Putative 1-acyl-sn-glycerol-3-phosphateacyltransferase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

1-acyl-sn-glycerol-3-phosphateacyltransferase-like protein, putative

Species:

Leishmania major

UniProt:

Q4Q582_LEIMA

TriTrypDb:

LmjF.32.1960 , LMJLV39_320026100 , LMJSD75_320026100

Length:

304

Annotations

LeishMANIAdb annotations

This conserved lipid acyltransferase has many re-entrant segments but only one true TM helix. Most closely related to bacterial acyltransferases.. Heavily expanded in kinetoplastids for unknown reasons. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 36

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	14
GO:0110165	cellular anatomical entity	1	14

Expansion

Sequence features

Q4Q582

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q582

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	2
GO:0006644	phospholipid metabolic process	4	2
GO:0006650	glycerophospholipid metabolic process	5	2
GO:0006654	phosphatidic acid biosynthetic process	6	2
GO:0006793	phosphorus metabolic process	3	2
GO:0006796	phosphate-containing compound metabolic process	4	2
GO:0008152	metabolic process	1	2
GO:0008610	lipid biosynthetic process	4	2
GO:0008654	phospholipid biosynthetic process	5	2
GO:0009058	biosynthetic process	2	2
GO:0009987	cellular process	1	2
GO:0019637	organophosphate metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044255	cellular lipid metabolic process	3	2
GO:0045017	glycerolipid biosynthetic process	4	2
GO:0046473	phosphatidic acid metabolic process	6	2
GO:0046474	glycerophospholipid biosynthetic process	5	2
GO:0046486	glycerolipid metabolic process	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0090407	organophosphate biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	7	8
GO:0008374	O-acyltransferase activity	5	8
GO:0016411	acylglycerol O-acyltransferase activity	6	8
GO:0016740	transferase activity	2	16
GO:0016746	acyltransferase activity	3	16
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	8
GO:0042171	lysophosphatidic acid acyltransferase activity	6	8
GO:0071617	lysophospholipid acyltransferase activity	5	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	226	230	PF00656	0.326
CLV_NRD_NRD_1	144	146	PF00675	0.360
CLV_NRD_NRD_1	163	165	PF00675	0.417
CLV_NRD_NRD_1	167	169	PF00675	0.404
CLV_NRD_NRD_1	4	6	PF00675	0.334
CLV_PCSK_FUR_1	142	146	PF00082	0.360
CLV_PCSK_KEX2_1	144	146	PF00082	0.378
CLV_PCSK_KEX2_1	163	165	PF00082	0.419
CLV_PCSK_KEX2_1	167	169	PF00082	0.403
CLV_PCSK_KEX2_1	4	6	PF00082	0.334
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.378
CLV_PCSK_PC7_1	163	169	PF00082	0.423
CLV_PCSK_SKI1_1	106	110	PF00082	0.382
CLV_PCSK_SKI1_1	241	245	PF00082	0.497
DEG_SPOP_SBC_1	43	47	PF00917	0.363
DOC_CYCLIN_RxL_1	103	112	PF00134	0.178
DOC_CYCLIN_yClb1_LxF_4	190	195	PF00134	0.223
DOC_CYCLIN_yCln2_LP_2	282	288	PF00134	0.342
DOC_MAPK_gen_1	163	174	PF00069	0.210
DOC_MAPK_gen_1	261	270	PF00069	0.378
DOC_MAPK_gen_1	85	93	PF00069	0.234
DOC_MAPK_MEF2A_6	12	21	PF00069	0.431
DOC_MAPK_MEF2A_6	85	93	PF00069	0.208
DOC_PP1_RVXF_1	190	196	PF00149	0.210
DOC_PP1_RVXF_1	57	64	PF00149	0.300
DOC_PP2B_LxvP_1	282	285	PF13499	0.345
DOC_PP4_FxxP_1	122	125	PF00568	0.201
DOC_PP4_FxxP_1	234	237	PF00568	0.283
DOC_USP7_MATH_1	219	223	PF00917	0.343
