Protein RFT1 homolog

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dolichyl-lipid chaperone, putative

Species:

Leishmania major

UniProt:

Q4Q823_LEIMA

TriTrypDb:

LmjF.28.2410 , LMJLV39_280032200 , LMJSD75_280031800

Length:

712

Annotations

LeishMANIAdb annotations

Highly conserved Eukaryotic protein. Involved in N-linked oligosaccharide assembly.. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	2
GO:0005783	endoplasmic reticulum	5	2
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0016020	membrane	2	11
GO:0031090	organelle membrane	3	2
GO:0031967	organelle envelope	3	2
GO:0031975	envelope	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

Q4Q823

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q823

Function

Biological processes
Term	Name	Level	Count
GO:0006488	dolichol-linked oligosaccharide biosynthetic process	5	11
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	11
GO:0006629	lipid metabolic process	3	11
GO:0006810	transport	3	2
GO:0006869	lipid transport	5	2
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	2
GO:0034203	glycolipid translocation	5	2
GO:0034204	lipid translocation	4	2
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044255	cellular lipid metabolic process	3	11
GO:0046836	glycolipid transport	6	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0061024	membrane organization	4	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0071702	organic substance transport	4	2
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	2
GO:0097035	regulation of membrane lipid distribution	3	2
GO:1901135	carbohydrate derivative metabolic process	3	11
GO:1901137	carbohydrate derivative biosynthetic process	4	11
GO:1901264	carbohydrate derivative transport	5	2
GO:1901576	organic substance biosynthetic process	3	11

