H(+)-exporting diphosphatase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Pyrophosphate-energized vacuolar membrane proton pump 1, putative

Species:

Leishmania major

UniProt:

Q4Q6E1_LEIMA

TriTrypDb:

LmjF.31.1220 , LMJLV39_310018700 , LMJSD75_310018500

Length:

802

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 12, no: 0
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0000139	Golgi membrane	5	2
GO:0000331	contractile vacuole	6	2
GO:0005773	vacuole	5	2
GO:0016020	membrane	2	13
GO:0020022	acidocalcisome	5	2
GO:0031090	organelle membrane	3	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0110165	cellular anatomical entity	1	13

Expansion

Sequence features

Q4Q6E1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6E1

Function

Biological processes
Term	Name	Level	Count
GO:0006873	intracellular monoatomic ion homeostasis	4	2
GO:0006874	intracellular calcium ion homeostasis	7	2
GO:0006875	obsolete intracellular metal ion homeostasis	6	2
GO:0006885	regulation of pH	8	2
GO:0009987	cellular process	1	2
GO:0019725	cellular homeostasis	2	2
GO:0030003	intracellular monoatomic cation homeostasis	5	2
GO:0030004	obsolete cellular monovalent inorganic cation homeostasis	6	2
GO:0030641	regulation of cellular pH	7	2
GO:0042592	homeostatic process	3	2
GO:0048878	chemical homeostasis	4	2
GO:0050801	monoatomic ion homeostasis	5	2
GO:0051452	intracellular pH reduction	9	2
GO:0051453	regulation of intracellular pH	8	2
GO:0055065	obsolete metal ion homeostasis	7	2
GO:0055067	obsolete monovalent inorganic cation homeostasis	7	2
GO:0055074	calcium ion homeostasis	8	2
GO:0055080	monoatomic cation homeostasis	6	2
GO:0055082	intracellular chemical homeostasis	3	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	2
GO:0072507	obsolete divalent inorganic cation homeostasis	7	2
GO:0098771	inorganic ion homeostasis	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0004427	inorganic diphosphate phosphatase activity	6	13
GO:0005215	transporter activity	1	13
GO:0008324	monoatomic cation transmembrane transporter activity	4	13
GO:0009678	pyrophosphate hydrolysis-driven proton transmembrane transporter activity	5	13
GO:0015075	monoatomic ion transmembrane transporter activity	3	13
GO:0015078	proton transmembrane transporter activity	5	13
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	13
GO:0015399	primary active transmembrane transporter activity	4	13
GO:0016462	pyrophosphatase activity	5	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	13
GO:0022804	active transmembrane transporter activity	3	13
GO:0022853	active monoatomic ion transmembrane transporter activity	4	13
