Putative splicing factor 3a

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

splicing factor 3a, putative

Species:

Leishmania major

UniProt:

Q4Q7B2_LEIMA

TriTrypDb:

LmjF.30.1830 , LMJLV39_300024600 , LMJSD75_300024400

Length:

622

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	11
GO:1990904	ribonucleoprotein complex	2	11

Expansion

Sequence features

Q4Q7B2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q7B2

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	11
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	11
GO:0000398	mRNA splicing, via spliceosome	8	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006397	mRNA processing	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0008380	RNA splicing	7	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0016071	mRNA metabolic process	6	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	10
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	10
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	432	436	PF00656	0.493
CLV_C14_Caspase3-7	594	598	PF00656	0.420
CLV_C14_Caspase3-7	600	604	PF00656	0.302
CLV_C14_Caspase3-7	79	83	PF00656	0.403
CLV_NRD_NRD_1	210	212	PF00675	0.342
CLV_NRD_NRD_1	235	237	PF00675	0.537
CLV_NRD_NRD_1	440	442	PF00675	0.588
CLV_NRD_NRD_1	471	473	PF00675	0.686
CLV_NRD_NRD_1	554	556	PF00675	0.302
CLV_NRD_NRD_1	588	590	PF00675	0.302
CLV_PCSK_KEX2_1	235	237	PF00082	0.482
CLV_PCSK_KEX2_1	471	473	PF00082	0.680
CLV_PCSK_KEX2_1	554	556	PF00082	0.309
CLV_PCSK_KEX2_1	590	592	PF00082	0.306
CLV_PCSK_KEX2_1	620	622	PF00082	0.574
CLV_PCSK_PC1ET2_1	590	592	PF00082	0.306
CLV_PCSK_PC7_1	616	622	PF00082	0.440
CLV_PCSK_SKI1_1	39	43	PF00082	0.463
CLV_PCSK_SKI1_1	461	465	PF00082	0.630
DEG_COP1_1	113	120	PF00400	0.496
DEG_Nend_UBRbox_3	1	3	PF02207	0.635
DEG_SPOP_SBC_1	306	310	PF00917	0.738
DEG_SPOP_SBC_1	369	373	PF00917	0.608
DOC_CYCLIN_RxL_1	36	46	PF00134	0.464
DOC_CYCLIN_yClb1_LxF_4	187	192	PF00134	0.518
DOC_CYCLIN_yCln2_LP_2	218	224	PF00134	0.400
DOC_MAPK_DCC_7	341	350	PF00069	0.504
DOC_MAPK_MEF2A_6	197	204	PF00069	0.486
DOC_PP1_RVXF_1	187	193	PF00149	0.517
DOC_PP1_RVXF_1	210	217	PF00149	0.474
DOC_PP2B_LxvP_1	115	118	PF13499	0.518
DOC_PP2B_LxvP_1	330	333	PF13499	0.408
DOC_USP7_MATH_1	278	282	PF00917	0.704
DOC_USP7_MATH_1	305	309	PF00917	0.817
DOC_USP7_MATH_1	368	372	PF00917	0.693
DOC_WW_Pin1_4	116	121	PF00397	0.522
DOC_WW_Pin1_4	172	177	PF00397	0.588
DOC_WW_Pin1_4	77	82	PF00397	0.609
LIG_14-3-3_CanoR_1	145	151	PF00244	0.486
LIG_14-3-3_CanoR_1	441	448	PF00244	0.475
LIG_14-3-3_CanoR_1	54	61	PF00244	0.496
LIG_Actin_WH2_2	445	463	PF00022	0.566
LIG_APCC_ABBA_1	61	66	PF00400	0.426
LIG_BIR_III_4	603	607	PF00653	0.321
LIG_CtBP_PxDLS_1	333	337	PF00389	0.488
