Putative serine/threonine-protein kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

serine/arginine-rich protein specific kinase SRPK, putative

Species:

Leishmania major

UniProt:

Q4Q6T0_LEIMA

TriTrypDb:

LmjF.30.3580 , LMJLV39_300044600 , LMJSD75_300043700

Length:

747

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q6T0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6T0

Function

Biological processes
Term	Name	Level	Count
GO:0000245	spliceosomal complex assembly	7	2
GO:0006468	protein phosphorylation	5	12
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0007165	signal transduction	2	2
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	2
GO:0016310	phosphorylation	5	12
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019538	protein metabolic process	3	12
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0035556	intracellular signal transduction	3	2
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0050684	regulation of mRNA processing	7	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	12
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1903311	regulation of mRNA metabolic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004672	protein kinase activity	3	12
GO:0004674	protein serine/threonine kinase activity	4	4
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140096	catalytic activity, acting on a protein	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	117	121	PF00656	0.629
CLV_C14_Caspase3-7	369	373	PF00656	0.728
CLV_C14_Caspase3-7	585	589	PF00656	0.634
CLV_NRD_NRD_1	141	143	PF00675	0.385
CLV_NRD_NRD_1	25	27	PF00675	0.615
CLV_NRD_NRD_1	336	338	PF00675	0.472
CLV_NRD_NRD_1	346	348	PF00675	0.526
CLV_PCSK_KEX2_1	25	27	PF00082	0.568
CLV_PCSK_KEX2_1	335	337	PF00082	0.491
CLV_PCSK_KEX2_1	607	609	PF00082	0.709
CLV_PCSK_PC1ET2_1	25	27	PF00082	0.568
CLV_PCSK_PC1ET2_1	607	609	PF00082	0.743
CLV_PCSK_SKI1_1	160	164	PF00082	0.272
CLV_PCSK_SKI1_1	26	30	PF00082	0.654
CLV_PCSK_SKI1_1	271	275	PF00082	0.255
CLV_PCSK_SKI1_1	328	332	PF00082	0.484
CLV_PCSK_SKI1_1	341	345	PF00082	0.439
CLV_PCSK_SKI1_1	348	352	PF00082	0.396
CLV_PCSK_SKI1_1	405	409	PF00082	0.241
CLV_PCSK_SKI1_1	498	502	PF00082	0.257
CLV_PCSK_SKI1_1	521	525	PF00082	0.327
CLV_PCSK_SKI1_1	639	643	PF00082	0.428
CLV_PCSK_SKI1_1	9	13	PF00082	0.713
DEG_APCC_DBOX_1	270	278	PF00400	0.255
DEG_SCF_FBW7_1	296	303	PF00400	0.358
DOC_ANK_TNKS_1	106	113	PF00023	0.614
DOC_CKS1_1	297	302	PF01111	0.332
DOC_CKS1_1	427	432	PF01111	0.234
