mRNA guanylyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA capping enzyme, cytoplasmic

Species:

Leishmania major

UniProt:

Q4Q5E6_LEIMA

TriTrypDb:

LmjF.32.1360 , LMJLV39_320019700 , LMJSD75_320019700

Length:

692

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q5E6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5E6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006370	7-methylguanosine mRNA capping	8	9
GO:0006396	RNA processing	6	9
GO:0006397	mRNA processing	7	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009452	7-methylguanosine RNA capping	8	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0016071	mRNA metabolic process	6	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0036260	RNA capping	7	9
GO:0043170	macromolecule metabolic process	3	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0004484	mRNA guanylyltransferase activity	5	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0005525	GTP binding	5	5
GO:0008192	RNA guanylyltransferase activity	4	9
GO:0016740	transferase activity	2	9
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	9
GO:0016779	nucleotidyltransferase activity	4	9
GO:0017076	purine nucleotide binding	4	9
GO:0019001	guanyl nucleotide binding	5	5
GO:0019002	GMP binding	4	2
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0032561	guanyl ribonucleotide binding	5	5
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0070568	guanylyltransferase activity	5	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0003909	DNA ligase activity	4	1
GO:0003910	DNA ligase (ATP) activity	5	1
GO:0016874	ligase activity	2	1
GO:0016886	ligase activity, forming phosphoric ester bonds	3	1
GO:0140097	catalytic activity, acting on DNA	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	304	308	PF00656	0.460
CLV_NRD_NRD_1	131	133	PF00675	0.743
CLV_NRD_NRD_1	248	250	PF00675	0.551
CLV_NRD_NRD_1	296	298	PF00675	0.400
CLV_PCSK_KEX2_1	248	250	PF00082	0.419
CLV_PCSK_KEX2_1	296	298	PF00082	0.400
CLV_PCSK_KEX2_1	312	314	PF00082	0.444
CLV_PCSK_KEX2_1	382	384	PF00082	0.479
CLV_PCSK_KEX2_1	483	485	PF00082	0.348
CLV_PCSK_PC1ET2_1	312	314	PF00082	0.433
CLV_PCSK_PC1ET2_1	382	384	PF00082	0.483
CLV_PCSK_PC1ET2_1	483	485	PF00082	0.423
CLV_PCSK_PC7_1	378	384	PF00082	0.393
CLV_PCSK_SKI1_1	33	37	PF00082	0.548
CLV_PCSK_SKI1_1	480	484	PF00082	0.428
CLV_PCSK_SKI1_1	556	560	PF00082	0.363
CLV_PCSK_SKI1_1	668	672	PF00082	0.459
CLV_Separin_Metazoa	492	496	PF03568	0.296
DEG_APCC_DBOX_1	163	171	PF00400	0.452
DEG_SPOP_SBC_1	66	70	PF00917	0.580
DOC_CDC14_PxL_1	522	530	PF14671	0.296
DOC_CKS1_1	321	326	PF01111	0.396
DOC_CYCLIN_RxL_1	556	570	PF00134	0.366
DOC_CYCLIN_RxL_1	677	685	PF00134	0.395
DOC_CYCLIN_yCln2_LP_2	231	237	PF00134	0.289
