Bifunctional lysine-specific demethylase and histidyl-hydroxylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cupin superfamily protein, putative

Species:

Leishmania major

UniProt:

Q4Q6P0_LEIMA

TriTrypDb:

LmjF.31.0240 , LMJLV39_310007600 , LMJSD75_310007600

Length:

624

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	9
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	9
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	9
GO:0005634	nucleus	5	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7

Expansion

Sequence features

Q4Q6P0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6P0

Function

Biological processes
Term	Name	Level	Count
GO:0006482	protein demethylation	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	3
GO:0008214	protein dealkylation	5	2
GO:0016570	histone modification	5	2
GO:0016577	histone demethylation	4	2
GO:0019538	protein metabolic process	3	2
GO:0034720	histone H3-K4 demethylation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070076	histone lysine demethylation	5	2
GO:0070544	histone H3-K36 demethylation	6	2
GO:0070988	demethylation	2	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:0032259	methylation	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005506	iron ion binding	6	9
GO:0016491	oxidoreductase activity	2	9
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	9
GO:0016706	2-oxoglutarate-dependent dioxygenase activity	4	9
GO:0032451	demethylase activity	2	2
GO:0032452	histone demethylase activity	4	2
GO:0032453	histone H3K4 demethylase activity	5	2
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046914	transition metal ion binding	5	9
GO:0051213	dioxygenase activity	3	9
GO:0051864	histone H3K36 demethylase activity	5	2
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0140457	protein demethylase activity	3	2
GO:0008168	methyltransferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016741	transferase activity, transferring one-carbon groups	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	605	609	PF00656	0.415
CLV_MEL_PAP_1	240	246	PF00089	0.448
CLV_NRD_NRD_1	142	144	PF00675	0.554
CLV_NRD_NRD_1	17	19	PF00675	0.478
CLV_NRD_NRD_1	179	181	PF00675	0.425
CLV_NRD_NRD_1	184	186	PF00675	0.521
CLV_NRD_NRD_1	242	244	PF00675	0.562
CLV_NRD_NRD_1	422	424	PF00675	0.383
CLV_NRD_NRD_1	511	513	PF00675	0.360
CLV_PCSK_KEX2_1	130	132	PF00082	0.692
CLV_PCSK_KEX2_1	17	19	PF00082	0.448
CLV_PCSK_KEX2_1	241	243	PF00082	0.389
CLV_PCSK_KEX2_1	422	424	PF00082	0.414
CLV_PCSK_PC1ET2_1	130	132	PF00082	0.692
CLV_PCSK_PC1ET2_1	241	243	PF00082	0.389
CLV_PCSK_SKI1_1	143	147	PF00082	0.611
CLV_PCSK_SKI1_1	422	426	PF00082	0.472
CLV_PCSK_SKI1_1	529	533	PF00082	0.342
DEG_Nend_Nbox_1	1	3	PF02207	0.453
DEG_SCF_FBW7_1	354	359	PF00400	0.309
DEG_SPOP_SBC_1	76	80	PF00917	0.445
DOC_CKS1_1	299	304	PF01111	0.267
DOC_CKS1_1	353	358	PF01111	0.343
DOC_MAPK_gen_1	29	37	PF00069	0.453
DOC_MAPK_HePTP_8	522	534	PF00069	0.417
DOC_MAPK_MEF2A_6	383	391	PF00069	0.303
DOC_MAPK_MEF2A_6	525	534	PF00069	0.421
DOC_MAPK_NFAT4_5	525	533	PF00069	0.406
DOC_PP1_RVXF_1	416	422	PF00149	0.405
DOC_PP2B_LxvP_1	368	371	PF13499	0.299