GO:0022857	transmembrane transporter activity	2	13
GO:0022890	inorganic cation transmembrane transporter activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	561	565	PF00656	0.507
CLV_NRD_NRD_1	646	648	PF00675	0.307
CLV_NRD_NRD_1	657	659	PF00675	0.307
CLV_PCSK_KEX2_1	403	405	PF00082	0.412
CLV_PCSK_KEX2_1	646	648	PF00082	0.307
CLV_PCSK_PC1ET2_1	403	405	PF00082	0.411
CLV_PCSK_SKI1_1	404	408	PF00082	0.406
CLV_PCSK_SKI1_1	560	564	PF00082	0.298
CLV_PCSK_SKI1_1	672	676	PF00082	0.357
CLV_Separin_Metazoa	106	110	PF03568	0.559
DEG_MDM2_SWIB_1	77	85	PF02201	0.401
DOC_CKS1_1	28	33	PF01111	0.549
DOC_CKS1_1	686	691	PF01111	0.284
DOC_CYCLIN_RxL_1	401	410	PF00134	0.540
DOC_MAPK_DCC_7	599	607	PF00069	0.340
DOC_MAPK_HePTP_8	773	785	PF00069	0.359
DOC_MAPK_MEF2A_6	4	12	PF00069	0.622
DOC_MAPK_MEF2A_6	599	607	PF00069	0.340
DOC_MAPK_MEF2A_6	764	773	PF00069	0.507
DOC_MAPK_MEF2A_6	776	785	PF00069	0.359
DOC_MAPK_MEF2A_6	792	800	PF00069	0.367
DOC_PP1_RVXF_1	217	223	PF00149	0.613
DOC_PP4_FxxP_1	649	652	PF00568	0.507
DOC_PP4_MxPP_1	678	681	PF00568	0.453
DOC_USP7_MATH_1	180	184	PF00917	0.387
DOC_USP7_MATH_1	352	356	PF00917	0.307
DOC_USP7_MATH_1	387	391	PF00917	0.516
DOC_USP7_MATH_1	550	554	PF00917	0.598
DOC_USP7_MATH_1	56	60	PF00917	0.447
DOC_USP7_MATH_1	581	585	PF00917	0.359
DOC_USP7_MATH_1	595	599	PF00917	0.307
DOC_USP7_MATH_1	708	712	PF00917	0.359
DOC_USP7_UBL2_3	741	745	PF12436	0.502
DOC_WW_Pin1_4	151	156	PF00397	0.314
DOC_WW_Pin1_4	161	166	PF00397	0.279
DOC_WW_Pin1_4	27	32	PF00397	0.570
DOC_WW_Pin1_4	685	690	PF00397	0.387
DOC_WW_Pin1_4	744	749	PF00397	0.507
DOC_WW_Pin1_4	88	93	PF00397	0.582
LIG_14-3-3_CanoR_1	117	125	PF00244	0.502
LIG_14-3-3_CanoR_1	227	233	PF00244	0.507
LIG_14-3-3_CanoR_1	55	65	PF00244	0.456
LIG_Actin_WH2_2	83	101	PF00022	0.639
LIG_BIR_II_1	1	5	PF00653	0.630
LIG_BIR_III_4	537	541	PF00653	0.359
LIG_BIR_III_4	567	571	PF00653	0.507
LIG_BRCT_BRCA1_1	334	338	PF00533	0.359
LIG_BRCT_BRCA1_1	781	785	PF00533	0.359
LIG_eIF4E_1	699	705	PF01652	0.280
LIG_FHA_1	102	108	PF00498	0.576
LIG_FHA_1	121	127	PF00498	0.540
LIG_FHA_1	245	251	PF00498	0.335
LIG_FHA_1	412	418	PF00498	0.387
LIG_FHA_1	426	432	PF00498	0.374
LIG_FHA_1	481	487	PF00498	0.503
LIG_FHA_1	504	510	PF00498	0.406
LIG_FHA_1	527	533	PF00498	0.359
LIG_FHA_1	561	567	PF00498	0.498
LIG_FHA_1	571	577	PF00498	0.498
LIG_FHA_1	60	66	PF00498	0.299
LIG_FHA_1	686	692	PF00498	0.265
LIG_FHA_2	259	265	PF00498	0.402
LIG_FHA_2	756	762	PF00498	0.507
LIG_LIR_Gen_1	129	140	PF02991	0.486
LIG_LIR_Gen_1	172	181	PF02991	0.387
LIG_LIR_Gen_1	264	274	PF02991	0.316
LIG_LIR_Gen_1	359	370	PF02991	0.307
LIG_LIR_Gen_1	86	92	PF02991	0.613
