Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase, putative

Species:

Leishmania major

UniProt:

Q4Q822_LEIMA

TriTrypDb:

LmjF.28.2420 , LMJLV39_280032300 , LMJSD75_280031900

Length:

389

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0005815	microtubule organizing center	2	2
GO:0036064	ciliary basal body	3	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:0032991	protein-containing complex	1	2
GO:0045239	tricarboxylic acid cycle enzyme complex	3	2
GO:0045240	dihydrolipoyl dehydrogenase complex	4	2
GO:0045252	oxoglutarate dehydrogenase complex	4	2
GO:1902494	catalytic complex	2	2
GO:1990204	oxidoreductase complex	3	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

Q4Q822

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q822

Function

Biological processes
Term	Name	Level	Count
GO:0006099	tricarboxylic acid cycle	3	4
GO:0008152	metabolic process	1	4
GO:0044238	primary metabolic process	2	4
GO:0006082	organic acid metabolic process	3	2
GO:0006084	acetyl-CoA metabolic process	5	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006163	purine nucleotide metabolic process	5	2
GO:0006520	amino acid metabolic process	3	2
GO:0006553	lysine metabolic process	6	2
GO:0006554	lysine catabolic process	5	2
GO:0006637	acyl-CoA metabolic process	4	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006753	nucleoside phosphate metabolic process	4	2
GO:0006790	sulfur compound metabolic process	3	2
GO:0006793	phosphorus metabolic process	3	2
GO:0006796	phosphate-containing compound metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0009056	catabolic process	2	2
GO:0009063	amino acid catabolic process	4	2
GO:0009066	aspartate family amino acid metabolic process	5	2
GO:0009068	aspartate family amino acid catabolic process	6	2
GO:0009117	nucleotide metabolic process	5	2
GO:0009150	purine ribonucleotide metabolic process	6	2
GO:0009259	ribonucleotide metabolic process	5	2
GO:0009987	cellular process	1	2
GO:0016054	organic acid catabolic process	4	2
GO:0019474	L-lysine catabolic process to acetyl-CoA	4	2
GO:0019477	L-lysine catabolic process	6	2
GO:0019637	organophosphate metabolic process	3	2
GO:0019693	ribose phosphate metabolic process	4	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0033512	L-lysine catabolic process to acetyl-CoA via saccharopine	5	2
GO:0033865	nucleoside bisphosphate metabolic process	5	2
GO:0033875	ribonucleoside bisphosphate metabolic process	6	2
GO:0034032	purine nucleoside bisphosphate metabolic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0035383	thioester metabolic process	3	2
GO:0043436	oxoacid metabolic process	4	2
GO:0043603	amide metabolic process	3	2
GO:0043605	amide catabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044281	small molecule metabolic process	2	2
GO:0044282	small molecule catabolic process	3	2
GO:0046395	carboxylic acid catabolic process	5	2
GO:0046440	L-lysine metabolic process	7	2
GO:0046483	heterocycle metabolic process	3	2
GO:0055086	nucleobase-containing small molecule metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0072521	purine-containing compound metabolic process	4	2
GO:1901135	carbohydrate derivative metabolic process	3	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901565	organonitrogen compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2
GO:1901605	alpha-amino acid metabolic process	4	2
GO:1901606	alpha-amino acid catabolic process	5	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004149	dihydrolipoyllysine-residue succinyltransferase activity	3	4
GO:0016417	S-acyltransferase activity	5	4
GO:0016740	transferase activity	2	11
GO:0016746	acyltransferase activity	3	11
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	4
