GRAM domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4Q722_LEIMA

TriTrypDb:

LmjF.30.2720 , LMJLV39_300034800 , LMJSD75_300034200

Length:

731

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	2
GO:0031090	organelle membrane	3	2
GO:0044232	organelle membrane contact site	2	2
GO:0110165	cellular anatomical entity	1	2
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	2

Expansion

Sequence features

Q4Q722

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q722

PDB structure(s): 4tyz_A , 4tyz_B

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006869	lipid transport	5	2
GO:0009987	cellular process	1	2
GO:0015850	organic hydroxy compound transport	5	2
GO:0015918	sterol transport	6	2
GO:0032365	intracellular lipid transport	4	2
GO:0032366	intracellular sterol transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0071702	organic substance transport	4	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	2
GO:0005319	lipid transporter activity	2	2
GO:0005488	binding	1	2
GO:0005496	steroid binding	3	2
GO:0008289	lipid binding	2	2
GO:0015248	sterol transporter activity	3	2
GO:0032934	sterol binding	4	2
GO:0097159	organic cyclic compound binding	2	2
GO:0120013	lipid transfer activity	3	2
GO:0120015	sterol transfer activity	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	474	478	PF00656	0.420
CLV_C14_Caspase3-7	521	525	PF00656	0.497
CLV_C14_Caspase3-7	638	642	PF00656	0.357
CLV_NRD_NRD_1	13	15	PF00675	0.382
CLV_NRD_NRD_1	185	187	PF00675	0.692
CLV_NRD_NRD_1	222	224	PF00675	0.745
CLV_NRD_NRD_1	435	437	PF00675	0.440
CLV_NRD_NRD_1	535	537	PF00675	0.503
CLV_NRD_NRD_1	627	629	PF00675	0.297
CLV_PCSK_FUR_1	533	537	PF00082	0.501
CLV_PCSK_KEX2_1	13	15	PF00082	0.382
CLV_PCSK_KEX2_1	185	187	PF00082	0.698
CLV_PCSK_KEX2_1	221	223	PF00082	0.674
CLV_PCSK_KEX2_1	435	437	PF00082	0.440
CLV_PCSK_KEX2_1	535	537	PF00082	0.503
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.570
CLV_PCSK_SKI1_1	134	138	PF00082	0.522
CLV_PCSK_SKI1_1	225	229	PF00082	0.635
CLV_PCSK_SKI1_1	293	297	PF00082	0.651
CLV_PCSK_SKI1_1	545	549	PF00082	0.777
CLV_PCSK_SKI1_1	650	654	PF00082	0.258
CLV_PCSK_SKI1_1	690	694	PF00082	0.312
DEG_COP1_1	156	166	PF00400	0.554
DEG_SPOP_SBC_1	143	147	PF00917	0.541
DEG_SPOP_SBC_1	239	243	PF00917	0.588
DOC_CKS1_1	659	664	PF01111	0.297
DOC_CYCLIN_yCln2_LP_2	595	601	PF00134	0.582
DOC_MAPK_DCC_7	315	323	PF00069	0.380
DOC_MAPK_gen_1	4	11	PF00069	0.465
DOC_MAPK_MEF2A_6	315	323	PF00069	0.369
DOC_PP1_RVXF_1	543	550	PF00149	0.615
DOC_PP2B_LxvP_1	595	598	PF13499	0.571
DOC_PP4_FxxP_1	402	405	PF00568	0.403
DOC_PP4_FxxP_1	560	563	PF00568	0.632
DOC_PP4_FxxP_1	659	662	PF00568	0.339
