Phosphatidate cytidylyltransferase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

phosphatidate cytidylyltransferase-like protein

Species:

Leishmania major

UniProt:

Q4Q4Y8_LEIMA

TriTrypDb:

LmjF.32.2870 , LMJLV39_320036200 , LMJSD75_320036400

Length:

488

Annotations

LeishMANIAdb annotations

Related to bacterial cytidyltransferase enzymes. Relatively conserved architecture, with the expection of an insertion of 2 helices.. Might have been acquired by horizontal gene transfer in the ancestors of Kinetoplastids.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

Q4Q4Y8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4Y8

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	8
GO:0006644	phospholipid metabolic process	4	8
GO:0006650	glycerophospholipid metabolic process	5	8
GO:0006793	phosphorus metabolic process	3	8
GO:0006796	phosphate-containing compound metabolic process	4	8
GO:0008152	metabolic process	1	8
GO:0008610	lipid biosynthetic process	4	8
GO:0008654	phospholipid biosynthetic process	5	8
GO:0009058	biosynthetic process	2	8
GO:0009987	cellular process	1	8
GO:0016024	CDP-diacylglycerol biosynthetic process	6	8
GO:0019637	organophosphate metabolic process	3	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0044249	cellular biosynthetic process	3	8
GO:0044255	cellular lipid metabolic process	3	8
GO:0045017	glycerolipid biosynthetic process	4	8
GO:0046341	CDP-diacylglycerol metabolic process	6	8
GO:0046474	glycerophospholipid biosynthetic process	5	8
GO:0046486	glycerolipid metabolic process	4	8
GO:0071704	organic substance metabolic process	2	8
GO:0090407	organophosphate biosynthetic process	4	8
GO:1901576	organic substance biosynthetic process	3	8

