tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania major

UniProt:

Q4Q4P7_LEIMA

TriTrypDb:

LmjF.32.3750 , LMJLV39_320045400 , LMJSD75_320045500

Length:

273

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 18
NetGPI	no	yes: 0, no: 18

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

Q4Q4P7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4P7

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	3
GO:0006139	nucleobase-containing compound metabolic process	3	19
GO:0006396	RNA processing	6	19
GO:0006399	tRNA metabolic process	7	19
GO:0006400	tRNA modification	6	3
GO:0006725	cellular aromatic compound metabolic process	3	19
GO:0006807	nitrogen compound metabolic process	2	19
GO:0008033	tRNA processing	8	19
GO:0008152	metabolic process	1	19
GO:0009451	RNA modification	5	3
GO:0009987	cellular process	1	19
GO:0016070	RNA metabolic process	5	19
GO:0030488	tRNA methylation	5	3
GO:0032259	methylation	2	19
GO:0034470	ncRNA processing	7	19
GO:0034641	cellular nitrogen compound metabolic process	3	19
GO:0034660	ncRNA metabolic process	6	19
GO:0043170	macromolecule metabolic process	3	19
GO:0043412	macromolecule modification	4	3
GO:0043414	macromolecule methylation	3	3
GO:0044237	cellular metabolic process	2	19
GO:0044238	primary metabolic process	2	19
GO:0044260	obsolete cellular macromolecule metabolic process	3	3
GO:0046483	heterocycle metabolic process	3	19
GO:0071704	organic substance metabolic process	2	19
GO:0090304	nucleic acid metabolic process	4	19
GO:1901360	organic cyclic compound metabolic process	3	19

