tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

CCCH zinc finger in TRM13 protein/U11-48K-like CHHC zinc finger/Methyltransferase TRM13, putative

Species:

Leishmania major

UniProt:

Q4Q6L1_LEIMA

TriTrypDb:

LmjF.31.0520 , LMJLV39_310010700 , LMJSD75_310010400

Length:

626

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4Q6L1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6L1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0062105	RNA 2'-O-methyltransferase activity	5	12
GO:0106050	tRNA 2'-O-methyltransferase activity	6	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	231	233	PF00675	0.473
CLV_NRD_NRD_1	41	43	PF00675	0.501
CLV_NRD_NRD_1	419	421	PF00675	0.217
CLV_NRD_NRD_1	49	51	PF00675	0.454
CLV_NRD_NRD_1	577	579	PF00675	0.277
CLV_NRD_NRD_1	581	583	PF00675	0.277
CLV_NRD_NRD_1	76	78	PF00675	0.611
CLV_NRD_NRD_1	99	101	PF00675	0.237
CLV_PCSK_FUR_1	47	51	PF00082	0.465
CLV_PCSK_KEX2_1	41	43	PF00082	0.509
CLV_PCSK_KEX2_1	419	421	PF00082	0.210
CLV_PCSK_KEX2_1	47	49	PF00082	0.482
CLV_PCSK_KEX2_1	577	579	PF00082	0.223
CLV_PCSK_KEX2_1	585	587	PF00082	0.195
CLV_PCSK_PC1ET2_1	585	587	PF00082	0.214
CLV_PCSK_SKI1_1	133	137	PF00082	0.578
CLV_PCSK_SKI1_1	171	175	PF00082	0.251
CLV_PCSK_SKI1_1	180	184	PF00082	0.378
CLV_PCSK_SKI1_1	378	382	PF00082	0.225
CLV_PCSK_SKI1_1	41	45	PF00082	0.519
CLV_PCSK_SKI1_1	447	451	PF00082	0.256
CLV_PCSK_SKI1_1	50	54	PF00082	0.495
CLV_PCSK_SKI1_1	611	615	PF00082	0.234
DEG_APCC_DBOX_1	406	414	PF00400	0.480
DEG_APCC_DBOX_1	446	454	PF00400	0.546
DEG_APCC_DBOX_1	49	57	PF00400	0.452
DEG_APCC_DBOX_1	523	531	PF00400	0.434
DEG_Nend_UBRbox_1	1	4	PF02207	0.392
DOC_CKS1_1	30	35	PF01111	0.437
DOC_MAPK_DCC_7	48	58	PF00069	0.451
DOC_MAPK_gen_1	425	433	PF00069	0.427
DOC_MAPK_gen_1	47	55	PF00069	0.490
DOC_MAPK_gen_1	57	65	PF00069	0.437
DOC_MAPK_gen_1	582	593	PF00069	0.431
DOC_MAPK_HePTP_8	46	58	PF00069	0.477
DOC_MAPK_MEF2A_6	407	415	PF00069	0.459
DOC_MAPK_MEF2A_6	49	58	PF00069	0.481
DOC_MAPK_MEF2A_6	586	595	PF00069	0.514
DOC_PP1_RVXF_1	590	596	PF00149	0.514
DOC_PP2B_LxvP_1	543	546	PF13499	0.450
DOC_PP4_FxxP_1	206	209	PF00568	0.527
DOC_USP7_MATH_1	126	130	PF00917	0.587
DOC_USP7_MATH_1	157	161	PF00917	0.534
DOC_USP7_MATH_1	271	275	PF00917	0.661
DOC_USP7_MATH_1	392	396	PF00917	0.441
DOC_USP7_MATH_1	467	471	PF00917	0.506
DOC_USP7_MATH_1	546	550	PF00917	0.494
DOC_USP7_MATH_1	559	563	PF00917	0.363
DOC_USP7_MATH_1	76	80	PF00917	0.721
DOC_WW_Pin1_4	138	143	PF00397	0.655
DOC_WW_Pin1_4	16	21	PF00397	0.457
DOC_WW_Pin1_4	265	270	PF00397	0.736
DOC_WW_Pin1_4	29	34	PF00397	0.464
DOC_WW_Pin1_4	382	387	PF00397	0.456
DOC_WW_Pin1_4	498	503	PF00397	0.427