DOC_CYCLIN_yCln2_LP_2	409	415	PF00134	0.296
DOC_CYCLIN_yCln2_LP_2	424	430	PF00134	0.265
DOC_MAPK_gen_1	199	208	PF00069	0.372
DOC_MAPK_gen_1	277	287	PF00069	0.495
DOC_MAPK_gen_1	312	319	PF00069	0.587
DOC_MAPK_gen_1	382	389	PF00069	0.296
DOC_MAPK_MEF2A_6	382	391	PF00069	0.296
DOC_MAPK_MEF2A_6	617	626	PF00069	0.296
DOC_MAPK_RevD_3	282	297	PF00069	0.390
DOC_PP1_RVXF_1	678	685	PF00149	0.424
DOC_PP2B_LxvP_1	231	234	PF13499	0.291
DOC_PP2B_LxvP_1	76	79	PF13499	0.509
DOC_PP4_FxxP_1	532	535	PF00568	0.174
DOC_PP4_FxxP_1	684	687	PF00568	0.412
DOC_PP4_MxPP_1	35	38	PF00568	0.523
DOC_USP7_MATH_1	10	14	PF00917	0.624
DOC_USP7_MATH_1	115	119	PF00917	0.481
DOC_USP7_MATH_1	136	140	PF00917	0.603
DOC_USP7_MATH_1	311	315	PF00917	0.587
DOC_USP7_MATH_1	362	366	PF00917	0.451
DOC_USP7_MATH_1	453	457	PF00917	0.250
DOC_USP7_MATH_1	459	463	PF00917	0.218
DOC_USP7_MATH_1	62	66	PF00917	0.639
DOC_USP7_MATH_1	687	691	PF00917	0.463
DOC_USP7_MATH_1	99	103	PF00917	0.573
DOC_USP7_UBL2_3	556	560	PF12436	0.267
DOC_WW_Pin1_4	105	110	PF00397	0.642
DOC_WW_Pin1_4	23	28	PF00397	0.585
DOC_WW_Pin1_4	320	325	PF00397	0.389
DOC_WW_Pin1_4	482	487	PF00397	0.296
DOC_WW_Pin1_4	58	63	PF00397	0.675
DOC_WW_Pin1_4	69	74	PF00397	0.574
DOC_WW_Pin1_4	81	86	PF00397	0.512
LIG_14-3-3_CanoR_1	383	388	PF00244	0.259
LIG_14-3-3_CanoR_1	617	621	PF00244	0.296
LIG_14-3-3_CanoR_1	654	664	PF00244	0.374
LIG_Actin_WH2_2	232	250	PF00022	0.382
LIG_Actin_WH2_2	602	619	PF00022	0.296
LIG_APCC_ABBA_1	603	608	PF00400	0.296
LIG_APCC_Cbox_2	209	215	PF00515	0.392
LIG_BIR_II_1	1	5	PF00653	0.689
LIG_BIR_III_3	1	5	PF00653	0.601
LIG_BRCT_BRCA1_1	430	434	PF00533	0.256
LIG_EH1_1	536	544	PF00400	0.296
LIG_eIF4E_1	328	334	PF01652	0.379
LIG_eIF4E_1	419	425	PF01652	0.267
LIG_FHA_1	342	348	PF00498	0.334
LIG_FHA_1	388	394	PF00498	0.278
LIG_FHA_1	639	645	PF00498	0.399
LIG_FHA_1	656	662	PF00498	0.245
LIG_FHA_2	154	160	PF00498	0.502
LIG_FHA_2	456	462	PF00498	0.204
LIG_FHA_2	582	588	PF00498	0.554
LIG_FHA_2	589	595	PF00498	0.396
LIG_FHA_2	669	675	PF00498	0.448
LIG_Integrin_RGD_1	253	255	PF01839	0.522
LIG_LIR_Apic_2	123	128	PF02991	0.708
LIG_LIR_Apic_2	26	31	PF02991	0.575
LIG_LIR_Gen_1	163	170	PF02991	0.452
LIG_LIR_Gen_1	300	311	PF02991	0.464
LIG_LIR_Gen_1	329	336	PF02991	0.439
LIG_LIR_Gen_1	338	347	PF02991	0.352
LIG_LIR_Gen_1	385	393	PF02991	0.383
LIG_LIR_Gen_1	420	429	PF02991	0.330
LIG_LIR_Gen_1	431	439	PF02991	0.204
LIG_LIR_Gen_1	473	482	PF02991	0.262
LIG_LIR_Gen_1	489	498	PF02991	0.399
LIG_LIR_Gen_1	584	592	PF02991	0.395
LIG_LIR_Gen_1	671	676	PF02991	0.442
LIG_LIR_Nem_3	163	168	PF02991	0.435
LIG_LIR_Nem_3	200	206	PF02991	0.413
LIG_LIR_Nem_3	261	266	PF02991	0.547
LIG_LIR_Nem_3	276	282	PF02991	0.270
LIG_LIR_Nem_3	300	306	PF02991	0.335
LIG_LIR_Nem_3	329	334	PF02991	0.432
LIG_LIR_Nem_3	338	343	PF02991	0.361
