Diphthine methyl ester synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diphthine synthase-like protein

Species:

Leishmania major

UniProt:

Q4Q6A1_LEIMA

TriTrypDb:

LmjF.31.1610 , LMJLV39_310024000 , LMJSD75_310023300

Length:

271

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q6A1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q6A1

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	12
GO:0006448	regulation of translational elongation	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009889	regulation of biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0010468	regulation of gene expression	5	12
GO:0010556	regulation of macromolecule biosynthetic process	5	12
GO:0010608	post-transcriptional regulation of gene expression	6	12
GO:0017182	peptidyl-diphthamide metabolic process	7	12
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	12
GO:0018193	peptidyl-amino acid modification	5	12
GO:0018202	peptidyl-histidine modification	6	12
GO:0019222	regulation of metabolic process	3	12
GO:0019538	protein metabolic process	3	12
GO:0031323	regulation of cellular metabolic process	4	12
GO:0031326	regulation of cellular biosynthetic process	5	12
GO:0032259	methylation	2	12
GO:0034248	regulation of amide metabolic process	5	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0051171	regulation of nitrogen compound metabolic process	4	12
GO:0051246	regulation of protein metabolic process	5	12
GO:0060255	regulation of macromolecule metabolic process	4	12
GO:0065007	biological regulation	1	12
GO:0071704	organic substance metabolic process	2	12
GO:0080090	regulation of primary metabolic process	4	12
GO:1900247	regulation of cytoplasmic translational elongation	8	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004164	diphthine synthase activity	6	12
GO:0008168	methyltransferase activity	4	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	177	179	PF00675	0.237
CLV_PCSK_SKI1_1	185	189	PF00082	0.227
CLV_PCSK_SKI1_1	235	239	PF00082	0.315
CLV_PCSK_SKI1_1	75	79	PF00082	0.223
CLV_Separin_Metazoa	182	186	PF03568	0.423
DEG_Nend_Nbox_1	1	3	PF02207	0.413
DOC_MAPK_gen_1	72	81	PF00069	0.452
DOC_MAPK_MEF2A_6	125	134	PF00069	0.432
DOC_MAPK_MEF2A_6	235	242	PF00069	0.562
DOC_MAPK_MEF2A_6	72	81	PF00069	0.445
DOC_PP4_FxxP_1	242	245	PF00568	0.311
DOC_USP7_MATH_1	210	214	PF00917	0.477
DOC_USP7_MATH_1	245	249	PF00917	0.288
DOC_USP7_MATH_1	33	37	PF00917	0.438
LIG_BRCT_BRCA1_1	130	134	PF00533	0.413
LIG_BRCT_BRCA1_1	238	242	PF00533	0.404
LIG_Clathr_ClatBox_1	159	163	PF01394	0.413
LIG_Clathr_ClatBox_1	237	241	PF01394	0.409
LIG_deltaCOP1_diTrp_1	137	144	PF00928	0.423
LIG_FHA_1	156	162	PF00498	0.413
LIG_FHA_1	212	218	PF00498	0.413
LIG_FHA_1	33	39	PF00498	0.413
LIG_FHA_2	231	237	PF00498	0.480
LIG_FHA_2	87	93	PF00498	0.413
LIG_LIR_Apic_2	239	245	PF02991	0.326
LIG_LIR_Gen_1	142	153	PF02991	0.437
LIG_LIR_Gen_1	32	39	PF02991	0.435
LIG_LIR_Nem_3	142	148	PF02991	0.413
LIG_LIR_Nem_3	32	37	PF02991	0.435
LIG_SH2_CRK	186	190	PF00017	0.435
LIG_SH2_CRK	50	54	PF00017	0.423
LIG_SH2_SRC	181	184	PF00017	0.413
LIG_SH2_STAP1	34	38	PF00017	0.436
LIG_SH2_STAT5	34	37	PF00017	0.423
LIG_SUMO_SIM_anti_2	158	163	PF11976	0.413
LIG_SUMO_SIM_par_1	157	163	PF11976	0.413
LIG_SUMO_SIM_par_1	194	200	PF11976	0.423
LIG_SUMO_SIM_par_1	236	241	PF11976	0.564
LIG_SUMO_SIM_par_1	52	58	PF11976	0.414
LIG_SUMO_SIM_par_1	77	83	PF11976	0.423
LIG_UBA3_1	161	167	PF00899	0.415
LIG_WRC_WIRS_1	34	39	PF05994	0.413
LIG_WW_1	183	186	PF00397	0.423
LIG_WW_3	182	186	PF00397	0.423
MOD_CK1_1	13	19	PF00069	0.437
MOD_CK2_1	140	146	PF00069	0.474
MOD_CK2_1	38	44	PF00069	0.408
MOD_Cter_Amidation	176	179	PF01082	0.229
MOD_GlcNHglycan	63	66	PF01048	0.213
MOD_N-GLC_1	108	113	PF02516	0.213
MOD_NEK2_1	38	43	PF00069	0.408
MOD_NEK2_1	61	66	PF00069	0.496
MOD_NEK2_1	85	90	PF00069	0.406
MOD_PIKK_1	223	229	PF00454	0.414
MOD_Plk_1	108	114	PF00069	0.413
MOD_Plk_1	13	19	PF00069	0.413
MOD_Plk_2-3	236	242	PF00069	0.509
MOD_Plk_4	108	114	PF00069	0.413
MOD_Plk_4	140	146	PF00069	0.425
MOD_Plk_4	155	161	PF00069	0.437
MOD_Plk_4	245	251	PF00069	0.311
MOD_Plk_4	33	39	PF00069	0.430
MOD_SUMO_rev_2	64	73	PF00179	0.471
TRG_ENDOCYTIC_2	145	148	PF00928	0.413
TRG_ENDOCYTIC_2	186	189	PF00928	0.413
TRG_ENDOCYTIC_2	34	37	PF00928	0.436
TRG_Pf-PMV_PEXEL_1	178	182	PF00026	0.280

