ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair and recombination helicase protein PIF3, putative

Species:

Leishmania major

UniProt:

Q4Q810_LEIMA

TriTrypDb:

LmjF.28.2530 , LMJLV39_280033500 , LMJSD75_280033200

Length:

936

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005657	replication fork	2	2
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

Q4Q810

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q810

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006260	DNA replication	5	2
GO:0006281	DNA repair	5	11
GO:0006310	DNA recombination	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0032200	telomere organization	6	11
GO:0032392	DNA geometric change	7	2
GO:0032508	DNA duplex unwinding	8	2
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051276	chromosome organization	5	11
GO:0051716	cellular response to stimulus	2	11
GO:0071103	DNA conformation change	6	2
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0000287	magnesium ion binding	5	2
GO:0003678	DNA helicase activity	3	11
GO:0003824	catalytic activity	1	11
GO:0004386	helicase activity	2	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008094	ATP-dependent activity, acting on DNA	2	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0016887	ATP hydrolysis activity	7	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	378	382	PF00656	0.327
CLV_C14_Caspase3-7	383	387	PF00656	0.330
CLV_C14_Caspase3-7	621	625	PF00656	0.789
CLV_C14_Caspase3-7	71	75	PF00656	0.633
CLV_C14_Caspase3-7	723	727	PF00656	0.466
CLV_NRD_NRD_1	157	159	PF00675	0.665
CLV_NRD_NRD_1	227	229	PF00675	0.524
CLV_NRD_NRD_1	458	460	PF00675	0.442
CLV_NRD_NRD_1	488	490	PF00675	0.453
CLV_NRD_NRD_1	563	565	PF00675	0.733
CLV_NRD_NRD_1	660	662	PF00675	0.752
CLV_NRD_NRD_1	673	675	PF00675	0.622
CLV_NRD_NRD_1	773	775	PF00675	0.532
CLV_NRD_NRD_1	874	876	PF00675	0.527
CLV_NRD_NRD_1	893	895	PF00675	0.489
CLV_PCSK_FUR_1	671	675	PF00082	0.721
CLV_PCSK_KEX2_1	156	158	PF00082	0.688
CLV_PCSK_KEX2_1	227	229	PF00082	0.566
CLV_PCSK_KEX2_1	315	317	PF00082	0.274
CLV_PCSK_KEX2_1	458	460	PF00082	0.503
CLV_PCSK_KEX2_1	487	489	PF00082	0.474
CLV_PCSK_KEX2_1	670	672	PF00082	0.692
CLV_PCSK_KEX2_1	673	675	PF00082	0.631
CLV_PCSK_KEX2_1	773	775	PF00082	0.532
CLV_PCSK_KEX2_1	784	786	PF00082	0.421
CLV_PCSK_KEX2_1	893	895	PF00082	0.486
CLV_PCSK_PC1ET2_1	315	317	PF00082	0.252
CLV_PCSK_PC1ET2_1	670	672	PF00082	0.741
CLV_PCSK_PC1ET2_1	784	786	PF00082	0.366
CLV_PCSK_PC7_1	152	158	PF00082	0.622
