DNA polymerase alpha subunit B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase alpha/epsilon subunit B, putative

Species:

Leishmania major

UniProt:

Q4Q5A8_LEIMA

TriTrypDb:

LmjF.32.1720 , LMJLV39_320023400 , LMJSD75_320023400

Length:

726

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	2
GO:0005658	alpha DNA polymerase:primase complex	4	2
GO:0030880	RNA polymerase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0042575	DNA polymerase complex	3	2
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	2
GO:0140513	nuclear protein-containing complex	2	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2
GO:0005634	nucleus	5	10
GO:0043226	organelle	2	10
GO:0043227	membrane-bounded organelle	3	10
GO:0043229	intracellular organelle	3	10
GO:0043231	intracellular membrane-bounded organelle	4	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4Q5A8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q5A8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006270	DNA replication initiation	5	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006260	DNA replication	5	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016779	nucleotidyltransferase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	111	115	PF00656	0.675
CLV_C14_Caspase3-7	326	330	PF00656	0.635
CLV_C14_Caspase3-7	559	563	PF00656	0.809
CLV_C14_Caspase3-7	611	615	PF00656	0.748
CLV_C14_Caspase3-7	658	662	PF00656	0.692
CLV_NRD_NRD_1	343	345	PF00675	0.509
CLV_NRD_NRD_1	492	494	PF00675	0.344
CLV_NRD_NRD_1	554	556	PF00675	0.687
CLV_NRD_NRD_1	707	709	PF00675	0.590
CLV_NRD_NRD_1	71	73	PF00675	0.509
CLV_PCSK_KEX2_1	343	345	PF00082	0.509
CLV_PCSK_KEX2_1	492	494	PF00082	0.344
CLV_PCSK_KEX2_1	554	556	PF00082	0.687
CLV_PCSK_KEX2_1	599	601	PF00082	0.639
CLV_PCSK_KEX2_1	707	709	PF00082	0.590
CLV_PCSK_KEX2_1	71	73	PF00082	0.506
CLV_PCSK_PC1ET2_1	599	601	PF00082	0.639
CLV_PCSK_SKI1_1	28	32	PF00082	0.721
CLV_PCSK_SKI1_1	283	287	PF00082	0.524
CLV_PCSK_SKI1_1	308	312	PF00082	0.697
CLV_PCSK_SKI1_1	351	355	PF00082	0.361
CLV_PCSK_SKI1_1	381	385	PF00082	0.385
CLV_PCSK_SKI1_1	413	417	PF00082	0.382
CLV_PCSK_SKI1_1	420	424	PF00082	0.296
CLV_PCSK_SKI1_1	599	603	PF00082	0.637
CLV_PCSK_SKI1_1	708	712	PF00082	0.593
CLV_PCSK_SKI1_1	79	83	PF00082	0.399
DEG_APCC_DBOX_1	282	290	PF00400	0.540
DEG_APCC_DBOX_1	380	388	PF00400	0.449
DEG_APCC_DBOX_1	412	420	PF00400	0.423
DEG_Nend_UBRbox_3	1	3	PF02207	0.680
DEG_SCF_FBW7_1	625	631	PF00400	0.752
DEG_SPOP_SBC_1	214	218	PF00917	0.749
DEG_SPOP_SBC_1	314	318	PF00917	0.648
DEG_SPOP_SBC_1	563	567	PF00917	0.689
DOC_ANK_TNKS_1	554	561	PF00023	0.688
DOC_CDC14_PxL_1	517	525	PF14671	0.550
DOC_CKS1_1	622	627	PF01111	0.749
DOC_CYCLIN_RxL_1	378	386	PF00134	0.364
