TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania major

UniProt:

Q4Q4T4_LEIMA

TriTrypDb:

LmjF.32.3380 , LMJLV39_320042000 , LMJSD75_320042000

Length:

709

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 9
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q4T4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4T4

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0009982	pseudouridine synthase activity	4	11
GO:0016853	isomerase activity	2	11
GO:0016866	intramolecular transferase activity	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1
GO:0016829	lyase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	178	182	PF00656	0.554
CLV_NRD_NRD_1	124	126	PF00675	0.680
CLV_NRD_NRD_1	149	151	PF00675	0.470
CLV_NRD_NRD_1	157	159	PF00675	0.479
CLV_NRD_NRD_1	196	198	PF00675	0.718
CLV_NRD_NRD_1	206	208	PF00675	0.665
CLV_NRD_NRD_1	233	235	PF00675	0.267
CLV_NRD_NRD_1	352	354	PF00675	0.270
CLV_NRD_NRD_1	628	630	PF00675	0.440
CLV_NRD_NRD_1	663	665	PF00675	0.558
CLV_NRD_NRD_1	85	87	PF00675	0.375
CLV_PCSK_FUR_1	194	198	PF00082	0.744
CLV_PCSK_KEX2_1	124	126	PF00082	0.680
CLV_PCSK_KEX2_1	149	151	PF00082	0.471
CLV_PCSK_KEX2_1	157	159	PF00082	0.480
CLV_PCSK_KEX2_1	196	198	PF00082	0.680
CLV_PCSK_KEX2_1	206	208	PF00082	0.665
CLV_PCSK_KEX2_1	235	237	PF00082	0.249
CLV_PCSK_KEX2_1	266	268	PF00082	0.248
CLV_PCSK_KEX2_1	352	354	PF00082	0.311
CLV_PCSK_KEX2_1	628	630	PF00082	0.555
CLV_PCSK_KEX2_1	663	665	PF00082	0.668
CLV_PCSK_PC1ET2_1	235	237	PF00082	0.249
CLV_PCSK_PC1ET2_1	266	268	PF00082	0.248
CLV_PCSK_SKI1_1	244	248	PF00082	0.339
CLV_PCSK_SKI1_1	290	294	PF00082	0.313
CLV_PCSK_SKI1_1	374	378	PF00082	0.313
CLV_PCSK_SKI1_1	490	494	PF00082	0.366
CLV_PCSK_SKI1_1	585	589	PF00082	0.652
CLV_PCSK_SKI1_1	700	704	PF00082	0.324
CLV_PCSK_SKI1_1	86	90	PF00082	0.491
CLV_Separin_Metazoa	154	158	PF03568	0.471
DEG_APCC_DBOX_1	81	89	PF00400	0.481
DEG_MDM2_SWIB_1	414	422	PF02201	0.382
DEG_SPOP_SBC_1	550	554	PF00917	0.554
DEG_SPOP_SBC_1	678	682	PF00917	0.459
DOC_ANK_TNKS_1	124	131	PF00023	0.492
DOC_ANK_TNKS_1	176	183	PF00023	0.618
DOC_CYCLIN_RxL_1	286	294	PF00134	0.310
DOC_CYCLIN_yCln2_LP_2	593	599	PF00134	0.393
DOC_MAPK_gen_1	194	203	PF00069	0.489
DOC_MAPK_gen_1	264	271	PF00069	0.313
DOC_MAPK_gen_1	628	635	PF00069	0.351
DOC_PP1_RVXF_1	340	347	PF00149	0.382
DOC_PP1_RVXF_1	613	620	PF00149	0.443
DOC_PP4_FxxP_1	687	690	PF00568	0.297
DOC_USP7_MATH_1	136	140	PF00917	0.677
DOC_USP7_MATH_1	141	145	PF00917	0.658