DOC_PP2B_LxvP_1	497	500	PF13499	0.422
DOC_PP2B_LxvP_1	532	535	PF13499	0.464
DOC_PP4_FxxP_1	269	272	PF00568	0.303
DOC_PP4_FxxP_1	353	356	PF00568	0.338
DOC_USP7_MATH_1	154	158	PF00917	0.611
DOC_USP7_MATH_1	302	306	PF00917	0.267
DOC_USP7_MATH_1	31	35	PF00917	0.543
DOC_USP7_MATH_1	341	345	PF00917	0.294
DOC_USP7_UBL2_3	235	239	PF12436	0.428
DOC_USP7_UBL2_3	379	383	PF12436	0.343
DOC_WW_Pin1_4	107	112	PF00397	0.548
DOC_WW_Pin1_4	27	32	PF00397	0.517
DOC_WW_Pin1_4	298	303	PF00397	0.267
DOC_WW_Pin1_4	352	357	PF00397	0.343
DOC_WW_Pin1_4	78	83	PF00397	0.475
LIG_14-3-3_CanoR_1	242	247	PF00244	0.557
LIG_14-3-3_CanoR_1	32	36	PF00244	0.539
LIG_14-3-3_CanoR_1	342	350	PF00244	0.285
LIG_14-3-3_CanoR_1	512	520	PF00244	0.431
LIG_14-3-3_CanoR_1	96	103	PF00244	0.470
LIG_APCC_ABBA_1	308	313	PF00400	0.267
LIG_BRCT_BRCA1_1	265	269	PF00533	0.298
LIG_BRCT_BRCA1_1	304	308	PF00533	0.267
LIG_BRCT_BRCA1_1	33	37	PF00533	0.455
LIG_Clathr_ClatBox_1	600	604	PF01394	0.426
LIG_deltaCOP1_diTrp_1	263	269	PF00928	0.299
LIG_DLG_GKlike_1	133	141	PF00625	0.504
LIG_eIF4E_1	246	252	PF01652	0.365
LIG_FHA_1	246	252	PF00498	0.437
LIG_FHA_1	357	363	PF00498	0.381
LIG_FHA_1	382	388	PF00498	0.367
LIG_FHA_1	442	448	PF00498	0.483
LIG_FHA_1	471	477	PF00498	0.440
LIG_FHA_1	546	552	PF00498	0.375
LIG_FHA_1	576	582	PF00498	0.443
LIG_FHA_1	78	84	PF00498	0.438
LIG_FHA_2	603	609	PF00498	0.373
LIG_FHA_2	97	103	PF00498	0.468
LIG_LIR_Apic_2	266	272	PF02991	0.300
LIG_LIR_Apic_2	298	302	PF02991	0.267
LIG_LIR_Apic_2	351	356	PF02991	0.338
LIG_LIR_Gen_1	316	327	PF02991	0.245
LIG_LIR_Gen_1	398	407	PF02991	0.344
LIG_LIR_Gen_1	56	63	PF02991	0.425
LIG_LIR_LC3C_4	56	61	PF02991	0.425
LIG_LIR_Nem_3	177	182	PF02991	0.387
LIG_LIR_Nem_3	287	293	PF02991	0.340
LIG_LIR_Nem_3	316	322	PF02991	0.355
LIG_LIR_Nem_3	325	331	PF02991	0.279
LIG_LIR_Nem_3	398	402	PF02991	0.345
LIG_LIR_Nem_3	56	61	PF02991	0.425
LIG_MLH1_MIPbox_1	33	37	PF16413	0.455
LIG_NRBOX	471	477	PF00104	0.358
LIG_PCNA_yPIPBox_3	458	469	PF02747	0.403
LIG_Pex14_1	265	269	PF04695	0.298
LIG_Pex14_2	179	183	PF04695	0.350
LIG_Pex14_2	560	564	PF04695	0.326
LIG_PTB_Apo_2	435	442	PF02174	0.417
LIG_Rb_LxCxE_1	278	298	PF01857	0.351
LIG_Rb_pABgroove_1	325	333	PF01858	0.343
LIG_REV1ctd_RIR_1	176	185	PF16727	0.357
LIG_SH2_NCK_1	312	316	PF00017	0.267
LIG_SH2_NCK_1	331	335	PF00017	0.267
LIG_SH2_SRC	312	315	PF00017	0.267
LIG_SH2_SRC	331	334	PF00017	0.301
LIG_SH2_STAT3	67	70	PF00017	0.428
LIG_SH2_STAT5	182	185	PF00017	0.344
LIG_SH2_STAT5	197	200	PF00017	0.382
LIG_SH2_STAT5	293	296	PF00017	0.389
LIG_SH2_STAT5	36	39	PF00017	0.483
LIG_SH2_STAT5	409	412	PF00017	0.398
LIG_SH2_STAT5	58	61	PF00017	0.451
LIG_SH3_3	202	208	PF00018	0.452
LIG_SH3_3	433	439	PF00018	0.397
LIG_SH3_3	79	85	PF00018	0.518
LIG_SUMO_SIM_anti_2	230	235	PF11976	0.343
LIG_SUMO_SIM_anti_2	335	341	PF11976	0.301
LIG_SUMO_SIM_anti_2	471	479	PF11976	0.383
LIG_SUMO_SIM_par_1	471	479	PF11976	0.443
LIG_SUMO_SIM_par_1	59	64	PF11976	0.425
LIG_SUMO_SIM_par_1	599	605	PF11976	0.424
LIG_TRAF2_1	136	139	PF00917	0.503
LIG_WW_3	14	18	PF00397	0.439
MOD_CDK_SPK_2	27	32	PF00069	0.451
MOD_CDK_SPK_2	352	357	PF00069	0.294
MOD_CDK_SPxxK_3	107	114	PF00069	0.500
MOD_CK1_1	157	163	PF00069	0.631