DOC_CYCLIN_RxL_1	417	425	PF00134	0.449
DOC_CYCLIN_yCln2_LP_2	26	32	PF00134	0.662
DOC_MAPK_DCC_7	633	642	PF00069	0.586
DOC_MAPK_MEF2A_6	518	525	PF00069	0.555
DOC_MAPK_MEF2A_6	633	642	PF00069	0.586
DOC_MAPK_MEF2A_6	8	17	PF00069	0.583
DOC_MAPK_RevD_3	692	708	PF00069	0.558
DOC_PP1_RVXF_1	331	337	PF00149	0.443
DOC_PP2B_LxvP_1	30	33	PF13499	0.651
DOC_PP2B_LxvP_1	503	506	PF13499	0.363
DOC_PP4_FxxP_1	294	297	PF00568	0.403
DOC_PP4_FxxP_1	371	374	PF00568	0.449
DOC_USP7_MATH_1	126	130	PF00917	0.639
DOC_USP7_MATH_1	186	190	PF00917	0.650
DOC_USP7_MATH_1	203	207	PF00917	0.669
DOC_USP7_MATH_1	214	218	PF00917	0.701
DOC_USP7_MATH_1	221	225	PF00917	0.584
DOC_USP7_MATH_1	314	318	PF00917	0.653
DOC_USP7_MATH_1	325	329	PF00917	0.682
DOC_USP7_MATH_1	352	356	PF00917	0.242
DOC_USP7_MATH_1	374	378	PF00917	0.434
DOC_USP7_MATH_1	38	42	PF00917	0.651
DOC_USP7_MATH_1	487	491	PF00917	0.418
DOC_USP7_MATH_1	543	547	PF00917	0.683
DOC_USP7_MATH_1	558	562	PF00917	0.727
DOC_USP7_MATH_1	563	567	PF00917	0.711
DOC_USP7_MATH_1	570	574	PF00917	0.648
DOC_USP7_MATH_1	628	632	PF00917	0.710
DOC_WW_Pin1_4	621	626	PF00397	0.790
DOC_WW_Pin1_4	684	689	PF00397	0.659
LIG_14-3-3_CanoR_1	251	255	PF00244	0.411
LIG_14-3-3_CanoR_1	257	262	PF00244	0.452
LIG_14-3-3_CanoR_1	351	358	PF00244	0.418
LIG_14-3-3_CanoR_1	457	461	PF00244	0.363
LIG_14-3-3_CanoR_1	55	60	PF00244	0.580
LIG_14-3-3_CanoR_1	707	716	PF00244	0.567
LIG_AP2alpha_2	276	278	PF02296	0.539
LIG_BIR_III_4	114	118	PF00653	0.687
LIG_BIR_III_4	605	609	PF00653	0.724
LIG_BRCT_BRCA1_1	337	341	PF00533	0.431
LIG_BRCT_BRCA1_1	686	690	PF00533	0.656
LIG_Clathr_ClatBox_1	16	20	PF01394	0.737
LIG_Clathr_ClatBox_1	43	47	PF01394	0.668
LIG_DLG_GKlike_1	55	62	PF00625	0.619
LIG_eIF4E_1	410	416	PF01652	0.449
LIG_FHA_1	221	227	PF00498	0.682
LIG_FHA_1	285	291	PF00498	0.382
LIG_FHA_1	410	416	PF00498	0.449
LIG_FHA_1	444	450	PF00498	0.379
LIG_FHA_1	512	518	PF00498	0.547
LIG_FHA_1	632	638	PF00498	0.724
LIG_FHA_1	700	706	PF00498	0.622
LIG_FHA_1	711	717	PF00498	0.651
LIG_FHA_2	105	111	PF00498	0.712
LIG_FHA_2	388	394	PF00498	0.342
LIG_FHA_2	399	405	PF00498	0.328
LIG_FHA_2	548	554	PF00498	0.558
LIG_FHA_2	92	98	PF00498	0.705
LIG_Integrin_isoDGR_2	500	502	PF01839	0.475
LIG_Integrin_RGD_1	427	429	PF01839	0.398
LIG_LIR_Apic_2	293	297	PF02991	0.517
LIG_LIR_Gen_1	187	196	PF02991	0.668
LIG_LIR_Gen_1	390	399	PF02991	0.377
LIG_LIR_Gen_1	573	583	PF02991	0.672
LIG_LIR_LC3C_4	672	677	PF02991	0.538
LIG_LIR_Nem_3	159	163	PF02991	0.387
LIG_LIR_Nem_3	187	193	PF02991	0.659
LIG_LIR_Nem_3	260	265	PF02991	0.452
LIG_LIR_Nem_3	390	394	PF02991	0.427
LIG_LIR_Nem_3	56	62	PF02991	0.653
LIG_LIR_Nem_3	573	579	PF02991	0.673
LIG_MYND_1	29	33	PF01753	0.731
LIG_NRBOX	12	18	PF00104	0.602
LIG_PALB2_WD40_1	257	265	PF16756	0.488
