Protein kinase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tousled-like kinase II, putative

Species:

Leishmania major

UniProt:

Q4Q5X3_LEIMA

TriTrypDb:

LmjF.31.2860 , LMJLV39_310039300 , LMJSD75_310039300

Length:

676

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q5X3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5X3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006260	DNA replication	5	2
GO:0006281	DNA repair	5	2
GO:0006468	protein phosphorylation	5	12
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0007059	chromosome segregation	2	2
GO:0007165	signal transduction	2	2
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016310	phosphorylation	5	12
GO:0018105	peptidyl-serine phosphorylation	6	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018209	peptidyl-serine modification	6	2
GO:0019538	protein metabolic process	3	12
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0035556	intracellular signal transduction	3	2
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004672	protein kinase activity	3	12
GO:0004674	protein serine/threonine kinase activity	4	2
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140096	catalytic activity, acting on a protein	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	147	151	PF00656	0.469
CLV_C14_Caspase3-7	21	25	PF00656	0.579
CLV_C14_Caspase3-7	287	291	PF00656	0.532
CLV_C14_Caspase3-7	68	72	PF00656	0.574
CLV_C14_Caspase3-7	96	100	PF00656	0.705
CLV_NRD_NRD_1	123	125	PF00675	0.516
CLV_NRD_NRD_1	169	171	PF00675	0.522
CLV_NRD_NRD_1	185	187	PF00675	0.510
CLV_NRD_NRD_1	211	213	PF00675	0.371
CLV_NRD_NRD_1	335	337	PF00675	0.335
CLV_NRD_NRD_1	585	587	PF00675	0.262
CLV_NRD_NRD_1	644	646	PF00675	0.493
CLV_PCSK_KEX2_1	123	125	PF00082	0.656
CLV_PCSK_KEX2_1	169	171	PF00082	0.522
CLV_PCSK_KEX2_1	17	19	PF00082	0.579
CLV_PCSK_KEX2_1	211	213	PF00082	0.371
CLV_PCSK_KEX2_1	254	256	PF00082	0.342
CLV_PCSK_KEX2_1	335	337	PF00082	0.335
CLV_PCSK_KEX2_1	452	454	PF00082	0.269
CLV_PCSK_KEX2_1	575	577	PF00082	0.239
CLV_PCSK_KEX2_1	585	587	PF00082	0.239
CLV_PCSK_KEX2_1	644	646	PF00082	0.493
CLV_PCSK_PC1ET2_1	17	19	PF00082	0.579
CLV_PCSK_PC1ET2_1	254	256	PF00082	0.409
CLV_PCSK_PC1ET2_1	452	454	PF00082	0.269
CLV_PCSK_PC1ET2_1	575	577	PF00082	0.310
CLV_PCSK_PC7_1	207	213	PF00082	0.365
CLV_PCSK_SKI1_1	123	127	PF00082	0.506
CLV_PCSK_SKI1_1	211	215	PF00082	0.377
CLV_PCSK_SKI1_1	242	246	PF00082	0.444
CLV_PCSK_SKI1_1	327	331	PF00082	0.363
CLV_PCSK_SKI1_1	356	360	PF00082	0.273
CLV_PCSK_SKI1_1	455	459	PF00082	0.304
CLV_PCSK_SKI1_1	463	467	PF00082	0.239