DOC_MAPK_gen_1	181	189	PF00069	0.299
DOC_MAPK_gen_1	414	423	PF00069	0.241
DOC_MAPK_HePTP_8	411	423	PF00069	0.241
DOC_MAPK_MEF2A_6	414	423	PF00069	0.241
DOC_PP1_RVXF_1	161	168	PF00149	0.241
DOC_PP4_FxxP_1	30	33	PF00568	0.695
DOC_PP4_FxxP_1	311	314	PF00568	0.467
DOC_PP4_FxxP_1	529	532	PF00568	0.241
DOC_USP7_MATH_1	10	14	PF00917	0.495
DOC_USP7_MATH_1	300	304	PF00917	0.451
DOC_USP7_MATH_1	33	37	PF00917	0.663
DOC_USP7_MATH_1	466	470	PF00917	0.388
DOC_USP7_MATH_1	606	610	PF00917	0.740
DOC_USP7_MATH_1	616	620	PF00917	0.600
DOC_USP7_UBL2_3	12	16	PF12436	0.594
DOC_USP7_UBL2_3	23	27	PF12436	0.643
DOC_USP7_UBL2_3	328	332	PF12436	0.471
DOC_USP7_UBL2_3	340	344	PF12436	0.445
DOC_USP7_UBL2_3	5	9	PF12436	0.595
DOC_USP7_UBL2_3	517	521	PF12436	0.253
DOC_WW_Pin1_4	296	301	PF00397	0.287
DOC_WW_Pin1_4	426	431	PF00397	0.244
DOC_WW_Pin1_4	540	545	PF00397	0.408
LIG_14-3-3_CanoR_1	319	327	PF00244	0.642
LIG_14-3-3_CanoR_1	347	351	PF00244	0.668
LIG_14-3-3_CanoR_1	669	678	PF00244	0.523
LIG_APCC_ABBA_1	242	247	PF00400	0.255
LIG_BRCT_BRCA1_1	618	622	PF00533	0.674
LIG_Clathr_ClatBox_1	274	278	PF01394	0.255
LIG_CtBP_PxDLS_1	476	480	PF00389	0.255
LIG_deltaCOP1_diTrp_1	381	389	PF00928	0.553
LIG_deltaCOP1_diTrp_1	432	441	PF00928	0.241
LIG_EH1_1	301	309	PF00400	0.399
LIG_eIF4E_1	201	207	PF01652	0.241
LIG_FHA_1	170	176	PF00498	0.241
LIG_FHA_1	32	38	PF00498	0.553
LIG_FHA_1	406	412	PF00498	0.241
LIG_FHA_1	673	679	PF00498	0.598
LIG_FHA_2	124	130	PF00498	0.581
LIG_FHA_2	131	137	PF00498	0.460
LIG_FHA_2	321	327	PF00498	0.648
LIG_FHA_2	378	384	PF00498	0.522
LIG_FHA_2	427	433	PF00498	0.241
LIG_FHA_2	585	591	PF00498	0.669
LIG_FHA_2	632	638	PF00498	0.522
LIG_FHA_2	640	646	PF00498	0.418
LIG_FHA_2	694	700	PF00498	0.677
LIG_FHA_2	733	739	PF00498	0.615
LIG_Integrin_RGD_1	361	363	PF01839	0.614
LIG_LIR_Apic_2	413	419	PF02991	0.241
LIG_LIR_Apic_2	425	430	PF02991	0.241
LIG_LIR_Apic_2	528	532	PF02991	0.255
LIG_LIR_Gen_1	182	189	PF02991	0.315
LIG_LIR_Gen_1	198	207	PF02991	0.357
LIG_LIR_Gen_1	387	397	PF02991	0.332
LIG_LIR_Nem_3	157	162	PF02991	0.286
LIG_LIR_Nem_3	198	204	PF02991	0.266
LIG_LIR_Nem_3	257	261	PF02991	0.257
LIG_LIR_Nem_3	387	392	PF02991	0.453
LIG_LIR_Nem_3	403	407	PF02991	0.178
LIG_LIR_Nem_3	483	489	PF02991	0.352
LIG_LIR_Nem_3	502	508	PF02991	0.194
LIG_LIR_Nem_3	552	557	PF02991	0.358
LIG_LIR_Nem_3	559	565	PF02991	0.404
LIG_MYND_3	531	535	PF01753	0.234
LIG_Pex14_2	170	174	PF04695	0.264
LIG_PTB_Apo_2	153	160	PF02174	0.393
LIG_PTB_Apo_2	629	636	PF02174	0.570