GO:0016748	succinyltransferase activity	5	4
GO:0016751	S-succinyltransferase activity	6	4
GO:0140096	catalytic activity, acting on a protein	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	363	367	PF00656	0.376
CLV_NRD_NRD_1	169	171	PF00675	0.521
CLV_NRD_NRD_1	3	5	PF00675	0.627
CLV_PCSK_KEX2_1	168	170	PF00082	0.521
CLV_PCSK_KEX2_1	209	211	PF00082	0.449
CLV_PCSK_KEX2_1	3	5	PF00082	0.627
CLV_PCSK_PC1ET2_1	209	211	PF00082	0.449
CLV_PCSK_SKI1_1	383	387	PF00082	0.375
DEG_Nend_Nbox_1	1	3	PF02207	0.632
DOC_CKS1_1	256	261	PF01111	0.376
DOC_CKS1_1	316	321	PF01111	0.376
DOC_MAPK_gen_1	209	217	PF00069	0.376
DOC_MAPK_gen_1	346	355	PF00069	0.419
DOC_MAPK_MEF2A_6	209	217	PF00069	0.376
DOC_MAPK_MEF2A_6	23	30	PF00069	0.550
DOC_MAPK_MEF2A_6	346	355	PF00069	0.382
DOC_PP1_RVXF_1	39	46	PF00149	0.376
DOC_PP1_RVXF_1	79	85	PF00149	0.387
DOC_PP2B_LxvP_1	117	120	PF13499	0.743
DOC_PP2B_LxvP_1	262	265	PF13499	0.366
DOC_USP7_MATH_1	130	134	PF00917	0.747
DOC_WW_Pin1_4	191	196	PF00397	0.376
DOC_WW_Pin1_4	216	221	PF00397	0.376
DOC_WW_Pin1_4	255	260	PF00397	0.376
DOC_WW_Pin1_4	315	320	PF00397	0.376
LIG_14-3-3_CanoR_1	158	163	PF00244	0.392
LIG_14-3-3_CanoR_1	174	184	PF00244	0.341
LIG_14-3-3_CanoR_1	3	11	PF00244	0.667
LIG_Actin_WH2_2	160	178	PF00022	0.488
LIG_FHA_1	157	163	PF00498	0.490
LIG_FHA_1	301	307	PF00498	0.376
LIG_FHA_1	316	322	PF00498	0.376
LIG_FHA_1	367	373	PF00498	0.388
LIG_FHA_1	37	43	PF00498	0.389
LIG_FHA_1	44	50	PF00498	0.359
LIG_FHA_1	5	11	PF00498	0.736
LIG_FHA_2	185	191	PF00498	0.371
LIG_FHA_2	293	299	PF00498	0.385
LIG_FHA_2	30	36	PF00498	0.376
LIG_FHA_2	96	102	PF00498	0.584
LIG_LIR_Gen_1	186	196	PF02991	0.376
LIG_LIR_Gen_1	243	254	PF02991	0.385
LIG_LIR_Nem_3	186	191	PF02991	0.372
LIG_LIR_Nem_3	243	249	PF02991	0.382
LIG_LRP6_Inhibitor_1	340	346	PF00058	0.419
LIG_PTB_Apo_2	233	240	PF02174	0.396
LIG_SH2_STAP1	285	289	PF00017	0.488
LIG_SH2_STAP1	357	361	PF00017	0.376
LIG_SH3_3	132	138	PF00018	0.728
LIG_SH3_3	253	259	PF00018	0.376
LIG_SH3_3	68	74	PF00018	0.376
LIG_SUMO_SIM_par_1	384	389	PF11976	0.649
LIG_UBA3_1	196	204	PF00899	0.376
LIG_UBA3_1	328	336	PF00899	0.360
LIG_WRC_WIRS_1	185	190	PF05994	0.376
MOD_CDK_SPK_2	255	260	PF00069	0.376
MOD_CDK_SPxxK_3	216	223	PF00069	0.376
MOD_CK1_1	178	184	PF00069	0.376
MOD_CK1_1	36	42	PF00069	0.376
MOD_CK2_1	184	190	PF00069	0.371
MOD_CK2_1	29	35	PF00069	0.376
MOD_CK2_1	292	298	PF00069	0.396
MOD_CK2_1	95	101	PF00069	0.581
MOD_GlcNHglycan	132	135	PF01048	0.763
MOD_GlcNHglycan	14	17	PF01048	0.735
MOD_GlcNHglycan	224	227	PF01048	0.495
MOD_GlcNHglycan	325	328	PF01048	0.376
MOD_GSK3_1	158	165	PF00069	0.433
MOD_GSK3_1	29	36	PF00069	0.382
MOD_GSK3_1	300	307	PF00069	0.376
MOD_GSK3_1	311	318	PF00069	0.376
MOD_GSK3_1	362	369	PF00069	0.419
MOD_N-GLC_1	235	240	PF02516	0.376
MOD_N-GLC_1	43	48	PF02516	0.417
MOD_N-GLC_2	180	182	PF02516	0.396
MOD_NEK2_1	175	180	PF00069	0.376
MOD_NEK2_1	184	189	PF00069	0.366
MOD_NEK2_1	24	29	PF00069	0.535
MOD_NEK2_1	304	309	PF00069	0.376
MOD_PIKK_1	175	181	PF00454	0.376
MOD_PK_1	158	164	PF00069	0.419
MOD_PKA_1	3	9	PF00069	0.667
MOD_PKA_2	162	168	PF00069	0.376
MOD_PKA_2	3	9	PF00069	0.667
MOD_Plk_1	235	241	PF00069	0.376
MOD_Plk_4	311	317	PF00069	0.376
MOD_Plk_4	366	372	PF00069	0.396
MOD_ProDKin_1	191	197	PF00069	0.376
MOD_ProDKin_1	216	222	PF00069	0.376
MOD_ProDKin_1	255	261	PF00069	0.376
MOD_ProDKin_1	315	321	PF00069	0.376
MOD_SUMO_for_1	120	123	PF00179	0.763
MOD_SUMO_rev_2	206	215	PF00179	0.475
TRG_DiLeu_BaLyEn_6	357	362	PF01217	0.376
TRG_ENDOCYTIC_2	246	249	PF00928	0.379
TRG_ER_diArg_1	167	170	PF00400	0.407
TRG_ER_diArg_1	2	4	PF00400	0.633
TRG_ER_diArg_1	288	291	PF00400	0.488
TRG_Pf-PMV_PEXEL_1	169	173	PF00026	0.521
TRG_Pf-PMV_PEXEL_1	375	379	PF00026	0.376

