKA_2	59	65	PF00069	0.511
MOD_PKA_2	93	99	PF00069	0.511
MOD_Plk_1	102	108	PF00069	0.566
MOD_Plk_1	156	162	PF00069	0.345
MOD_Plk_1	184	190	PF00069	0.345
MOD_Plk_1	250	256	PF00069	0.512
MOD_Plk_1	335	341	PF00069	0.345
MOD_Plk_2-3	141	147	PF00069	0.345
MOD_Plk_4	141	147	PF00069	0.345
MOD_Plk_4	184	190	PF00069	0.345
MOD_Plk_4	199	205	PF00069	0.345
MOD_Plk_4	239	245	PF00069	0.445
MOD_Plk_4	358	364	PF00069	0.360
MOD_Plk_4	424	430	PF00069	0.345
MOD_Plk_4	59	65	PF00069	0.511
MOD_ProDKin_1	13	19	PF00069	0.452
MOD_ProDKin_1	337	343	PF00069	0.410
MOD_SUMO_for_1	289	292	PF00179	0.464
MOD_SUMO_for_1	407	410	PF00179	0.345
MOD_SUMO_rev_2	191	195	PF00179	0.325
MOD_SUMO_rev_2	233	240	PF00179	0.465
TRG_DiLeu_BaEn_1	141	146	PF01217	0.345
TRG_DiLeu_BaEn_1	458	463	PF01217	0.552
TRG_DiLeu_BaEn_4	419	425	PF01217	0.345
TRG_DiLeu_BaLyEn_6	70	75	PF01217	0.557
TRG_DiLeu_LyEn_5	458	463	PF01217	0.552
TRG_ENDOCYTIC_2	129	132	PF00928	0.427
TRG_ENDOCYTIC_2	293	296	PF00928	0.345
TRG_ER_diArg_1	25	28	PF00400	0.521
TRG_ER_diArg_1	403	406	PF00400	0.345
TRG_ER_diArg_1	423	426	PF00400	0.224
TRG_ER_diArg_1	445	447	PF00400	0.566
TRG_ER_diArg_1	93	95	PF00400	0.511
TRG_NLS_MonoExtC_3	378	384	PF00514	0.345
TRG_Pf-PMV_PEXEL_1	275	279	PF00026	0.457
TRG_Pf-PMV_PEXEL_1	287	292	PF00026	0.471
TRG_Pf-PMV_PEXEL_1	446	450	PF00026	0.601

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P825	Leptomonas seymouri	92%	100%
A0A0S4J8L0	Bodo saltans	56%	97%
A0A1X0NQV3	Trypanosomatidae	68%	99%
A0A3R7KN38	Trypanosoma rangeli	73%	100%
A0A3S7X1P8	Leishmania donovani	99%	100%
A4HGN5	Leishmania braziliensis	97%	100%
A4I3Q8	Leishmania infantum	99%	100%
D0A8A0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
D8UYN9	Tetrahymena thermophila (strain SB210)	25%	70%
E9B001	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O97472	Caenorhabditis briggsae	29%	71%
P22336	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	75%
P27694	Homo sapiens	35%	76%
Q01588	Xenopus laevis	33%	77%
Q10Q08	Oryza sativa subsp. japonica	35%	74%
Q19537	Caenorhabditis elegans	30%	71%
Q23696	Crithidia fasciculata	92%	100%
Q24492	Drosophila melanogaster	35%	77%
Q5FW17	Xenopus tropicalis	33%	77%
Q5R7Q4	Pongo abelii	35%	76%
Q5ZJJ2	Gallus gallus	34%	76%
Q6NY74	Danio rerio	34%	78%
Q6YZ49	Oryza sativa subsp. japonica	33%	71%
Q8VEE4	Mus musculus	34%	75%
Q92372	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	77%
Q9FME0	Arabidopsis thaliana	33%	74%
Q9SD82	Arabidopsis thaliana	36%	77%
Q9SKI4	Arabidopsis thaliana	32%	73%
V5DBB0	Trypanosoma cruzi	74%	100%