LIG_LIR_Nem_3	385	391	PF02991	0.383
LIG_LIR_Nem_3	404	409	PF02991	0.172
LIG_LIR_Nem_3	420	424	PF02991	0.188
LIG_LIR_Nem_3	431	437	PF02991	0.458
LIG_LIR_Nem_3	473	477	PF02991	0.262
LIG_LIR_Nem_3	485	491	PF02991	0.344
LIG_LIR_Nem_3	496	500	PF02991	0.308
LIG_LIR_Nem_3	584	589	PF02991	0.466
LIG_LIR_Nem_3	671	675	PF02991	0.448
LIG_LRP6_Inhibitor_1	36	49	PF00058	0.500
LIG_MYND_1	526	530	PF01753	0.296
LIG_PCNA_APIM_2	554	560	PF02747	0.296
LIG_PCNA_TLS_4	551	558	PF02747	0.296
LIG_Pex14_2	411	415	PF04695	0.250
LIG_Pex14_2	421	425	PF04695	0.250
LIG_Pex14_2	430	434	PF04695	0.144
LIG_Pex14_2	557	561	PF04695	0.267
LIG_Rb_LxCxE_1	524	540	PF01857	0.220
LIG_REV1ctd_RIR_1	485	494	PF16727	0.383
LIG_SH2_CRK	165	169	PF00017	0.432
LIG_SH2_CRK	203	207	PF00017	0.329
LIG_SH2_CRK	279	283	PF00017	0.340
LIG_SH2_CRK	511	515	PF00017	0.250
LIG_SH2_NCK_1	303	307	PF00017	0.328
LIG_SH2_PTP2	388	391	PF00017	0.296
LIG_SH2_PTP2	519	522	PF00017	0.267
LIG_SH2_SRC	369	372	PF00017	0.363
LIG_SH2_SRC	519	522	PF00017	0.250
LIG_SH2_STAP1	16	20	PF00017	0.726
LIG_SH2_STAP1	419	423	PF00017	0.296
LIG_SH2_STAP1	511	515	PF00017	0.250
LIG_SH2_STAT3	220	223	PF00017	0.414
LIG_SH2_STAT3	265	268	PF00017	0.420
LIG_SH2_STAT5	221	224	PF00017	0.282
LIG_SH2_STAT5	265	268	PF00017	0.591
LIG_SH2_STAT5	369	372	PF00017	0.358
LIG_SH2_STAT5	388	391	PF00017	0.355
LIG_SH2_STAT5	398	401	PF00017	0.228
LIG_SH2_STAT5	474	477	PF00017	0.265
LIG_SH2_STAT5	519	522	PF00017	0.250
LIG_SH3_1	318	324	PF00018	0.352
LIG_SH3_3	318	324	PF00018	0.422
LIG_SH3_3	45	51	PF00018	0.702
LIG_SH3_3	594	600	PF00018	0.369
LIG_SH3_3	647	653	PF00018	0.342
LIG_SUMO_SIM_par_1	619	625	PF11976	0.386
LIG_TRAF2_1	671	674	PF00917	0.367
LIG_TYR_ITIM	670	675	PF00017	0.444
LIG_UBA3_1	305	312	PF00899	0.490
MOD_CDK_SPxxK_3	105	112	PF00069	0.554
MOD_CK1_1	102	108	PF00069	0.733
MOD_CK1_1	152	158	PF00069	0.523
MOD_CK1_1	355	361	PF00069	0.593
MOD_CK1_1	65	71	PF00069	0.723
MOD_CK2_1	153	159	PF00069	0.505
MOD_CK2_1	455	461	PF00069	0.435
MOD_CK2_1	588	594	PF00069	0.523
MOD_CK2_1	656	662	PF00069	0.369
MOD_CK2_1	668	674	PF00069	0.370
MOD_Cter_Amidation	130	133	PF01082	0.577
MOD_GlcNHglycan	12	15	PF01048	0.624
MOD_GlcNHglycan	122	125	PF01048	0.739
MOD_GlcNHglycan	151	154	PF01048	0.390
MOD_GlcNHglycan	16	19	PF01048	0.583
MOD_GlcNHglycan	171	174	PF01048	0.251
MOD_GlcNHglycan	354	357	PF01048	0.507
MOD_GlcNHglycan	359	362	PF01048	0.407
MOD_GlcNHglycan	504	507	PF01048	0.391
MOD_GlcNHglycan	64	67	PF01048	0.614
MOD_GlcNHglycan	684	687	PF01048	0.367
MOD_GlcNHglycan	69	72	PF01048	0.607
MOD_GlcNHglycan	96	99	PF01048	0.707
MOD_GSK3_1	10	17	PF00069	0.641
MOD_GSK3_1	149	156	PF00069	0.455
MOD_GSK3_1	351	358	PF00069	0.505
MOD_GSK3_1	383	390	PF00069	0.285
MOD_GSK3_1	455	462	PF00069	0.300
