Lem3 cdc50 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Species:

Leishmania major

UniProt:

Q4Q5N2_LEIMA

TriTrypDb:

LmjF.32.0510 , LMJLV39_320010500 , LMJSD75_320010500

Length:

363

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 20
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	3
GO:0005794	Golgi apparatus	5	3
GO:0005886	plasma membrane	3	3
GO:0016020	membrane	2	21
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	21

Expansion

Sequence features

Q4Q5N2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5N2

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	21
GO:0051179	localization	1	21

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	124	128	PF00082	0.513
CLV_PCSK_SKI1_1	207	211	PF00082	0.553
CLV_PCSK_SKI1_1	97	101	PF00082	0.504
DOC_CYCLIN_yCln2_LP_2	106	112	PF00134	0.286
DOC_CYCLIN_yCln2_LP_2	145	151	PF00134	0.309
DOC_MAPK_FxFP_2	149	152	PF00069	0.306
DOC_MAPK_gen_1	284	293	PF00069	0.327
DOC_MAPK_MEF2A_6	284	293	PF00069	0.293
DOC_PP1_RVXF_1	56	62	PF00149	0.283
DOC_PP1_RVXF_1	95	101	PF00149	0.316
DOC_PP2B_LxvP_1	106	109	PF13499	0.315
DOC_PP2B_LxvP_1	145	148	PF13499	0.294
DOC_PP4_FxxP_1	149	152	PF00568	0.273
DOC_USP7_MATH_1	214	218	PF00917	0.327
DOC_USP7_MATH_1	277	281	PF00917	0.343
DOC_USP7_MATH_1	310	314	PF00917	0.365
DOC_USP7_MATH_1	320	324	PF00917	0.325
DOC_USP7_UBL2_3	7	11	PF12436	0.704
DOC_WW_Pin1_4	114	119	PF00397	0.328
DOC_WW_Pin1_4	24	29	PF00397	0.572
LIG_14-3-3_CanoR_1	124	133	PF00244	0.324
LIG_14-3-3_CanoR_1	97	103	PF00244	0.302
LIG_BIR_II_1	1	5	PF00653	0.636
LIG_BRCT_BRCA1_1	31	35	PF00533	0.411
LIG_BRCT_BRCA1_1	312	316	PF00533	0.335
LIG_BRCT_BRCA1_1	67	71	PF00533	0.437
LIG_FHA_1	105	111	PF00498	0.321
LIG_FHA_1	125	131	PF00498	0.352
LIG_FHA_1	277	283	PF00498	0.319
LIG_FHA_1	54	60	PF00498	0.423
LIG_FHA_2	264	270	PF00498	0.285
LIG_FHA_2	55	61	PF00498	0.318
LIG_LIR_Apic_2	161	166	PF02991	0.347
LIG_LIR_Gen_1	313	320	PF02991	0.314
LIG_LIR_Gen_1	60	71	PF02991	0.359
LIG_LIR_Nem_3	117	123	PF02991	0.307
LIG_LIR_Nem_3	129	135	PF02991	0.346
LIG_LIR_Nem_3	283	289	PF02991	0.251
LIG_LIR_Nem_3	297	302	PF02991	0.275
LIG_LIR_Nem_3	313	319	PF02991	0.201
LIG_LIR_Nem_3	32	38	PF02991	0.361
LIG_LIR_Nem_3	60	66	PF02991	0.304
LIG_LIR_Nem_3	68	72	PF02991	0.321
LIG_NRBOX	331	337	PF00104	0.450
LIG_PAM2_1	27	39	PF00658	0.379
LIG_PCNA_yPIPBox_3	322	336	PF02747	0.315
LIG_PTAP_UEV_1	306	311	PF05743	0.241
LIG_PTB_Apo_2	119	126	PF02174	0.368
LIG_PTB_Phospho_1	119	125	PF10480	0.380
LIG_SH2_CRK	223	227	PF00017	0.343
LIG_SH2_CRK	26	30	PF00017	0.371
LIG_SH2_CRK	286	290	PF00017	0.293
LIG_SH2_CRK	69	73	PF00017	0.305
LIG_SH2_GRB2like	163	166	PF00017	0.272
LIG_SH2_NCK_1	63	67	PF00017	0.337
LIG_SH2_PTP2	132	135	PF00017	0.179
LIG_SH2_SRC	63	66	PF00017	0.319
LIG_SH2_STAP1	79	83	PF00017	0.315
LIG_SH2_STAT3	311	314	PF00017	0.194
LIG_SH2_STAT5	125	128	PF00017	0.308
LIG_SH2_STAT5	132	135	PF00017	0.307
LIG_SH2_STAT5	248	251	PF00017	0.336
LIG_SH2_STAT5	299	302	PF00017	0.275
LIG_SH3_3	2	8	PF00018	0.715
LIG_SH3_3	288	294	PF00018	0.321
LIG_SH3_3	304	310	PF00018	0.272
LIG_SH3_3	78	84	PF00018	0.376
LIG_SH3_4	7	14	PF00018	0.609
LIG_SH3_5	294	298	PF00018	0.339
LIG_SUMO_SIM_anti_2	334	340	PF11976	0.477
LIG_SUMO_SIM_par_1	27	32	PF11976	0.565
LIG_SUMO_SIM_par_1	344	349	PF11976	0.312
LIG_SUMO_SIM_par_1	46	52	PF11976	0.329
LIG_SxIP_EBH_1	222	235	PF03271	0.179
LIG_TRFH_1	114	118	PF08558	0.440
LIG_TYR_ITIM	130	135	PF00017	0.444
LIG_UBA3_1	350	354	PF00899	0.400
MOD_CK1_1	140	146	PF00069	0.387
MOD_CK1_1	193	199	PF00069	0.391
MOD_CK1_1	27	33	PF00069	0.325
MOD_CK1_1	280	286	PF00069	0.316
MOD_CK2_1	262	268	PF00069	0.380
MOD_GlcNHglycan	135	138	PF01048	0.393
MOD_GlcNHglycan	139	142	PF01048	0.391
MOD_GlcNHglycan	160	163	PF01048	0.473
MOD_GlcNHglycan	191	195	PF01048	0.396
MOD_GlcNHglycan	210	213	PF01048	0.401
MOD_GlcNHglycan	236	239	PF01048	0.414
MOD_GlcNHglycan	242	245	PF01048	0.446
MOD_GlcNHglycan	31	34	PF01048	0.380
MOD_GlcNHglycan	51	54	PF01048	0.480
MOD_GlcNHglycan	91	94	PF01048	0.442
MOD_GSK3_1	133	140	PF00069	0.335
MOD_GSK3_1	193	200	PF00069	0.354
MOD_GSK3_1	276	283	PF00069	0.415
MOD_GSK3_1	49	56	PF00069	0.508
MOD_GSK3_1	85	92	PF00069	0.373
MOD_GSK3_1	98	105	PF00069	0.380
MOD_N-GLC_1	175	180	PF02516	0.321
MOD_N-GLC_1	197	202	PF02516	0.389
MOD_N-GLC_1	240	245	PF02516	0.403
MOD_N-GLC_1	85	90	PF02516	0.300
MOD_N-GLC_2	206	208	PF02516	0.287
MOD_NEK2_1	175	180	PF00069	0.318
MOD_NEK2_1	29	34	PF00069	0.406
MOD_NEK2_1	85	90	PF00069	0.347
MOD_NEK2_1	98	103	PF00069	0.393
MOD_PIKK_1	100	106	PF00454	0.451
MOD_PIKK_1	310	316	PF00454	0.439
MOD_PKA_2	137	143	PF00069	0.388
MOD_PKA_2	214	220	PF00069	0.451
MOD_PKB_1	222	230	PF00069	0.194
MOD_Plk_1	175	181	PF00069	0.326
MOD_Plk_4	184	190	PF00069	0.385
MOD_Plk_4	331	337	PF00069	0.421
MOD_Plk_4	346	352	PF00069	0.371
MOD_ProDKin_1	114	120	PF00069	0.403
MOD_ProDKin_1	24	30	PF00069	0.461
MOD_SUMO_rev_2	217	221	PF00179	0.374
TRG_ENDOCYTIC_2	123	126	PF00928	0.349
TRG_ENDOCYTIC_2	132	135	PF00928	0.356
TRG_ENDOCYTIC_2	26	29	PF00928	0.415
TRG_ENDOCYTIC_2	286	289	PF00928	0.348
TRG_ENDOCYTIC_2	299	302	PF00928	0.374
TRG_ENDOCYTIC_2	63	66	PF00928	0.351
TRG_ENDOCYTIC_2	69	72	PF00928	0.364
TRG_Pf-PMV_PEXEL_1	287	292	PF00026	0.424