MOD_CK1_1	191	197	PF00069	0.485
MOD_CK1_1	245	251	PF00069	0.571
MOD_CK1_1	298	304	PF00069	0.267
MOD_CK1_1	30	36	PF00069	0.507
MOD_CK1_1	515	521	PF00069	0.495
MOD_CK2_1	133	139	PF00069	0.774
MOD_CK2_1	171	177	PF00069	0.357
MOD_CK2_1	96	102	PF00069	0.496
MOD_GlcNHglycan	126	129	PF01048	0.609
MOD_GlcNHglycan	193	196	PF01048	0.500
MOD_GlcNHglycan	199	202	PF01048	0.466
MOD_GlcNHglycan	279	282	PF01048	0.291
MOD_GlcNHglycan	391	394	PF01048	0.267
MOD_GlcNHglycan	450	453	PF01048	0.332
MOD_GlcNHglycan	504	507	PF01048	0.494
MOD_GlcNHglycan	581	584	PF01048	0.458
MOD_GlcNHglycan	616	619	PF01048	0.597
MOD_GSK3_1	105	112	PF00069	0.606
MOD_GSK3_1	115	122	PF00069	0.583
MOD_GSK3_1	27	34	PF00069	0.592
MOD_GSK3_1	298	305	PF00069	0.267
MOD_GSK3_1	352	359	PF00069	0.355
MOD_GSK3_1	377	384	PF00069	0.343
MOD_GSK3_1	470	477	PF00069	0.410
MOD_GSK3_1	498	505	PF00069	0.461
MOD_GSK3_1	508	515	PF00069	0.454
MOD_GSK3_1	567	574	PF00069	0.358
MOD_GSK3_1	575	582	PF00069	0.365
MOD_GSK3_1	84	91	PF00069	0.500
MOD_GSK3_1	92	99	PF00069	0.447
MOD_N-GLC_1	171	176	PF02516	0.284
MOD_N-GLC_1	209	214	PF02516	0.375
MOD_N-GLC_1	545	550	PF02516	0.449
MOD_NEK2_1	170	175	PF00069	0.324
MOD_NEK2_1	188	193	PF00069	0.459
MOD_NEK2_1	275	280	PF00069	0.524
MOD_NEK2_1	387	392	PF00069	0.299
MOD_NEK2_1	441	446	PF00069	0.474
MOD_NEK2_1	571	576	PF00069	0.389
MOD_NEK2_1	602	607	PF00069	0.346
MOD_NEK2_1	88	93	PF00069	0.452
MOD_NEK2_2	31	36	PF00069	0.454
MOD_NEK2_2	371	376	PF00069	0.357
MOD_NEK2_2	378	383	PF00069	0.301
MOD_PIKK_1	105	111	PF00454	0.500
MOD_PIKK_1	157	163	PF00454	0.631
MOD_PIKK_1	302	308	PF00454	0.267
MOD_PKA_1	124	130	PF00069	0.636
MOD_PKA_1	242	248	PF00069	0.571
MOD_PKA_1	512	518	PF00069	0.304
MOD_PKA_2	16	22	PF00069	0.450
MOD_PKA_2	242	248	PF00069	0.571
MOD_PKA_2	31	37	PF00069	0.486
MOD_PKA_2	341	347	PF00069	0.290
MOD_PKA_2	371	377	PF00069	0.341
MOD_PKA_2	441	447	PF00069	0.491
MOD_PKB_1	131	139	PF00069	0.604
MOD_PKB_1	519	527	PF00069	0.394
MOD_PKB_1	94	102	PF00069	0.443
MOD_Plk_1	133	139	PF00069	0.604
MOD_Plk_1	470	476	PF00069	0.384
MOD_Plk_1	545	551	PF00069	0.419
MOD_Plk_4	295	301	PF00069	0.269
MOD_Plk_4	31	37	PF00069	0.521
MOD_Plk_4	470	476	PF00069	0.406
MOD_Plk_4	567	573	PF00069	0.323
MOD_Plk_4	88	94	PF00069	0.466
MOD_ProDKin_1	107	113	PF00069	0.546
MOD_ProDKin_1	27	33	PF00069	0.517
MOD_ProDKin_1	298	304	PF00069	0.267
MOD_ProDKin_1	352	358	PF00069	0.343
MOD_ProDKin_1	78	84	PF00069	0.475
MOD_SUMO_for_1	145	148	PF00179	0.632
MOD_SUMO_rev_2	232	240	PF00179	0.366
TRG_DiLeu_BaEn_1	184	189	PF01217	0.413
TRG_DiLeu_BaEn_1	471	476	PF01217	0.378
TRG_DiLeu_BaEn_1	597	602	PF01217	0.437
TRG_DiLeu_BaLyEn_6	518	523	PF01217	0.451
TRG_DiLeu_BaLyEn_6	526	531	PF01217	0.361
TRG_ENDOCYTIC_2	312	315	PF00928	0.252
TRG_ENDOCYTIC_2	58	61	PF00928	0.451
TRG_ER_diArg_1	16	18	PF00400	0.485
TRG_ER_diArg_1	242	244	PF00400	0.381
TRG_ER_diArg_1	421	423	PF00400	0.389
TRG_ER_diArg_1	519	522	PF00400	0.383
TRG_ER_diArg_1	93	96	PF00400	0.450
TRG_NES_CRM1_1	471	483	PF08389	0.304
TRG_NLS_Bipartite_1	130	147	PF00514	0.609
TRG_NLS_MonoExtC_3	142	148	PF00514	0.546
TRG_NLS_MonoExtN_4	238	245	PF00514	0.456
TRG_Pf-PMV_PEXEL_1	423	428	PF00026	0.424