DOC_USP7_MATH_1	153	157	PF00917	0.642
DOC_USP7_MATH_1	176	180	PF00917	0.834
DOC_USP7_MATH_1	233	237	PF00917	0.719
DOC_USP7_MATH_1	240	244	PF00917	0.748
DOC_USP7_MATH_1	274	278	PF00917	0.614
DOC_USP7_MATH_1	34	38	PF00917	0.553
DOC_USP7_MATH_1	529	533	PF00917	0.518
DOC_USP7_MATH_1	56	60	PF00917	0.523
DOC_USP7_MATH_1	723	727	PF00917	0.371
DOC_USP7_UBL2_3	220	224	PF12436	0.635
DOC_USP7_UBL2_3	684	688	PF12436	0.357
DOC_WW_Pin1_4	155	160	PF00397	0.644
DOC_WW_Pin1_4	303	308	PF00397	0.600
DOC_WW_Pin1_4	4	9	PF00397	0.546
DOC_WW_Pin1_4	401	406	PF00397	0.420
DOC_WW_Pin1_4	448	453	PF00397	0.382
DOC_WW_Pin1_4	658	663	PF00397	0.297
LIG_14-3-3_CanoR_1	27	36	PF00244	0.527
LIG_14-3-3_CanoR_1	435	441	PF00244	0.393
LIG_14-3-3_CanoR_1	628	632	PF00244	0.299
LIG_14-3-3_CanoR_1	63	67	PF00244	0.421
LIG_Actin_WH2_2	26	43	PF00022	0.502
LIG_AP2alpha_2	400	402	PF02296	0.477
LIG_BIR_II_1	1	5	PF00653	0.628
LIG_BIR_III_4	329	333	PF00653	0.446
LIG_BIR_III_4	611	615	PF00653	0.508
LIG_eIF4E_1	438	444	PF01652	0.360
LIG_FHA_1	158	164	PF00498	0.557
LIG_FHA_1	24	30	PF00498	0.462
LIG_FHA_1	322	328	PF00498	0.514
LIG_FHA_1	355	361	PF00498	0.404
LIG_FHA_1	451	457	PF00498	0.407
LIG_FHA_1	484	490	PF00498	0.430
LIG_FHA_1	493	499	PF00498	0.555
LIG_FHA_1	50	56	PF00498	0.675
LIG_FHA_1	515	521	PF00498	0.450
LIG_FHA_1	549	555	PF00498	0.597
LIG_FHA_1	614	620	PF00498	0.550
LIG_FHA_1	706	712	PF00498	0.362
LIG_FHA_2	229	235	PF00498	0.655
LIG_FHA_2	265	271	PF00498	0.546
LIG_FHA_2	304	310	PF00498	0.536
LIG_FHA_2	324	330	PF00498	0.512
LIG_FHA_2	348	354	PF00498	0.454
LIG_FHA_2	381	387	PF00498	0.467
LIG_FHA_2	472	478	PF00498	0.416
LIG_FHA_2	5	11	PF00498	0.516
LIG_FHA_2	539	545	PF00498	0.486
LIG_FHA_2	636	642	PF00498	0.357
LIG_LIR_Apic_2	348	354	PF02991	0.536
LIG_LIR_Apic_2	400	405	PF02991	0.413
LIG_LIR_Apic_2	557	563	PF02991	0.624
LIG_LIR_Gen_1	317	327	PF02991	0.409
LIG_LIR_Gen_1	356	365	PF02991	0.361
LIG_LIR_Gen_1	439	448	PF02991	0.399
LIG_LIR_Gen_1	481	489	PF02991	0.433
LIG_LIR_Gen_1	517	527	PF02991	0.444
LIG_LIR_Gen_1	661	672	PF02991	0.314
LIG_LIR_Gen_1	677	687	PF02991	0.227
LIG_LIR_Gen_1	718	729	PF02991	0.298
LIG_LIR_Nem_3	248	254	PF02991	0.644
LIG_LIR_Nem_3	317	323	PF02991	0.400
LIG_LIR_Nem_3	356	362	PF02991	0.360
LIG_LIR_Nem_3	367	373	PF02991	0.322
LIG_LIR_Nem_3	439	444	PF02991	0.359
LIG_LIR_Nem_3	481	485	PF02991	0.430
LIG_LIR_Nem_3	517	522	PF02991	0.436
LIG_LIR_Nem_3	630	634	PF02991	0.282
LIG_LIR_Nem_3	661	667	PF02991	0.314
LIG_LIR_Nem_3	677	683	PF02991	0.298
LIG_LIR_Nem_3	718	724	PF02991	0.357
LIG_PCNA_yPIPBox_3	352	364	PF02747	0.452
LIG_Pex14_2	104	108	PF04695	0.484
LIG_Pex14_2	627	631	PF04695	0.298
LIG_Pex14_2	643	647	PF04695	0.298
LIG_Pex14_2	710	714	PF04695	0.298