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBG2	Leptomonas seymouri	32%	87%
A0A0N1PDQ9	Leptomonas seymouri	62%	100%
A0A0S4IMK4	Bodo saltans	32%	90%
A0A0S4J4T1	Bodo saltans	40%	96%
A0A0S4JB98	Bodo saltans	30%	90%
A0A0S4JJY0	Bodo saltans	30%	90%
A0A0S4JQR9	Bodo saltans	32%	69%
A0A1X0NHQ5	Trypanosomatidae	31%	76%
A0A1X0NV71	Trypanosomatidae	40%	96%
A0A1X0P5L3	Trypanosomatidae	32%	91%
A0A3R7LIN7	Trypanosoma rangeli	32%	85%
A0A3R7MGX3	Trypanosoma rangeli	43%	97%
A0A3S7X551	Leishmania donovani	91%	100%
A0A3S7X9N4	Leishmania donovani	31%	86%
A0A422NR22	Trypanosoma rangeli	34%	91%
A4HK31	Leishmania braziliensis	72%	100%
A4HN07	Leishmania braziliensis	30%	86%
A4I7N1	Leishmania infantum	91%	100%
A4IBN0	Leishmania infantum	31%	86%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	94%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	77%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AFG4	Leishmania major	31%	100%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	86%
H2L0H3	Caenorhabditis elegans	23%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	93%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	88%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	92%
Q17QL5	Bos taurus	31%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	92%
Q2T9P5	Bos taurus	24%	100%
Q3MIR4	Homo sapiens	27%	100%
Q5F362	Gallus gallus	31%	98%
Q5R6C0	Pongo abelii	30%	100%
Q67YS6	Arabidopsis thaliana	26%	100%
Q6AY41	Rattus norvegicus	30%	100%
Q8BHG3	Mus musculus	26%	100%
Q8L8W0	Arabidopsis thaliana	27%	100%
Q8VEK0	Mus musculus	31%	100%
Q95JK4	Macaca fascicularis	25%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	98%
Q9D4D7	Mus musculus	24%	100%
Q9LTW0	Arabidopsis thaliana	27%	100%
Q9NV96	Homo sapiens	30%	100%
Q9SA35	Arabidopsis thaliana	26%	100%
Q9SLK2	Arabidopsis thaliana	28%	100%
V5AY40	Trypanosoma cruzi	34%	100%
V5B6A6	Trypanosoma cruzi	42%	96%
V5BY23	Trypanosoma cruzi	32%	91%