tRNA pseudouridine synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridine synthase A-like protein, putative

Species:

Leishmania major

UniProt:

Q4Q7E9_LEIMA

TriTrypDb:

LmjF.30.1550 , LMJLV39_300022100 , LMJSD75_300021800

Length:

498

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q7E9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q7E9

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	2
GO:0016556	mRNA modification	6	2
GO:0031119	tRNA pseudouridine synthesis	7	2
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1990481	mRNA pseudouridine synthesis	7	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	7
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140101	catalytic activity, acting on a tRNA	4	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	1
GO:0016835	carbon-oxygen lyase activity	3	1
GO:0016836	hydro-lyase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	360	364	PF00656	0.468
CLV_NRD_NRD_1	284	286	PF00675	0.278
CLV_NRD_NRD_1	355	357	PF00675	0.663
CLV_NRD_NRD_1	375	377	PF00675	0.591
CLV_NRD_NRD_1	381	383	PF00675	0.569
CLV_NRD_NRD_1	404	406	PF00675	0.492
CLV_NRD_NRD_1	59	61	PF00675	0.372
CLV_NRD_NRD_1	95	97	PF00675	0.429
CLV_PCSK_KEX2_1	375	377	PF00082	0.591
CLV_PCSK_KEX2_1	381	383	PF00082	0.681
CLV_PCSK_KEX2_1	406	408	PF00082	0.513
CLV_PCSK_KEX2_1	473	475	PF00082	0.413
CLV_PCSK_KEX2_1	95	97	PF00082	0.397
CLV_PCSK_PC1ET2_1	406	408	PF00082	0.569
CLV_PCSK_PC1ET2_1	473	475	PF00082	0.419
CLV_PCSK_PC7_1	371	377	PF00082	0.486
CLV_PCSK_SKI1_1	224	228	PF00082	0.325
CLV_PCSK_SKI1_1	407	411	PF00082	0.585
CLV_PCSK_SKI1_1	50	54	PF00082	0.441
CLV_PCSK_SKI1_1	61	65	PF00082	0.271
DEG_APCC_DBOX_1	406	414	PF00400	0.523
DEG_SPOP_SBC_1	2	6	PF00917	0.599
DEG_SPOP_SBC_1	424	428	PF00917	0.615
DOC_CYCLIN_RxL_1	92	103	PF00134	0.373
DOC_MAPK_gen_1	60	66	PF00069	0.300
DOC_MAPK_MEF2A_6	257	264	PF00069	0.272
DOC_MAPK_RevD_3	270	286	PF00069	0.184
DOC_PP2B_LxvP_1	270	273	PF13499	0.359
DOC_USP7_MATH_1	129	133	PF00917	0.428
DOC_USP7_MATH_1	2	6	PF00917	0.599
DOC_USP7_MATH_1	339	343	PF00917	0.515
DOC_WW_Pin1_4	29	34	PF00397	0.704
DOC_WW_Pin1_4	292	297	PF00397	0.291
LIG_14-3-3_CanoR_1	110	118	PF00244	0.344
LIG_14-3-3_CanoR_1	173	181	PF00244	0.314
LIG_14-3-3_CanoR_1	224	230	PF00244	0.365
LIG_14-3-3_CanoR_1	247	252	PF00244	0.322
LIG_14-3-3_CanoR_1	333	340	PF00244	0.434
LIG_Actin_WH2_2	82	100	PF00022	0.413
LIG_BIR_II_1	1	5	PF00653	0.762
LIG_BIR_III_2	136	140	PF00653	0.570
LIG_BIR_III_4	363	367	PF00653	0.510
LIG_BRCT_BRCA1_1	341	345	PF00533	0.437
LIG_deltaCOP1_diTrp_1	384	393	PF00928	0.471
LIG_EH1_1	253	261	PF00400	0.271
LIG_FHA_1	155	161	PF00498	0.308
LIG_FHA_1	286	292	PF00498	0.316
LIG_FHA_1	293	299	PF00498	0.316
LIG_FHA_1	326	332	PF00498	0.409
LIG_FHA_1	349	355	PF00498	0.441
LIG_FHA_2	276	282	PF00498	0.394
LIG_FHA_2	387	393	PF00498	0.522
LIG_FHA_2	4	10	PF00498	0.563
LIG_FHA_2	425	431	PF00498	0.738
LIG_FHA_2	459	465	PF00498	0.424
LIG_LIR_Apic_2	392	396	PF02991	0.548
LIG_LIR_Gen_1	145	154	PF02991	0.342
LIG_LIR_Gen_1	171	181	PF02991	0.340
LIG_LIR_Gen_1	242	251	PF02991	0.284
LIG_LIR_Gen_1	301	311	PF02991	0.459
LIG_LIR_Nem_3	171	177	PF02991	0.336
LIG_LIR_Nem_3	180	186	PF02991	0.293
LIG_LIR_Nem_3	205	211	PF02991	0.349
LIG_LIR_Nem_3	242	246	PF02991	0.263
LIG_LIR_Nem_3	250	256	PF02991	0.294
LIG_LIR_Nem_3	301	307	PF02991	0.273
LIG_LIR_Nem_3	342	348	PF02991	0.454
LIG_MYND_1	139	143	PF01753	0.502
LIG_Pex14_1	300	304	PF04695	0.256
LIG_Pex14_2	170	174	PF04695	0.312
LIG_REV1ctd_RIR_1	183	192	PF16727	0.368
LIG_SH2_CRK	253	257	PF00017	0.256
