PPM-type phosphatase domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein phosphatase 2C, putative

Species:

Leishmania major

UniProt:

Q4Q5B1_LEIMA

TriTrypDb:

LmjF.32.1690 , LMJLV39_320023100 , LMJSD75_320023100

Length:

563

Annotations

LeishMANIAdb annotations

This large family encompasses many diverse protein phosphatases. Some appear to have evolved transmembrane segments. Very tentatively they might regulate transmembrane receptor kinases.. The TM and non-TM groups diverged early in Eukaryota and appear to be distinct enough that they probably should not be part of the same cluster. This latter group has not expanded.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 1
Silverman et al.	no	yes: 1
Pissara et al.	no	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0016020	membrane	2	5
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	7
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

Q4Q5B1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5B1

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	13
GO:0006793	phosphorus metabolic process	3	13
GO:0006796	phosphate-containing compound metabolic process	4	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016311	dephosphorylation	5	13
GO:0019538	protein metabolic process	3	13
GO:0036211	protein modification process	4	13
GO:0043170	macromolecule metabolic process	3	13
GO:0043412	macromolecule modification	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0071704	organic substance metabolic process	2	13
GO:1901564	organonitrogen compound metabolic process	3	13
GO:0035970	peptidyl-threonine dephosphorylation	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	18
GO:0004721	phosphoprotein phosphatase activity	3	18
GO:0004722	protein serine/threonine phosphatase activity	4	18
GO:0016787	hydrolase activity	2	18
GO:0016788	hydrolase activity, acting on ester bonds	3	18
GO:0016791	phosphatase activity	5	18
GO:0042578	phosphoric ester hydrolase activity	4	18
GO:0140096	catalytic activity, acting on a protein	2	18
GO:0005488	binding	1	5
GO:0043167	ion binding	2	5
GO:0043169	cation binding	3	5
GO:0046872	metal ion binding	4	5
GO:0017018	myosin phosphatase activity	5	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	366	370	PF00656	0.284
CLV_NRD_NRD_1	10	12	PF00675	0.577
CLV_NRD_NRD_1	112	114	PF00675	0.422
CLV_NRD_NRD_1	145	147	PF00675	0.279
CLV_NRD_NRD_1	281	283	PF00675	0.279
CLV_NRD_NRD_1	45	47	PF00675	0.484
CLV_NRD_NRD_1	473	475	PF00675	0.538
CLV_PCSK_FUR_1	43	47	PF00082	0.441
CLV_PCSK_KEX2_1	10	12	PF00082	0.593
CLV_PCSK_KEX2_1	112	114	PF00082	0.429
CLV_PCSK_KEX2_1	145	147	PF00082	0.279
CLV_PCSK_KEX2_1	281	283	PF00082	0.283
CLV_PCSK_KEX2_1	392	394	PF00082	0.421
CLV_PCSK_KEX2_1	45	47	PF00082	0.439
CLV_PCSK_KEX2_1	5	7	PF00082	0.586
CLV_PCSK_PC1ET2_1	392	394	PF00082	0.421
CLV_PCSK_PC1ET2_1	5	7	PF00082	0.570
CLV_PCSK_PC7_1	6	12	PF00082	0.497
CLV_PCSK_SKI1_1	261	265	PF00082	0.472
CLV_PCSK_SKI1_1	504	508	PF00082	0.555
CLV_PCSK_SKI1_1	51	55	PF00082	0.581
CLV_Separin_Metazoa	109	113	PF03568	0.400
DEG_APCC_DBOX_1	356	364	PF00400	0.279
DEG_APCC_DBOX_1	50	58	PF00400	0.372
DOC_CKS1_1	451	456	PF01111	0.519
DOC_MAPK_gen_1	281	289	PF00069	0.279
DOC_MAPK_gen_1	351	360	PF00069	0.300
DOC_MAPK_MEF2A_6	51	59	PF00069	0.368
DOC_PP4_FxxP_1	349	352	PF00568	0.279
DOC_USP7_MATH_1	240	244	PF00917	0.279
DOC_USP7_MATH_1	310	314	PF00917	0.250
DOC_USP7_MATH_1	418	422	PF00917	0.588
DOC_USP7_MATH_1	491	495	PF00917	0.772
DOC_USP7_MATH_1	502	506	PF00917	0.550
DOC_USP7_MATH_1	510	514	PF00917	0.602
DOC_USP7_MATH_1	523	527	PF00917	0.568
DOC_USP7_MATH_1	533	537	PF00917	0.508
DOC_USP7_MATH_1	538	542	PF00917	0.523
DOC_USP7_MATH_1	549	553	PF00917	0.545
DOC_USP7_UBL2_3	190	194	PF12436	0.250
DOC_WW_Pin1_4	25	30	PF00397	0.381
DOC_WW_Pin1_4	370	375	PF00397	0.331
DOC_WW_Pin1_4	406	411	PF00397	0.651
DOC_WW_Pin1_4	450	455	PF00397	0.604
DOC_WW_Pin1_4	483	488	PF00397	0.608
DOC_WW_Pin1_4	529	534	PF00397	0.525
DOC_WW_Pin1_4	545	550	PF00397	0.571
LIG_14-3-3_CanoR_1	145	149	PF00244	0.285
LIG_14-3-3_CanoR_1	45	49	PF00244	0.432
LIG_APCC_ABBA_1	226	231	PF00400	0.279
LIG_Clathr_ClatBox_1	154	158	PF01394	0.279