DEG_APCC_DBOX_1	355	363	PF00400	0.273
DEG_Nend_UBRbox_2	1	3	PF02207	0.715
DEG_SCF_FBW7_1	603	609	PF00400	0.232
DEG_SPOP_SBC_1	85	89	PF00917	0.743
DOC_ANK_TNKS_1	575	582	PF00023	0.239
DOC_CKS1_1	45	50	PF01111	0.621
DOC_CKS1_1	603	608	PF01111	0.232
DOC_CYCLIN_RxL_1	239	250	PF00134	0.345
DOC_CYCLIN_yCln2_LP_2	479	485	PF00134	0.382
DOC_CYCLIN_yCln2_LP_2	665	668	PF00134	0.637
DOC_MAPK_gen_1	169	176	PF00069	0.520
DOC_MAPK_gen_1	240	248	PF00069	0.374
DOC_MAPK_MEF2A_6	240	248	PF00069	0.416
DOC_MAPK_MEF2A_6	430	438	PF00069	0.382
DOC_PP1_RVXF_1	461	467	PF00149	0.382
DOC_PP1_RVXF_1	596	603	PF00149	0.273
DOC_PP2B_LxvP_1	346	349	PF13499	0.430
DOC_PP2B_LxvP_1	665	668	PF13499	0.647
DOC_PP4_FxxP_1	125	128	PF00568	0.653
DOC_USP7_MATH_1	264	268	PF00917	0.508
DOC_USP7_MATH_1	606	610	PF00917	0.264
DOC_USP7_MATH_1	65	69	PF00917	0.666
DOC_USP7_MATH_1	93	97	PF00917	0.651
DOC_USP7_UBL2_3	328	332	PF12436	0.373
DOC_WW_Pin1_4	44	49	PF00397	0.676
DOC_WW_Pin1_4	478	483	PF00397	0.382
DOC_WW_Pin1_4	549	554	PF00397	0.239
DOC_WW_Pin1_4	602	607	PF00397	0.232
DOC_WW_Pin1_4	660	665	PF00397	0.708
DOC_WW_Pin1_4	670	675	PF00397	0.710
DOC_WW_Pin1_4	86	91	PF00397	0.699
LIG_14-3-3_CanoR_1	240	245	PF00244	0.404
LIG_14-3-3_CanoR_1	302	309	PF00244	0.476
LIG_14-3-3_CanoR_1	474	480	PF00244	0.382
LIG_Actin_WH2_2	306	321	PF00022	0.423
LIG_APCC_ABBA_1	493	498	PF00400	0.382
LIG_APCC_ABBAyCdc20_2	453	459	PF00400	0.321
LIG_BIR_III_2	671	675	PF00653	0.569
LIG_deltaCOP1_diTrp_1	370	375	PF00928	0.239
LIG_deltaCOP1_diTrp_1	560	568	PF00928	0.239
LIG_eIF4E_1	357	363	PF01652	0.239
LIG_EVH1_2	543	547	PF00568	0.239
LIG_FHA_1	137	143	PF00498	0.563
LIG_FHA_1	241	247	PF00498	0.447
LIG_FHA_1	308	314	PF00498	0.483
LIG_FHA_1	398	404	PF00498	0.315
LIG_FHA_1	44	50	PF00498	0.691
LIG_FHA_1	550	556	PF00498	0.239
LIG_FHA_1	603	609	PF00498	0.268
LIG_FHA_1	86	92	PF00498	0.676
LIG_FHA_2	310	316	PF00498	0.484
LIG_FHA_2	319	325	PF00498	0.522
LIG_FHA_2	348	354	PF00498	0.468
LIG_FHA_2	499	505	PF00498	0.382
LIG_Integrin_RGD_1	430	432	PF01839	0.382
LIG_LIR_Apic_2	538	544	PF02991	0.239
LIG_LIR_Gen_1	189	199	PF02991	0.472
LIG_LIR_Gen_1	243	251	PF02991	0.414
LIG_LIR_Gen_1	342	351	PF02991	0.349
LIG_LIR_Gen_1	355	363	PF02991	0.194
LIG_LIR_Gen_1	422	429	PF02991	0.239
LIG_LIR_Gen_1	432	441	PF02991	0.239
LIG_LIR_LC3C_4	52	56	PF02991	0.637
LIG_LIR_Nem_3	189	194	PF02991	0.442
LIG_LIR_Nem_3	243	248	PF02991	0.419
LIG_LIR_Nem_3	342	348	PF02991	0.366
LIG_LIR_Nem_3	369	375	PF02991	0.239
LIG_LIR_Nem_3	422	428	PF02991	0.239
LIG_LIR_Nem_3	432	438	PF02991	0.239
LIG_LIR_Nem_3	536	542	PF02991	0.276