LIG_14-3-3_CanoR_1	103	111	PF00244	0.454
LIG_14-3-3_CanoR_1	180	189	PF00244	0.514
LIG_14-3-3_CanoR_1	216	222	PF00244	0.371
LIG_14-3-3_CanoR_1	319	327	PF00244	0.446
LIG_14-3-3_CanoR_1	41	47	PF00244	0.488
LIG_14-3-3_CanoR_1	419	426	PF00244	0.493
LIG_14-3-3_CanoR_1	427	433	PF00244	0.459
LIG_14-3-3_CanoR_1	447	457	PF00244	0.355
LIG_14-3-3_CanoR_1	57	62	PF00244	0.432
LIG_14-3-3_CanoR_1	77	85	PF00244	0.462
LIG_Actin_WH2_2	166	182	PF00022	0.477
LIG_BRCT_BRCA1_1	18	22	PF00533	0.427
LIG_BRCT_BRCA1_1	202	206	PF00533	0.339
LIG_BRCT_BRCA1_1	9	13	PF00533	0.445
LIG_Clathr_ClatBox_1	410	414	PF01394	0.452
LIG_FHA_1	116	122	PF00498	0.464
LIG_FHA_1	181	187	PF00498	0.454
LIG_FHA_1	310	316	PF00498	0.522
LIG_FHA_1	32	38	PF00498	0.492
LIG_FHA_1	359	365	PF00498	0.515
LIG_FHA_1	382	388	PF00498	0.542
LIG_FHA_1	608	614	PF00498	0.508
LIG_FHA_2	216	222	PF00498	0.406
LIG_FHA_2	549	555	PF00498	0.503
LIG_FHA_2	607	613	PF00498	0.477
LIG_HP1_1	409	413	PF01393	0.514
LIG_Integrin_RGD_1	149	151	PF01839	0.489
LIG_LIR_Apic_2	203	209	PF02991	0.370
LIG_LIR_Gen_1	330	337	PF02991	0.349
LIG_LIR_Gen_1	477	486	PF02991	0.423
LIG_LIR_Gen_1	554	563	PF02991	0.410
LIG_LIR_LC3C_4	245	248	PF02991	0.348
LIG_LIR_Nem_3	10	16	PF02991	0.445
LIG_LIR_Nem_3	330	335	PF02991	0.376
LIG_LIR_Nem_3	477	482	PF02991	0.436
LIG_LIR_Nem_3	554	560	PF02991	0.424
LIG_LIR_Nem_3	90	96	PF02991	0.362
LIG_Pex14_1	396	400	PF04695	0.425
LIG_SH2_STAP1	165	169	PF00017	0.410
LIG_SH2_STAP1	238	242	PF00017	0.445
LIG_SH2_STAT5	115	118	PF00017	0.469
LIG_SH2_STAT5	181	184	PF00017	0.434
LIG_SH2_STAT5	188	191	PF00017	0.346
LIG_SH2_STAT5	598	601	PF00017	0.444
LIG_SH2_STAT5	608	611	PF00017	0.431
LIG_SH2_STAT5	97	100	PF00017	0.434
LIG_SH3_3	149	155	PF00018	0.436
LIG_SH3_3	27	33	PF00018	0.444
LIG_SH3_3	383	389	PF00018	0.547
LIG_SH3_3	507	513	PF00018	0.497
LIG_SH3_3	527	533	PF00018	0.324
LIG_SH3_3	79	85	PF00018	0.693
LIG_SUMO_SIM_anti_2	245	250	PF11976	0.487
LIG_SUMO_SIM_par_1	408	414	PF11976	0.423
LIG_SUMO_SIM_par_1	513	519	PF11976	0.505
LIG_TRAF2_1	282	285	PF00917	0.568
LIG_TRAF2_1	553	556	PF00917	0.501
MOD_CK1_1	140	146	PF00069	0.580
MOD_CK1_1	160	166	PF00069	0.456
MOD_CK1_1	395	401	PF00069	0.536
MOD_CK1_1	496	502	PF00069	0.421
MOD_CK1_1	562	568	PF00069	0.410
MOD_CK2_1	164	170	PF00069	0.490
MOD_CK2_1	360	366	PF00069	0.521
MOD_CK2_1	373	379	PF00069	0.555
MOD_CK2_1	548	554	PF00069	0.423
MOD_CK2_1	77	83	PF00069	0.714
MOD_Cter_Amidation	580	583	PF01082	0.256
MOD_GlcNHglycan	203	206	PF01048	0.478
MOD_GlcNHglycan	221	225	PF01048	0.469
MOD_GlcNHglycan	259	262	PF01048	0.619
MOD_GlcNHglycan	277	280	PF01048	0.467
MOD_GlcNHglycan	342	345	PF01048	0.325