LIG_SH2_PTP2	450	453	PF00017	0.434
LIG_SH2_SRC	243	246	PF00017	0.357
LIG_SH2_STAP1	462	466	PF00017	0.521
LIG_SH2_STAT5	146	149	PF00017	0.305
LIG_SH2_STAT5	184	187	PF00017	0.307
LIG_SH2_STAT5	243	246	PF00017	0.292
LIG_SH2_STAT5	348	351	PF00017	0.493
LIG_SH2_STAT5	412	415	PF00017	0.630
LIG_SH2_STAT5	450	453	PF00017	0.374
LIG_SH3_3	136	142	PF00018	0.500
LIG_SH3_3	284	290	PF00018	0.256
LIG_SH3_5	142	146	PF00018	0.369
LIG_SUMO_SIM_par_1	278	284	PF11976	0.390
LIG_SUMO_SIM_par_1	295	301	PF11976	0.208
LIG_TRAF2_1	194	197	PF00917	0.441
LIG_TRAF2_1	428	431	PF00917	0.631
LIG_TRAF2_1	461	464	PF00917	0.490
LIG_TYR_ITIM	241	246	PF00017	0.272
LIG_TYR_ITIM	448	453	PF00017	0.425
LIG_UBA3_1	279	286	PF00899	0.271
LIG_UBA3_1	409	418	PF00899	0.479
MOD_CK1_1	19	25	PF00069	0.686
MOD_CK1_1	3	9	PF00069	0.706
MOD_CK1_1	32	38	PF00069	0.549
MOD_CK2_1	239	245	PF00069	0.261
MOD_CK2_1	275	281	PF00069	0.394
MOD_CK2_1	424	430	PF00069	0.687
MOD_CK2_1	458	464	PF00069	0.395
MOD_Cter_Amidation	470	473	PF01082	0.546
MOD_GlcNHglycan	118	121	PF01048	0.336
MOD_GlcNHglycan	19	22	PF01048	0.579
MOD_GlcNHglycan	335	338	PF01048	0.496
MOD_GlcNHglycan	363	367	PF01048	0.698
MOD_GlcNHglycan	41	44	PF01048	0.648
MOD_GlcNHglycan	441	444	PF01048	0.622
MOD_GlcNHglycan	468	472	PF01048	0.481
MOD_GlcNHglycan	8	13	PF01048	0.612
MOD_GSK3_1	125	132	PF00069	0.324
MOD_GSK3_1	19	26	PF00069	0.689
MOD_GSK3_1	271	278	PF00069	0.394
MOD_GSK3_1	335	342	PF00069	0.480
MOD_GSK3_1	391	398	PF00069	0.564
MOD_N-GLC_1	217	222	PF02516	0.305
MOD_N-GLC_2	210	212	PF02516	0.256
MOD_NEK2_1	1	6	PF00069	0.741
MOD_NEK2_1	125	130	PF00069	0.355
MOD_NEK2_1	202	207	PF00069	0.349
MOD_NEK2_1	222	227	PF00069	0.140
MOD_PIKK_1	339	345	PF00454	0.413
MOD_PIKK_1	431	437	PF00454	0.764
MOD_PKA_1	285	291	PF00069	0.271
MOD_PKA_1	356	362	PF00069	0.558
MOD_PKA_2	129	135	PF00069	0.350
MOD_PKA_2	172	178	PF00069	0.306
MOD_PKA_2	332	338	PF00069	0.414
MOD_PKA_2	455	461	PF00069	0.393
MOD_Plk_1	458	464	PF00069	0.407
MOD_Plk_1	8	14	PF00069	0.560
MOD_Plk_1	82	88	PF00069	0.466
MOD_Plk_2-3	239	245	PF00069	0.256
MOD_Plk_4	145	151	PF00069	0.409
MOD_Plk_4	247	253	PF00069	0.346
MOD_Plk_4	271	277	PF00069	0.355
MOD_ProDKin_1	29	35	PF00069	0.705
MOD_ProDKin_1	292	298	PF00069	0.291
MOD_SUMO_rev_2	281	288	PF00179	0.261
MOD_SUMO_rev_2	428	434	PF00179	0.663
MOD_SUMO_rev_2	478	485	PF00179	0.451
TRG_DiLeu_BaEn_1	261	266	PF01217	0.294
TRG_DiLeu_BaEn_4	464	470	PF01217	0.411
TRG_DiLeu_BaLyEn_6	293	298	PF01217	0.353
TRG_DiLeu_BaLyEn_6	449	454	PF01217	0.537
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.335
TRG_ENDOCYTIC_2	146	149	PF00928	0.320
TRG_ENDOCYTIC_2	183	186	PF00928	0.435
TRG_ENDOCYTIC_2	243	246	PF00928	0.263
TRG_ENDOCYTIC_2	253	256	PF00928	0.243
TRG_ENDOCYTIC_2	450	453	PF00928	0.374
TRG_ER_diArg_1	374	376	PF00400	0.599
TRG_ER_diArg_1	380	382	PF00400	0.571
TRG_ER_diArg_1	474	477	PF00400	0.402
TRG_ER_diArg_1	94	96	PF00400	0.350
TRG_NLS_MonoExtC_3	471	476	PF00514	0.410
TRG_NLS_MonoExtN_4	472	477	PF00514	0.408
TRG_Pf-PMV_PEXEL_1	257	261	PF00026	0.348
TRG_Pf-PMV_PEXEL_1	98	102	PF00026	0.382

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA61	Leptomonas seymouri	70%	97%
A0A0S4JB03	Bodo saltans	50%	100%
A0A1X0P1U9	Trypanosomatidae	58%	100%
A0A3S7X3E8	Leishmania donovani	95%	100%
A0A422P3K1	Trypanosoma rangeli	59%	100%
A4HIA0	Leishmania braziliensis	83%	100%
A4I5I4	Leishmania infantum	94%	100%
C9ZQW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9B0S9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q09524	Caenorhabditis elegans	34%	100%
V5DQN1	Trypanosoma cruzi	59%	94%