LIG_PDZ_Class_2	721	726	PF00595	0.651
LIG_Pex14_1	576	580	PF04695	0.760
LIG_Pex14_2	371	375	PF04695	0.449
LIG_SH2_CRK	508	512	PF00017	0.359
LIG_SH2_CRK	59	63	PF00017	0.639
LIG_SH2_NCK_1	441	445	PF00017	0.449
LIG_SH2_PTP2	504	507	PF00017	0.398
LIG_SH2_SRC	441	444	PF00017	0.449
LIG_SH2_SRC	527	530	PF00017	0.641
LIG_SH2_SRC	636	639	PF00017	0.615
LIG_SH2_STAP1	255	259	PF00017	0.455
LIG_SH2_STAT3	255	258	PF00017	0.549
LIG_SH2_STAT3	77	80	PF00017	0.291
LIG_SH2_STAT5	161	164	PF00017	0.506
LIG_SH2_STAT5	172	175	PF00017	0.454
LIG_SH2_STAT5	255	258	PF00017	0.434
LIG_SH2_STAT5	504	507	PF00017	0.396
LIG_SH2_STAT5	527	530	PF00017	0.605
LIG_SH2_STAT5	59	62	PF00017	0.543
LIG_SH2_STAT5	636	639	PF00017	0.615
LIG_SH3_2	300	305	PF14604	0.635
LIG_SH3_3	116	122	PF00018	0.735
LIG_SH3_3	294	300	PF00018	0.405
LIG_SH3_3	303	309	PF00018	0.547
LIG_SH3_3	411	417	PF00018	0.409
LIG_SH3_3	431	437	PF00018	0.348
LIG_SH3_3	616	622	PF00018	0.807
LIG_SH3_3	694	700	PF00018	0.488
LIG_SUMO_SIM_anti_2	363	368	PF11976	0.361
LIG_SUMO_SIM_anti_2	672	678	PF11976	0.503
LIG_SUMO_SIM_par_1	40	47	PF11976	0.714
LIG_SUMO_SIM_par_1	420	425	PF11976	0.334
LIG_TRAF2_1	229	232	PF00917	0.706
LIG_TRAF2_1	240	243	PF00917	0.655
LIG_TRAF2_1	401	404	PF00917	0.364
LIG_TRAF2_1	94	97	PF00917	0.740
LIG_UBA3_1	415	420	PF00899	0.449
LIG_UBA3_1	534	538	PF00899	0.633
LIG_WRC_WIRS_1	395	400	PF05994	0.437
MOD_CDK_SPK_2	684	689	PF00069	0.642
MOD_CK1_1	147	153	PF00069	0.635
MOD_CK1_1	253	259	PF00069	0.419
MOD_CK1_1	260	266	PF00069	0.399
MOD_CK1_1	298	304	PF00069	0.531
MOD_CK1_1	37	43	PF00069	0.686
MOD_CK1_1	547	553	PF00069	0.777
MOD_CK1_1	561	567	PF00069	0.724
MOD_CK1_1	568	574	PF00069	0.680
MOD_CK1_1	626	632	PF00069	0.687
MOD_CK1_1	66	72	PF00069	0.515
MOD_CK1_1	667	673	PF00069	0.525
MOD_CK1_1	683	689	PF00069	0.608
MOD_CK2_1	104	110	PF00069	0.678
MOD_CK2_1	374	380	PF00069	0.379
MOD_CK2_1	387	393	PF00069	0.263
MOD_CK2_1	398	404	PF00069	0.344
MOD_CK2_1	527	533	PF00069	0.608
MOD_CK2_1	578	584	PF00069	0.776
MOD_CK2_1	629	635	PF00069	0.630
MOD_CK2_1	91	97	PF00069	0.664
MOD_GlcNHglycan	145	149	PF01048	0.617
MOD_GlcNHglycan	205	208	PF01048	0.717
MOD_GlcNHglycan	223	226	PF01048	0.486
MOD_GlcNHglycan	280	283	PF01048	0.499
MOD_GlcNHglycan	311	314	PF01048	0.634
MOD_GlcNHglycan	318	321	PF01048	0.637
MOD_GlcNHglycan	337	340	PF01048	0.337
MOD_GlcNHglycan	345	349	PF01048	0.378
MOD_GlcNHglycan	36	39	PF01048	0.647
MOD_GlcNHglycan	40	43	PF01048	0.650
MOD_GlcNHglycan	567	570	PF01048	0.707
MOD_GlcNHglycan	616	619	PF01048	0.787
MOD_GlcNHglycan	64	68	PF01048	0.599
MOD_GlcNHglycan	666	669	PF01048	0.586
MOD_GlcNHglycan	671	674	PF01048	0.493
MOD_GSK3_1	182	189	PF00069	0.647
MOD_GSK3_1	209	216	PF00069	0.656
MOD_GSK3_1	253	260	PF00069	0.500
MOD_GSK3_1	309	316	PF00069	0.592
