Putative dihydroceramide synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Sphingosine N-acyltransferase, putative

Species:

Leishmania major

UniProt:

Q4Q684_LEIMA

TriTrypDb:

LmjF.31.1780 , LMJLV39_310025800 , LMJSD75_310025300

Length:

461

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0016020	membrane	2	14
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	14

Expansion

Sequence features

Q4Q684

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q684

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	14
GO:0006643	membrane lipid metabolic process	4	14
GO:0006665	sphingolipid metabolic process	4	14
GO:0006672	ceramide metabolic process	4	14
GO:0006807	nitrogen compound metabolic process	2	14
GO:0008152	metabolic process	1	14
GO:0008610	lipid biosynthetic process	4	14
GO:0009058	biosynthetic process	2	14
GO:0009987	cellular process	1	14
GO:0030148	sphingolipid biosynthetic process	5	14
GO:0034641	cellular nitrogen compound metabolic process	3	14
GO:0043603	amide metabolic process	3	14
GO:0043604	amide biosynthetic process	4	14
GO:0044237	cellular metabolic process	2	14
GO:0044238	primary metabolic process	2	14
GO:0044249	cellular biosynthetic process	3	14
GO:0044255	cellular lipid metabolic process	3	14
GO:0044271	cellular nitrogen compound biosynthetic process	4	14
GO:0046467	membrane lipid biosynthetic process	4	14
GO:0046513	ceramide biosynthetic process	5	14
GO:0071704	organic substance metabolic process	2	14
GO:1901564	organonitrogen compound metabolic process	3	14
GO:1901566	organonitrogen compound biosynthetic process	4	14
GO:1901576	organic substance biosynthetic process	3	14