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	180	184	PF00656	0.535
CLV_C14_Caspase3-7	341	345	PF00656	0.502
CLV_C14_Caspase3-7	373	377	PF00656	0.567
CLV_NRD_NRD_1	173	175	PF00675	0.674
CLV_NRD_NRD_1	382	384	PF00675	0.386
CLV_PCSK_KEX2_1	382	384	PF00082	0.397
CLV_PCSK_SKI1_1	24	28	PF00082	0.355
CLV_PCSK_SKI1_1	277	281	PF00082	0.553
CLV_PCSK_SKI1_1	36	40	PF00082	0.325
CLV_PCSK_SKI1_1	361	365	PF00082	0.359
CLV_PCSK_SKI1_1	398	402	PF00082	0.289
CLV_PCSK_SKI1_1	433	437	PF00082	0.282
CLV_PCSK_SKI1_1	464	468	PF00082	0.386
CLV_PCSK_SKI1_1	486	490	PF00082	0.528
CLV_PCSK_SKI1_1	496	500	PF00082	0.490
CLV_PCSK_SKI1_1	61	65	PF00082	0.392
CLV_PCSK_SKI1_1	644	648	PF00082	0.311
CLV_PCSK_SKI1_1	687	691	PF00082	0.619
CLV_PCSK_SKI1_1	699	703	PF00082	0.653
DEG_APCC_DBOX_1	392	400	PF00400	0.562
DEG_MDM2_SWIB_1	579	587	PF02201	0.480
DEG_ODPH_VHL_1	4	17	PF01847	0.455
DEG_SCF_FBW7_1	689	696	PF00400	0.484
DEG_SPOP_SBC_1	605	609	PF00917	0.586
DOC_CDC14_PxL_1	188	196	PF14671	0.442
DOC_CKS1_1	497	502	PF01111	0.362
DOC_CKS1_1	690	695	PF01111	0.462
DOC_CYCLIN_RxL_1	430	440	PF00134	0.562
DOC_CYCLIN_yCln2_LP_2	194	200	PF00134	0.369
DOC_CYCLIN_yCln2_LP_2	353	359	PF00134	0.530
DOC_MAPK_FxFP_2	365	368	PF00069	0.562
DOC_MAPK_gen_1	658	668	PF00069	0.585
DOC_MAPK_MEF2A_6	10	19	PF00069	0.354
DOC_MAPK_MEF2A_6	126	133	PF00069	0.562
DOC_MAPK_MEF2A_6	393	401	PF00069	0.513
DOC_MAPK_MEF2A_6	464	471	PF00069	0.543
DOC_MAPK_MEF2A_6	584	593	PF00069	0.355
DOC_MAPK_NFAT4_5	10	18	PF00069	0.362
DOC_MAPK_NFAT4_5	464	472	PF00069	0.586
DOC_PP1_RVXF_1	22	29	PF00149	0.346
DOC_PP1_RVXF_1	431	437	PF00149	0.482
DOC_PP1_RVXF_1	466	472	PF00149	0.408
DOC_PP2B_LxvP_1	194	197	PF13499	0.443
DOC_PP2B_LxvP_1	353	356	PF13499	0.530
DOC_PP2B_LxvP_1	37	40	PF13499	0.609
DOC_PP2B_LxvP_1	480	483	PF13499	0.357
DOC_PP2B_LxvP_1	626	629	PF13499	0.687
DOC_PP2B_LxvP_1	703	706	PF13499	0.360
DOC_PP4_FxxP_1	365	368	PF00568	0.562
DOC_USP7_MATH_1	108	112	PF00917	0.803
DOC_USP7_MATH_1	127	131	PF00917	0.396
DOC_USP7_MATH_1	132	136	PF00917	0.361
DOC_USP7_MATH_1	166	170	PF00917	0.461
DOC_USP7_MATH_1	338	342	PF00917	0.585
DOC_USP7_MATH_1	347	351	PF00917	0.474
DOC_USP7_MATH_1	500	504	PF00917	0.386
DOC_USP7_MATH_1	606	610	PF00917	0.746
DOC_USP7_MATH_1	622	626	PF00917	0.747
DOC_USP7_MATH_1	80	84	PF00917	0.753
DOC_WW_Pin1_4	112	117	PF00397	0.740
DOC_WW_Pin1_4	258	263	PF00397	0.391
DOC_WW_Pin1_4	496	501	PF00397	0.313
DOC_WW_Pin1_4	528	533	PF00397	0.562
DOC_WW_Pin1_4	592	597	PF00397	0.569
DOC_WW_Pin1_4	627	632	PF00397	0.692