LIG_PTB_Phospho_1	153	159	PF10480	0.396
LIG_PTB_Phospho_1	629	635	PF10480	0.576
LIG_Rb_pABgroove_1	236	244	PF01858	0.278
LIG_SH2_CRK	159	163	PF00017	0.339
LIG_SH2_CRK	258	262	PF00017	0.241
LIG_SH2_CRK	339	343	PF00017	0.437
LIG_SH2_CRK	427	431	PF00017	0.234
LIG_SH2_CRK	562	566	PF00017	0.516
LIG_SH2_GRB2like	490	493	PF00017	0.321
LIG_SH2_NCK_1	196	200	PF00017	0.255
LIG_SH2_NCK_1	596	600	PF00017	0.686
LIG_SH2_SRC	201	204	PF00017	0.241
LIG_SH2_STAP1	130	134	PF00017	0.529
LIG_SH2_STAP1	196	200	PF00017	0.305
LIG_SH2_STAT5	169	172	PF00017	0.283
LIG_SH2_STAT5	227	230	PF00017	0.272
LIG_SH2_STAT5	635	638	PF00017	0.528
LIG_SUMO_SIM_anti_2	282	289	PF11976	0.255
LIG_SUMO_SIM_anti_2	303	309	PF11976	0.453
LIG_SUMO_SIM_anti_2	461	470	PF11976	0.241
LIG_SUMO_SIM_anti_2	712	718	PF11976	0.500
LIG_SUMO_SIM_par_1	247	254	PF11976	0.241
LIG_SUMO_SIM_par_1	428	435	PF11976	0.234
LIG_TRAF2_1	343	346	PF00917	0.570
LIG_TRAF2_1	580	583	PF00917	0.635
LIG_TRAF2_1	735	738	PF00917	0.580
LIG_TYR_ITIM	503	508	PF00017	0.388
LIG_UBA3_1	304	309	PF00899	0.372
MOD_CDK_SPK_2	296	301	PF00069	0.255
MOD_CK1_1	192	198	PF00069	0.388
MOD_CK1_1	303	309	PF00069	0.449
MOD_CK1_1	35	41	PF00069	0.602
MOD_CK1_1	375	381	PF00069	0.686
MOD_CK1_1	400	406	PF00069	0.241
MOD_CK1_1	589	595	PF00069	0.631
MOD_CK1_1	598	604	PF00069	0.704
MOD_CK1_1	672	678	PF00069	0.569
MOD_CK2_1	105	111	PF00069	0.682
MOD_CK2_1	130	136	PF00069	0.507
MOD_CK2_1	192	198	PF00069	0.388
MOD_CK2_1	248	254	PF00069	0.257
MOD_CK2_1	377	383	PF00069	0.557
MOD_CK2_1	456	462	PF00069	0.241
MOD_CK2_1	569	575	PF00069	0.561
MOD_CK2_1	577	583	PF00069	0.616
MOD_CK2_1	62	68	PF00069	0.443
MOD_CK2_1	631	637	PF00069	0.568
MOD_CK2_1	732	738	PF00069	0.613
MOD_CK2_1	740	746	PF00069	0.555
MOD_CMANNOS	174	177	PF00535	0.241
MOD_GlcNHglycan	12	15	PF01048	0.512
MOD_GlcNHglycan	191	194	PF01048	0.241
MOD_GlcNHglycan	569	574	PF01048	0.612
MOD_GlcNHglycan	575	580	PF01048	0.627
MOD_GlcNHglycan	597	600	PF01048	0.660
MOD_GSK3_1	101	108	PF00069	0.707
MOD_GSK3_1	12	19	PF00069	0.709
MOD_GSK3_1	296	303	PF00069	0.366
MOD_GSK3_1	31	38	PF00069	0.640
MOD_GSK3_1	326	333	PF00069	0.497
MOD_GSK3_1	586	593	PF00069	0.650
MOD_GSK3_1	594	601	PF00069	0.647
MOD_GSK3_1	672	679	PF00069	0.573
MOD_GSK3_1	701	708	PF00069	0.693
MOD_N-GLC_1	233	238	PF02516	0.296
MOD_N-GLC_1	631	636	PF02516	0.568
MOD_N-GLC_2	218	220	PF02516	0.366
MOD_N-GLC_2	399	401	PF02516	0.241
MOD_NEK2_1	1	6	PF00069	0.701
MOD_NEK2_1	228	233	PF00069	0.255
MOD_NEK2_1	330	335	PF00069	0.452
MOD_NEK2_1	397	402	PF00069	0.241