MOD_GlcNHglycan	428	431	PF01048	0.301
MOD_GSK3_1	136	143	PF00069	0.721
MOD_GSK3_1	156	163	PF00069	0.478
MOD_GSK3_1	211	218	PF00069	0.520
MOD_GSK3_1	265	272	PF00069	0.669
MOD_GSK3_1	369	376	PF00069	0.479
MOD_GSK3_1	378	385	PF00069	0.515
MOD_GSK3_1	387	394	PF00069	0.547
MOD_GSK3_1	498	505	PF00069	0.422
MOD_GSK3_1	5	12	PF00069	0.414
MOD_GSK3_1	546	553	PF00069	0.458
MOD_GSK3_1	57	64	PF00069	0.513
MOD_GSK3_1	607	614	PF00069	0.470
MOD_GSK3_1	92	99	PF00069	0.485
MOD_N-GLC_1	160	165	PF02516	0.210
MOD_N-GLC_2	617	619	PF02516	0.221
MOD_N-GLC_2	92	94	PF02516	0.209
MOD_NEK2_1	201	206	PF00069	0.457
MOD_NEK2_1	493	498	PF00069	0.419
MOD_NEK2_1	503	508	PF00069	0.407
MOD_NEK2_1	61	66	PF00069	0.639
MOD_NEK2_1	9	14	PF00069	0.443
MOD_NEK2_2	271	276	PF00069	0.551
MOD_OFUCOSY	490	497	PF10250	0.234
MOD_PIKK_1	211	217	PF00454	0.573
MOD_PIKK_1	31	37	PF00454	0.494
MOD_PIKK_1	319	325	PF00454	0.536
MOD_PIKK_1	503	509	PF00454	0.456
MOD_PKA_1	419	425	PF00069	0.425
MOD_PKA_1	77	83	PF00069	0.593
MOD_PKA_2	215	221	PF00069	0.408
MOD_PKA_2	275	281	PF00069	0.703
MOD_PKA_2	318	324	PF00069	0.454
MOD_PKA_2	419	425	PF00069	0.454
MOD_PKA_2	426	432	PF00069	0.417
MOD_PKA_2	76	82	PF00069	0.678
MOD_PKB_1	57	65	PF00069	0.464
MOD_Plk_1	160	166	PF00069	0.410
MOD_Plk_1	284	290	PF00069	0.635
MOD_Plk_4	110	116	PF00069	0.494
MOD_Plk_4	160	166	PF00069	0.410
MOD_Plk_4	311	317	PF00069	0.567
MOD_Plk_4	92	98	PF00069	0.514
MOD_ProDKin_1	138	144	PF00069	0.653
MOD_ProDKin_1	16	22	PF00069	0.461
MOD_ProDKin_1	265	271	PF00069	0.736
MOD_ProDKin_1	29	35	PF00069	0.465
MOD_ProDKin_1	382	388	PF00069	0.456
MOD_ProDKin_1	498	504	PF00069	0.427
TRG_DiLeu_BaEn_3	615	621	PF01217	0.418
TRG_DiLeu_BaEn_4	284	290	PF01217	0.543
TRG_DiLeu_BaLyEn_6	499	504	PF01217	0.425
TRG_ENDOCYTIC_2	601	604	PF00928	0.514
TRG_ER_diArg_1	41	43	PF00400	0.501
TRG_ER_diArg_1	418	420	PF00400	0.410
TRG_ER_diArg_1	435	438	PF00400	0.410
TRG_ER_diArg_1	46	49	PF00400	0.504
TRG_NLS_MonoExtC_3	581	586	PF00514	0.456
TRG_Pf-PMV_PEXEL_1	300	304	PF00026	0.462
TRG_Pf-PMV_PEXEL_1	586	590	PF00026	0.308

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMP0	Leptomonas seymouri	56%	100%
A0A0S4IU97	Bodo saltans	34%	100%
A0A1X0NKZ4	Trypanosomatidae	36%	100%
A0A3Q8IFC1	Leishmania donovani	91%	100%
A0A3R7N0X9	Trypanosoma rangeli	40%	100%
A4HJ30	Leishmania braziliensis	75%	89%
A4I6K9	Leishmania infantum	91%	100%
A7TSF4	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	22%	100%
C9ZN61	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9B1K2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q12383	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	21%	100%
V5BII3	Trypanosoma cruzi	40%	100%