LIG_DCNL_PONY_1	1	4	PF03556	0.514
LIG_EH1_1	47	55	PF00400	0.353
LIG_FHA_1	151	157	PF00498	0.279
LIG_FHA_1	33	39	PF00498	0.460
LIG_FHA_1	45	51	PF00498	0.591
LIG_FHA_1	506	512	PF00498	0.699
LIG_FHA_1	75	81	PF00498	0.287
LIG_FHA_2	421	427	PF00498	0.600
LIG_LIR_Apic_2	348	352	PF02991	0.279
LIG_LIR_Gen_1	180	191	PF02991	0.250
LIG_LIR_Gen_1	203	212	PF02991	0.338
LIG_LIR_Gen_1	327	337	PF02991	0.387
LIG_LIR_Nem_3	130	136	PF02991	0.359
LIG_LIR_Nem_3	180	186	PF02991	0.250
LIG_LIR_Nem_3	203	208	PF02991	0.338
LIG_LIR_Nem_3	327	332	PF02991	0.387
LIG_LIR_Nem_3	63	68	PF02991	0.326
LIG_LIR_Nem_3	69	73	PF02991	0.320
LIG_PCNA_yPIPBox_3	273	287	PF02747	0.279
LIG_PCNA_yPIPBox_3	31	45	PF02747	0.443
LIG_SH2_CRK	70	74	PF00017	0.377
LIG_SH2_PTP2	480	483	PF00017	0.517
LIG_SH2_SRC	211	214	PF00017	0.271
LIG_SH2_SRC	229	232	PF00017	0.185
LIG_SH2_SRC	480	483	PF00017	0.517
LIG_SH2_STAT3	181	184	PF00017	0.279
LIG_SH2_STAT5	177	180	PF00017	0.307
LIG_SH2_STAT5	211	214	PF00017	0.285
LIG_SH2_STAT5	229	232	PF00017	0.346
LIG_SH2_STAT5	236	239	PF00017	0.242
LIG_SH2_STAT5	26	29	PF00017	0.440
LIG_SH2_STAT5	277	280	PF00017	0.296
LIG_SH2_STAT5	48	51	PF00017	0.417
LIG_SH2_STAT5	480	483	PF00017	0.517
LIG_SH3_1	6	12	PF00018	0.497
LIG_SH3_3	259	265	PF00018	0.334
LIG_SH3_3	412	418	PF00018	0.609
LIG_SH3_3	448	454	PF00018	0.489
LIG_SH3_3	543	549	PF00018	0.720
LIG_SH3_3	6	12	PF00018	0.531
LIG_SUMO_SIM_anti_2	153	158	PF11976	0.279
LIG_SUMO_SIM_anti_2	385	390	PF11976	0.387
LIG_SUMO_SIM_par_1	152	158	PF11976	0.279
LIG_SUMO_SIM_par_1	285	290	PF11976	0.279
LIG_SUMO_SIM_par_1	442	447	PF11976	0.505
LIG_UBA3_1	1	5	PF00899	0.515
LIG_UBA3_1	154	159	PF00899	0.279
LIG_WRC_WIRS_1	215	220	PF05994	0.264
LIG_WW_3	7	11	PF00397	0.530
MOD_CDC14_SPxK_1	548	551	PF00782	0.490
MOD_CDK_SPK_2	411	416	PF00069	0.499
MOD_CDK_SPxK_1	545	551	PF00069	0.498
MOD_CK1_1	214	220	PF00069	0.299
MOD_CK1_1	500	506	PF00069	0.631
MOD_CK1_1	513	519	PF00069	0.525
MOD_CK2_1	310	316	PF00069	0.319
MOD_GlcNHglycan	127	130	PF01048	0.394
MOD_GlcNHglycan	169	172	PF01048	0.312
MOD_GlcNHglycan	19	22	PF01048	0.485
MOD_GlcNHglycan	312	315	PF01048	0.274
MOD_GlcNHglycan	420	423	PF01048	0.550
MOD_GlcNHglycan	494	497	PF01048	0.621
MOD_GlcNHglycan	499	502	PF01048	0.625
MOD_GlcNHglycan	504	507	PF01048	0.721
MOD_GlcNHglycan	518	521	PF01048	0.535
MOD_GlcNHglycan	536	539	PF01048	0.758
MOD_GSK3_1	167	174	PF00069	0.340
MOD_GSK3_1	398	405	PF00069	0.567
MOD_GSK3_1	483	490	PF00069	0.627
MOD_GSK3_1	492	499	PF00069	0.538
MOD_GSK3_1	512	519	PF00069	0.572
MOD_GSK3_1	529	536	PF00069	0.616
MOD_GSK3_1	545	552	PF00069	0.704
MOD_N-GLC_1	249	254	PF02516	0.250
MOD_N-GLC_1	299	304	PF02516	0.234
MOD_N-GLC_1	382	387	PF02516	0.399
MOD_N-GLC_1	436	441	PF02516	0.619
MOD_N-GLC_1	533	538	PF02516	0.562
MOD_NEK2_1	287	292	PF00069	0.382
MOD_NEK2_1	444	449	PF00069	0.515
MOD_NEK2_1	511	516	PF00069	0.809
MOD_NEK2_1	55	60	PF00069	0.389
MOD_NEK2_2	538	543	PF00069	0.546
MOD_NEK2_2	554	559	PF00069	0.508
MOD_PIKK_1	299	305	PF00454	0.316
MOD_PIKK_1	436	442	PF00454	0.514
MOD_PK_1	249	255	PF00069	0.250
MOD_PKA_2	144	150	PF00069	0.367
MOD_PKA_2	317	323	PF00069	0.286
MOD_PKA_2	44	50	PF00069	0.466
MOD_PKA_2	511	517	PF00069	0.601
MOD_PKA_2	523	529	PF00069	0.606
MOD_Plk_1	299	305	PF00069	0.252
MOD_Plk_1	32	38	PF00069	0.364
MOD_Plk_1	382	388	PF00069	0.431
MOD_Plk_4	150	156	PF00069	0.329
MOD_Plk_4	382	388	PF00069	0.377
MOD_Plk_4	74	80	PF00069	0.301
MOD_ProDKin_1	25	31	PF00069	0.380
MOD_ProDKin_1	370	376	PF00069	0.331
MOD_ProDKin_1	406	412	PF00069	0.646
MOD_ProDKin_1	450	456	PF00069	0.608
MOD_ProDKin_1	483	489	PF00069	0.608
MOD_ProDKin_1	529	535	PF00069	0.524
MOD_ProDKin_1	545	551	PF00069	0.573
MOD_SUMO_for_1	193	196	PF00179	0.250
MOD_SUMO_rev_2	254	263	PF00179	0.383
MOD_SUMO_rev_2	476	486	PF00179	0.601
TRG_ENDOCYTIC_2	202	205	PF00928	0.279
TRG_ENDOCYTIC_2	70	73	PF00928	0.337
TRG_ER_diArg_1	111	113	PF00400	0.403
TRG_ER_diArg_1	144	146	PF00400	0.279
TRG_ER_diArg_1	281	283	PF00400	0.305
TRG_ER_diArg_1	350	353	PF00400	0.263
TRG_ER_diArg_1	9	11	PF00400	0.536
TRG_Pf-PMV_PEXEL_1	291	295	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	36	40	PF00026	0.492