MOD_NEK2_1	631	636	PF00069	0.568
MOD_NEK2_1	81	86	PF00069	0.627
MOD_PIKK_1	330	336	PF00454	0.467
MOD_PIKK_1	590	596	PF00454	0.575
MOD_PIKK_1	702	708	PF00454	0.741
MOD_PKA_1	16	22	PF00069	0.749
MOD_PKA_2	346	352	PF00069	0.668
MOD_PKA_2	41	47	PF00069	0.616
MOD_PKA_2	676	682	PF00069	0.677
MOD_Plk_1	631	637	PF00069	0.514
MOD_Plk_2-3	584	590	PF00069	0.805
MOD_Plk_2-3	732	738	PF00069	0.725
MOD_Plk_2-3	740	746	PF00069	0.699
MOD_Plk_4	169	175	PF00069	0.268
MOD_Plk_4	248	254	PF00069	0.241
MOD_Plk_4	300	306	PF00069	0.418
MOD_Plk_4	346	352	PF00069	0.514
MOD_Plk_4	645	651	PF00069	0.581
MOD_ProDKin_1	296	302	PF00069	0.287
MOD_ProDKin_1	426	432	PF00069	0.244
MOD_ProDKin_1	540	546	PF00069	0.408
MOD_SUMO_for_1	290	293	PF00179	0.241
MOD_SUMO_for_1	343	346	PF00179	0.527
MOD_SUMO_rev_2	197	207	PF00179	0.258
MOD_SUMO_rev_2	284	292	PF00179	0.255
MOD_SUMO_rev_2	324	330	PF00179	0.485
MOD_SUMO_rev_2	345	350	PF00179	0.508
MOD_SUMO_rev_2	353	360	PF00179	0.531
MOD_SUMO_rev_2	55	63	PF00179	0.584
MOD_SUMO_rev_2	597	606	PF00179	0.626
TRG_DiLeu_BaEn_1	666	671	PF01217	0.452
TRG_DiLeu_BaEn_2	524	530	PF01217	0.255
TRG_DiLeu_BaEn_3	345	351	PF01217	0.422
TRG_DiLeu_BaEn_4	626	632	PF01217	0.580
TRG_DiLeu_BaEn_4	673	679	PF01217	0.655
TRG_DiLeu_LyEn_5	666	671	PF01217	0.493
TRG_ENDOCYTIC_2	146	149	PF00928	0.390
TRG_ENDOCYTIC_2	159	162	PF00928	0.313
TRG_ENDOCYTIC_2	184	187	PF00928	0.328
TRG_ENDOCYTIC_2	201	204	PF00928	0.321
TRG_ENDOCYTIC_2	258	261	PF00928	0.241
TRG_ENDOCYTIC_2	339	342	PF00928	0.440
TRG_ENDOCYTIC_2	404	407	PF00928	0.245
TRG_ENDOCYTIC_2	505	508	PF00928	0.388
TRG_ENDOCYTIC_2	562	565	PF00928	0.444
TRG_ER_diArg_1	335	337	PF00400	0.574
TRG_NLS_Bipartite_1	16	30	PF00514	0.659
TRG_NLS_MonoExtC_3	24	29	PF00514	0.647
TRG_NLS_MonoExtC_3	519	524	PF00514	0.296
TRG_NLS_MonoExtN_4	23	30	PF00514	0.653
TRG_NLS_MonoExtN_4	517	524	PF00514	0.296
TRG_NLS_MonoExtN_4	605	611	PF00514	0.536
TRG_Pf-PMV_PEXEL_1	205	209	PF00026	0.255
TRG_Pf-PMV_PEXEL_1	498	502	PF00026	0.255
TRG_Pf-PMV_PEXEL_1	669	673	PF00026	0.516

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1H8	Leptomonas seymouri	81%	100%
A0A0S4JMD1	Bodo saltans	55%	97%
A0A1X0P1B0	Trypanosomatidae	60%	100%
A0A3R7KF70	Trypanosoma rangeli	59%	100%
A0A3S7X404	Leishmania donovani	95%	100%
A4HIU4	Leishmania braziliensis	84%	100%
A4I649	Leishmania infantum	94%	100%
C9ZRG9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9B1D2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5B752	Trypanosoma cruzi	64%	100%