CLV_PCSK_PC7_1	223	229	PF00082	0.574
CLV_PCSK_PC7_1	780	786	PF00082	0.377
CLV_PCSK_SKI1_1	115	119	PF00082	0.418
CLV_PCSK_SKI1_1	167	171	PF00082	0.662
CLV_PCSK_SKI1_1	223	227	PF00082	0.596
CLV_PCSK_SKI1_1	236	240	PF00082	0.489
CLV_PCSK_SKI1_1	292	296	PF00082	0.342
CLV_PCSK_SKI1_1	310	314	PF00082	0.311
CLV_PCSK_SKI1_1	437	441	PF00082	0.354
CLV_PCSK_SKI1_1	524	528	PF00082	0.458
CLV_PCSK_SKI1_1	595	599	PF00082	0.488
CLV_PCSK_SKI1_1	673	677	PF00082	0.642
CLV_PCSK_SKI1_1	698	702	PF00082	0.517
CLV_PCSK_SKI1_1	780	784	PF00082	0.558
CLV_PCSK_SKI1_1	894	898	PF00082	0.487
DEG_APCC_DBOX_1	523	531	PF00400	0.415
DEG_APCC_DBOX_1	543	551	PF00400	0.522
DEG_Nend_UBRbox_2	1	3	PF02207	0.679
DEG_SPOP_SBC_1	107	111	PF00917	0.539
DEG_SPOP_SBC_1	66	70	PF00917	0.680
DOC_ANK_TNKS_1	612	619	PF00023	0.594
DOC_CKS1_1	270	275	PF01111	0.634
DOC_CKS1_1	438	443	PF01111	0.408
DOC_CKS1_1	508	513	PF01111	0.494
DOC_CKS1_1	58	63	PF01111	0.551
DOC_CYCLIN_yCln2_LP_2	270	276	PF00134	0.631
DOC_CYCLIN_yCln2_LP_2	438	444	PF00134	0.408
DOC_CYCLIN_yCln2_LP_2	470	476	PF00134	0.603
DOC_CYCLIN_yCln2_LP_2	660	666	PF00134	0.525
DOC_CYCLIN_yCln2_LP_2	829	835	PF00134	0.516
DOC_MAPK_DCC_7	790	800	PF00069	0.509
DOC_MAPK_gen_1	661	667	PF00069	0.684
DOC_MAPK_gen_1	735	744	PF00069	0.385
DOC_MAPK_gen_1	773	783	PF00069	0.574
DOC_MAPK_gen_1	873	882	PF00069	0.389
DOC_MAPK_gen_1	893	899	PF00069	0.219
DOC_MAPK_MEF2A_6	431	438	PF00069	0.336
DOC_MAPK_RevD_3	880	894	PF00069	0.438
DOC_PP1_RVXF_1	778	784	PF00149	0.553
DOC_PP2B_LxvP_1	829	832	PF13499	0.518
DOC_PP4_FxxP_1	34	37	PF00568	0.616
DOC_PP4_FxxP_1	350	353	PF00568	0.335
DOC_PP4_FxxP_1	763	766	PF00568	0.418
DOC_PP4_FxxP_1	98	101	PF00568	0.409
DOC_SPAK_OSR1_1	461	465	PF12202	0.357
DOC_USP7_MATH_1	14	18	PF00917	0.692
DOC_USP7_MATH_1	189	193	PF00917	0.784
DOC_USP7_MATH_1	194	198	PF00917	0.747
DOC_USP7_MATH_1	424	428	PF00917	0.406
DOC_USP7_MATH_1	572	576	PF00917	0.654
DOC_USP7_MATH_1	65	69	PF00917	0.731
DOC_USP7_MATH_1	76	80	PF00917	0.766
DOC_USP7_MATH_1	766	770	PF00917	0.558
DOC_USP7_MATH_1	772	776	PF00917	0.597
DOC_USP7_MATH_1	906	910	PF00917	0.635
DOC_USP7_MATH_1	918	922	PF00917	0.536
DOC_WW_Pin1_4	132	137	PF00397	0.537
DOC_WW_Pin1_4	269	274	PF00397	0.573
DOC_WW_Pin1_4	397	402	PF00397	0.373
DOC_WW_Pin1_4	437	442	PF00397	0.398
DOC_WW_Pin1_4	507	512	PF00397	0.466
DOC_WW_Pin1_4	57	62	PF00397	0.732
DOC_WW_Pin1_4	729	734	PF00397	0.487
DOC_WW_Pin1_4	74	79	PF00397	0.546
DOC_WW_Pin1_4	911	916	PF00397	0.615