LIG_FHA_1	1	7	PF00498	0.573
LIG_FHA_1	151	157	PF00498	0.523
LIG_FHA_1	194	200	PF00498	0.472
LIG_FHA_1	370	376	PF00498	0.481
LIG_FHA_1	407	413	PF00498	0.344
LIG_FHA_2	126	132	PF00498	0.438
LIG_FHA_2	153	159	PF00498	0.514
LIG_FHA_2	280	286	PF00498	0.651
LIG_FHA_2	400	406	PF00498	0.430
LIG_FHA_2	416	422	PF00498	0.327
LIG_FHA_2	580	586	PF00498	0.388
LIG_FHA_2	594	600	PF00498	0.420
LIG_FHA_2	608	614	PF00498	0.420
LIG_KLC1_Yacidic_2	62	66	PF13176	0.426
LIG_LIR_Apic_2	344	349	PF02991	0.480
LIG_LIR_Apic_2	532	537	PF02991	0.279
LIG_LIR_Gen_1	100	109	PF02991	0.558
LIG_LIR_Gen_1	257	268	PF02991	0.396
LIG_LIR_Gen_1	398	405	PF02991	0.389
LIG_LIR_Nem_3	100	104	PF02991	0.445
LIG_LIR_Nem_3	140	146	PF02991	0.359
LIG_LIR_Nem_3	191	195	PF02991	0.390
LIG_LIR_Nem_3	231	237	PF02991	0.455
LIG_LIR_Nem_3	257	263	PF02991	0.383
LIG_LIR_Nem_3	398	404	PF02991	0.386
LIG_LIR_Nem_3	564	568	PF02991	0.321
LIG_LIR_Nem_3	613	617	PF02991	0.420
LIG_NRP_CendR_1	620	622	PF00754	0.651
LIG_Pex14_2	540	544	PF04695	0.302
LIG_RPA_C_Fungi	206	218	PF08784	0.442
LIG_SH2_CRK	234	238	PF00017	0.372
LIG_SH2_CRK	534	538	PF00017	0.427
LIG_SH2_SRC	229	232	PF00017	0.443
LIG_SH2_STAP1	146	150	PF00017	0.348
LIG_SH2_STAP1	415	419	PF00017	0.457
LIG_SH2_STAT5	229	232	PF00017	0.334
LIG_SH2_STAT5	401	404	PF00017	0.505
LIG_SH2_STAT5	614	617	PF00017	0.483
LIG_SH2_STAT5	64	67	PF00017	0.421
LIG_SH3_1	236	242	PF00018	0.491
LIG_SH3_3	114	120	PF00018	0.579
LIG_SH3_3	170	176	PF00018	0.614
LIG_SH3_3	236	242	PF00018	0.491
LIG_SH3_3	485	491	PF00018	0.760
LIG_TRAF2_1	418	421	PF00917	0.479
LIG_TRAF2_1	429	432	PF00917	0.445
LIG_TRAF2_1	436	439	PF00917	0.425
LIG_TRAF2_1	494	497	PF00917	0.651
LIG_TRFH_1	343	347	PF08558	0.354
LIG_WRC_WIRS_1	192	197	PF05994	0.441
LIG_WRC_WIRS_1	396	401	PF05994	0.311
LIG_WRPW_2	219	222	PF00400	0.398
MOD_CDK_SPK_2	116	121	PF00069	0.460
MOD_CK1_1	175	181	PF00069	0.556
MOD_CK1_1	362	368	PF00069	0.704
MOD_CK1_1	444	450	PF00069	0.514
MOD_CK1_1	467	473	PF00069	0.683
MOD_CK2_1	125	131	PF00069	0.388
MOD_CK2_1	152	158	PF00069	0.501
MOD_CK2_1	251	257	PF00069	0.420
MOD_CK2_1	259	265	PF00069	0.440
MOD_CK2_1	279	285	PF00069	0.437
MOD_CK2_1	399	405	PF00069	0.430
MOD_CK2_1	415	421	PF00069	0.333
MOD_CK2_1	433	439	PF00069	0.424
MOD_CK2_1	52	58	PF00069	0.480
MOD_CK2_1	579	585	PF00069	0.467
MOD_CK2_1	593	599	PF00069	0.420
MOD_CK2_1	607	613	PF00069	0.420
MOD_GlcNHglycan	146	149	PF01048	0.482
MOD_GlcNHglycan	161	164	PF01048	0.637
MOD_GlcNHglycan	361	364	PF01048	0.662
MOD_GlcNHglycan	443	446	PF01048	0.491
MOD_GlcNHglycan	478	481	PF01048	0.743
MOD_GlcNHglycan	585	588	PF01048	0.367
MOD_GSK3_1	269	276	PF00069	0.486
MOD_GSK3_1	307	314	PF00069	0.733
MOD_GSK3_1	370	377	PF00069	0.566
MOD_GSK3_1	395	402	PF00069	0.461
MOD_GSK3_1	403	410	PF00069	0.404