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0016410	N-acyltransferase activity	5	14
GO:0016740	transferase activity	2	14
GO:0016746	acyltransferase activity	3	14
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	14
GO:0050291	sphingosine N-acyltransferase activity	6	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	246	252	PF00089	0.548
CLV_NRD_NRD_1	137	139	PF00675	0.327
CLV_NRD_NRD_1	281	283	PF00675	0.268
CLV_NRD_NRD_1	298	300	PF00675	0.258
CLV_NRD_NRD_1	35	37	PF00675	0.637
CLV_NRD_NRD_1	453	455	PF00675	0.590
CLV_NRD_NRD_1	458	460	PF00675	0.579
CLV_PCSK_KEX2_1	221	223	PF00082	0.237
CLV_PCSK_KEX2_1	281	283	PF00082	0.277
CLV_PCSK_KEX2_1	35	37	PF00082	0.635
CLV_PCSK_KEX2_1	453	455	PF00082	0.591
CLV_PCSK_KEX2_1	458	460	PF00082	0.581
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.254
CLV_PCSK_PC1ET2_1	458	460	PF00082	0.485
CLV_PCSK_PC7_1	454	460	PF00082	0.490
CLV_PCSK_SKI1_1	140	144	PF00082	0.361
CLV_PCSK_SKI1_1	218	222	PF00082	0.319
CLV_PCSK_SKI1_1	300	304	PF00082	0.305
DOC_ANK_TNKS_1	10	17	PF00023	0.353
DOC_CYCLIN_yCln2_LP_2	185	191	PF00134	0.355
DOC_MAPK_gen_1	136	144	PF00069	0.508
DOC_MAPK_MEF2A_6	143	152	PF00069	0.479
DOC_PP2B_LxvP_1	183	186	PF13499	0.348
DOC_PP2B_LxvP_1	25	28	PF13499	0.391
DOC_PP4_FxxP_1	171	174	PF00568	0.266
DOC_SPAK_OSR1_1	104	108	PF12202	0.561
DOC_USP7_MATH_1	18	22	PF00917	0.426
DOC_USP7_MATH_1	431	435	PF00917	0.723
DOC_USP7_UBL2_3	139	143	PF12436	0.597
DOC_USP7_UBL2_3	296	300	PF12436	0.568
DOC_WW_Pin1_4	131	136	PF00397	0.591
DOC_WW_Pin1_4	14	19	PF00397	0.406
DOC_WW_Pin1_4	340	345	PF00397	0.338
LIG_14-3-3_CanoR_1	299	303	PF00244	0.565
LIG_14-3-3_CanoR_1	305	313	PF00244	0.550
LIG_14-3-3_CanoR_1	35	39	PF00244	0.450
LIG_14-3-3_CanoR_1	4	12	PF00244	0.396
LIG_14-3-3_CanoR_1	42	46	PF00244	0.409
LIG_Actin_WH2_2	373	391	PF00022	0.426
LIG_BIR_II_1	1	5	PF00653	0.406
LIG_BRCT_BRCA1_1	156	160	PF00533	0.287
LIG_Clathr_ClatBox_1	213	217	PF01394	0.432
LIG_EH1_1	230	238	PF00400	0.334
LIG_FHA_1	232	238	PF00498	0.289
LIG_FHA_1	7	13	PF00498	0.467
LIG_GBD_Chelix_1	356	364	PF00786	0.348
LIG_LIR_Gen_1	100	109	PF02991	0.589
LIG_LIR_Gen_1	17	27	PF02991	0.348
LIG_LIR_Gen_1	44	55	PF02991	0.382
LIG_LIR_Nem_3	100	105	PF02991	0.618
LIG_LIR_Nem_3	157	163	PF02991	0.364
LIG_LIR_Nem_3	167	173	PF02991	0.245
LIG_LIR_Nem_3	17	22	PF02991	0.351
LIG_LIR_Nem_3	223	228	PF02991	0.467
LIG_LIR_Nem_3	247	253	PF02991	0.246
LIG_LIR_Nem_3	44	50	PF02991	0.353
LIG_LIR_Nem_3	52	57	PF02991	0.324
LIG_LYPXL_S_1	81	85	PF13949	0.313
LIG_LYPXL_yS_3	82	85	PF13949	0.313
LIG_NRBOX	355	361	PF00104	0.276
LIG_Pex14_1	149	153	PF04695	0.301
LIG_Pex14_1	354	358	PF04695	0.391
LIG_Pex14_2	320	324	PF04695	0.276
LIG_REV1ctd_RIR_1	322	332	PF16727	0.304
LIG_SH2_CRK	108	112	PF00017	0.564
LIG_SH2_CRK	189	193	PF00017	0.336
LIG_SH2_CRK	19	23	PF00017	0.386
LIG_SH2_CRK	330	334	PF00017	0.433
LIG_SH2_NCK_1	189	193	PF00017	0.348
LIG_SH2_STAP1	19	23	PF00017	0.415
LIG_SH2_STAT3	162	165	PF00017	0.291
LIG_SH2_STAT3	273	276	PF00017	0.548
LIG_SH2_STAT5	154	157	PF00017	0.301
LIG_SH2_STAT5	163	166	PF00017	0.330
LIG_SH2_STAT5	206	209	PF00017	0.279
LIG_SH2_STAT5	253	256	PF00017	0.281
LIG_SH2_STAT5	26	29	PF00017	0.450
LIG_SH2_STAT5	330	333	PF00017	0.421
LIG_SH2_STAT5	96	99	PF00017	0.553