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I7M8	Leptomonas seymouri	84%	100%
A0A0S4J5A5	Bodo saltans	71%	100%
A0A1X0NPT2	Trypanosomatidae	77%	100%
A0A3S7X4K3	Leishmania donovani	99%	100%
A0A422NZ48	Trypanosoma rangeli	75%	100%
A0B879	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	35%	100%
A1RU15	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	28%	100%
A2SQF6	Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)	40%	100%
A3CWF9	Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)	38%	100%
A3DLV7	Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / JCM 9404 / F1)	29%	100%
A4FYP1	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	35%	100%
A4HJE1	Leishmania braziliensis	92%	100%
A4I6T9	Leishmania infantum	99%	100%
A4YHI8	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	28%	100%
A6US81	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	33%	100%
A6UU49	Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)	35%	100%
A6VJP1	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	34%	100%
A7IA21	Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)	41%	100%
A9A6D8	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	33%	100%
B0R4W9	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	36%	100%
B1YAU2	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	26%	100%
B6YXP9	Thermococcus onnurineus (strain NA1)	38%	100%
B8GIF8	Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)	42%	100%
B9LV13	Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)	34%	100%
C3MPQ5	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	25%	100%
C3MYP9	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	25%	100%
C3N5D1	Sulfolobus islandicus (strain M.16.27)	25%	100%
C3NDY5	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	25%	100%
C3NHR8	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	25%	100%
C4KGZ7	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	25%	100%
C5A3K4	Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)	39%	100%
C9ZMB0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	100%
E9B1W1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O27902	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	34%	100%
O29866	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	35%	100%
O58456	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	38%	100%
O74898	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	53%	96%
O81769	Arabidopsis thaliana	55%	98%
P0CU37	Candida albicans (strain SC5314 / ATCC MYA-2876)	52%	91%
P32469	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	55%	90%
Q0W085	Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)	42%	100%
Q12XB4	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	38%	100%
Q18JS3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	32%	100%
Q2FQ45	Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)	39%	100%
Q2NFJ8	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	33%	100%
Q3IS55	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	36%	100%
Q467Z4	Methanosarcina barkeri (strain Fusaro / DSM 804)	39%	100%
Q4HZI0	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	51%	94%
Q58670	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	37%	100%
Q59NX9	Candida albicans (strain SC5314 / ATCC MYA-2876)	52%	91%
Q5BFG0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	55%	95%
Q5E982	Bos taurus	54%	95%
Q5JFE7	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	38%	100%
Q5V2B7	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	37%	100%
Q6BN80	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	53%	90%
Q6C1E0	Yarrowia lipolytica (strain CLIB 122 / E 150)	50%	90%
Q6CIZ1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	55%	91%
Q6FXK9	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	56%	91%
Q6LZN6	Methanococcus maripaludis (strain S2 / LL)	34%	100%
Q6MY91	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	55%	95%
Q754E7	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	57%	91%
Q75JG8	Dictyostelium discoideum	55%	99%
Q7S949	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	51%	94%
Q8PUH9	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	41%	100%
Q8TR14	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	41%	100%
Q8TXC7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	29%	100%
Q8U377	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	36%	100%
Q8ZXR9	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	31%	100%
Q971V1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	28%	100%
Q97TX8	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	25%	100%
Q9CWQ0	Mus musculus	55%	96%
Q9H2P9	Homo sapiens	53%	95%
Q9HJT0	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	27%	100%
Q9HQK6	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	36%	100%
Q9UZ31	Pyrococcus abyssi (strain GE5 / Orsay)	35%	100%
V5DGG4	Trypanosoma cruzi	76%	100%