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004605	phosphatidate cytidylyltransferase activity	6	9
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0070567	cytidylyltransferase activity	5	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	195	199	PF00656	0.571
CLV_C14_Caspase3-7	453	457	PF00656	0.482
CLV_NRD_NRD_1	335	337	PF00675	0.484
CLV_NRD_NRD_1	482	484	PF00675	0.390
CLV_PCSK_KEX2_1	186	188	PF00082	0.446
CLV_PCSK_KEX2_1	337	339	PF00082	0.474
CLV_PCSK_KEX2_1	433	435	PF00082	0.282
CLV_PCSK_KEX2_1	482	484	PF00082	0.390
CLV_PCSK_KEX2_1	70	72	PF00082	0.333
CLV_PCSK_PC1ET2_1	186	188	PF00082	0.387
CLV_PCSK_PC1ET2_1	337	339	PF00082	0.474
CLV_PCSK_PC1ET2_1	433	435	PF00082	0.282
CLV_PCSK_PC1ET2_1	70	72	PF00082	0.347
CLV_PCSK_SKI1_1	228	232	PF00082	0.445
CLV_PCSK_SKI1_1	29	33	PF00082	0.376
CLV_PCSK_SKI1_1	338	342	PF00082	0.470
CLV_PCSK_SKI1_1	348	352	PF00082	0.432
CLV_PCSK_SKI1_1	390	394	PF00082	0.475
CLV_PCSK_SKI1_1	483	487	PF00082	0.489
CLV_PCSK_SKI1_1	49	53	PF00082	0.150
CLV_PCSK_SKI1_1	71	75	PF00082	0.336
DEG_APCC_DBOX_1	19	27	PF00400	0.705
DEG_MDM2_SWIB_1	262	270	PF02201	0.335
DEG_Nend_UBRbox_2	1	3	PF02207	0.744
DEG_SCF_TRCP1_1	478	484	PF00400	0.579
DOC_CKS1_1	270	275	PF01111	0.480
DOC_CKS1_1	4	9	PF01111	0.746
DOC_CYCLIN_RxL_1	345	353	PF00134	0.668
DOC_CYCLIN_yCln2_LP_2	460	466	PF00134	0.562
DOC_MAPK_DCC_7	88	98	PF00069	0.615
DOC_MAPK_gen_1	70	77	PF00069	0.539
DOC_MAPK_MEF2A_6	454	462	PF00069	0.496
DOC_MAPK_MEF2A_6	5	14	PF00069	0.600
DOC_MAPK_MEF2A_6	70	79	PF00069	0.542
DOC_PP1_RVXF_1	135	142	PF00149	0.447
DOC_PP4_FxxP_1	393	396	PF00568	0.302
DOC_PP4_FxxP_1	443	446	PF00568	0.553
DOC_USP7_MATH_1	363	367	PF00917	0.487
DOC_USP7_UBL2_3	337	341	PF12436	0.636
DOC_USP7_UBL2_3	70	74	PF12436	0.545
DOC_WW_Pin1_4	107	112	PF00397	0.775
DOC_WW_Pin1_4	269	274	PF00397	0.466
DOC_WW_Pin1_4	3	8	PF00397	0.789
DOC_WW_Pin1_4	350	355	PF00397	0.602
DOC_WW_Pin1_4	359	364	PF00397	0.445
DOC_WW_Pin1_4	88	93	PF00397	0.741
LIG_14-3-3_CanoR_1	29	34	PF00244	0.396
LIG_14-3-3_CanoR_1	470	476	PF00244	0.594
LIG_14-3-3_CanoR_1	482	486	PF00244	0.528
LIG_14-3-3_CanoR_1	49	54	PF00244	0.150
LIG_Actin_WH2_2	420	435	PF00022	0.344
LIG_BRCT_BRCA1_1	270	274	PF00533	0.246
LIG_Clathr_ClatBox_1	156	160	PF01394	0.480
LIG_Clathr_ClatBox_1	78	82	PF01394	0.649
LIG_FHA_1	156	162	PF00498	0.340
LIG_FHA_1	28	34	PF00498	0.380
LIG_FHA_1	95	101	PF00498	0.714
LIG_FHA_2	210	216	PF00498	0.735
LIG_FHA_2	320	326	PF00498	0.701
LIG_GSK3_LRP6_1	107	112	PF00069	0.709
LIG_LIR_Apic_2	121	126	PF02991	0.621
LIG_LIR_Apic_2	2	7	PF02991	0.629
LIG_LIR_Apic_2	440	446	PF02991	0.521
LIG_LIR_Gen_1	131	139	PF02991	0.511
LIG_LIR_Gen_1	160	171	PF02991	0.375
LIG_LIR_Gen_1	235	245	PF02991	0.605
LIG_LIR_Gen_1	251	262	PF02991	0.416
LIG_LIR_Gen_1	402	413	PF02991	0.347
LIG_LIR_Gen_1	55	64	PF02991	0.438
LIG_LIR_Nem_3	131	136	PF02991	0.593
LIG_LIR_Nem_3	160	166	PF02991	0.354
LIG_LIR_Nem_3	235	239	PF02991	0.595
LIG_LIR_Nem_3	251	257	PF02991	0.531
LIG_LIR_Nem_3	260	265	PF02991	0.323
LIG_LIR_Nem_3	271	277	PF02991	0.249
LIG_LIR_Nem_3	313	318	PF02991	0.638
LIG_LIR_Nem_3	380	384	PF02991	0.430
LIG_LIR_Nem_3	402	408	PF02991	0.319
LIG_LIR_Nem_3	55	60	PF02991	0.365
LIG_LIR_Nem_3	65	69	PF02991	0.315
LIG_NRBOX	409	415	PF00104	0.430
LIG_NRBOX	427	433	PF00104	0.225
