Adenine DNA glycosylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

A/G-specific adenine glycosylase, putative

Species:

Leishmania major

UniProt:

Q4Q851_LEIMA

TriTrypDb:

LmjF.28.2140 , LMJLV39_280029500 , LMJSD75_280029100

Length:

501

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q851

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q851

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006284	base-excision repair	6	12
GO:0006298	mismatch repair	6	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000700	mismatch base pair DNA N-glycosylase activity	5	12
GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity	6	12
GO:0000702	oxidized base lesion DNA N-glycosylase activity	5	2
GO:0003676	nucleic acid binding	3	5
GO:0003677	DNA binding	4	5
GO:0003684	damaged DNA binding	5	2
GO:0003690	double-stranded DNA binding	5	2
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008534	oxidized purine nucleobase lesion DNA N-glycosylase activity	6	2
GO:0016787	hydrolase activity	2	12
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	12
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	12
GO:0019104	DNA N-glycosylase activity	4	12
GO:0030983	mismatched DNA binding	6	2
GO:0032356	oxidized DNA binding	6	2
GO:0032357	oxidized purine DNA binding	7	2
GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity	7	2
GO:0035485	adenine/guanine mispair binding	7	2
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0097159	organic cyclic compound binding	2	5
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	5
GO:0004518	nuclease activity	4	2
GO:0004519	endonuclease activity	5	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	482	486	PF00656	0.533
CLV_NRD_NRD_1	134	136	PF00675	0.249
CLV_NRD_NRD_1	139	141	PF00675	0.251
CLV_NRD_NRD_1	306	308	PF00675	0.430
CLV_NRD_NRD_1	324	326	PF00675	0.325
CLV_NRD_NRD_1	330	332	PF00675	0.313
CLV_PCSK_KEX2_1	107	109	PF00082	0.231
CLV_PCSK_KEX2_1	134	136	PF00082	0.249
CLV_PCSK_KEX2_1	303	305	PF00082	0.549
CLV_PCSK_KEX2_1	324	326	PF00082	0.325
CLV_PCSK_KEX2_1	330	332	PF00082	0.313
CLV_PCSK_KEX2_1	414	416	PF00082	0.569
CLV_PCSK_KEX2_1	477	479	PF00082	0.634
CLV_PCSK_PC1ET2_1	107	109	PF00082	0.231
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.549
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.583
CLV_PCSK_PC1ET2_1	477	479	PF00082	0.634
CLV_PCSK_SKI1_1	141	145	PF00082	0.271
CLV_PCSK_SKI1_1	297	301	PF00082	0.576
CLV_PCSK_SKI1_1	386	390	PF00082	0.346
CLV_PCSK_SKI1_1	437	441	PF00082	0.484
CLV_PCSK_SKI1_1	453	457	PF00082	0.296
DEG_APCC_DBOX_1	452	460	PF00400	0.427
DEG_SCF_FBW7_1	16	21	PF00400	0.660
DOC_CKS1_1	15	20	PF01111	0.634
DOC_CYCLIN_yCln2_LP_2	361	367	PF00134	0.372