LIG_14-3-3_CanoR_1	108	113	PF00244	0.530
LIG_14-3-3_CanoR_1	193	202	PF00244	0.752
LIG_14-3-3_CanoR_1	327	336	PF00244	0.368
LIG_14-3-3_CanoR_1	44	48	PF00244	0.744
LIG_14-3-3_CanoR_1	673	678	PF00244	0.607
LIG_14-3-3_CanoR_1	698	703	PF00244	0.490
LIG_14-3-3_CanoR_1	773	781	PF00244	0.586
LIG_14-3-3_CanoR_1	893	899	PF00244	0.517
LIG_14-3-3_CanoR_1	920	924	PF00244	0.449
LIG_Actin_WH2_2	247	264	PF00022	0.538
LIG_Actin_WH2_2	314	329	PF00022	0.335
LIG_BIR_III_4	533	537	PF00653	0.673
LIG_BRCT_BRCA1_1	426	430	PF00533	0.335
LIG_BRCT_BRCA1_1	90	94	PF00533	0.605
LIG_EH_1	99	103	PF12763	0.427
LIG_FHA_1	107	113	PF00498	0.448
LIG_FHA_1	232	238	PF00498	0.553
LIG_FHA_1	280	286	PF00498	0.584
LIG_FHA_1	309	315	PF00498	0.329
LIG_FHA_1	331	337	PF00498	0.335
LIG_FHA_1	476	482	PF00498	0.570
LIG_FHA_1	592	598	PF00498	0.539
LIG_FHA_1	670	676	PF00498	0.706
LIG_FHA_1	828	834	PF00498	0.519
LIG_FHA_1	84	90	PF00498	0.654
LIG_FHA_1	850	856	PF00498	0.376
LIG_FHA_2	270	276	PF00498	0.546
LIG_FHA_2	69	75	PF00498	0.571
LIG_FHA_2	699	705	PF00498	0.547
LIG_FHA_2	716	722	PF00498	0.540
LIG_Integrin_isoDGR_2	103	105	PF01839	0.593
LIG_LIR_Apic_2	263	268	PF02991	0.459
LIG_LIR_Apic_2	33	37	PF02991	0.604
LIG_LIR_Apic_2	482	486	PF02991	0.425
LIG_LIR_Apic_2	55	61	PF02991	0.543
LIG_LIR_Apic_2	761	766	PF02991	0.417
LIG_LIR_Apic_2	96	101	PF02991	0.424
LIG_LIR_Gen_1	245	254	PF02991	0.547
LIG_LIR_Gen_1	412	420	PF02991	0.319
LIG_LIR_Gen_1	465	475	PF02991	0.514
LIG_LIR_LC3C_4	878	882	PF02991	0.490
LIG_LIR_Nem_3	400	405	PF02991	0.396
LIG_LIR_Nem_3	412	416	PF02991	0.310
LIG_LIR_Nem_3	427	433	PF02991	0.319
LIG_LIR_Nem_3	465	470	PF02991	0.509
LIG_LIR_Nem_3	683	687	PF02991	0.408
LIG_LIR_Nem_3	88	93	PF02991	0.631
LIG_PCNA_yPIPBox_3	251	262	PF02747	0.570
LIG_PCNA_yPIPBox_3	790	801	PF02747	0.522
LIG_PDZ_Class_2	931	936	PF00595	0.652
LIG_Pex14_2	118	122	PF04695	0.505
LIG_Pex14_2	346	350	PF04695	0.319
LIG_Pex14_2	94	98	PF04695	0.472
LIG_SH2_CRK	123	127	PF00017	0.558
LIG_SH2_CRK	258	262	PF00017	0.584
LIG_SH2_CRK	265	269	PF00017	0.628
LIG_SH2_CRK	58	62	PF00017	0.708
LIG_SH2_CRK	90	94	PF00017	0.616
LIG_SH2_GRB2like	249	252	PF00017	0.563
LIG_SH2_NCK_1	425	429	PF00017	0.440
LIG_SH2_NCK_1	58	62	PF00017	0.550
LIG_SH2_STAP1	425	429	PF00017	0.434
LIG_SH2_STAP1	837	841	PF00017	0.474
LIG_SH2_STAT3	883	886	PF00017	0.549
LIG_SH2_STAT5	483	486	PF00017	0.418
LIG_SH2_STAT5	822	825	PF00017	0.473
LIG_SH2_STAT5	868	871	PF00017	0.354
LIG_SH3_3	123	129	PF00018	0.503
LIG_SH3_3	18	24	PF00018	0.741