LIG_PDZ_Class_2	483	488	PF00595	0.758
LIG_Pex14_2	262	266	PF04695	0.335
LIG_Pex14_2	56	60	PF04695	0.359
LIG_SH2_CRK	133	137	PF00017	0.498
LIG_SH2_CRK	389	393	PF00017	0.275
LIG_SH2_CRK	4	8	PF00017	0.629
LIG_SH2_STAT5	162	165	PF00017	0.332
LIG_SH2_STAT5	236	239	PF00017	0.661
LIG_SH3_1	105	111	PF00018	0.735
LIG_SH3_1	123	129	PF00018	0.644
LIG_SH3_1	211	217	PF00018	0.622
LIG_SH3_1	4	10	PF00018	0.628
LIG_SH3_3	100	106	PF00018	0.600
LIG_SH3_3	114	120	PF00018	0.703
LIG_SH3_3	123	129	PF00018	0.618
LIG_SH3_3	211	217	PF00018	0.682
LIG_SH3_3	279	285	PF00018	0.397
LIG_SH3_3	397	403	PF00018	0.362
LIG_SH3_3	4	10	PF00018	0.701
LIG_SH3_3	86	92	PF00018	0.798
LIG_SUMO_SIM_anti_2	307	313	PF11976	0.557
LIG_SUMO_SIM_par_1	155	160	PF11976	0.479
LIG_SUMO_SIM_par_1	167	172	PF11976	0.389
LIG_TRAF2_1	322	325	PF00917	0.660
LIG_UBA3_1	427	433	PF00899	0.335
LIG_WRC_WIRS_1	53	58	PF05994	0.428
MOD_CDC14_SPxK_1	362	365	PF00782	0.482
MOD_CDK_SPxK_1	359	365	PF00069	0.482
MOD_CK1_1	193	199	PF00069	0.762
MOD_CK1_1	44	50	PF00069	0.400
MOD_CK1_1	474	480	PF00069	0.669
MOD_CK2_1	319	325	PF00069	0.631
MOD_DYRK1A_RPxSP_1	359	363	PF00069	0.553
MOD_GlcNHglycan	149	152	PF01048	0.548
MOD_GlcNHglycan	342	346	PF01048	0.354
MOD_GlcNHglycan	43	46	PF01048	0.519
MOD_GlcNHglycan	478	481	PF01048	0.521
MOD_GlcNHglycan	65	69	PF01048	0.340
MOD_GSK3_1	186	193	PF00069	0.676
MOD_GSK3_1	359	366	PF00069	0.482
MOD_GSK3_1	52	59	PF00069	0.381
MOD_GSK3_1	77	84	PF00069	0.664
MOD_N-GLC_1	363	368	PF02516	0.334
MOD_NEK2_1	287	292	PF00069	0.426
MOD_NEK2_1	41	46	PF00069	0.594
MOD_NEK2_1	413	418	PF00069	0.354
MOD_NEK2_1	427	432	PF00069	0.294
MOD_NEK2_1	56	61	PF00069	0.247
MOD_NEK2_1	81	86	PF00069	0.716
MOD_PIKK_1	186	192	PF00454	0.712
MOD_PIKK_1	193	199	PF00454	0.717
MOD_PKA_1	186	192	PF00069	0.598
MOD_PKA_2	186	192	PF00069	0.614
MOD_PKA_2	481	487	PF00069	0.723
MOD_Plk_1	219	225	PF00069	0.740
MOD_Plk_1	363	369	PF00069	0.534
MOD_Plk_1	81	87	PF00069	0.751
MOD_Plk_4	287	293	PF00069	0.358
MOD_Plk_4	413	419	PF00069	0.398
MOD_Plk_4	427	433	PF00069	0.283
MOD_Plk_4	49	55	PF00069	0.419
MOD_Plk_4	56	62	PF00069	0.452
MOD_Plk_4	94	100	PF00069	0.722
MOD_ProDKin_1	107	113	PF00069	0.775
MOD_ProDKin_1	269	275	PF00069	0.466
MOD_ProDKin_1	3	9	PF00069	0.788
MOD_ProDKin_1	350	356	PF00069	0.602
MOD_ProDKin_1	359	365	PF00069	0.445
MOD_ProDKin_1	88	94	PF00069	0.737
MOD_SUMO_for_1	179	182	PF00179	0.656
MOD_SUMO_for_1	224	227	PF00179	0.739
TRG_DiLeu_BaEn_1	152	157	PF01217	0.335
TRG_DiLeu_BaEn_1	342	347	PF01217	0.614
TRG_ENDOCYTIC_2	133	136	PF00928	0.539
TRG_ENDOCYTIC_2	162	165	PF00928	0.376
TRG_ENDOCYTIC_2	236	239	PF00928	0.643
TRG_ENDOCYTIC_2	315	318	PF00928	0.641
TRG_ENDOCYTIC_2	389	392	PF00928	0.339
TRG_ER_diArg_1	18	21	PF00400	0.723
TRG_ER_diArg_1	335	338	PF00400	0.674
TRG_NLS_MonoExtN_4	335	340	PF00514	0.655
TRG_Pf-PMV_PEXEL_1	338	342	PF00026	0.465

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEC8	Leptomonas seymouri	63%	100%
A0A0S4IRI0	Bodo saltans	31%	100%
A0A1X0NT53	Trypanosomatidae	31%	100%
A0A3Q8IDC1	Leishmania donovani	89%	99%
A0A422NZP0	Trypanosoma rangeli	34%	100%
A4HKS8	Leishmania braziliensis	67%	100%
E9AHM2	Leishmania infantum	89%	99%
E9B366	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	98%
V5AXY5	Trypanosoma cruzi	33%	100%