DOC_MAPK_gen_1	303	311	PF00069	0.425
DOC_PP2B_LxvP_1	169	172	PF13499	0.537
DOC_PP4_FxxP_1	187	190	PF00568	0.500
DOC_SPAK_OSR1_1	325	329	PF12202	0.338
DOC_USP7_MATH_1	176	180	PF00917	0.465
DOC_USP7_MATH_1	18	22	PF00917	0.660
DOC_USP7_MATH_1	181	185	PF00917	0.435
DOC_USP7_MATH_1	190	194	PF00917	0.407
DOC_USP7_MATH_1	2	6	PF00917	0.493
DOC_USP7_MATH_1	234	238	PF00917	0.647
DOC_USP7_MATH_1	302	306	PF00917	0.502
DOC_USP7_MATH_1	54	58	PF00917	0.706
DOC_USP7_UBL2_3	457	461	PF12436	0.389
DOC_WW_Pin1_4	14	19	PF00397	0.580
DOC_WW_Pin1_4	230	235	PF00397	0.588
DOC_WW_Pin1_4	485	490	PF00397	0.732
LIG_14-3-3_CanoR_1	386	396	PF00244	0.333
LIG_14-3-3_CanoR_1	50	54	PF00244	0.525
LIG_14-3-3_CanoR_1	70	80	PF00244	0.580
LIG_Actin_WH2_2	191	208	PF00022	0.389
LIG_BIR_III_4	406	410	PF00653	0.372
LIG_BRCT_BRCA1_1	183	187	PF00533	0.451
LIG_BRCT_BRCA1_2	183	189	PF00533	0.389
LIG_deltaCOP1_diTrp_1	14	22	PF00928	0.589
LIG_FHA_1	154	160	PF00498	0.389
LIG_FHA_1	234	240	PF00498	0.591
LIG_FHA_2	117	123	PF00498	0.556
LIG_FHA_2	91	97	PF00498	0.466
LIG_LIR_Apic_2	14	18	PF02991	0.638
LIG_LIR_Apic_2	184	190	PF02991	0.565
LIG_LIR_Gen_1	377	385	PF02991	0.437
LIG_LIR_Nem_3	26	31	PF02991	0.442
LIG_LIR_Nem_3	342	346	PF02991	0.337
LIG_LIR_Nem_3	377	381	PF02991	0.437
LIG_LIR_Nem_3	75	81	PF02991	0.520
LIG_LRP6_Inhibitor_1	194	200	PF00058	0.337
LIG_PCNA_yPIPBox_3	29	39	PF02747	0.428
LIG_PCNA_yPIPBox_3	330	341	PF02747	0.372
LIG_Pex14_1	105	109	PF04695	0.451
LIG_RPA_C_Fungi	319	331	PF08784	0.411
LIG_SH2_CRK	28	32	PF00017	0.417
LIG_SH2_NCK_1	177	181	PF00017	0.319
LIG_SH2_STAP1	177	181	PF00017	0.319
LIG_SH2_STAT5	102	105	PF00017	0.268
LIG_SH2_STAT5	138	141	PF00017	0.290
LIG_SH2_STAT5	145	148	PF00017	0.299
LIG_SH2_STAT5	177	180	PF00017	0.294
LIG_SH3_3	156	162	PF00018	0.295
LIG_SH3_3	210	216	PF00018	0.315
LIG_SH3_3	255	261	PF00018	0.380
LIG_SH3_3	326	332	PF00018	0.360
LIG_SH3_3	398	404	PF00018	0.410
LIG_TRAF2_1	163	166	PF00917	0.423
LIG_TRAF2_1	30	33	PF00917	0.506
LIG_TRAF2_1	479	482	PF00917	0.726
LIG_UBA3_1	455	461	PF00899	0.431
LIG_WRC_WIRS_1	19	24	PF05994	0.573
MOD_CDC14_SPxK_1	488	491	PF00782	0.758
MOD_CDK_SPxK_1	485	491	PF00069	0.749
MOD_CDK_SPxxK_3	485	492	PF00069	0.751
MOD_CK1_1	21	27	PF00069	0.605
MOD_CK1_1	233	239	PF00069	0.660
MOD_CK1_1	483	489	PF00069	0.640
MOD_CK1_1	72	78	PF00069	0.544
MOD_CK1_1	90	96	PF00069	0.160
MOD_CK2_1	116	122	PF00069	0.423
MOD_CK2_1	160	166	PF00069	0.363
MOD_CK2_1	27	33	PF00069	0.497
MOD_CK2_1	460	466	PF00069	0.527
MOD_CK2_1	90	96	PF00069	0.368
MOD_Cter_Amidation	475	478	PF01082	0.680
MOD_GlcNHglycan	178	181	PF01048	0.365
MOD_GlcNHglycan	236	239	PF01048	0.620
MOD_GlcNHglycan	243	247	PF01048	0.433
MOD_GlcNHglycan	261	264	PF01048	0.284
