SAM-dependent MTase TRM10-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA (Guanine-1)-methyltransferase, putative

Species:

Leishmania major

UniProt:

Q4Q6Y3_LEIMA

TriTrypDb:

LmjF.30.3070 , LMJLV39_300039000 , LMJSD75_300038100

Length:

387

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q6Y3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6Y3

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0002939	tRNA N1-guanine methylation	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	8
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	8
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000049	tRNA binding	5	2
GO:0003676	nucleic acid binding	3	2
GO:0003723	RNA binding	4	2
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	2
GO:0008168	methyltransferase activity	4	10
GO:0008173	RNA methyltransferase activity	4	4
GO:0008175	tRNA methyltransferase activity	5	4
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	4
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	4
GO:0016423	tRNA (guanine) methyltransferase activity	6	4
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	10
GO:0097159	organic cyclic compound binding	2	2
GO:0140098	catalytic activity, acting on RNA	3	4
GO:0140101	catalytic activity, acting on a tRNA	4	4
GO:0140640	catalytic activity, acting on a nucleic acid	2	4
GO:1901363	heterocyclic compound binding	2	2
GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity	8	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	185	189	PF00656	0.436
CLV_C14_Caspase3-7	35	39	PF00656	0.737
CLV_NRD_NRD_1	104	106	PF00675	0.549
CLV_NRD_NRD_1	115	117	PF00675	0.416
CLV_NRD_NRD_1	210	212	PF00675	0.272
CLV_NRD_NRD_1	329	331	PF00675	0.539
CLV_NRD_NRD_1	338	340	PF00675	0.592
CLV_NRD_NRD_1	55	57	PF00675	0.628
CLV_NRD_NRD_1	77	79	PF00675	0.496
CLV_PCSK_FUR_1	338	342	PF00082	0.653
CLV_PCSK_FUR_1	53	57	PF00082	0.633
CLV_PCSK_FUR_1	75	79	PF00082	0.514
CLV_PCSK_KEX2_1	104	106	PF00082	0.546
CLV_PCSK_KEX2_1	114	116	PF00082	0.428
CLV_PCSK_KEX2_1	210	212	PF00082	0.272
CLV_PCSK_KEX2_1	329	331	PF00082	0.539
CLV_PCSK_KEX2_1	338	340	PF00082	0.592
CLV_PCSK_KEX2_1	55	57	PF00082	0.592
CLV_PCSK_KEX2_1	63	65	PF00082	0.469
CLV_PCSK_KEX2_1	74	76	PF00082	0.352
CLV_PCSK_KEX2_1	77	79	PF00082	0.421
CLV_PCSK_PC1ET2_1	340	342	PF00082	0.612
CLV_PCSK_PC1ET2_1	63	65	PF00082	0.533
CLV_PCSK_PC1ET2_1	74	76	PF00082	0.559
CLV_PCSK_SKI1_1	293	297	PF00082	0.253
CLV_PCSK_SKI1_1	63	67	PF00082	0.530
DEG_SCF_FBW7_1	13	19	PF00400	0.646
DOC_CKS1_1	13	18	PF01111	0.643
DOC_CKS1_1	45	50	PF01111	0.564
DOC_MAPK_gen_1	101	111	PF00069	0.509
DOC_MAPK_gen_1	336	346	PF00069	0.722
DOC_PP2B_LxvP_1	225	228	PF13499	0.506
DOC_USP7_MATH_1	16	20	PF00917	0.766
DOC_USP7_MATH_1	41	45	PF00917	0.711
DOC_USP7_UBL2_3	336	340	PF12436	0.706
DOC_USP7_UBL2_3	70	74	PF12436	0.509
DOC_WW_Pin1_4	235	240	PF00397	0.547
DOC_WW_Pin1_4	312	317	PF00397	0.538
DOC_WW_Pin1_4	44	49	PF00397	0.678
DOC_WW_Pin1_4	9	14	PF00397	0.740
LIG_14-3-3_CanoR_1	341	347	PF00244	0.679
LIG_14-3-3_CanoR_1	53	59	PF00244	0.578
LIG_Actin_WH2_2	153	170	PF00022	0.538
LIG_Actin_WH2_2	296	313	PF00022	0.515
LIG_BRCT_BRCA1_1	222	226	PF00533	0.538
LIG_CaM_NSCaTE_8	318	325	PF13499	0.506
LIG_EH1_1	298	306	PF00400	0.538
LIG_FHA_1	118	124	PF00498	0.497
LIG_FHA_1	247	253	PF00498	0.480
LIG_FHA_1	275	281	PF00498	0.496
LIG_FHA_2	241	247	PF00498	0.513
LIG_FHA_2	55	61	PF00498	0.566
LIG_FHA_2	95	101	PF00498	0.520
LIG_LIR_Gen_1	163	172	PF02991	0.472
LIG_LIR_Nem_3	163	167	PF02991	0.472
LIG_LIR_Nem_3	207	212	PF02991	0.466
LIG_Pex14_1	222	226	PF04695	0.472