LIG_LIR_Nem_3	129	135	PF02991	0.359
LIG_LIR_Nem_3	138	143	PF02991	0.359
LIG_LIR_Nem_3	172	177	PF02991	0.382
LIG_LIR_Nem_3	264	269	PF02991	0.316
LIG_LIR_Nem_3	335	341	PF02991	0.359
LIG_LIR_Nem_3	364	370	PF02991	0.344
LIG_LIR_Nem_3	436	442	PF02991	0.328
LIG_LIR_Nem_3	464	470	PF02991	0.513
LIG_LIR_Nem_3	505	511	PF02991	0.404
LIG_LIR_Nem_3	537	542	PF02991	0.359
LIG_LIR_Nem_3	76	80	PF02991	0.362
LIG_LIR_Nem_3	782	788	PF02991	0.359
LIG_LIR_Nem_3	86	90	PF02991	0.567
LIG_LYPXL_S_1	366	370	PF13949	0.507
LIG_LYPXL_yS_3	367	370	PF13949	0.307
LIG_NRBOX	5	11	PF00104	0.619
LIG_Pex14_2	136	140	PF04695	0.359
LIG_Pex14_2	77	81	PF04695	0.380
LIG_Pex14_2	785	789	PF04695	0.406
LIG_SH2_CRK	115	119	PF00017	0.577
LIG_SH2_CRK	132	136	PF00017	0.231
LIG_SH2_CRK	467	471	PF00017	0.507
LIG_SH2_CRK	539	543	PF00017	0.507
LIG_SH2_GRB2like	196	199	PF00017	0.507
LIG_SH2_NCK_1	115	119	PF00017	0.598
LIG_SH2_NCK_1	266	270	PF00017	0.327
LIG_SH2_NCK_1	290	294	PF00017	0.516
LIG_SH2_NCK_1	699	703	PF00017	0.307
LIG_SH2_STAP1	266	270	PF00017	0.327
LIG_SH2_STAP1	290	294	PF00017	0.516
LIG_SH2_STAP1	457	461	PF00017	0.359
LIG_SH2_STAP1	462	466	PF00017	0.507
LIG_SH2_STAP1	663	667	PF00017	0.516
LIG_SH2_STAT3	732	735	PF00017	0.574
LIG_SH2_STAT5	102	105	PF00017	0.515
LIG_SH2_STAT5	112	115	PF00017	0.507
LIG_SH2_STAT5	196	199	PF00017	0.501
LIG_SH2_STAT5	254	257	PF00017	0.303
LIG_SH2_STAT5	290	293	PF00017	0.516
LIG_SH2_STAT5	457	460	PF00017	0.364
LIG_SH2_STAT5	462	465	PF00017	0.502
LIG_SH2_STAT5	493	496	PF00017	0.359
LIG_SH2_STAT5	508	511	PF00017	0.359
LIG_SH2_STAT5	596	599	PF00017	0.327
LIG_SH2_STAT5	699	702	PF00017	0.299
LIG_SH2_STAT5	732	735	PF00017	0.507
LIG_SH2_STAT5	83	86	PF00017	0.470
LIG_SH3_3	159	165	PF00018	0.366
LIG_SH3_3	21	27	PF00018	0.561
LIG_SH3_3	310	316	PF00018	0.598
LIG_SH3_3	420	426	PF00018	0.398
LIG_SH3_3	493	499	PF00018	0.348
LIG_SH3_3	597	603	PF00018	0.340
LIG_SH3_3	756	762	PF00018	0.507
LIG_SH3_3	781	787	PF00018	0.359
LIG_Sin3_3	11	18	PF02671	0.609
LIG_SUMO_SIM_anti_2	414	419	PF11976	0.405
LIG_SUMO_SIM_anti_2	64	70	PF11976	0.425
LIG_SUMO_SIM_par_1	367	372	PF11976	0.307
LIG_SUMO_SIM_par_1	64	70	PF11976	0.313
LIG_TRAF2_1	154	157	PF00917	0.390
LIG_TRAF2_1	469	472	PF00917	0.490
LIG_TYR_ITIM	113	118	PF00017	0.598
LIG_TYR_ITIM	491	496	PF00017	0.507
LIG_UBA3_1	241	249	PF00899	0.335
LIG_Vh1_VBS_1	370	388	PF01044	0.391
LIG_WRC_WIRS_1	338	343	PF05994	0.347
LIG_WRC_WIRS_1	84	89	PF05994	0.483
MOD_CDK_SPxK_1	88	94	PF00069	0.595
MOD_CK1_1	361	367	PF00069	0.369
MOD_CK1_1	528	534	PF00069	0.359
MOD_CK1_1	59	65	PF00069	0.417
MOD_CK1_1	627	633	PF00069	0.359
MOD_CK2_1	151	157	PF00069	0.390
MOD_CK2_1	254	260	PF00069	0.387