LIG_SH3_3	25	31	PF00018	0.761
LIG_SH3_3	270	276	PF00018	0.673
LIG_SH3_3	470	476	PF00018	0.620
LIG_SH3_3	579	585	PF00018	0.467
LIG_SH3_3	672	678	PF00018	0.500
LIG_SH3_3	803	809	PF00018	0.412
LIG_SH3_CIN85_PxpxPR_1	22	27	PF14604	0.694
LIG_SUMO_SIM_anti_2	370	376	PF11976	0.331
LIG_SUMO_SIM_anti_2	580	585	PF11976	0.576
LIG_SUMO_SIM_par_1	281	286	PF11976	0.364
LIG_SUMO_SIM_par_1	370	376	PF11976	0.294
LIG_SUMO_SIM_par_1	663	669	PF11976	0.696
LIG_SUMO_SIM_par_1	851	856	PF11976	0.368
MOD_CDK_SPxK_1	437	443	PF00069	0.408
MOD_CDK_SPxK_1	507	513	PF00069	0.522
MOD_CDK_SPxK_1	729	735	PF00069	0.499
MOD_CK1_1	179	185	PF00069	0.691
MOD_CK1_1	305	311	PF00069	0.378
MOD_CK1_1	628	634	PF00069	0.747
MOD_CK1_1	68	74	PF00069	0.767
MOD_CK1_1	753	759	PF00069	0.404
MOD_CK2_1	132	138	PF00069	0.541
MOD_CK2_1	198	204	PF00069	0.567
MOD_CK2_1	677	683	PF00069	0.533
MOD_CK2_1	698	704	PF00069	0.574
MOD_CK2_1	892	898	PF00069	0.424
MOD_GlcNHglycan	14	17	PF01048	0.730
MOD_GlcNHglycan	179	182	PF01048	0.673
MOD_GlcNHglycan	196	199	PF01048	0.758
MOD_GlcNHglycan	304	307	PF01048	0.371
MOD_GlcNHglycan	524	527	PF01048	0.528
MOD_GlcNHglycan	621	624	PF01048	0.763
MOD_GlcNHglycan	631	634	PF01048	0.730
MOD_GlcNHglycan	648	651	PF01048	0.726
MOD_GlcNHglycan	679	682	PF01048	0.565
MOD_GlcNHglycan	894	897	PF01048	0.404
MOD_GlcNHglycan	90	93	PF01048	0.608
MOD_GSK3_1	12	19	PF00069	0.710
MOD_GSK3_1	175	182	PF00069	0.732
MOD_GSK3_1	189	196	PF00069	0.660
MOD_GSK3_1	39	46	PF00069	0.747
MOD_GSK3_1	414	421	PF00069	0.382
MOD_GSK3_1	475	482	PF00069	0.542
MOD_GSK3_1	518	525	PF00069	0.542
MOD_GSK3_1	625	632	PF00069	0.727
MOD_GSK3_1	644	651	PF00069	0.515
MOD_GSK3_1	66	73	PF00069	0.699
MOD_GSK3_1	665	672	PF00069	0.705
MOD_GSK3_1	673	680	PF00069	0.600
MOD_GSK3_1	711	718	PF00069	0.645
MOD_GSK3_1	746	753	PF00069	0.462
MOD_GSK3_1	76	83	PF00069	0.451
MOD_GSK3_1	845	852	PF00069	0.396
MOD_N-GLC_1	152	157	PF02516	0.456
MOD_N-GLC_1	476	481	PF02516	0.536
MOD_N-GLC_1	746	751	PF02516	0.469
MOD_N-GLC_1	753	758	PF02516	0.419
MOD_NEK2_1	106	111	PF00069	0.464
MOD_NEK2_1	279	284	PF00069	0.558
MOD_NEK2_1	330	335	PF00069	0.324
MOD_NEK2_1	380	385	PF00069	0.320
MOD_NEK2_1	43	48	PF00069	0.733
MOD_NEK2_1	518	523	PF00069	0.474
MOD_NEK2_1	748	753	PF00069	0.451
MOD_NEK2_1	815	820	PF00069	0.600
MOD_NEK2_1	849	854	PF00069	0.380
MOD_NEK2_1	94	99	PF00069	0.549
MOD_NEK2_2	342	347	PF00069	0.354
MOD_NEK2_2	656	661	PF00069	0.691
MOD_NEK2_2	766	771	PF00069	0.514
MOD_NEK2_2	837	842	PF00069	0.373
