GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania major

UniProt:

Q4Q6F1_LEIMA

TriTrypDb:

LmjF.31.1120 , LMJLV39_310017600 , LMJSD75_310017200

Length:

156

Annotations

LeishMANIAdb annotations

Related to other eukaryotic COPII adaptors. Likely involved in ER to Golgi transport.. A Leishmaniid-specific subgroup of these proteins is missing the SigP (unclear topology and function). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 12, no: 8
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	3
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	3
GO:0005794	Golgi apparatus	5	3
GO:0016020	membrane	2	21
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	3
GO:0030135	coated vesicle	7	3
GO:0031410	cytoplasmic vesicle	6	3
GO:0031982	vesicle	4	3
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0097708	intracellular vesicle	5	3
GO:0110165	cellular anatomical entity	1	21

Expansion

Sequence features

Q4Q6F1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6F1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	3
GO:0006886	intracellular protein transport	4	3
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	3
GO:0006996	organelle organization	4	3
GO:0007030	Golgi organization	5	3
GO:0008104	protein localization	4	3
GO:0009987	cellular process	1	3
GO:0015031	protein transport	4	3
GO:0016043	cellular component organization	3	3
GO:0016192	vesicle-mediated transport	4	3
GO:0033036	macromolecule localization	2	3
GO:0045184	establishment of protein localization	3	3
GO:0046907	intracellular transport	3	3
GO:0048193	Golgi vesicle transport	5	3
GO:0051179	localization	1	3
GO:0051234	establishment of localization	2	3
GO:0051641	cellular localization	2	3
GO:0051649	establishment of localization in cell	3	3
GO:0070727	cellular macromolecule localization	3	3
GO:0071702	organic substance transport	4	3
GO:0071705	nitrogen compound transport	4	3
GO:0071840	cellular component organization or biogenesis	2	3

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	147	149	PF00675	0.271
CLV_NRD_NRD_1	153	155	PF00675	0.385
CLV_NRD_NRD_1	99	101	PF00675	0.396
CLV_PCSK_KEX2_1	147	149	PF00082	0.266
CLV_PCSK_KEX2_1	152	154	PF00082	0.393
CLV_PCSK_PC1ET2_1	152	154	PF00082	0.385
CLV_PCSK_PC7_1	148	154	PF00082	0.361
DEG_Nend_Nbox_1	1	3	PF02207	0.268
DOC_CKS1_1	5	10	PF01111	0.238
DOC_CYCLIN_RxL_1	42	52	PF00134	0.320
DOC_MAPK_FxFP_2	10	13	PF00069	0.279
DOC_PP1_RVXF_1	121	127	PF00149	0.253
DOC_PP1_RVXF_1	43	50	PF00149	0.297
DOC_PP2B_LxvP_1	36	39	PF13499	0.308
DOC_PP4_FxxP_1	10	13	PF00568	0.279
DOC_WW_Pin1_4	28	33	PF00397	0.364
DOC_WW_Pin1_4	4	9	PF00397	0.229
LIG_14-3-3_CterR_2	153	156	PF00244	0.547
LIG_BRCT_BRCA1_1	61	65	PF00533	0.317
LIG_FHA_1	19	25	PF00498	0.353
LIG_FHA_1	42	48	PF00498	0.305
LIG_FHA_2	113	119	PF00498	0.200
LIG_FHA_2	89	95	PF00498	0.308
LIG_LIR_Apic_2	7	13	PF02991	0.403
LIG_LIR_Gen_1	91	99	PF02991	0.322
LIG_Rb_LxCxE_1	11	31	PF01857	0.241
LIG_SH2_CRK	5	9	PF00017	0.238
LIG_SH2_GRB2like	35	38	PF00017	0.197
LIG_SH2_STAT5	35	38	PF00017	0.343
LIG_SUMO_SIM_par_1	56	62	PF11976	0.186
LIG_UBA3_1	92	101	PF00899	0.186
LIG_WRC_WIRS_1	132	137	PF05994	0.283
MOD_CK1_1	56	62	PF00069	0.275
MOD_CK2_1	112	118	PF00069	0.248
MOD_CK2_1	88	94	PF00069	0.284
MOD_GSK3_1	37	44	PF00069	0.360
MOD_GSK3_1	88	95	PF00069	0.373
MOD_NEK2_1	51	56	PF00069	0.289
MOD_NEK2_1	92	97	PF00069	0.337
MOD_PIKK_1	51	57	PF00454	0.333
MOD_PKA_2	18	24	PF00069	0.381
MOD_Plk_2-3	112	118	PF00069	0.174
MOD_Plk_4	125	131	PF00069	0.355
MOD_Plk_4	53	59	PF00069	0.294
MOD_Plk_4	88	94	PF00069	0.316
MOD_ProDKin_1	28	34	PF00069	0.364
MOD_ProDKin_1	4	10	PF00069	0.230
MOD_SUMO_rev_2	80	89	PF00179	0.289
TRG_DiLeu_BaEn_1	112	117	PF01217	0.157
TRG_ENDOCYTIC_2	35	38	PF00928	0.244
TRG_ER_diArg_1	14	17	PF00400	0.248
TRG_ER_diArg_1	146	148	PF00400	0.509
TRG_Pf-PMV_PEXEL_1	100	104	PF00026	0.397
TRG_Pf-PMV_PEXEL_1	114	118	PF00026	0.353
TRG_Pf-PMV_PEXEL_1	45	50	PF00026	0.535
TRG_Pf-PMV_PEXEL_1	68	72	PF00026	0.528

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZG2	Leptomonas seymouri	30%	72%
A0A0N1I2E2	Leptomonas seymouri	40%	73%
A0A0N1II77	Leptomonas seymouri	42%	76%
A0A0N1PA81	Leptomonas seymouri	41%	77%
A0A0S4JG68	Bodo saltans	28%	71%
A0A0S4JHA6	Bodo saltans	28%	77%
A0A0S4JYK0	Bodo saltans	34%	77%
A0A1X0NKZ3	Trypanosomatidae	31%	71%
A0A1X0NVX2	Trypanosomatidae	26%	69%
A0A3Q8ITC4	Leishmania donovani	31%	98%
A0A3R7KJS3	Trypanosoma rangeli	36%	72%
A0A3S7X4G7	Leishmania donovani	41%	73%
A0A422NB76	Trypanosoma rangeli	27%	71%
A4HJ91	Leishmania braziliensis	43%	73%
A4HJ92	Leishmania braziliensis	58%	100%
A4I6N5	Leishmania infantum	41%	73%
A4I6N6	Leishmania infantum	88%	75%
A4I824	Leishmania infantum	31%	98%
A7SXK3	Nematostella vectensis	25%	77%
C9ZWV3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	76%
D0AA90	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	68%
E9B1Q8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	73%
E9B1Q9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	75%
E9B2Y2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	98%
Q4Q6F2	Leishmania major	40%	73%
Q54BN0	Dictyostelium discoideum	29%	75%
Q769F9	Dictyostelium discoideum	31%	76%
V5BG96	Trypanosoma cruzi	32%	71%
V5BK13	Trypanosoma cruzi	31%	71%