GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania major

UniProt:

Q4Q6F2_LEIMA

TriTrypDb:

LmjF.31.1110 , LMJLV39_310017500 , LMJSD75_310017100

Length:

215

Annotations

LeishMANIAdb annotations

Related to other eukaryotic COPII adaptors. Likely involved in ER to Golgi transport.. A Leishmaniid-specific subgroup of these proteins is missing the SigP (unclear topology and function). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 13, no: 7
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	3
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	3
GO:0005794	Golgi apparatus	5	3
GO:0016020	membrane	2	21
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	3
GO:0030135	coated vesicle	7	3
GO:0031410	cytoplasmic vesicle	6	3
GO:0031982	vesicle	4	3
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0097708	intracellular vesicle	5	3
GO:0110165	cellular anatomical entity	1	21

Expansion

Sequence features

Q4Q6F2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6F2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	3
GO:0006886	intracellular protein transport	4	3
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	3
GO:0006996	organelle organization	4	3
GO:0007030	Golgi organization	5	3
GO:0008104	protein localization	4	3
GO:0009987	cellular process	1	3
GO:0015031	protein transport	4	3
GO:0016043	cellular component organization	3	3
GO:0016192	vesicle-mediated transport	4	3
GO:0033036	macromolecule localization	2	3
GO:0045184	establishment of protein localization	3	3
GO:0046907	intracellular transport	3	3
GO:0048193	Golgi vesicle transport	5	3
GO:0051179	localization	1	3
GO:0051234	establishment of localization	2	3
GO:0051641	cellular localization	2	3
GO:0051649	establishment of localization in cell	3	3
GO:0070727	cellular macromolecule localization	3	3
GO:0071702	organic substance transport	4	3
GO:0071705	nitrogen compound transport	4	3
GO:0071840	cellular component organization or biogenesis	2	3

