RF_PROK_I domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RF-1 domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4Q7J7_LEIMA

TriTrypDb:

LmjF.30.1060 , LMJLV39_300017000 , LMJSD75_300016600

Length:

166

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0000315	organellar large ribosomal subunit	5	2
GO:0005762	mitochondrial large ribosomal subunit	3	2
GO:0015934	large ribosomal subunit	4	2
GO:0032991	protein-containing complex	1	2
GO:0044391	ribosomal subunit	3	2
GO:0098798	mitochondrial protein-containing complex	2	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

Q4Q7J7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q7J7

Function

Biological processes
Term	Name	Level	Count
GO:0006415	translational termination	6	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0022411	cellular component disassembly	4	2
GO:0032984	protein-containing complex disassembly	5	2
GO:0043933	protein-containing complex organization	4	2
GO:0070126	mitochondrial translational termination	7	2
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	9
GO:0003747	translation release factor activity	5	9
GO:0003824	catalytic activity	1	2
GO:0004045	aminoacyl-tRNA hydrolase activity	5	2
GO:0005488	binding	1	9
GO:0008079	translation termination factor activity	4	9
GO:0008135	translation factor activity, RNA binding	3	9
GO:0016150	translation release factor activity, codon nonspecific	6	2
GO:0016787	hydrolase activity	2	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2
GO:0045182	translation regulator activity	1	9
GO:0052689	carboxylic ester hydrolase activity	4	2
GO:0090079	translation regulator activity, nucleic acid binding	2	9
GO:0097159	organic cyclic compound binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	113	115	PF00675	0.297
CLV_NRD_NRD_1	148	150	PF00675	0.488
CLV_NRD_NRD_1	80	82	PF00675	0.325
CLV_PCSK_KEX2_1	147	149	PF00082	0.481
CLV_PCSK_KEX2_1	80	82	PF00082	0.333
CLV_PCSK_SKI1_1	103	107	PF00082	0.325
DOC_ANK_TNKS_1	41	48	PF00023	0.458
DOC_USP7_MATH_1	8	12	PF00917	0.703
DOC_WW_Pin1_4	22	27	PF00397	0.424
LIG_14-3-3_CanoR_1	114	120	PF00244	0.482
LIG_14-3-3_CanoR_1	156	166	PF00244	0.490
LIG_BIR_II_1	1	5	PF00653	0.494
LIG_BRCT_BRCA1_1	67	71	PF00533	0.498
LIG_FHA_1	91	97	PF00498	0.483
LIG_LIR_Apic_2	124	128	PF02991	0.458
LIG_LIR_Nem_3	17	23	PF02991	0.489
LIG_MLH1_MIPbox_1	67	71	PF16413	0.483
LIG_NRBOX	115	121	PF00104	0.525
LIG_PCNA_PIPBox_1	64	73	PF02747	0.483
LIG_RPA_C_Fungi	76	88	PF08784	0.356
LIG_SH2_STAT3	21	24	PF00017	0.480
LIG_SH3_3	131	137	PF00018	0.382
LIG_SUMO_SIM_par_1	93	98	PF11976	0.332
MOD_CDK_SPK_2	22	27	PF00069	0.424
MOD_CK1_1	11	17	PF00069	0.557
MOD_CK1_1	160	166	PF00069	0.613
MOD_CK1_1	2	8	PF00069	0.611
MOD_CK2_1	11	17	PF00069	0.531
MOD_CK2_1	95	101	PF00069	0.332
MOD_GlcNHglycan	103	106	PF01048	0.332
MOD_GlcNHglycan	159	162	PF01048	0.579
MOD_GlcNHglycan	43	46	PF01048	0.349
MOD_GSK3_1	135	142	PF00069	0.543
MOD_GSK3_1	37	44	PF00069	0.324
MOD_GSK3_1	48	55	PF00069	0.312
MOD_GSK3_1	8	15	PF00069	0.539
MOD_GSK3_1	86	93	PF00069	0.332
MOD_N-GLC_1	51	56	PF02516	0.276
MOD_NEK2_1	157	162	PF00069	0.481
MOD_NEK2_1	41	46	PF00069	0.392
MOD_PKA_1	149	155	PF00069	0.546
MOD_PKA_2	135	141	PF00069	0.555
MOD_PKA_2	155	161	PF00069	0.459
MOD_PKA_2	41	47	PF00069	0.376
MOD_PKA_2	8	14	PF00069	0.546
MOD_PKB_1	147	155	PF00069	0.559
MOD_Plk_4	115	121	PF00069	0.321
MOD_Plk_4	135	141	PF00069	0.460
MOD_Plk_4	91	97	PF00069	0.391
MOD_ProDKin_1	22	28	PF00069	0.428
MOD_SUMO_rev_2	138	146	PF00179	0.503
TRG_DiLeu_BaEn_2	134	140	PF01217	0.582
TRG_ENDOCYTIC_2	20	23	PF00928	0.460
TRG_ER_diArg_1	146	149	PF00400	0.549
TRG_ER_diArg_1	79	81	PF00400	0.391
TRG_NLS_MonoExtN_4	147	153	PF00514	0.481

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3M0	Leptomonas seymouri	78%	97%
A0A0S4IXK6	Bodo saltans	49%	79%
A0A3S7X372	Leishmania donovani	96%	82%
A4HI61	Leishmania braziliensis	85%	82%
A4I5D7	Leishmania infantum	96%	82%
C9ZQR8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	73%
E9B0N5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	93%