MOD_CK2_1	337	343	PF00069	0.359
MOD_Cter_Amidation	656	659	PF01082	0.398
MOD_GlcNHglycan	137	140	PF01048	0.512
MOD_GlcNHglycan	206	209	PF01048	0.359
MOD_GlcNHglycan	215	218	PF01048	0.371
MOD_GlcNHglycan	256	259	PF01048	0.557
MOD_GlcNHglycan	276	279	PF01048	0.382
MOD_GlcNHglycan	354	357	PF01048	0.518
MOD_GlcNHglycan	389	392	PF01048	0.316
MOD_GlcNHglycan	450	453	PF01048	0.347
MOD_GlcNHglycan	58	61	PF01048	0.605
MOD_GlcNHglycan	583	586	PF01048	0.359
MOD_GlcNHglycan	626	629	PF01048	0.367
MOD_GlcNHglycan	69	72	PF01048	0.264
MOD_GlcNHglycan	710	713	PF01048	0.359
MOD_GlcNHglycan	717	720	PF01048	0.359
MOD_GlcNHglycan	765	769	PF01048	0.307
MOD_GSK3_1	116	123	PF00069	0.516
MOD_GSK3_1	135	142	PF00069	0.453
MOD_GSK3_1	194	201	PF00069	0.527
MOD_GSK3_1	254	261	PF00069	0.406
MOD_GSK3_1	352	359	PF00069	0.301
MOD_GSK3_1	381	388	PF00069	0.486
MOD_GSK3_1	407	414	PF00069	0.358
MOD_GSK3_1	456	463	PF00069	0.348
MOD_GSK3_1	476	483	PF00069	0.377
MOD_GSK3_1	503	510	PF00069	0.361
MOD_GSK3_1	528	535	PF00069	0.372
MOD_GSK3_1	715	722	PF00069	0.368
MOD_N-GLC_1	167	172	PF02516	0.613
MOD_N-GLC_1	570	575	PF02516	0.307
MOD_NEK2_1	167	172	PF00069	0.411
MOD_NEK2_1	204	209	PF00069	0.598
MOD_NEK2_1	234	239	PF00069	0.372
MOD_NEK2_1	244	249	PF00069	0.351
MOD_NEK2_1	274	279	PF00069	0.359
MOD_NEK2_1	337	342	PF00069	0.359
MOD_NEK2_1	358	363	PF00069	0.333
MOD_NEK2_1	369	374	PF00069	0.295
MOD_NEK2_1	385	390	PF00069	0.177
MOD_NEK2_1	406	411	PF00069	0.359
MOD_NEK2_1	434	439	PF00069	0.398
MOD_NEK2_1	503	508	PF00069	0.348
MOD_NEK2_1	715	720	PF00069	0.453
MOD_NEK2_1	763	768	PF00069	0.507
MOD_NEK2_2	169	174	PF00069	0.327
MOD_PKA_2	116	122	PF00069	0.501
MOD_Plk_1	167	173	PF00069	0.413
MOD_Plk_1	434	440	PF00069	0.375
MOD_Plk_1	550	556	PF00069	0.574
MOD_Plk_4	139	145	PF00069	0.387
MOD_Plk_4	169	175	PF00069	0.320
MOD_Plk_4	361	367	PF00069	0.368
MOD_Plk_4	411	417	PF00069	0.348
MOD_Plk_4	492	498	PF00069	0.400
MOD_Plk_4	503	509	PF00069	0.354
MOD_Plk_4	61	67	PF00069	0.347
MOD_Plk_4	83	89	PF00069	0.597
MOD_ProDKin_1	151	157	PF00069	0.314
MOD_ProDKin_1	161	167	PF00069	0.279
MOD_ProDKin_1	27	33	PF00069	0.571
MOD_ProDKin_1	685	691	PF00069	0.387
MOD_ProDKin_1	744	750	PF00069	0.507
MOD_ProDKin_1	88	94	PF00069	0.595
MOD_SUMO_rev_2	128	133	PF00179	0.598
TRG_DiLeu_BaLyEn_6	365	370	PF01217	0.307
TRG_ENDOCYTIC_2	115	118	PF00928	0.565
TRG_ENDOCYTIC_2	130	133	PF00928	0.258
TRG_ENDOCYTIC_2	266	269	PF00928	0.327
TRG_ENDOCYTIC_2	367	370	PF00928	0.307
TRG_ENDOCYTIC_2	467	470	PF00928	0.507
TRG_ENDOCYTIC_2	493	496	PF00928	0.359
TRG_ENDOCYTIC_2	539	542	PF00928	0.507
TRG_ER_diArg_1	645	647	PF00400	0.507
TRG_Pf-PMV_PEXEL_1	560	564	PF00026	0.308