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	206	208	PF00675	0.264
CLV_PCSK_KEX2_1	206	208	PF00082	0.255
CLV_PCSK_SKI1_1	182	186	PF00082	0.398
CLV_PCSK_SKI1_1	206	210	PF00082	0.206
DEG_APCC_DBOX_1	181	189	PF00400	0.171
DEG_Nend_UBRbox_1	1	4	PF02207	0.691
DOC_CYCLIN_RxL_1	203	213	PF00134	0.297
DOC_MAPK_gen_1	102	110	PF00069	0.312
DOC_MAPK_MEF2A_6	102	110	PF00069	0.203
DOC_MAPK_MEF2A_6	18	25	PF00069	0.492
DOC_PP1_RVXF_1	118	125	PF00149	0.227
DOC_PP1_RVXF_1	180	187	PF00149	0.253
DOC_PP1_RVXF_1	55	62	PF00149	0.291
DOC_PP1_RVXF_1	86	93	PF00149	0.358
DOC_PP4_FxxP_1	95	98	PF00568	0.333
LIG_14-3-3_CanoR_1	127	131	PF00244	0.318
LIG_APCC_ABBA_1	63	68	PF00400	0.301
LIG_BRCT_BRCA1_1	25	29	PF00533	0.586
LIG_FHA_1	158	164	PF00498	0.258
LIG_FHA_1	36	42	PF00498	0.299
LIG_FHA_1	48	54	PF00498	0.270
LIG_FHA_2	72	78	PF00498	0.258
LIG_LIR_Apic_2	68	72	PF02991	0.342
LIG_LIR_Gen_1	187	197	PF02991	0.311
LIG_LIR_Gen_1	199	208	PF02991	0.338
LIG_LIR_Gen_1	26	37	PF02991	0.449
LIG_LIR_Nem_3	187	192	PF02991	0.303
LIG_LIR_Nem_3	199	203	PF02991	0.289
LIG_LIR_Nem_3	26	32	PF02991	0.447
LIG_LIR_Nem_3	58	63	PF02991	0.210
LIG_LIR_Nem_3	89	95	PF02991	0.289
LIG_NRBOX	204	210	PF00104	0.469
LIG_PDZ_Class_1	210	215	PF00595	0.534
LIG_SH2_STAP1	134	138	PF00017	0.276
LIG_SH2_STAT5	60	63	PF00017	0.294
LIG_SH2_STAT5	69	72	PF00017	0.322
LIG_Sin3_3	29	36	PF02671	0.431
LIG_SUMO_SIM_par_1	194	199	PF11976	0.378
LIG_SUMO_SIM_par_1	71	77	PF11976	0.274
LIG_TRFH_1	63	67	PF08558	0.301
LIG_UBA3_1	204	212	PF00899	0.468
LIG_WRC_WIRS_1	107	112	PF05994	0.339
LIG_WRC_WIRS_1	197	202	PF05994	0.341
MOD_CK2_1	175	181	PF00069	0.269
MOD_GlcNHglycan	151	154	PF01048	0.521
MOD_GlcNHglycan	177	180	PF01048	0.505
MOD_GlcNHglycan	25	28	PF01048	0.649
MOD_GlcNHglycan	29	32	PF01048	0.667
MOD_GlcNHglycan	33	36	PF01048	0.669
MOD_GSK3_1	106	113	PF00069	0.313
MOD_GSK3_1	157	164	PF00069	0.297
MOD_GSK3_1	23	30	PF00069	0.425
MOD_GSK3_1	31	38	PF00069	0.464
MOD_NEK2_1	1	6	PF00069	0.695
MOD_NEK2_1	110	115	PF00069	0.273
MOD_NEK2_1	116	121	PF00069	0.276
MOD_NEK2_1	126	131	PF00069	0.275
MOD_NEK2_1	151	156	PF00069	0.282
MOD_NEK2_1	161	166	PF00069	0.228
MOD_NEK2_1	184	189	PF00069	0.293
MOD_NEK2_1	23	28	PF00069	0.450
MOD_PKA_2	126	132	PF00069	0.312
MOD_PKA_2	172	178	PF00069	0.243
MOD_PKA_2	77	83	PF00069	0.283
MOD_Plk_4	112	118	PF00069	0.304
MOD_SUMO_rev_2	142	148	PF00179	0.288
TRG_DiLeu_BaLyEn_6	15	20	PF01217	0.519
TRG_DiLeu_BaLyEn_6	204	209	PF01217	0.447
TRG_ENDOCYTIC_2	60	63	PF00928	0.289
TRG_ER_diArg_1	205	207	PF00400	0.455
TRG_ER_diLys_1	211	215	PF00400	0.667
TRG_Pf-PMV_PEXEL_1	206	210	PF00026	0.247
TRG_Pf-PMV_PEXEL_1	57	62	PF00026	0.447

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZG2	Leptomonas seymouri	25%	99%
A0A0N1I2E2	Leptomonas seymouri	59%	100%
A0A0N1II77	Leptomonas seymouri	44%	100%
A0A0N1PA81	Leptomonas seymouri	42%	100%
A0A0S4JG68	Bodo saltans	34%	97%
A0A0S4JHA6	Bodo saltans	34%	100%
A0A1X0NKZ3	Trypanosomatidae	34%	97%
A0A3R7KJS3	Trypanosoma rangeli	32%	99%
A0A3S7X4D1	Leishmania donovani	41%	66%
A0A3S7X4G7	Leishmania donovani	92%	100%
A4HJ91	Leishmania braziliensis	68%	100%
A4HJ92	Leishmania braziliensis	43%	100%
A4I6N5	Leishmania infantum	92%	100%
A4I6N6	Leishmania infantum	41%	100%
C9ZM72	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
C9ZWV3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9B1Q8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
E9B1Q9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
Q4Q6F1	Leishmania major	40%	100%
Q90515	Takifugu rubripes	28%	100%
Q9P7I9	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
V5BK13	Trypanosoma cruzi	35%	97%