MOD_GlcNHglycan	278	281	PF01048	0.441
MOD_GlcNHglycan	304	307	PF01048	0.524
MOD_GlcNHglycan	314	318	PF01048	0.496
MOD_GlcNHglycan	348	351	PF01048	0.407
MOD_GlcNHglycan	4	7	PF01048	0.730
MOD_GlcNHglycan	56	59	PF01048	0.714
MOD_GlcNHglycan	60	63	PF01048	0.693
MOD_GlcNHglycan	71	74	PF01048	0.495
MOD_GSK3_1	14	21	PF00069	0.670
MOD_GSK3_1	176	183	PF00069	0.367
MOD_GSK3_1	230	237	PF00069	0.629
MOD_GSK3_1	342	349	PF00069	0.311
MOD_GSK3_1	481	488	PF00069	0.650
MOD_GSK3_1	54	61	PF00069	0.594
MOD_GSK3_1	68	75	PF00069	0.648
MOD_GSK3_1	83	90	PF00069	0.206
MOD_N-GLC_1	346	351	PF02516	0.440
MOD_NEK2_1	254	259	PF00069	0.476
MOD_NEK2_1	266	271	PF00069	0.376
MOD_NEK2_1	315	320	PF00069	0.468
MOD_PIKK_1	161	167	PF00454	0.391
MOD_PIKK_1	87	93	PF00454	0.303
MOD_PK_1	108	114	PF00069	0.432
MOD_PK_1	461	467	PF00069	0.490
MOD_PKA_2	2	8	PF00069	0.649
MOD_PKA_2	259	265	PF00069	0.465
MOD_PKA_2	276	282	PF00069	0.210
MOD_PKA_2	374	380	PF00069	0.450
MOD_PKA_2	470	476	PF00069	0.683
MOD_PKA_2	49	55	PF00069	0.524
MOD_PKA_2	69	75	PF00069	0.653
MOD_Plk_4	122	128	PF00069	0.393
MOD_Plk_4	363	369	PF00069	0.450
MOD_Plk_4	94	100	PF00069	0.314
MOD_ProDKin_1	14	20	PF00069	0.578
MOD_ProDKin_1	230	236	PF00069	0.593
MOD_ProDKin_1	485	491	PF00069	0.735
MOD_SUMO_rev_2	118	126	PF00179	0.426
MOD_SUMO_rev_2	210	216	PF00179	0.304
MOD_SUMO_rev_2	463	470	PF00179	0.614
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.372
TRG_ENDOCYTIC_2	28	31	PF00928	0.419
TRG_ER_diArg_1	133	135	PF00400	0.300
TRG_ER_diArg_1	324	326	PF00400	0.325
TRG_ER_diArg_1	329	331	PF00400	0.315
TRG_ER_diArg_1	373	376	PF00400	0.444
TRG_Pf-PMV_PEXEL_1	144	148	PF00026	0.435
TRG_Pf-PMV_PEXEL_1	478	482	PF00026	0.649

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL90	Leptomonas seymouri	59%	98%
A0A0S4IRI2	Bodo saltans	37%	95%
A0A1X0NR87	Trypanosomatidae	44%	100%
A0A3S7X1P4	Leishmania donovani	94%	100%
A0A422N7D8	Trypanosoma rangeli	42%	100%
A4HGR8	Leishmania braziliensis	81%	100%
A4I3U3	Leishmania infantum	94%	100%
D0A913	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9B035	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
F4JRF4	Arabidopsis thaliana	34%	80%
O31584	Bacillus subtilis (strain 168)	31%	100%
P17802	Escherichia coli (strain K12)	31%	100%
P44320	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	29%	100%
P57617	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	25%	100%
P83847	Geobacillus stearothermophilus	34%	100%
Q05869	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	29%	100%
Q10159	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
Q8R5G2	Rattus norvegicus	30%	97%
Q99P21	Mus musculus	31%	97%
Q9UIF7	Homo sapiens	31%	92%
V5B3S1	Trypanosoma cruzi	41%	100%