MOD_GSK3_1	34	41	PF00069	0.686
MOD_GSK3_1	383	390	PF00069	0.347
MOD_GSK3_1	394	401	PF00069	0.312
MOD_GSK3_1	483	490	PF00069	0.348
MOD_GSK3_1	543	550	PF00069	0.699
MOD_GSK3_1	558	565	PF00069	0.671
MOD_GSK3_1	624	631	PF00069	0.685
MOD_GSK3_1	680	687	PF00069	0.620
MOD_GSK3_1	695	702	PF00069	0.378
MOD_GSK3_1	710	717	PF00069	0.526
MOD_GSK3_1	96	103	PF00069	0.741
MOD_NEK2_1	209	214	PF00069	0.735
MOD_NEK2_1	278	283	PF00069	0.467
MOD_NEK2_1	387	392	PF00069	0.362
MOD_NEK2_1	402	407	PF00069	0.320
MOD_NEK2_1	422	427	PF00069	0.441
MOD_NEK2_1	450	455	PF00069	0.449
MOD_NEK2_1	54	59	PF00069	0.554
MOD_NEK2_1	544	549	PF00069	0.537
MOD_NEK2_1	91	96	PF00069	0.640
MOD_NEK2_2	487	492	PF00069	0.449
MOD_NEK2_2	575	580	PF00069	0.550
MOD_PIKK_1	138	144	PF00454	0.380
MOD_PIKK_1	150	156	PF00454	0.572
MOD_PIKK_1	352	358	PF00454	0.369
MOD_PIKK_1	428	434	PF00454	0.328
MOD_PIKK_1	527	533	PF00454	0.661
MOD_PIKK_1	91	97	PF00454	0.693
MOD_PKA_2	100	106	PF00069	0.691
MOD_PKA_2	203	209	PF00069	0.678
MOD_PKA_2	214	220	PF00069	0.606
MOD_PKA_2	250	256	PF00069	0.400
MOD_PKA_2	298	304	PF00069	0.549
MOD_PKA_2	456	462	PF00069	0.363
MOD_PKA_2	48	54	PF00069	0.575
MOD_Plk_1	144	150	PF00069	0.603
MOD_Plk_1	186	192	PF00069	0.712
MOD_Plk_1	230	236	PF00069	0.495
MOD_Plk_1	402	408	PF00069	0.350
MOD_Plk_1	428	434	PF00069	0.315
MOD_Plk_1	695	701	PF00069	0.479
MOD_Plk_4	100	106	PF00069	0.689
MOD_Plk_4	250	256	PF00069	0.400
MOD_Plk_4	257	263	PF00069	0.430
MOD_Plk_4	394	400	PF00069	0.358
MOD_Plk_4	436	442	PF00069	0.340
MOD_Plk_4	575	581	PF00069	0.579
MOD_ProDKin_1	621	627	PF00069	0.790
MOD_ProDKin_1	684	690	PF00069	0.657
MOD_SUMO_for_1	105	108	PF00179	0.641
MOD_SUMO_for_1	601	604	PF00179	0.682
MOD_SUMO_for_1	688	691	PF00179	0.630
MOD_SUMO_rev_2	530	540	PF00179	0.676
TRG_DiLeu_BaEn_1	27	32	PF01217	0.662
TRG_DiLeu_BaLyEn_6	26	31	PF01217	0.704
TRG_DiLeu_BaLyEn_6	306	311	PF01217	0.717
TRG_DiLeu_BaLyEn_6	411	416	PF01217	0.449
TRG_ENDOCYTIC_2	504	507	PF00928	0.328
TRG_ENDOCYTIC_2	59	62	PF00928	0.638
TRG_ENDOCYTIC_2	78	81	PF00928	0.217
TRG_ER_diArg_1	342	344	PF00400	0.505
TRG_ER_diArg_1	491	493	PF00400	0.344
TRG_ER_diArg_1	70	72	PF00400	0.471
TRG_ER_diArg_1	706	708	PF00400	0.595
TRG_NLS_MonoExtC_3	598	604	PF00514	0.636
TRG_NLS_MonoExtN_4	597	603	PF00514	0.694

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAT5	Leptomonas seymouri	64%	100%
A0A0S4JBE4	Bodo saltans	31%	100%
A0A1X0NW10	Trypanosomatidae	42%	100%
A0A3Q8IL35	Leishmania donovani	91%	100%
A0A3R7NGI8	Trypanosoma rangeli	42%	100%
A4HKF9	Leishmania braziliensis	76%	100%
A4I7Y7	Leishmania infantum	91%	100%
D0AA49	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9B2U8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
V5DFV4	Trypanosoma cruzi	41%	100%