LIG_NRBOX	358	364	PF00104	0.273
LIG_Pex14_2	368	372	PF04695	0.239
LIG_Rb_pABgroove_1	419	427	PF01858	0.239
LIG_SH2_CRK	133	137	PF00017	0.517
LIG_SH2_CRK	569	573	PF00017	0.239
LIG_SH2_GRB2like	441	444	PF00017	0.315
LIG_SH2_SRC	541	544	PF00017	0.239
LIG_SH2_STAT5	357	360	PF00017	0.271
LIG_SH2_STAT5	382	385	PF00017	0.278
LIG_SH2_STAT5	402	405	PF00017	0.109
LIG_SH2_STAT5	450	453	PF00017	0.402
LIG_SH2_STAT5	475	478	PF00017	0.239
LIG_SH2_STAT5	541	544	PF00017	0.249
LIG_SH2_STAT5	638	641	PF00017	0.341
LIG_SH3_2	13	18	PF14604	0.503
LIG_SH3_3	42	48	PF00018	0.616
LIG_SH3_3	55	61	PF00018	0.494
LIG_SH3_3	600	606	PF00018	0.239
LIG_SH3_3	661	667	PF00018	0.630
LIG_SH3_3	67	73	PF00018	0.637
LIG_SH3_3	7	13	PF00018	0.519
LIG_SUMO_SIM_anti_2	409	414	PF11976	0.273
LIG_SUMO_SIM_par_1	52	59	PF11976	0.725
LIG_TRAF2_1	19	22	PF00917	0.730
LIG_TRAF2_1	26	29	PF00917	0.754
LIG_TRAF2_1	273	276	PF00917	0.536
LIG_TRFH_1	486	490	PF08558	0.239
LIG_TYR_ITIM	567	572	PF00017	0.239
LIG_UBA3_1	359	365	PF00899	0.239
LIG_UBA3_1	502	506	PF00899	0.382
LIG_UBA3_1	567	575	PF00899	0.364
MOD_CDK_SPK_2	549	554	PF00069	0.239
MOD_CDK_SPK_2	602	607	PF00069	0.232
MOD_CK1_1	114	120	PF00069	0.645
MOD_CK1_1	31	37	PF00069	0.704
MOD_CK1_1	44	50	PF00069	0.665
MOD_CK1_1	478	484	PF00069	0.379
MOD_CK1_1	533	539	PF00069	0.273
MOD_CK1_1	596	602	PF00069	0.239
MOD_CK1_1	643	649	PF00069	0.522
MOD_CK1_1	660	666	PF00069	0.437
MOD_CK2_1	148	154	PF00069	0.547
MOD_CK2_1	318	324	PF00069	0.541
MOD_CK2_1	347	353	PF00069	0.473
MOD_CK2_1	434	440	PF00069	0.239
MOD_Cter_Amidation	167	170	PF01082	0.451
MOD_Cter_Amidation	573	576	PF01082	0.239
MOD_GlcNHglycan	116	119	PF01048	0.661
MOD_GlcNHglycan	200	203	PF01048	0.498
MOD_GlcNHglycan	280	284	PF01048	0.589
MOD_GlcNHglycan	562	565	PF01048	0.432
MOD_GlcNHglycan	595	598	PF01048	0.239
MOD_GlcNHglycan	645	648	PF01048	0.628
MOD_GlcNHglycan	651	654	PF01048	0.652
MOD_GlcNHglycan	67	70	PF01048	0.580
MOD_GlcNHglycan	95	98	PF01048	0.707
MOD_GSK3_1	108	115	PF00069	0.647
MOD_GSK3_1	134	141	PF00069	0.510
MOD_GSK3_1	144	151	PF00069	0.546
MOD_GSK3_1	226	233	PF00069	0.570
MOD_GSK3_1	27	34	PF00069	0.730
MOD_GSK3_1	37	44	PF00069	0.693
MOD_GSK3_1	521	528	PF00069	0.250
MOD_GSK3_1	529	536	PF00069	0.223
MOD_GSK3_1	549	556	PF00069	0.109
MOD_GSK3_1	602	609	PF00069	0.267
MOD_GSK3_1	639	646	PF00069	0.471
MOD_GSK3_1	91	98	PF00069	0.739
MOD_N-GLC_1	31	36	PF02516	0.704
MOD_N-GLC_1	37	42	PF02516	0.700
MOD_N-GLC_2	102	104	PF02516	0.701
MOD_NEK2_1	113	118	PF00069	0.709
MOD_NEK2_1	221	226	PF00069	0.425
MOD_NEK2_1	27	32	PF00069	0.508