LIG_14-3-3_CanoR_1	12	18	PF00244	0.410
LIG_14-3-3_CanoR_1	145	149	PF00244	0.160
LIG_14-3-3_CanoR_1	241	249	PF00244	0.286
LIG_14-3-3_CanoR_1	59	64	PF00244	0.268
LIG_Actin_WH2_2	46	61	PF00022	0.227
LIG_AP2alpha_2	100	102	PF02296	0.212
LIG_APCC_ABBA_1	172	177	PF00400	0.212
LIG_BIR_II_1	1	5	PF00653	0.622
LIG_BRCT_BRCA1_1	40	44	PF00533	0.363
LIG_deltaCOP1_diTrp_1	112	122	PF00928	0.201
LIG_EH_1	188	192	PF12763	0.306
LIG_EH_1	69	73	PF12763	0.244
LIG_EH1_1	17	25	PF00400	0.249
LIG_eIF4E_1	18	24	PF01652	0.375
LIG_FHA_1	14	20	PF00498	0.221
LIG_FHA_1	212	218	PF00498	0.343
LIG_FHA_1	34	40	PF00498	0.221
LIG_FHA_1	5	11	PF00498	0.498
LIG_FHA_2	43	49	PF00498	0.334
LIG_LIR_Apic_2	233	237	PF02991	0.274
LIG_LIR_Gen_1	100	110	PF02991	0.245
LIG_LIR_Gen_1	147	155	PF02991	0.314
LIG_LIR_Gen_1	16	25	PF02991	0.271
LIG_LIR_Nem_3	100	105	PF02991	0.243
LIG_LIR_Nem_3	147	151	PF02991	0.325
LIG_LIR_Nem_3	16	21	PF02991	0.273
LIG_LIR_Nem_3	229	234	PF02991	0.321
LIG_NRBOX	9	15	PF00104	0.414
LIG_Pex14_2	122	126	PF04695	0.230
LIG_Pex14_2	191	195	PF04695	0.201
LIG_SH2_STAT5	18	21	PF00017	0.275
LIG_SH2_STAT5	231	234	PF00017	0.250
LIG_SH3_2	187	192	PF14604	0.306
LIG_SH3_3	181	187	PF00018	0.263
LIG_SH3_3	294	300	PF00018	0.382
LIG_SUMO_SIM_par_1	207	212	PF11976	0.249
LIG_SUMO_SIM_par_1	283	289	PF11976	0.345
LIG_TRAF2_1	245	248	PF00917	0.289
LIG_WRC_WIRS_1	231	236	PF05994	0.365
MOD_CK1_1	147	153	PF00069	0.315
MOD_CK1_1	33	39	PF00069	0.206
MOD_CK2_1	209	215	PF00069	0.256
MOD_CK2_1	242	248	PF00069	0.294
MOD_CK2_1	42	48	PF00069	0.368
MOD_GlcNHglycan	211	214	PF01048	0.436
MOD_GlcNHglycan	221	224	PF01048	0.482
MOD_GlcNHglycan	244	247	PF01048	0.597
MOD_GlcNHglycan	282	285	PF01048	0.562
MOD_GlcNHglycan	96	99	PF01048	0.411
MOD_GSK3_1	147	154	PF00069	0.214
MOD_GSK3_1	280	287	PF00069	0.543
MOD_GSK3_1	38	45	PF00069	0.445
MOD_NEK2_1	13	18	PF00069	0.294
MOD_NEK2_1	19	24	PF00069	0.296
MOD_NEK2_1	209	214	PF00069	0.294
MOD_NEK2_2	101	106	PF00069	0.281
MOD_NEK2_2	71	76	PF00069	0.250
MOD_PIKK_1	247	253	PF00454	0.271
MOD_PIKK_1	30	36	PF00454	0.203
MOD_PKA_1	144	150	PF00069	0.271
MOD_PKA_1	4	10	PF00069	0.460
MOD_PKA_2	144	150	PF00069	0.160
MOD_PKA_2	219	225	PF00069	0.377
MOD_PKA_2	4	10	PF00069	0.460
MOD_Plk_4	111	117	PF00069	0.247
MOD_Plk_4	13	19	PF00069	0.295
MOD_Plk_4	202	208	PF00069	0.238
MOD_Plk_4	33	39	PF00069	0.236
MOD_Plk_4	59	65	PF00069	0.317
MOD_SUMO_rev_2	283	293	PF00179	0.490
TRG_DiLeu_BaLyEn_6	9	14	PF01217	0.490
TRG_ENDOCYTIC_2	18	21	PF00928	0.283
TRG_ENDOCYTIC_2	231	234	PF00928	0.344
TRG_ER_diArg_1	166	168	PF00400	0.199

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6B0	Leptomonas seymouri	79%	100%
A0A0N1I6V1	Leptomonas seymouri	24%	99%
A0A0S4JRP7	Bodo saltans	50%	100%
A0A1X0NUQ7	Trypanosomatidae	58%	100%
A0A3Q8IFY7	Leishmania donovani	94%	100%
A0A3S5IQW0	Trypanosoma rangeli	59%	100%
A0A3S7WNW6	Leishmania donovani	28%	85%
A4H420	Leishmania braziliensis	31%	86%
A4HKI5	Leishmania braziliensis	86%	100%
A4HSA2	Leishmania infantum	28%	85%
A4I4U1	Leishmania infantum	23%	94%
A4I816	Leishmania infantum	94%	100%
D0AA81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9AE89	Leishmania major	23%	100%
E9AK86	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	99%
E9B2X4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q42670	Cocos nucifera	27%	99%
V5ALB6	Trypanosoma cruzi	59%	100%