DOC_WW_Pin1_4	689	694	PF00397	0.457
DOC_WW_Pin1_4	94	99	PF00397	0.797
LIG_14-3-3_CanoR_1	114	120	PF00244	0.716
LIG_14-3-3_CanoR_1	167	173	PF00244	0.492
LIG_14-3-3_CanoR_1	254	262	PF00244	0.337
LIG_14-3-3_CanoR_1	361	366	PF00244	0.539
LIG_14-3-3_CanoR_1	398	407	PF00244	0.511
LIG_14-3-3_CanoR_1	456	463	PF00244	0.524
LIG_Actin_WH2_2	385	400	PF00022	0.534
LIG_APCC_ABBA_1	49	54	PF00400	0.609
LIG_BRCT_BRCA1_1	129	133	PF00533	0.363
LIG_BRCT_BRCA1_1	473	477	PF00533	0.405
LIG_eIF4E_1	52	58	PF01652	0.522
LIG_FHA_1	201	207	PF00498	0.479
LIG_FHA_1	25	31	PF00498	0.330
LIG_FHA_1	288	294	PF00498	0.415
LIG_FHA_1	33	39	PF00498	0.360
LIG_FHA_1	46	52	PF00498	0.478
LIG_FHA_1	497	503	PF00498	0.349
LIG_FHA_1	62	68	PF00498	0.572
LIG_FHA_2	270	276	PF00498	0.356
LIG_FHA_2	310	316	PF00498	0.530
LIG_GBD_Chelix_1	15	23	PF00786	0.447
LIG_LIR_Apic_2	364	368	PF02991	0.563
LIG_LIR_Apic_2	494	500	PF02991	0.313
LIG_LIR_Gen_1	130	141	PF02991	0.363
LIG_LIR_Gen_1	474	485	PF02991	0.433
LIG_LIR_Gen_1	588	598	PF02991	0.475
LIG_LIR_Gen_1	637	646	PF02991	0.591
LIG_LIR_Gen_1	692	703	PF02991	0.498
LIG_LIR_Nem_3	130	136	PF02991	0.363
LIG_LIR_Nem_3	474	480	PF02991	0.406
LIG_LIR_Nem_3	48	52	PF02991	0.531
LIG_LIR_Nem_3	588	593	PF02991	0.410
LIG_LIR_Nem_3	637	641	PF02991	0.591
LIG_MYND_1	624	628	PF01753	0.703
LIG_NRBOX	33	39	PF00104	0.412
LIG_PCNA_yPIPBox_3	519	527	PF02747	0.408
LIG_PDZ_Class_1	707	712	PF00595	0.391
LIG_Pex14_2	365	369	PF04695	0.562
LIG_Pex14_2	471	475	PF04695	0.447
LIG_Pex14_2	579	583	PF04695	0.480
LIG_Pex14_2	638	642	PF04695	0.579
LIG_PTAP_UEV_1	104	109	PF05743	0.798
LIG_PTB_Apo_2	40	47	PF02174	0.564
LIG_PTB_Phospho_1	40	46	PF10480	0.564
LIG_SH2_CRK	295	299	PF00017	0.441
LIG_SH2_CRK	384	388	PF00017	0.571
LIG_SH2_CRK	421	425	PF00017	0.482
LIG_SH2_CRK	511	515	PF00017	0.341
LIG_SH2_CRK	675	679	PF00017	0.332
LIG_SH2_GRB2like	41	44	PF00017	0.541
LIG_SH2_NCK_1	372	376	PF00017	0.530
LIG_SH2_PTP2	554	557	PF00017	0.378
LIG_SH2_SRC	41	44	PF00017	0.586
LIG_SH2_STAP1	295	299	PF00017	0.441
LIG_SH2_STAP1	384	388	PF00017	0.534
LIG_SH2_STAP1	493	497	PF00017	0.356
LIG_SH2_STAP1	675	679	PF00017	0.480
LIG_SH2_STAT3	250	253	PF00017	0.362
LIG_SH2_STAT5	46	49	PF00017	0.520
LIG_SH2_STAT5	472	475	PF00017	0.324
LIG_SH2_STAT5	493	496	PF00017	0.339
LIG_SH2_STAT5	509	512	PF00017	0.224
LIG_SH2_STAT5	549	552	PF00017	0.342
LIG_SH2_STAT5	554	557	PF00017	0.328
LIG_SH2_STAT5	590	593	PF00017	0.495
LIG_SH3_3	102	108	PF00018	0.787
LIG_SH3_3	194	200	PF00018	0.400
LIG_SH3_3	526	532	PF00018	0.586
LIG_SH3_3	590	596	PF00018	0.445