MOD_GSK3_1	444	451	PF00069	0.447
MOD_GSK3_1	579	586	PF00069	0.346
MOD_LATS_1	339	345	PF00433	0.345
MOD_N-GLC_1	407	412	PF02516	0.515
MOD_NEK2_1	150	155	PF00069	0.406
MOD_NEK2_1	180	185	PF00069	0.584
MOD_NEK2_1	193	198	PF00069	0.437
MOD_NEK2_1	395	400	PF00069	0.382
MOD_NEK2_1	404	409	PF00069	0.391
MOD_NEK2_1	448	453	PF00069	0.555
MOD_NEK2_1	464	469	PF00069	0.617
MOD_NEK2_2	529	534	PF00069	0.294
MOD_NEK2_2	567	572	PF00069	0.302
MOD_PKA_1	341	347	PF00069	0.440
MOD_PKA_1	441	447	PF00069	0.512
MOD_PKA_2	144	150	PF00069	0.377
MOD_PKA_2	53	59	PF00069	0.517
MOD_PKB_1	591	599	PF00069	0.420
MOD_Plk_1	268	274	PF00069	0.563
MOD_Plk_1	407	413	PF00069	0.528
MOD_Plk_2-3	125	131	PF00069	0.507
MOD_Plk_2-3	251	257	PF00069	0.465
MOD_Plk_4	175	181	PF00069	0.568
MOD_Plk_4	19	25	PF00069	0.439
MOD_Plk_4	259	265	PF00069	0.533
MOD_Plk_4	395	401	PF00069	0.403
MOD_Plk_4	407	413	PF00069	0.381
MOD_Plk_4	529	535	PF00069	0.279
MOD_ProDKin_1	116	122	PF00069	0.517
MOD_ProDKin_1	172	178	PF00069	0.588
MOD_ProDKin_1	77	83	PF00069	0.604
MOD_SUMO_for_1	111	114	PF00179	0.531
MOD_SUMO_for_1	167	170	PF00179	0.557
MOD_SUMO_for_1	266	269	PF00179	0.514
MOD_SUMO_rev_2	262	268	PF00179	0.451
MOD_SUMO_rev_2	378	385	PF00179	0.419
MOD_SUMO_rev_2	465	470	PF00179	0.572
MOD_SUMO_rev_2	90	96	PF00179	0.533
TRG_DiLeu_BaEn_1	498	503	PF01217	0.678
TRG_DiLeu_BaLyEn_6	118	123	PF01217	0.504
TRG_DiLeu_BaLyEn_6	346	351	PF01217	0.487
TRG_ENDOCYTIC_2	101	104	PF00928	0.478
TRG_ENDOCYTIC_2	105	108	PF00928	0.569
TRG_ENDOCYTIC_2	146	149	PF00928	0.349
TRG_ENDOCYTIC_2	219	222	PF00928	0.357
TRG_ENDOCYTIC_2	234	237	PF00928	0.368
TRG_ENDOCYTIC_2	401	404	PF00928	0.505
TRG_ENDOCYTIC_2	565	568	PF00928	0.321
TRG_ENDOCYTIC_2	614	617	PF00928	0.321
TRG_ER_diArg_1	234	236	PF00400	0.473
TRG_ER_diArg_1	37	40	PF00400	0.470
TRG_ER_diArg_1	471	474	PF00400	0.693
TRG_ER_diArg_1	553	555	PF00400	0.302
TRG_ER_diArg_1	588	591	PF00400	0.304
TRG_ER_diArg_1	620	622	PF00400	0.671
TRG_NES_CRM1_1	566	579	PF08389	0.390
TRG_NLS_MonoExtC_3	588	593	PF00514	0.321
TRG_Pf-PMV_PEXEL_1	10	15	PF00026	0.515
TRG_Pf-PMV_PEXEL_1	121	125	PF00026	0.515
TRG_Pf-PMV_PEXEL_1	154	158	PF00026	0.528
TRG_Pf-PMV_PEXEL_1	39	43	PF00026	0.457
TRG_Pf-PMV_PEXEL_1	417	421	PF00026	0.564
TRG_Pf-PMV_PEXEL_1	512	516	PF00026	0.380
TRG_PTS2	1	45	PF00400	0.407

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5M4	Leptomonas seymouri	62%	97%
A0A1X0P1R3	Trypanosomatidae	41%	100%
A0A3S7X3F0	Leishmania donovani	95%	100%
A0A422P3I2	Trypanosoma rangeli	44%	100%
A4H930	Leishmania braziliensis	84%	100%
A4I5K9	Leishmania infantum	95%	100%
C9ZQY7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9B0V4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5DQL3	Trypanosoma cruzi	42%	100%