LIG_REV1ctd_RIR_1	135	143	PF16727	0.553
LIG_SH2_CRK	721	725	PF00017	0.346
LIG_SH2_NCK_1	116	120	PF00017	0.458
LIG_SH2_NCK_1	441	445	PF00017	0.412
LIG_SH2_PTP2	359	362	PF00017	0.348
LIG_SH2_SRC	680	683	PF00017	0.357
LIG_SH2_STAP1	209	213	PF00017	0.645
LIG_SH2_STAP1	438	442	PF00017	0.363
LIG_SH2_STAP1	721	725	PF00017	0.287
LIG_SH2_STAT5	320	323	PF00017	0.398
LIG_SH2_STAT5	359	362	PF00017	0.348
LIG_SH2_STAT5	370	373	PF00017	0.330
LIG_SH2_STAT5	376	379	PF00017	0.383
LIG_SH2_STAT5	438	441	PF00017	0.365
LIG_SH2_STAT5	657	660	PF00017	0.357
LIG_SH3_2	284	289	PF14604	0.699
LIG_SH3_3	199	205	PF00018	0.601
LIG_SH3_3	281	287	PF00018	0.714
LIG_SH3_3	544	550	PF00018	0.544
LIG_SUMO_SIM_anti_2	30	37	PF11976	0.516
LIG_SUMO_SIM_par_1	471	477	PF11976	0.423
LIG_TRAF2_1	550	553	PF00917	0.600
LIG_TRAF2_2	202	207	PF00917	0.553
LIG_TRAF2_2	550	555	PF00917	0.500
LIG_TYR_ITIM	678	683	PF00017	0.371
LIG_TYR_ITSM	437	444	PF00017	0.419
LIG_WRC_WIRS_1	105	110	PF05994	0.495
LIG_WRC_WIRS_1	506	511	PF05994	0.447
LIG_WRC_WIRS_1	640	645	PF05994	0.357
LIG_WW_3	446	450	PF00397	0.527
MOD_CK1_1	142	148	PF00069	0.631
MOD_CK1_1	2	8	PF00069	0.535
MOD_CK1_1	23	29	PF00069	0.458
MOD_CK1_1	257	263	PF00069	0.709
MOD_CK1_1	291	297	PF00069	0.695
MOD_CK1_1	344	350	PF00069	0.452
MOD_CK1_1	380	386	PF00069	0.561
MOD_CK1_1	59	65	PF00069	0.494
MOD_CK2_1	228	234	PF00069	0.681
MOD_CK2_1	322	328	PF00069	0.508
MOD_CK2_1	347	353	PF00069	0.459
MOD_CK2_1	4	10	PF00069	0.531
MOD_CK2_1	520	526	PF00069	0.472
MOD_CK2_1	71	77	PF00069	0.534
MOD_Cter_Amidation	183	186	PF01082	0.675
MOD_GlcNHglycan	1	4	PF01048	0.596
MOD_GlcNHglycan	211	214	PF01048	0.842
MOD_GlcNHglycan	234	238	PF01048	0.662
MOD_GlcNHglycan	262	265	PF01048	0.644
MOD_GlcNHglycan	276	279	PF01048	0.628
MOD_GlcNHglycan	379	382	PF01048	0.472
MOD_GlcNHglycan	511	514	PF01048	0.567
MOD_GlcNHglycan	649	653	PF01048	0.333
MOD_GlcNHglycan	721	724	PF01048	0.325
MOD_GSK3_1	103	110	PF00069	0.478
MOD_GSK3_1	139	146	PF00069	0.575
MOD_GSK3_1	147	154	PF00069	0.581
MOD_GSK3_1	172	179	PF00069	0.685
MOD_GSK3_1	23	30	PF00069	0.430
MOD_GSK3_1	235	242	PF00069	0.739
MOD_GSK3_1	256	263	PF00069	0.709
MOD_GSK3_1	274	281	PF00069	0.617
MOD_GSK3_1	341	348	PF00069	0.431
MOD_GSK3_1	45	52	PF00069	0.642
MOD_GSK3_1	488	495	PF00069	0.435
MOD_GSK3_1	505	512	PF00069	0.440
MOD_GSK3_1	55	62	PF00069	0.482
MOD_GSK3_1	635	642	PF00069	0.357
MOD_GSK3_1	67	74	PF00069	0.534
MOD_GSK3_1	705	712	PF00069	0.298
MOD_GSK3_1	719	726	PF00069	0.298
MOD_LATS_1	25	31	PF00433	0.505
MOD_N-GLC_1	20	25	PF02516	0.455
MOD_N-GLC_1	289	294	PF02516	0.644
MOD_N-GLC_1	344	349	PF02516	0.390
MOD_N-GLC_1	70	75	PF02516	0.416
MOD_N-GLC_1	705	710	PF02516	0.357
