Putative ubiquitin-conjugating enzyme protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme protein, putative

Species:

Leishmania major

UniProt:

Q4Q5I6_LEIMA

TriTrypDb:

LmjF.32.0960 , LMJLV39_320015400 , LMJSD75_320015400

Length:

271

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4Q5I6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5I6

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016740	transferase activity	2	11
GO:0016874	ligase activity	2	7
GO:0017076	purine nucleotide binding	4	12
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0061631	ubiquitin conjugating enzyme activity	5	3
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	3
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140096	catalytic activity, acting on a protein	2	3
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	25	29	PF00656	0.423
CLV_NRD_NRD_1	148	150	PF00675	0.285
CLV_PCSK_FUR_1	146	150	PF00082	0.449
CLV_PCSK_KEX2_1	148	150	PF00082	0.421
CLV_PCSK_SKI1_1	115	119	PF00082	0.337
CLV_PCSK_SKI1_1	12	16	PF00082	0.340
DEG_APCC_DBOX_1	114	122	PF00400	0.332
DEG_APCC_DBOX_1	259	267	PF00400	0.526
DEG_SCF_FBW7_1	106	113	PF00400	0.302
DOC_PP2B_LxvP_1	90	93	PF13499	0.302
DOC_PP4_FxxP_1	171	174	PF00568	0.468
DOC_PP4_FxxP_1	208	211	PF00568	0.653
DOC_PP4_FxxP_1	38	41	PF00568	0.291
DOC_PP4_FxxP_1	74	77	PF00568	0.349
DOC_PP4_MxPP_1	63	66	PF00568	0.302
DOC_USP7_MATH_1	128	132	PF00917	0.415
DOC_USP7_MATH_1	160	164	PF00917	0.423
DOC_USP7_MATH_1	213	217	PF00917	0.701
DOC_USP7_MATH_1	221	225	PF00917	0.619
DOC_WW_Pin1_4	106	111	PF00397	0.302
DOC_WW_Pin1_4	41	46	PF00397	0.302
LIG_14-3-3_CanoR_1	69	75	PF00244	0.388
LIG_AP_GAE_1	202	208	PF02883	0.701
LIG_BIR_II_1	1	5	PF00653	0.440
LIG_BRCT_BRCA1_1	53	57	PF00533	0.423
LIG_BRCT_BRCA1_2	53	59	PF00533	0.423
LIG_CSL_BTD_1	74	77	PF09270	0.302
LIG_EVH1_2	18	22	PF00568	0.318
LIG_FHA_1	31	37	PF00498	0.337
LIG_FHA_1	65	71	PF00498	0.397
LIG_FHA_2	37	43	PF00498	0.423
LIG_LIR_Apic_2	123	128	PF02991	0.423
LIG_LIR_Apic_2	170	174	PF02991	0.580
LIG_LIR_Apic_2	206	211	PF02991	0.651
LIG_LIR_Apic_2	72	77	PF02991	0.350
LIG_LIR_Gen_1	188	194	PF02991	0.699
LIG_LIR_Gen_1	202	213	PF02991	0.590
LIG_LIR_Gen_1	30	38	PF02991	0.449
LIG_LIR_Nem_3	10	14	PF02991	0.423
LIG_LIR_Nem_3	202	208	PF02991	0.699
LIG_LIR_Nem_3	30	35	PF02991	0.119
LIG_LIR_Nem_3	67	71	PF02991	0.360
LIG_NRBOX	251	257	PF00104	0.479
LIG_Pex14_1	34	38	PF04695	0.302
LIG_Pex14_2	57	61	PF04695	0.302
LIG_SH2_SRC	80	83	PF00017	0.360
LIG_SH2_STAP1	231	235	PF00017	0.508
LIG_SH3_1	59	65	PF00018	0.318
LIG_SH3_3	59	65	PF00018	0.391
LIG_SUMO_SIM_par_1	116	123	PF11976	0.303
LIG_UBA3_1	252	258	PF00899	0.444
LIG_WRC_WIRS_1	71	76	PF05994	0.360
MOD_CK1_1	100	106	PF00069	0.388
MOD_CK1_1	120	126	PF00069	0.302
MOD_CK1_1	30	36	PF00069	0.429
MOD_CK2_1	155	161	PF00069	0.437
MOD_CK2_1	36	42	PF00069	0.388
MOD_CMANNOS	34	37	PF00535	0.302
MOD_GlcNHglycan	1	4	PF01048	0.441
MOD_GlcNHglycan	215	218	PF01048	0.670
MOD_GSK3_1	106	113	PF00069	0.302
MOD_N-GLC_1	12	17	PF02516	0.423
MOD_NEK2_2	110	115	PF00069	0.337
MOD_Plk_1	100	106	PF00069	0.318
MOD_Plk_1	160	166	PF00069	0.543
MOD_Plk_1	206	212	PF00069	0.494
MOD_Plk_1	27	33	PF00069	0.388
MOD_Plk_2-3	97	103	PF00069	0.360
MOD_Plk_4	117	123	PF00069	0.321
MOD_Plk_4	262	268	PF00069	0.427
MOD_Plk_4	27	33	PF00069	0.423
MOD_Plk_4	70	76	PF00069	0.302
MOD_ProDKin_1	106	112	PF00069	0.302
MOD_ProDKin_1	41	47	PF00069	0.302
MOD_SUMO_rev_2	172	180	PF00179	0.616
TRG_DiLeu_BaEn_4	233	239	PF01217	0.542
TRG_ER_diArg_1	145	148	PF00400	0.414
TRG_NES_CRM1_1	112	127	PF08389	0.318
TRG_NES_CRM1_1	233	245	PF08389	0.449
TRG_Pf-PMV_PEXEL_1	12	17	PF00026	0.314
TRG_Pf-PMV_PEXEL_1	157	161	PF00026	0.426
TRG_Pf-PMV_PEXEL_1	162	166	PF00026	0.406

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDU3	Leptomonas seymouri	83%	100%
A0A0S4KIJ6	Bodo saltans	54%	100%
A0A1X0NV24	Trypanosomatidae	56%	100%
A0A3Q8IKW3	Leishmania donovani	97%	100%
A0A3R7LRA8	Trypanosoma rangeli	57%	84%
A4HK77	Leishmania braziliensis	93%	100%
A4I7R2	Leishmania infantum	97%	100%
D0A9W7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B2M0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
P14682	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	41%	92%
P49427	Homo sapiens	42%	100%
P52478	Caenorhabditis elegans	33%	100%
Q29503	Oryctolagus cuniculus	41%	100%
Q3B7D1	Rattus norvegicus	27%	76%
Q3UE37	Mus musculus	27%	76%
Q4QIK2	Leishmania major	33%	100%
Q66KB0	Xenopus tropicalis	26%	87%
Q6PCF7	Xenopus laevis	26%	87%
Q6ZWZ2	Mus musculus	41%	100%
Q712K3	Homo sapiens	41%	100%
Q8CFI2	Mus musculus	42%	100%
Q9H832	Homo sapiens	27%	77%
V5BGK9	Trypanosoma cruzi	60%	100%