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	19
GO:0008168	methyltransferase activity	4	19
GO:0008173	RNA methyltransferase activity	4	3
GO:0008175	tRNA methyltransferase activity	5	3
GO:0016740	transferase activity	2	19
GO:0016741	transferase activity, transferring one-carbon groups	3	19
GO:0140098	catalytic activity, acting on RNA	3	3
GO:0140101	catalytic activity, acting on a tRNA	4	3
GO:0140640	catalytic activity, acting on a nucleic acid	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	188	190	PF00675	0.238
CLV_NRD_NRD_1	244	246	PF00675	0.235
CLV_NRD_NRD_1	86	88	PF00675	0.259
CLV_PCSK_FUR_1	86	90	PF00082	0.360
CLV_PCSK_KEX2_1	244	246	PF00082	0.238
CLV_PCSK_KEX2_1	86	88	PF00082	0.270
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.265
CLV_PCSK_SKI1_1	189	193	PF00082	0.228
CLV_PCSK_SKI1_1	49	53	PF00082	0.298
DEG_Nend_UBRbox_1	1	4	PF02207	0.217
DEG_SPOP_SBC_1	41	45	PF00917	0.287
DEG_SPOP_SBC_1	73	77	PF00917	0.203
DOC_MAPK_gen_1	244	252	PF00069	0.371
DOC_MAPK_MEF2A_6	145	153	PF00069	0.304
DOC_USP7_MATH_1	129	133	PF00917	0.336
DOC_USP7_MATH_1	69	73	PF00917	0.392
DOC_USP7_MATH_1	80	84	PF00917	0.423
DOC_USP7_UBL2_3	186	190	PF12436	0.343
DOC_WW_Pin1_4	194	199	PF00397	0.316
DOC_WW_Pin1_4	211	216	PF00397	0.212
DOC_WW_Pin1_4	62	67	PF00397	0.421
DOC_WW_Pin1_4	74	79	PF00397	0.390
LIG_14-3-3_CanoR_1	135	144	PF00244	0.419
LIG_14-3-3_CanoR_1	189	198	PF00244	0.313
LIG_deltaCOP1_diTrp_1	121	126	PF00928	0.389
LIG_deltaCOP1_diTrp_1	209	218	PF00928	0.340
LIG_deltaCOP1_diTrp_1	221	226	PF00928	0.344
LIG_FHA_1	136	142	PF00498	0.310
LIG_FHA_1	190	196	PF00498	0.266
LIG_FHA_1	208	214	PF00498	0.129
LIG_FHA_1	226	232	PF00498	0.241
LIG_FHA_1	36	42	PF00498	0.336
LIG_FHA_2	109	115	PF00498	0.335
LIG_FHA_2	241	247	PF00498	0.440
LIG_LIR_Gen_1	220	231	PF02991	0.389
LIG_LIR_Nem_3	220	226	PF02991	0.258
LIG_LIR_Nem_3	36	40	PF02991	0.287
LIG_MYND_3	117	121	PF01753	0.381
LIG_PTB_Apo_2	31	38	PF02174	0.259
LIG_SH2_GRB2like	32	35	PF00017	0.389
LIG_SH2_SRC	32	35	PF00017	0.416
LIG_SH2_STAT5	32	35	PF00017	0.279
LIG_SH2_STAT5	40	43	PF00017	0.254
LIG_SH3_3	198	204	PF00018	0.267
MOD_CK1_1	100	106	PF00069	0.160
MOD_CK1_1	182	188	PF00069	0.243
MOD_CK1_1	59	65	PF00069	0.377
MOD_CK1_1	72	78	PF00069	0.303
MOD_CK2_1	108	114	PF00069	0.297
MOD_CK2_1	240	246	PF00069	0.301
MOD_CK2_1	79	85	PF00069	0.464
MOD_GlcNHglycan	170	173	PF01048	0.287
MOD_GlcNHglycan	175	178	PF01048	0.297
MOD_GlcNHglycan	254	257	PF01048	0.525
MOD_GlcNHglycan	268	271	PF01048	0.354
MOD_GlcNHglycan	71	74	PF01048	0.235
MOD_GSK3_1	108	115	PF00069	0.268
MOD_GSK3_1	173	180	PF00069	0.268
MOD_GSK3_1	189	196	PF00069	0.259
MOD_GSK3_1	207	214	PF00069	0.125
MOD_GSK3_1	35	42	PF00069	0.302
MOD_GSK3_1	4	11	PF00069	0.397
MOD_GSK3_1	55	62	PF00069	0.190
MOD_GSK3_1	69	76	PF00069	0.358
MOD_GSK3_1	93	100	PF00069	0.297
MOD_N-GLC_1	240	245	PF02516	0.217
MOD_N-GLC_1	33	38	PF02516	0.342
MOD_NEK2_1	252	257	PF00069	0.571
MOD_NEK2_2	93	98	PF00069	0.303
MOD_PIKK_1	35	41	PF00454	0.246
MOD_PK_1	106	112	PF00069	0.160
MOD_PKA_1	189	195	PF00069	0.238
MOD_PKA_1	88	94	PF00069	0.303
MOD_PKA_2	112	118	PF00069	0.429
MOD_PKA_2	182	188	PF00069	0.276
MOD_PKA_2	88	94	PF00069	0.303
MOD_Plk_1	106	112	PF00069	0.389
MOD_Plk_1	14	20	PF00069	0.259
MOD_Plk_4	112	118	PF00069	0.125
MOD_Plk_4	233	239	PF00069	0.309
MOD_Plk_4	93	99	PF00069	0.297
MOD_ProDKin_1	194	200	PF00069	0.316
MOD_ProDKin_1	211	217	PF00069	0.212
MOD_ProDKin_1	62	68	PF00069	0.421
MOD_ProDKin_1	74	80	PF00069	0.390
MOD_SUMO_for_1	144	147	PF00179	0.265
TRG_ER_diArg_1	86	89	PF00400	0.282
TRG_NLS_MonoExtN_4	86	91	PF00514	0.393

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB24	Leptomonas seymouri	69%	96%
A0A0S4JMK0	Bodo saltans	26%	84%
A0A1X0NNC3	Trypanosomatidae	28%	79%
A0A1X0NRX8	Trypanosomatidae	54%	90%
A0A3Q8IBW6	Leishmania donovani	29%	78%
A0A3R7NUS4	Trypanosoma rangeli	54%	99%
A0A3S5IRY7	Trypanosoma rangeli	26%	84%
A0A3S7X653	Leishmania donovani	90%	100%
A4HL12	Leishmania braziliensis	80%	98%
A4HTY7	Leishmania infantum	28%	78%
A4I8J3	Leishmania infantum	90%	100%
D0A9E3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	77%
D0AAM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	92%
E9AMR7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	79%
E9B3F3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QHV8	Leishmania major	28%	78%
Q9UTA5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
V5DMF2	Trypanosoma cruzi	53%	98%