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDT4	Leptomonas seymouri	70%	100%
A0A1X0NJI1	Trypanosomatidae	50%	100%
A0A3S7X447	Leishmania donovani	93%	100%
A3KP59	Danio rerio	28%	100%
A4HIZ9	Leishmania braziliensis	79%	100%
A4I6B6	Leishmania infantum	93%	100%
A5PK74	Bos taurus	30%	94%
A8QFQ3	Brugia malayi	25%	88%
A8XEA2	Caenorhabditis briggsae	24%	80%
B0WMG3	Culex quinquefasciatus	30%	96%
B3MSI4	Drosophila ananassae	30%	74%
B3NU20	Drosophila erecta	28%	95%
B4I100	Drosophila sechellia	30%	95%
B4L6Q5	Drosophila mojavensis	30%	70%
B4M7P8	Drosophila virilis	28%	69%
B4NP88	Drosophila willistoni	30%	81%
B4Q068	Drosophila yakuba	28%	91%
B4R4H1	Drosophila simulans	30%	74%
C3XRY1	Branchiostoma floridae	27%	100%
D3ZU57	Rattus norvegicus	31%	100%
E9B1H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q16W06	Aedes aegypti	28%	98%
Q54K96	Dictyostelium discoideum	30%	100%
Q5ZMM1	Gallus gallus	29%	100%
Q7K4H4	Drosophila melanogaster	29%	96%
Q9H6W3	Homo sapiens	30%	97%
Q9JJF3	Mus musculus	31%	100%
V5AYK7	Trypanosoma cruzi	53%	100%