LIG_Pex14_2	140	144	PF04695	0.331
LIG_RPA_C_Fungi	110	122	PF08784	0.586
LIG_SH2_CRK	164	168	PF00017	0.311
LIG_SH2_CRK	262	266	PF00017	0.466
LIG_SH2_NCK_1	267	271	PF00017	0.423
LIG_SH2_STAT5	251	254	PF00017	0.330
LIG_SH3_3	10	16	PF00018	0.738
LIG_SH3_3	130	136	PF00018	0.465
LIG_SH3_3	170	176	PF00018	0.330
LIG_TRAF2_1	59	62	PF00917	0.493
LIG_TRAF2_1	97	100	PF00917	0.510
LIG_TYR_ITIM	162	167	PF00017	0.378
LIG_TYR_ITIM	305	310	PF00017	0.423
MOD_CK1_1	12	18	PF00069	0.717
MOD_CK1_1	155	161	PF00069	0.311
MOD_CK1_1	238	244	PF00069	0.412
MOD_CK1_1	266	272	PF00069	0.455
MOD_CK1_1	44	50	PF00069	0.779
MOD_CK2_1	184	190	PF00069	0.416
MOD_CK2_1	240	246	PF00069	0.336
MOD_CK2_1	362	368	PF00069	0.711
MOD_CK2_1	54	60	PF00069	0.583
MOD_CK2_1	94	100	PF00069	0.549
MOD_Cter_Amidation	336	339	PF01082	0.729
MOD_GlcNHglycan	164	167	PF01048	0.354
MOD_GlcNHglycan	23	26	PF01048	0.665
MOD_GlcNHglycan	268	271	PF01048	0.442
MOD_GlcNHglycan	30	34	PF01048	0.615
MOD_GlcNHglycan	348	351	PF01048	0.675
MOD_GlcNHglycan	374	377	PF01048	0.644
MOD_GSK3_1	12	19	PF00069	0.735
MOD_GSK3_1	146	153	PF00069	0.515
MOD_GSK3_1	162	169	PF00069	0.291
MOD_GSK3_1	21	28	PF00069	0.662
MOD_GSK3_1	29	36	PF00069	0.511
MOD_GSK3_1	342	349	PF00069	0.743
MOD_N-GLC_1	372	377	PF02516	0.720
MOD_N-GLC_2	124	126	PF02516	0.452
MOD_N-GLC_2	290	292	PF02516	0.311
MOD_NEK2_1	140	145	PF00069	0.344
MOD_NEK2_1	162	167	PF00069	0.307
MOD_NEK2_1	17	22	PF00069	0.653
MOD_PIKK_1	155	161	PF00454	0.311
MOD_PIKK_1	214	220	PF00454	0.455
MOD_PIKK_1	226	232	PF00454	0.208
MOD_PIKK_1	25	31	PF00454	0.568
MOD_PIKK_1	41	47	PF00454	0.503
MOD_PKA_2	342	348	PF00069	0.664
MOD_PKA_2	54	60	PF00069	0.631
MOD_PKA_2	94	100	PF00069	0.622
MOD_Plk_1	140	146	PF00069	0.362
MOD_Plk_2-3	357	363	PF00069	0.763
MOD_Plk_4	177	183	PF00069	0.402
MOD_Plk_4	282	288	PF00069	0.455
MOD_ProDKin_1	235	241	PF00069	0.436
MOD_ProDKin_1	312	318	PF00069	0.423
MOD_ProDKin_1	44	50	PF00069	0.675
MOD_ProDKin_1	9	15	PF00069	0.742
MOD_SUMO_rev_2	60	65	PF00179	0.524
MOD_SUMO_rev_2	98	103	PF00179	0.520
TRG_ENDOCYTIC_2	164	167	PF00928	0.311
TRG_ENDOCYTIC_2	206	209	PF00928	0.409
TRG_ENDOCYTIC_2	262	265	PF00928	0.423
TRG_ENDOCYTIC_2	307	310	PF00928	0.384
TRG_ER_diArg_1	114	116	PF00400	0.474
TRG_ER_diArg_1	209	211	PF00400	0.330
TRG_ER_diArg_1	338	341	PF00400	0.700
TRG_ER_diArg_1	53	56	PF00400	0.698
TRG_NLS_Bipartite_1	329	344	PF00514	0.702
TRG_NLS_MonoExtC_3	338	343	PF00514	0.652
TRG_NLS_MonoExtN_4	336	343	PF00514	0.640

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I770	Leptomonas seymouri	71%	100%
A0A0S4JPX3	Bodo saltans	42%	100%
A0A1X0P1F8	Trypanosomatidae	47%	100%
A0A3S5ISH7	Trypanosoma rangeli	50%	100%
A0A3S7X3S5	Leishmania donovani	89%	100%
A4HIP1	Leishmania braziliensis	75%	100%
A4I5Y8	Leishmania infantum	89%	100%
E9B181	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O14214	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
Q08DP1	Bos taurus	27%	100%
Q12400	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
Q4I8X0	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	27%	95%
Q4WXA1	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	98%
Q59Q39	Candida albicans (strain SC5314 / ATCC MYA-2876)	26%	100%
Q5B8X0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	27%	93%
Q5RJK3	Rattus norvegicus	29%	100%
Q6BWG3	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	25%	100%
Q6CUM6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	27%	100%
Q6FQB2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	28%	100%
Q6PF06	Homo sapiens	26%	100%
Q9D075	Mus musculus	24%	100%
V5DNZ8	Trypanosoma cruzi	46%	100%