DOC_USP7_MATH_1	180	184	PF00917	0.582
DOC_USP7_MATH_1	192	196	PF00917	0.530
DOC_USP7_MATH_1	307	311	PF00917	0.407
DOC_USP7_MATH_1	320	324	PF00917	0.431
DOC_USP7_MATH_1	421	425	PF00917	0.268
DOC_USP7_MATH_1	471	475	PF00917	0.412
DOC_USP7_MATH_1	507	511	PF00917	0.301
DOC_USP7_MATH_1	545	549	PF00917	0.704
DOC_USP7_MATH_1	550	554	PF00917	0.659
DOC_WW_Pin1_4	217	222	PF00397	0.576
DOC_WW_Pin1_4	354	359	PF00397	0.365
LIG_14-3-3_CanoR_1	194	203	PF00244	0.725
LIG_14-3-3_CanoR_1	267	272	PF00244	0.298
LIG_14-3-3_CanoR_1	273	277	PF00244	0.243
LIG_14-3-3_CanoR_1	319	329	PF00244	0.424
LIG_14-3-3_CanoR_1	397	405	PF00244	0.298
LIG_14-3-3_CanoR_1	443	452	PF00244	0.279
LIG_14-3-3_CanoR_1	460	464	PF00244	0.248
LIG_14-3-3_CanoR_1	527	537	PF00244	0.637
LIG_14-3-3_CanoR_1	618	625	PF00244	0.498
LIG_14-3-3_CanoR_1	63	67	PF00244	0.514
LIG_BIR_III_2	17	21	PF00653	0.507
LIG_BIR_III_2	218	222	PF00653	0.563
LIG_BRCT_BRCA1_1	112	116	PF00533	0.502
LIG_BRCT_BRCA1_1	509	513	PF00533	0.249
LIG_deltaCOP1_diTrp_1	616	625	PF00928	0.402
LIG_FHA_1	454	460	PF00498	0.382
LIG_FHA_1	491	497	PF00498	0.442
LIG_FHA_1	680	686	PF00498	0.411
LIG_FHA_1	694	700	PF00498	0.240
LIG_FHA_2	112	118	PF00498	0.568
LIG_FHA_2	176	182	PF00498	0.625
LIG_FHA_2	206	212	PF00498	0.734
LIG_FHA_2	279	285	PF00498	0.289
LIG_FHA_2	358	364	PF00498	0.372
LIG_LIR_Apic_2	686	690	PF02991	0.302
LIG_LIR_Gen_1	113	123	PF02991	0.559
LIG_LIR_Gen_1	313	322	PF02991	0.304
LIG_LIR_Gen_1	431	440	PF02991	0.358
LIG_LIR_Gen_1	481	492	PF02991	0.292
LIG_LIR_Gen_1	692	702	PF02991	0.286
LIG_LIR_Nem_3	113	119	PF02991	0.528
LIG_LIR_Nem_3	249	253	PF02991	0.327
LIG_LIR_Nem_3	313	317	PF02991	0.293
LIG_LIR_Nem_3	333	339	PF02991	0.223
LIG_LIR_Nem_3	386	392	PF02991	0.250
LIG_LIR_Nem_3	431	436	PF02991	0.358
LIG_LIR_Nem_3	462	466	PF02991	0.342
LIG_LIR_Nem_3	481	487	PF02991	0.295
LIG_LIR_Nem_3	510	515	PF02991	0.327
LIG_LIR_Nem_3	638	642	PF02991	0.467
LIG_LIR_Nem_3	692	697	PF02991	0.291
LIG_LIR_Nem_3	94	100	PF02991	0.537
LIG_LYPXL_S_1	159	163	PF13949	0.519
LIG_LYPXL_yS_3	160	163	PF13949	0.374
LIG_MLH1_MIPbox_1	509	513	PF16413	0.382
LIG_NRBOX	84	90	PF00104	0.419
LIG_Pex14_2	389	393	PF04695	0.249
LIG_Pex14_2	414	418	PF04695	0.375
LIG_SH2_CRK	314	318	PF00017	0.382
LIG_SH2_CRK	694	698	PF00017	0.274
LIG_SH2_NCK_1	694	698	PF00017	0.289
LIG_SH2_PTP2	228	231	PF00017	0.313
LIG_SH2_SRC	228	231	PF00017	0.313
LIG_SH2_STAP1	314	318	PF00017	0.329
LIG_SH2_STAP1	679	683	PF00017	0.419
LIG_SH2_STAP1	694	698	PF00017	0.270