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HSX4	Leptomonas seymouri	85%	100%
A0A0S4IY01	Bodo saltans	70%	100%
A0A0S4JS90	Bodo saltans	27%	86%
A0A1X0NQZ2	Trypanosomatidae	72%	100%
A0A1X0P2L7	Trypanosomatidae	27%	78%
A0A3Q8IGX8	Leishmania donovani	97%	100%
A0A3R7MEC2	Trypanosoma rangeli	70%	99%
A4HGU5	Leishmania braziliensis	89%	99%
A4HPD2	Leishmania braziliensis	32%	84%
A4I3X3	Leishmania infantum	97%	100%
C9ZPW7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	89%
D0A3J2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	86%
D0A940	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	74%	100%
E9AG63	Leishmania infantum	33%	82%
E9B063	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
G0S4X6	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	29%	85%
O06159	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	30%	99%
O31550	Bacillus subtilis (strain 168)	30%	98%
O94681	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	42%	86%
P09062	Pseudomonas putida	30%	92%
P0AFG6	Escherichia coli (strain K12)	40%	96%
P0AFG7	Escherichia coli O157:H7	40%	96%
P11179	Bos taurus	51%	85%
P12695	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	81%
P16263	Bacillus subtilis (strain 168)	40%	93%
P19262	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	84%
P20285	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	28%	85%
P20708	Azotobacter vinelandii	44%	97%
P21883	Bacillus subtilis (strain 168)	36%	88%
P36957	Homo sapiens	47%	86%
P45302	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	41%	95%
P47514	Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)	28%	100%
P53395	Mus musculus	33%	81%
P65635	Staphylococcus aureus (strain Mu50 / ATCC 700699)	31%	90%
P65636	Staphylococcus aureus (strain N315)	31%	90%
P75392	Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1)	27%	97%
Q01205	Rattus norvegicus	48%	86%
Q19749	Caenorhabditis elegans	32%	77%
Q1RHI5	Rickettsia bellii (strain RML369-C)	43%	97%
Q1RJT3	Rickettsia bellii (strain RML369-C)	30%	93%
Q23571	Caenorhabditis elegans	26%	87%
Q2FH26	Staphylococcus aureus (strain USA300)	37%	92%
Q2FYM2	Staphylococcus aureus (strain NCTC 8325 / PS 47)	37%	92%
Q2YY06	Staphylococcus aureus (strain bovine RF122 / ET3-1)	37%	92%
Q49110	Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154)	33%	89%
Q49XM4	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	38%	92%
Q4L6C3	Staphylococcus haemolyticus (strain JCSC1435)	37%	92%
Q4UKI7	Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)	42%	97%
Q4ULG1	Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)	29%	94%
Q59821	Staphylococcus aureus	31%	90%
Q5HG07	Staphylococcus aureus (strain COL)	37%	92%
Q5HGY9	Staphylococcus aureus (strain COL)	31%	90%
Q5HPC7	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	38%	93%
Q5M729	Arabidopsis thaliana	29%	72%
Q68WK6	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	30%	96%
Q68XI8	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	42%	98%
Q6FYD4	Bartonella quintana (strain Toulouse)	43%	95%
Q6G9E9	Staphylococcus aureus (strain MSSA476)	37%	92%
Q6GAB9	Staphylococcus aureus (strain MSSA476)	31%	90%
Q6GGZ6	Staphylococcus aureus (strain MRSA252)	36%	92%
Q6GHZ0	Staphylococcus aureus (strain MRSA252)	31%	90%
Q7A5N4	Staphylococcus aureus (strain N315)	37%	92%
Q869Y7	Dictyostelium discoideum	51%	89%
Q8BKZ9	Mus musculus	26%	78%
Q8CSL9	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	38%	93%
Q8GCY1	Bartonella vinsonii subsp. berkhoffii	43%	95%
Q8H107	Arabidopsis thaliana	47%	84%
Q8K9N2	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	37%	99%
Q8K9T8	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	26%	97%
Q8NWR7	Staphylococcus aureus (strain MW2)	37%	92%
Q8NX76	Staphylococcus aureus (strain MW2)	31%	90%
Q8RWN9	Arabidopsis thaliana	27%	72%
Q90512	Takifugu rubripes	50%	95%
Q92HK7	Rickettsia conorii (strain ATCC VR-613 / Malish 7)	29%	94%
Q92J43	Rickettsia conorii (strain ATCC VR-613 / Malish 7)	43%	98%
Q99U75	Staphylococcus aureus (strain Mu50 / ATCC 700699)	37%	92%
Q9D2G2	Mus musculus	49%	86%
Q9FLQ4	Arabidopsis thaliana	47%	84%
Q9I3D2	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	43%	95%
Q9M7Z1	Arabidopsis thaliana	26%	81%
Q9N0F1	Sus scrofa	48%	85%
Q9ZD20	Rickettsia prowazekii (strain Madrid E)	29%	95%
Q9ZDY4	Rickettsia prowazekii (strain Madrid E)	41%	97%
V5BFP9	Trypanosoma cruzi	31%	83%