LIG_SH3_3	185	191	PF00018	0.254
LIG_SH3_3	77	83	PF00018	0.313
LIG_SUMO_SIM_anti_2	234	242	PF11976	0.303
LIG_SUMO_SIM_anti_2	74	79	PF11976	0.296
LIG_SUMO_SIM_par_1	234	242	PF11976	0.303
LIG_SUMO_SIM_par_1	261	268	PF11976	0.321
LIG_SUMO_SIM_par_1	373	379	PF11976	0.416
LIG_TRAF2_1	290	293	PF00917	0.533
LIG_TRAF2_1	418	421	PF00917	0.747
LIG_TYR_ITIM	80	85	PF00017	0.421
LIG_UBA3_1	213	221	PF00899	0.367
MOD_CDK_SPK_2	131	136	PF00069	0.591
MOD_CDK_SPK_2	340	345	PF00069	0.263
MOD_CDK_SPxxK_3	131	138	PF00069	0.547
MOD_CDK_SPxxK_3	340	347	PF00069	0.338
MOD_CK1_1	34	40	PF00069	0.410
MOD_CK1_1	411	417	PF00069	0.629
MOD_CK1_1	439	445	PF00069	0.766
MOD_CK1_1	6	12	PF00069	0.446
MOD_CK2_1	18	24	PF00069	0.389
MOD_CK2_1	390	396	PF00069	0.673
MOD_CK2_1	406	412	PF00069	0.661
MOD_GlcNHglycan	124	127	PF01048	0.516
MOD_GlcNHglycan	412	416	PF01048	0.475
MOD_GlcNHglycan	51	54	PF01048	0.611
MOD_GlcNHglycan	68	71	PF01048	0.519
MOD_GSK3_1	110	117	PF00069	0.668
MOD_GSK3_1	14	21	PF00069	0.410
MOD_GSK3_1	263	270	PF00069	0.405
MOD_GSK3_1	336	343	PF00069	0.299
MOD_GSK3_1	37	44	PF00069	0.420
MOD_GSK3_1	396	403	PF00069	0.698
MOD_GSK3_1	406	413	PF00069	0.653
MOD_GSK3_1	436	443	PF00069	0.772
MOD_GSK3_1	444	451	PF00069	0.795
MOD_GSK3_1	45	52	PF00069	0.406
MOD_LATS_1	406	412	PF00433	0.629
MOD_N-GLC_1	31	36	PF02516	0.616
MOD_N-GLC_1	448	453	PF02516	0.526
MOD_N-GLC_1	45	50	PF02516	0.507
MOD_N-GLC_2	314	316	PF02516	0.391
MOD_NEK2_1	148	153	PF00069	0.405
MOD_NEK2_1	155	160	PF00069	0.363
MOD_NEK2_1	265	270	PF00069	0.320
MOD_NEK2_1	378	383	PF00069	0.582
MOD_NEK2_1	410	415	PF00069	0.682
MOD_NEK2_1	440	445	PF00069	0.773
MOD_NEK2_2	336	341	PF00069	0.304
MOD_PIKK_1	448	454	PF00454	0.689
MOD_PKA_1	453	459	PF00069	0.731
MOD_PKA_2	298	304	PF00069	0.561
MOD_PKA_2	3	9	PF00069	0.398
MOD_PKA_2	34	40	PF00069	0.485
MOD_PKA_2	41	47	PF00069	0.465
MOD_PKA_2	431	437	PF00069	0.656
MOD_PKA_2	453	459	PF00069	0.789
MOD_PKB_1	282	290	PF00069	0.444
MOD_Plk_1	177	183	PF00069	0.348
MOD_Plk_1	73	79	PF00069	0.255
MOD_Plk_2-3	390	396	PF00069	0.597
MOD_Plk_4	148	154	PF00069	0.378
MOD_Plk_4	209	215	PF00069	0.318
MOD_Plk_4	232	238	PF00069	0.365
MOD_Plk_4	298	304	PF00069	0.499
MOD_Plk_4	73	79	PF00069	0.271
MOD_ProDKin_1	131	137	PF00069	0.586
MOD_ProDKin_1	14	20	PF00069	0.413
MOD_ProDKin_1	340	346	PF00069	0.338
TRG_ENDOCYTIC_2	108	111	PF00928	0.503
TRG_ENDOCYTIC_2	19	22	PF00928	0.355
TRG_ENDOCYTIC_2	253	256	PF00928	0.276
TRG_ENDOCYTIC_2	370	373	PF00928	0.369
TRG_ENDOCYTIC_2	82	85	PF00928	0.424
TRG_ENDOCYTIC_2	86	89	PF00928	0.403
TRG_ER_diArg_1	135	138	PF00400	0.624
TRG_NLS_MonoExtN_4	135	142	PF00514	0.544
TRG_Pf-PMV_PEXEL_1	408	412	PF00026	0.426
TRG_Pf-PMV_PEXEL_1	95	100	PF00026	0.397

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2N8	Leptomonas seymouri	63%	100%
A0A0S4JEY3	Bodo saltans	32%	100%
A0A0S4JIF9	Bodo saltans	34%	100%
A0A1X0NPU5	Trypanosomatidae	41%	100%
A0A1X0NPY1	Trypanosomatidae	44%	100%
A0A3S7X4J5	Leishmania donovani	95%	100%
A0A422MNN3	Trypanosoma rangeli	44%	100%
A4HJG0	Leishmania braziliensis	69%	100%
A4I6V5	Leishmania infantum	96%	100%
C9ZMC0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
C9ZWR6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9B1X8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%