LIG_SH2_STAT3	392	395	PF00017	0.249
LIG_SH2_STAT5	228	231	PF00017	0.313
LIG_SH2_STAT5	250	253	PF00017	0.327
LIG_SH2_STAT5	392	395	PF00017	0.249
LIG_SH2_STAT5	466	469	PF00017	0.362
LIG_SH2_STAT5	499	502	PF00017	0.382
LIG_SH2_STAT5	519	522	PF00017	0.128
LIG_SH3_1	125	131	PF00018	0.526
LIG_SH3_3	125	131	PF00018	0.526
LIG_SH3_3	16	22	PF00018	0.698
LIG_SH3_3	559	565	PF00018	0.469
LIG_SH3_3	630	636	PF00018	0.348
LIG_SH3_3	640	646	PF00018	0.321
LIG_TRAF2_1	71	74	PF00917	0.453
LIG_TRFH_1	687	691	PF08558	0.347
LIG_WW_2	19	22	PF00397	0.518
MOD_CK1_1	115	121	PF00069	0.681
MOD_CK1_1	183	189	PF00069	0.690
MOD_CK1_1	195	201	PF00069	0.576
MOD_CK1_1	220	226	PF00069	0.580
MOD_CK1_1	310	316	PF00069	0.274
MOD_CK1_1	321	327	PF00069	0.326
MOD_CK1_1	357	363	PF00069	0.368
MOD_CK1_1	36	42	PF00069	0.684
MOD_CK1_1	446	452	PF00069	0.291
MOD_CK1_1	553	559	PF00069	0.714
MOD_CK1_1	91	97	PF00069	0.502
MOD_CK2_1	115	121	PF00069	0.560
MOD_CK2_1	205	211	PF00069	0.620
MOD_CK2_1	278	284	PF00069	0.289
MOD_CK2_1	357	363	PF00069	0.349
MOD_CK2_1	554	560	PF00069	0.575
MOD_CMANNOS	602	605	PF00535	0.465
MOD_GlcNHglycan	103	106	PF01048	0.523
MOD_GlcNHglycan	197	200	PF01048	0.679
MOD_GlcNHglycan	222	225	PF01048	0.622
MOD_GlcNHglycan	29	32	PF01048	0.703
MOD_GlcNHglycan	320	323	PF01048	0.299
MOD_GlcNHglycan	332	335	PF01048	0.320
MOD_GlcNHglycan	473	476	PF01048	0.396
MOD_GlcNHglycan	538	542	PF01048	0.707
MOD_GlcNHglycan	547	550	PF01048	0.656
MOD_GlcNHglycan	553	556	PF01048	0.577
MOD_GlcNHglycan	572	575	PF01048	0.533
MOD_GlcNHglycan	578	581	PF01048	0.396
MOD_GlcNHglycan	597	600	PF01048	0.541
MOD_GlcNHglycan	620	623	PF01048	0.503
MOD_GlcNHglycan	666	669	PF01048	0.560
MOD_GlcNHglycan	672	676	PF01048	0.727
MOD_GlcNHglycan	77	80	PF01048	0.385
MOD_GSK3_1	111	118	PF00069	0.599
MOD_GSK3_1	478	485	PF00069	0.382
MOD_GSK3_1	515	522	PF00069	0.366
MOD_GSK3_1	545	552	PF00069	0.698
MOD_GSK3_1	591	598	PF00069	0.448
MOD_GSK3_1	679	686	PF00069	0.368
MOD_GSK3_1	689	696	PF00069	0.309
MOD_N-GLC_1	183	188	PF02516	0.578
MOD_N-GLC_1	33	38	PF02516	0.581
MOD_N-GLC_1	478	483	PF02516	0.362
MOD_N-GLC_1	647	652	PF02516	0.287
MOD_NEK2_1	111	116	PF00069	0.527
MOD_NEK2_1	318	323	PF00069	0.321
MOD_NEK2_1	444	449	PF00069	0.379
MOD_NEK2_1	459	464	PF00069	0.276
MOD_NEK2_1	49	54	PF00069	0.383
MOD_NEK2_1	515	520	PF00069	0.283
MOD_NEK2_1	588	593	PF00069	0.397
MOD_NEK2_1	617	622	PF00069	0.400
MOD_NEK2_1	683	688	PF00069	0.320
MOD_NEK2_2	170	175	PF00069	0.458
MOD_NEK2_2	421	426	PF00069	0.382