MOD_PIKK_1	146	152	PF00454	0.602
MOD_PIKK_1	216	222	PF00454	0.599
MOD_PIKK_1	501	507	PF00454	0.554
MOD_PKA_1	673	679	PF00069	0.629
MOD_PKA_2	107	113	PF00069	0.481
MOD_PKA_2	176	182	PF00069	0.656
MOD_PKA_2	194	200	PF00069	0.729
MOD_PKA_2	43	49	PF00069	0.740
MOD_PKA_2	629	635	PF00069	0.768
MOD_PKA_2	673	679	PF00069	0.594
MOD_PKA_2	772	778	PF00069	0.649
MOD_PKA_2	892	898	PF00069	0.477
MOD_PKA_2	906	912	PF00069	0.525
MOD_PKA_2	919	925	PF00069	0.403
MOD_PKA_2	94	100	PF00069	0.551
MOD_PKB_1	671	679	PF00069	0.616
MOD_Plk_1	375	381	PF00069	0.335
MOD_Plk_1	418	424	PF00069	0.381
MOD_Plk_1	476	482	PF00069	0.488
MOD_Plk_1	766	772	PF00069	0.544
MOD_Plk_2-3	375	381	PF00069	0.335
MOD_Plk_4	308	314	PF00069	0.370
MOD_Plk_4	375	381	PF00069	0.324
MOD_Plk_4	766	772	PF00069	0.529
MOD_Plk_4	818	824	PF00069	0.479
MOD_Plk_4	849	855	PF00069	0.376
MOD_Plk_4	919	925	PF00069	0.407
MOD_Plk_4	94	100	PF00069	0.499
MOD_ProDKin_1	132	138	PF00069	0.541
MOD_ProDKin_1	269	275	PF00069	0.575
MOD_ProDKin_1	397	403	PF00069	0.373
MOD_ProDKin_1	437	443	PF00069	0.398
MOD_ProDKin_1	507	513	PF00069	0.475
MOD_ProDKin_1	57	63	PF00069	0.733
MOD_ProDKin_1	729	735	PF00069	0.481
MOD_ProDKin_1	74	80	PF00069	0.543
MOD_ProDKin_1	911	917	PF00069	0.620
MOD_SUMO_rev_2	490	499	PF00179	0.428
TRG_DiLeu_BaLyEn_6	543	548	PF01217	0.642
TRG_DiLeu_BaLyEn_6	796	801	PF01217	0.420
TRG_ENDOCYTIC_2	123	126	PF00928	0.578
TRG_ENDOCYTIC_2	258	261	PF00928	0.462
TRG_ENDOCYTIC_2	318	321	PF00928	0.479
TRG_ENDOCYTIC_2	425	428	PF00928	0.461
TRG_ENDOCYTIC_2	90	93	PF00928	0.582
TRG_ER_diArg_1	156	158	PF00400	0.665
TRG_ER_diArg_1	226	228	PF00400	0.566
TRG_ER_diArg_1	457	459	PF00400	0.488
TRG_ER_diArg_1	486	489	PF00400	0.457
TRG_ER_diArg_1	598	601	PF00400	0.416
TRG_ER_diArg_1	671	674	PF00400	0.700
TRG_ER_diArg_1	772	774	PF00400	0.488
TRG_ER_diArg_1	892	894	PF00400	0.572
TRG_NLS_MonoExtC_3	669	674	PF00514	0.740
TRG_Pf-PMV_PEXEL_1	799	804	PF00026	0.366
TRG_Pf-PMV_PEXEL_1	875	879	PF00026	0.466

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCJ6	Leptomonas seymouri	59%	84%
A0A1X0NSB2	Trypanosomatidae	51%	100%
A0A3Q8IGY7	Leishmania donovani	92%	100%
A0A422NKI2	Trypanosoma rangeli	50%	100%
A4HGV5	Leishmania braziliensis	79%	100%
A4I3Y5	Leishmania infantum	92%	100%
D0A956	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
E9B075	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q383A1	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	48%	100%
V5BL79	Trypanosoma cruzi	50%	100%