LIG_SH3_3	615	621	PF00018	0.772
LIG_SH3_3	625	631	PF00018	0.602
LIG_Sin3_3	319	326	PF02671	0.586
LIG_SUMO_SIM_anti_2	568	574	PF11976	0.364
LIG_SUMO_SIM_par_1	217	223	PF11976	0.391
LIG_SUMO_SIM_par_1	228	234	PF11976	0.513
LIG_SUMO_SIM_par_1	664	669	PF11976	0.246
LIG_TRFH_1	193	197	PF08558	0.493
LIG_TYR_ITIM	292	297	PF00017	0.447
LIG_TYR_ITIM	673	678	PF00017	0.402
LIG_UBA3_1	17	24	PF00899	0.402
LIG_Vh1_VBS_1	138	156	PF01044	0.440
LIG_WRC_WIRS_1	155	160	PF05994	0.495
LIG_WRC_WIRS_1	25	30	PF05994	0.354
LIG_WRC_WIRS_1	388	393	PF05994	0.480
LIG_WRC_WIRS_1	46	51	PF05994	0.504
LIG_WRC_WIRS_1	472	477	PF05994	0.484
MOD_CDK_SPxxK_3	94	101	PF00069	0.774
MOD_CK1_1	106	112	PF00069	0.727
MOD_CK1_1	186	192	PF00069	0.594
MOD_CK1_1	29	35	PF00069	0.447
MOD_CK1_1	411	417	PF00069	0.415
MOD_CK1_1	426	432	PF00069	0.264
MOD_CK1_1	664	670	PF00069	0.405
MOD_CK2_1	269	275	PF00069	0.440
MOD_CK2_1	309	315	PF00069	0.448
MOD_CK2_1	399	405	PF00069	0.355
MOD_CK2_1	562	568	PF00069	0.439
MOD_CK2_1	94	100	PF00069	0.768
MOD_GlcNHglycan	105	108	PF01048	0.762
MOD_GlcNHglycan	111	114	PF01048	0.720
MOD_GlcNHglycan	179	182	PF01048	0.600
MOD_GlcNHglycan	255	258	PF01048	0.416
MOD_GlcNHglycan	299	302	PF01048	0.447
MOD_GlcNHglycan	340	343	PF01048	0.427
MOD_GlcNHglycan	350	353	PF01048	0.353
MOD_GlcNHglycan	608	611	PF01048	0.713
MOD_GlcNHglycan	75	79	PF01048	0.626
MOD_GlcNHglycan	81	85	PF01048	0.697
MOD_GSK3_1	108	115	PF00069	0.772
MOD_GSK3_1	154	161	PF00069	0.495
MOD_GSK3_1	24	31	PF00069	0.335
MOD_GSK3_1	293	300	PF00069	0.446
MOD_GSK3_1	329	336	PF00069	0.460
MOD_GSK3_1	370	377	PF00069	0.425
MOD_GSK3_1	399	406	PF00069	0.447
MOD_GSK3_1	407	414	PF00069	0.414
MOD_GSK3_1	422	429	PF00069	0.285
MOD_GSK3_1	445	452	PF00069	0.432
MOD_GSK3_1	471	478	PF00069	0.453
MOD_GSK3_1	484	491	PF00069	0.338
MOD_GSK3_1	492	499	PF00069	0.354
MOD_GSK3_1	562	569	PF00069	0.473
MOD_GSK3_1	601	608	PF00069	0.677
MOD_GSK3_1	689	696	PF00069	0.594
MOD_N-GLC_1	177	182	PF02516	0.602
MOD_N-GLC_2	441	443	PF02516	0.378
MOD_NEK2_1	17	22	PF00069	0.408
MOD_NEK2_1	220	225	PF00069	0.490
MOD_NEK2_1	26	31	PF00069	0.250
MOD_NEK2_1	297	302	PF00069	0.413
MOD_NEK2_1	308	313	PF00069	0.391
MOD_NEK2_1	387	392	PF00069	0.360
MOD_NEK2_1	407	412	PF00069	0.150
MOD_NEK2_1	423	428	PF00069	0.319
MOD_NEK2_1	471	476	PF00069	0.362
MOD_NEK2_1	484	489	PF00069	0.388
MOD_NEK2_1	492	497	PF00069	0.338
MOD_NEK2_1	591	596	PF00069	0.537
MOD_NEK2_1	654	659	PF00069	0.424
MOD_NEK2_1	707	712	PF00069	0.686
MOD_NEK2_2	585	590	PF00069	0.447
MOD_PIKK_1	166	172	PF00454	0.635