MOD_NEK2_2	507	512	PF00069	0.382
MOD_NEK2_2	693	698	PF00069	0.391
MOD_PIKK_1	307	313	PF00454	0.182
MOD_PK_1	254	260	PF00069	0.271
MOD_PK_1	267	273	PF00069	0.235
MOD_PKA_2	195	201	PF00069	0.723
MOD_PKA_2	205	211	PF00069	0.688
MOD_PKA_2	272	278	PF00069	0.249
MOD_PKA_2	318	324	PF00069	0.432
MOD_PKA_2	396	402	PF00069	0.267
MOD_PKA_2	459	465	PF00069	0.336
MOD_PKA_2	617	623	PF00069	0.472
MOD_PKA_2	62	68	PF00069	0.546
MOD_PKA_2	644	650	PF00069	0.409
MOD_PKA_2	662	668	PF00069	0.497
MOD_Plk_1	183	189	PF00069	0.579
MOD_Plk_1	254	260	PF00069	0.248
MOD_Plk_1	33	39	PF00069	0.578
MOD_Plk_1	647	653	PF00069	0.428
MOD_Plk_4	33	39	PF00069	0.647
MOD_Plk_4	507	513	PF00069	0.291
MOD_Plk_4	515	521	PF00069	0.306
MOD_Plk_4	693	699	PF00069	0.282
MOD_ProDKin_1	217	223	PF00069	0.571
MOD_ProDKin_1	354	360	PF00069	0.365
TRG_DiLeu_BaEn_1	242	247	PF01217	0.267
TRG_DiLeu_BaEn_1	84	89	PF01217	0.412
TRG_DiLeu_BaEn_3	371	377	PF01217	0.248
TRG_ENDOCYTIC_2	160	163	PF00928	0.403
TRG_ENDOCYTIC_2	228	231	PF00928	0.313
TRG_ENDOCYTIC_2	250	253	PF00928	0.327
TRG_ENDOCYTIC_2	314	317	PF00928	0.252
TRG_ENDOCYTIC_2	433	436	PF00928	0.283
TRG_ENDOCYTIC_2	694	697	PF00928	0.278
TRG_ER_diArg_1	100	103	PF00400	0.582
TRG_ER_diArg_1	123	125	PF00400	0.652
TRG_ER_diArg_1	148	150	PF00400	0.427
TRG_ER_diArg_1	156	158	PF00400	0.391
TRG_ER_diArg_1	176	179	PF00400	0.362
TRG_ER_diArg_1	194	197	PF00400	0.684
TRG_ER_diArg_1	206	209	PF00400	0.606
TRG_ER_diArg_1	233	236	PF00400	0.267
TRG_ER_diArg_1	339	342	PF00400	0.331
TRG_ER_diArg_1	352	354	PF00400	0.340
TRG_ER_diArg_1	628	630	PF00400	0.551
TRG_NES_CRM1_1	242	255	PF08389	0.292
TRG_NLS_MonoExtC_3	233	239	PF00514	0.249
TRG_Pf-PMV_PEXEL_1	244	249	PF00026	0.267
TRG_Pf-PMV_PEXEL_1	75	80	PF00026	0.575
TRG_Pf-PMV_PEXEL_1	86	90	PF00026	0.539

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4F4	Leptomonas seymouri	50%	74%
A0A1X0NRV3	Trypanosomatidae	33%	100%
A0A3Q8IJ87	Leishmania donovani	23%	100%
A0A3R7LD82	Trypanosoma rangeli	33%	100%
A0A3S7X658	Leishmania donovani	92%	99%
A4HKY3	Leishmania braziliensis	73%	75%
A4I8G2	Leishmania infantum	92%	76%
A4IDY9	Leishmania infantum	23%	100%
D0AAQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9B3C1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	99%
O74343	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
Q08647	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	100%
Q1L8I0	Danio rerio	23%	100%
Q4Q0I6	Leishmania major	22%	100%
V5BLA6	Trypanosoma cruzi	33%	100%