MOD_GSK3_1	58	65	PF00069	0.699
MOD_GSK3_1	656	663	PF00069	0.484
MOD_GSK3_1	99	106	PF00069	0.694
MOD_N-GLC_1	352	357	PF02516	0.494
MOD_N-GLC_1	655	660	PF02516	0.265
MOD_N-GLC_1	687	692	PF02516	0.524
MOD_N-GLC_2	227	229	PF02516	0.316
MOD_NEK2_1	104	109	PF00069	0.730
MOD_NEK2_1	168	173	PF00069	0.423
MOD_NEK2_1	387	392	PF00069	0.280
MOD_NEK2_1	413	418	PF00069	0.296
MOD_NEK2_1	428	433	PF00069	0.199
MOD_NEK2_1	455	460	PF00069	0.363
MOD_NEK2_1	616	621	PF00069	0.296
MOD_NEK2_1	682	687	PF00069	0.412
MOD_PIKK_1	153	159	PF00454	0.420
MOD_PIKK_1	638	644	PF00454	0.430
MOD_PK_1	301	307	PF00069	0.359
MOD_PKA_1	382	388	PF00069	0.296
MOD_PKA_2	149	155	PF00069	0.515
MOD_PKA_2	382	388	PF00069	0.296
MOD_PKA_2	616	622	PF00069	0.296
MOD_PKA_2	682	688	PF00069	0.424
MOD_Plk_1	199	205	PF00069	0.597
MOD_Plk_1	655	661	PF00069	0.422
MOD_Plk_2-3	581	587	PF00069	0.556
MOD_Plk_4	301	307	PF00069	0.466
MOD_Plk_4	383	389	PF00069	0.296
MOD_Plk_4	545	551	PF00069	0.296
MOD_Plk_4	581	587	PF00069	0.400
MOD_Plk_4	616	622	PF00069	0.338
MOD_Plk_4	656	662	PF00069	0.284
MOD_ProDKin_1	105	111	PF00069	0.641
MOD_ProDKin_1	23	29	PF00069	0.586
MOD_ProDKin_1	320	326	PF00069	0.397
MOD_ProDKin_1	482	488	PF00069	0.296
MOD_ProDKin_1	58	64	PF00069	0.669
MOD_ProDKin_1	69	75	PF00069	0.556
MOD_ProDKin_1	81	87	PF00069	0.510
MOD_SUMO_rev_2	172	182	PF00179	0.451
MOD_SUMO_rev_2	252	258	PF00179	0.523
MOD_SUMO_rev_2	374	384	PF00179	0.388
TRG_DiLeu_BaEn_1	163	168	PF01217	0.452
TRG_DiLeu_BaEn_1	489	494	PF01217	0.296
TRG_DiLeu_BaEn_1	496	501	PF01217	0.243
TRG_DiLeu_BaEn_1	524	529	PF01217	0.250
TRG_DiLeu_BaLyEn_6	138	143	PF01217	0.385
TRG_DiLeu_BaLyEn_6	464	469	PF01217	0.296
TRG_ENDOCYTIC_2	165	168	PF00928	0.432
TRG_ENDOCYTIC_2	203	206	PF00928	0.335
TRG_ENDOCYTIC_2	279	282	PF00928	0.345
TRG_ENDOCYTIC_2	303	306	PF00928	0.544
TRG_ENDOCYTIC_2	328	331	PF00928	0.556
TRG_ENDOCYTIC_2	388	391	PF00928	0.407
TRG_ENDOCYTIC_2	398	401	PF00928	0.235
TRG_ENDOCYTIC_2	474	477	PF00928	0.326
TRG_ENDOCYTIC_2	491	494	PF00928	0.326
TRG_ENDOCYTIC_2	511	514	PF00928	0.144
TRG_ENDOCYTIC_2	519	522	PF00928	0.242
TRG_ENDOCYTIC_2	672	675	PF00928	0.403
TRG_ER_diArg_1	247	249	PF00400	0.564
TRG_ER_diArg_1	296	298	PF00400	0.400
TRG_ER_diArg_1	647	650	PF00400	0.296
TRG_NES_CRM1_1	230	245	PF08389	0.364
TRG_NES_CRM1_1	615	628	PF08389	0.265
TRG_NLS_Bipartite_1	296	316	PF00514	0.393
TRG_NLS_MonoExtN_4	311	316	PF00514	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IM27	Bodo saltans	33%	96%
A0A1X0NUI5	Trypanosomatidae	39%	100%
A0A3S7X5E2	Leishmania donovani	97%	100%
A0A422P1D1	Trypanosoma rangeli	40%	100%
A4HKC4	Leishmania braziliensis	85%	100%
D0AA12	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9B2R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5DHN7	Trypanosoma cruzi	42%	100%