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PET0	Leptomonas seymouri	68%	97%
A0A3Q8IFG7	Leishmania donovani	31%	100%
A0A3Q8IGS1	Leishmania donovani	92%	100%
A0A3Q8IL63	Leishmania donovani	29%	100%
A0A3S7WTA2	Leishmania donovani	36%	100%
A0A3S7WZ14	Leishmania donovani	32%	100%
A4H7Y6	Leishmania braziliensis	36%	100%
A4HAW5	Leishmania braziliensis	29%	100%
A4HE10	Leishmania braziliensis	32%	100%
A4HHY5	Leishmania braziliensis	29%	100%
A4HKF6	Leishmania braziliensis	77%	99%
A4HWB4	Leishmania infantum	36%	100%
A4I1B7	Leishmania infantum	32%	100%
A4I565	Leishmania infantum	31%	100%
A4I7Y4	Leishmania infantum	92%	100%
A4IA25	Leishmania infantum	29%	100%
E9AQ14	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AXF3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B0G2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B2U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9B540	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
Q4Q2U6	Leishmania major	29%	100%
Q4Q7S1	Leishmania major	31%	100%
Q4QA19	Leishmania major	33%	100%
Q4QFG7	Leishmania major	36%	100%
Q6K1U0	Oryza sativa subsp. japonica	29%	77%
Q6K1U4	Oryza sativa subsp. japonica	28%	100%
Q6K5I0	Oryza sativa subsp. japonica	31%	100%