MOD_NEK2_1	309	314	PF00069	0.398
MOD_NEK2_1	434	439	PF00069	0.248
MOD_NEK2_1	510	515	PF00069	0.332
MOD_NEK2_1	547	552	PF00069	0.250
MOD_NEK2_1	639	644	PF00069	0.449
MOD_PIKK_1	249	255	PF00454	0.501
MOD_PIKK_1	530	536	PF00454	0.239
MOD_PIKK_1	580	586	PF00454	0.239
MOD_PIKK_1	631	637	PF00454	0.254
MOD_PKA_2	148	154	PF00069	0.547
MOD_PKA_2	301	307	PF00069	0.479
MOD_PKA_2	553	559	PF00069	0.239
MOD_PKA_2	640	646	PF00069	0.469
MOD_PKB_1	18	26	PF00069	0.649
MOD_PKB_1	277	285	PF00069	0.593
MOD_Plk_1	41	47	PF00069	0.583
MOD_Plk_2-3	498	504	PF00069	0.382
MOD_Plk_2-3	525	531	PF00069	0.239
MOD_Plk_4	190	196	PF00069	0.441
MOD_Plk_4	230	236	PF00069	0.541
MOD_Plk_4	446	452	PF00069	0.346
MOD_Plk_4	498	504	PF00069	0.301
MOD_Plk_4	535	541	PF00069	0.239
MOD_ProDKin_1	44	50	PF00069	0.677
MOD_ProDKin_1	478	484	PF00069	0.382
MOD_ProDKin_1	549	555	PF00069	0.239
MOD_ProDKin_1	602	608	PF00069	0.232
MOD_ProDKin_1	660	666	PF00069	0.709
MOD_ProDKin_1	670	676	PF00069	0.714
MOD_ProDKin_1	86	92	PF00069	0.701
MOD_SUMO_for_1	142	145	PF00179	0.496
MOD_SUMO_rev_2	322	330	PF00179	0.344
MOD_SUMO_rev_2	481	491	PF00179	0.375
MOD_SUMO_rev_2	498	508	PF00179	0.159
MOD_SUMO_rev_2	609	615	PF00179	0.239
TRG_DiLeu_BaEn_2	325	331	PF01217	0.357
TRG_DiLeu_BaEn_3	610	616	PF01217	0.266
TRG_DiLeu_BaEn_4	256	262	PF01217	0.429
TRG_DiLeu_BaLyEn_6	460	465	PF01217	0.254
TRG_ENDOCYTIC_2	133	136	PF00928	0.515
TRG_ENDOCYTIC_2	357	360	PF00928	0.239
TRG_ENDOCYTIC_2	425	428	PF00928	0.273
TRG_ENDOCYTIC_2	454	457	PF00928	0.332
TRG_ENDOCYTIC_2	539	542	PF00928	0.271
TRG_ENDOCYTIC_2	569	572	PF00928	0.239
TRG_ER_diArg_1	123	125	PF00400	0.659
TRG_ER_diArg_1	211	213	PF00400	0.499
TRG_ER_diArg_1	334	336	PF00400	0.369
TRG_ER_diArg_1	585	587	PF00400	0.262
TRG_ER_diArg_1	73	76	PF00400	0.631
TRG_Pf-PMV_PEXEL_1	212	216	PF00026	0.509
TRG_Pf-PMV_PEXEL_1	585	589	PF00026	0.289

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBJ1	Leptomonas seymouri	78%	99%
A0A1X0NJ50	Trypanosomatidae	59%	100%
A0A3S5IR34	Trypanosoma rangeli	56%	100%
A0A3S7X4W6	Leishmania donovani	97%	100%
A4HJS4	Leishmania braziliensis	92%	100%
A4I790	Leishmania infantum	97%	100%
C9ZMG9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
C9ZWL6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9B285	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O55047	Mus musculus	38%	94%
Q08CW1	Xenopus tropicalis	36%	97%
Q1ECX4	Danio rerio	36%	97%
Q39238	Arabidopsis thaliana	41%	98%
Q86UE8	Homo sapiens	37%	88%
Q8C0V0	Mus musculus	37%	88%
Q90ZY6	Danio rerio	38%	89%
Q9UKI8	Homo sapiens	37%	88%
V5B7L8	Trypanosoma cruzi	61%	100%