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5A1	Leptomonas seymouri	87%	100%
A0A0S4J2L1	Bodo saltans	76%	100%
A0A1X0NPP6	Trypanosomatidae	80%	95%
A0A3Q8IEU2	Leishmania donovani	97%	100%
A0A422NED3	Trypanosoma rangeli	77%	99%
A4HJA5	Leishmania braziliensis	88%	100%
A4I6P8	Leishmania infantum	97%	100%
C9ZM75	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	77%	97%
C9ZWU7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	77%	97%
E9B1S0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
O68460	Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)	41%	100%
P21616	Vigna radiata var. radiata	54%	100%
P31414	Arabidopsis thaliana	53%	100%
P60363	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)	38%	100%
Q06572	Hordeum vulgare	55%	100%
Q2RIS7	Moorella thermoacetica (strain ATCC 39073 / JCM 9320)	48%	100%
Q2RLE0	Moorella thermoacetica (strain ATCC 39073 / JCM 9320)	38%	100%
Q3AFC6	Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)	45%	100%
Q56ZN6	Arabidopsis thaliana	38%	100%
Q72Q29	Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)	51%	100%
Q82EJ8	Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)	36%	100%
Q82TF3	Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)	40%	100%
Q898Q9	Clostridium tetani (strain Massachusetts / E88)	47%	100%
Q89K83	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	40%	100%
Q8A294	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	39%	100%
Q8F641	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)	51%	100%
Q8G1E6	Brucella suis biovar 1 (strain 1330)	38%	100%
Q8KDT8	Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)	31%	100%
Q8KY01	Rhodopseudomonas palustris	40%	100%
Q8P5M6	Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)	41%	100%
Q8PH20	Xanthomonas axonopodis pv. citri (strain 306)	42%	100%
Q8PYZ7	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	43%	100%
Q8PYZ8	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	43%	100%
Q8RCX1	Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4)	40%	100%
Q8RHJ2	Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355)	44%	100%
Q8TJA8	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	44%	100%
Q8TJA9	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	45%	100%
Q8UG67	Agrobacterium fabrum (strain C58 / ATCC 33970)	37%	100%
Q8VNW3	Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)	37%	100%
Q8VRZ2	Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37)	38%	100%
Q8VRZ3	Rhizobium meliloti (strain 1021)	40%	100%
Q8YGH4	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)	38%	100%
Q8ZWI8	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	39%	100%
Q983A3	Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)	40%	100%
Q9A8J0	Caulobacter vibrioides (strain ATCC 19089 / CB15)	38%	100%
Q9FWR2	Arabidopsis thaliana	38%	100%
Q9S5X0	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	39%	100%
Q9X913	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	36%	100%
V5BFF6	Trypanosoma cruzi	78%	99%