MOD_NEK2_1	104	109	PF00069	0.488
MOD_NEK2_1	141	146	PF00069	0.591
MOD_NEK2_1	321	326	PF00069	0.461
MOD_NEK2_1	507	512	PF00069	0.452
MOD_NEK2_1	514	519	PF00069	0.462
MOD_NEK2_1	554	559	PF00069	0.566
MOD_NEK2_1	610	615	PF00069	0.522
MOD_NEK2_1	627	632	PF00069	0.298
MOD_NEK2_1	639	644	PF00069	0.304
MOD_NEK2_1	667	672	PF00069	0.400
MOD_NEK2_1	705	710	PF00069	0.298
MOD_OFUCOSY	69	75	PF10250	0.483
MOD_PIKK_1	107	113	PF00454	0.479
MOD_PIKK_1	245	251	PF00454	0.677
MOD_PIKK_1	254	260	PF00454	0.545
MOD_PIKK_1	34	40	PF00454	0.559
MOD_PIKK_1	380	386	PF00454	0.477
MOD_PIKK_1	497	503	PF00454	0.465
MOD_PIKK_1	548	554	PF00454	0.494
MOD_PIKK_1	723	729	PF00454	0.371
MOD_PK_1	139	145	PF00069	0.543
MOD_PK_1	688	694	PF00069	0.371
MOD_PKA_1	225	231	PF00069	0.795
MOD_PKA_2	483	489	PF00069	0.555
MOD_PKA_2	62	68	PF00069	0.412
MOD_PKA_2	627	633	PF00069	0.299
MOD_Plk_1	139	145	PF00069	0.543
MOD_Plk_1	264	270	PF00069	0.615
MOD_Plk_1	344	350	PF00069	0.395
MOD_Plk_1	415	421	PF00069	0.460
MOD_Plk_1	554	560	PF00069	0.486
MOD_Plk_1	59	65	PF00069	0.596
MOD_Plk_1	648	654	PF00069	0.296
MOD_Plk_1	705	711	PF00069	0.357
MOD_Plk_4	104	110	PF00069	0.473
MOD_Plk_4	354	360	PF00069	0.415
MOD_Plk_4	436	442	PF00069	0.367
MOD_Plk_4	471	477	PF00069	0.512
MOD_Plk_4	635	641	PF00069	0.307
MOD_Plk_4	705	711	PF00069	0.298
MOD_ProDKin_1	155	161	PF00069	0.644
MOD_ProDKin_1	303	309	PF00069	0.593
MOD_ProDKin_1	4	10	PF00069	0.537
MOD_ProDKin_1	401	407	PF00069	0.421
MOD_ProDKin_1	448	454	PF00069	0.369
MOD_ProDKin_1	658	664	PF00069	0.297
MOD_SUMO_rev_2	677	686	PF00179	0.357
MOD_SUMO_rev_2	696	703	PF00179	0.357
TRG_DiLeu_BaEn_1	317	322	PF01217	0.399
TRG_DiLeu_BaEn_1	97	102	PF01217	0.530
TRG_DiLeu_BaEn_4	97	103	PF01217	0.395
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.489
TRG_ENDOCYTIC_2	116	119	PF00928	0.436
TRG_ENDOCYTIC_2	320	323	PF00928	0.398
TRG_ENDOCYTIC_2	359	362	PF00928	0.348
TRG_ENDOCYTIC_2	441	444	PF00928	0.354
TRG_ENDOCYTIC_2	657	660	PF00928	0.357
TRG_ENDOCYTIC_2	680	683	PF00928	0.357
TRG_ENDOCYTIC_2	721	724	PF00928	0.287
TRG_ER_diArg_1	13	15	PF00400	0.382
TRG_ER_diArg_1	435	437	PF00400	0.440
TRG_ER_diArg_1	533	536	PF00400	0.503
TRG_NLS_MonoCore_2	219	224	PF00514	0.659
TRG_NLS_MonoExtC_3	219	224	PF00514	0.689
TRG_NLS_MonoExtN_4	219	225	PF00514	0.620
TRG_NLS_MonoExtN_4	533	540	PF00514	0.509
TRG_Pf-PMV_PEXEL_1	27	31	PF00026	0.508

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I443	Leptomonas seymouri	56%	100%
A0A3R7L2R2	Trypanosoma rangeli	36%	100%
A0A3S7X3S1	Leishmania donovani	90%	100%
A4HIK4	Leishmania braziliensis	69%	100%
A4I5U9	Leishmania infantum	90%	100%
E9B144	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%