MOD_PIKK_1	562	568	PF00454	0.402
MOD_PK_1	661	667	PF00069	0.480
MOD_PKA_2	166	172	PF00069	0.574
MOD_PKA_2	253	259	PF00069	0.477
MOD_PKA_2	329	335	PF00069	0.246
MOD_PKA_2	392	398	PF00069	0.378
MOD_PKA_2	455	461	PF00069	0.434
MOD_PKB_1	101	109	PF00069	0.673
MOD_Plk_1	200	206	PF00069	0.428
MOD_Plk_1	449	455	PF00069	0.422
MOD_Plk_1	661	667	PF00069	0.246
MOD_Plk_4	115	121	PF00069	0.596
MOD_Plk_4	154	160	PF00069	0.550
MOD_Plk_4	200	206	PF00069	0.368
MOD_Plk_4	234	240	PF00069	0.428
MOD_Plk_4	241	247	PF00069	0.358
MOD_Plk_4	29	35	PF00069	0.355
MOD_Plk_4	293	299	PF00069	0.417
MOD_Plk_4	370	376	PF00069	0.435
MOD_Plk_4	387	393	PF00069	0.390
MOD_Plk_4	426	432	PF00069	0.369
MOD_Plk_4	484	490	PF00069	0.462
MOD_Plk_4	543	549	PF00069	0.394
MOD_Plk_4	553	559	PF00069	0.426
MOD_Plk_4	568	574	PF00069	0.440
MOD_Plk_4	578	584	PF00069	0.398
MOD_Plk_4	585	591	PF00069	0.298
MOD_Plk_4	637	643	PF00069	0.490
MOD_Plk_4	646	652	PF00069	0.424
MOD_Plk_4	661	667	PF00069	0.395
MOD_ProDKin_1	112	118	PF00069	0.681
MOD_ProDKin_1	258	264	PF00069	0.488
MOD_ProDKin_1	496	502	PF00069	0.378
MOD_ProDKin_1	528	534	PF00069	0.447
MOD_ProDKin_1	592	598	PF00069	0.463
MOD_ProDKin_1	627	633	PF00069	0.613
MOD_ProDKin_1	689	695	PF00069	0.584
MOD_ProDKin_1	94	100	PF00069	0.774
MOD_SUMO_rev_2	341	348	PF00179	0.363
TRG_DiLeu_BaEn_1	234	239	PF01217	0.355
TRG_DiLeu_BaEn_1	685	690	PF01217	0.550
TRG_DiLeu_BaEn_2	439	445	PF01217	0.378
TRG_DiLeu_BaLyEn_6	33	38	PF01217	0.526
TRG_DiLeu_BaLyEn_6	430	435	PF01217	0.408
TRG_DiLeu_BaLyEn_6	697	702	PF01217	0.641
TRG_ENDOCYTIC_2	294	297	PF00928	0.447
TRG_ENDOCYTIC_2	372	375	PF00928	0.403
TRG_ENDOCYTIC_2	384	387	PF00928	0.356
TRG_ENDOCYTIC_2	420	423	PF00928	0.339
TRG_ENDOCYTIC_2	46	49	PF00928	0.486
TRG_ENDOCYTIC_2	472	475	PF00928	0.447
TRG_ENDOCYTIC_2	554	557	PF00928	0.360
TRG_ENDOCYTIC_2	590	593	PF00928	0.495
TRG_ENDOCYTIC_2	675	678	PF00928	0.415
TRG_ER_diArg_1	382	384	PF00400	0.402
TRG_ER_diArg_1	697	700	PF00400	0.525
TRG_NES_CRM1_1	201	215	PF08389	0.307
TRG_NES_CRM1_1	222	234	PF08389	0.447
TRG_Pf-PMV_PEXEL_1	658	662	PF00026	0.376

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHL6	Leptomonas seymouri	60%	99%
A0A1X0NQN5	Trypanosomatidae	34%	100%
A0A3Q8IDZ2	Leishmania donovani	96%	98%
A0A3S5IQL5	Trypanosoma rangeli	33%	100%
A4HGU4	Leishmania braziliensis	79%	98%
A4I3X2	Leishmania infantum	95%	98%
D0A939	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9B062	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	98%
V5AWN8	Trypanosoma cruzi	34%	100%