Cysteine protease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Cysteine protease

Gene product:

AUT2/APG4/ATG4 cysteine peptidase, putative

Species:

Leishmania major

UniProt:

Q4Q4N3_LEIMA

TriTrypDb:

LmjF.32.3890 , LMJLV39_320046800 , LMJSD75_320047000

Length:

388

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

Q4Q4N3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4N3

Function

Biological processes
Term	Name	Level	Count
GO:0000045	autophagosome assembly	6	11
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006810	transport	3	11
GO:0006996	organelle organization	4	11
GO:0007033	vacuole organization	5	11
GO:0008104	protein localization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016043	cellular component organization	3	11
GO:0019538	protein metabolic process	3	11
GO:0022607	cellular component assembly	4	11
GO:0033036	macromolecule localization	2	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0045184	establishment of protein localization	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051697	protein delipidation	5	11
GO:0070727	cellular macromolecule localization	3	11
GO:0070925	organelle assembly	5	11
GO:0071702	organic substance transport	4	11
GO:0071704	organic substance metabolic process	2	11
GO:0071705	nitrogen compound transport	4	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1905037	autophagosome organization	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	291	293	PF00675	0.233
CLV_NRD_NRD_1	332	334	PF00675	0.333
CLV_NRD_NRD_1	34	36	PF00675	0.366
CLV_PCSK_FUR_1	289	293	PF00082	0.142
CLV_PCSK_KEX2_1	291	293	PF00082	0.208
CLV_PCSK_KEX2_1	332	334	PF00082	0.333
CLV_PCSK_SKI1_1	258	262	PF00082	0.214
CLV_PCSK_SKI1_1	35	39	PF00082	0.549
CLV_PCSK_SKI1_1	61	65	PF00082	0.255
DEG_ODPH_VHL_1	218	230	PF01847	0.377
DEG_SPOP_SBC_1	271	275	PF00917	0.384
DOC_CKS1_1	30	35	PF01111	0.362
DOC_CKS1_1	303	308	PF01111	0.400
DOC_CYCLIN_RxL_1	61	71	PF00134	0.246
DOC_CYCLIN_yCln2_LP_2	210	216	PF00134	0.369
DOC_MAPK_gen_1	126	134	PF00069	0.499
DOC_MAPK_gen_1	289	299	PF00069	0.502
DOC_MAPK_gen_1	35	45	PF00069	0.426
DOC_MAPK_MEF2A_6	292	301	PF00069	0.506
DOC_MAPK_MEF2A_6	38	46	PF00069	0.285
DOC_PP1_RVXF_1	256	263	PF00149	0.452
DOC_PP1_RVXF_1	64	71	PF00149	0.470
DOC_PP2B_LxvP_1	210	213	PF13499	0.369
DOC_PP4_FxxP_1	262	265	PF00568	0.389
DOC_PP4_FxxP_1	30	33	PF00568	0.363
DOC_USP7_MATH_1	271	275	PF00917	0.405
DOC_USP7_MATH_1	363	367	PF00917	0.483
DOC_USP7_MATH_1	377	381	PF00917	0.480
DOC_USP7_MATH_1	78	82	PF00917	0.454
DOC_USP7_UBL2_3	150	154	PF12436	0.377
DOC_USP7_UBL2_3	6	10	PF12436	0.464
DOC_USP7_UBL2_3	61	65	PF12436	0.235
DOC_WW_Pin1_4	157	162	PF00397	0.380
DOC_WW_Pin1_4	29	34	PF00397	0.480
DOC_WW_Pin1_4	302	307	PF00397	0.370
DOC_WW_Pin1_4	38	43	PF00397	0.401
DOC_WW_Pin1_4	382	387	PF00397	0.469
DOC_WW_Pin1_4	8	13	PF00397	0.431
LIG_14-3-3_CanoR_1	344	349	PF00244	0.612
LIG_14-3-3_CanoR_1	94	100	PF00244	0.377
LIG_BRCT_BRCA1_1	127	131	PF00533	0.409
LIG_BRCT_BRCA1_1	258	262	PF00533	0.374
LIG_BRCT_BRCA1_1	26	30	PF00533	0.348
LIG_EVH1_1	76	80	PF00568	0.377
LIG_FHA_1	38	44	PF00498	0.363
LIG_FHA_1	55	61	PF00498	0.246
LIG_FHA_1	62	68	PF00498	0.196
LIG_FHA_1	9	15	PF00498	0.408
LIG_FHA_1	94	100	PF00498	0.452
LIG_FHA_2	303	309	PF00498	0.418
LIG_FHA_2	337	343	PF00498	0.667
LIG_FHA_2	354	360	PF00498	0.461
LIG_LIR_Apic_2	259	265	PF02991	0.372
LIG_LIR_Apic_2	27	33	PF02991	0.358
LIG_LIR_Gen_1	128	139	PF02991	0.411
LIG_LIR_Gen_1	152	161	PF02991	0.527
LIG_LIR_Gen_1	222	233	PF02991	0.375
LIG_LIR_Gen_1	56	67	PF02991	0.288
LIG_LIR_LC3C_4	321	324	PF02991	0.242
LIG_LIR_Nem_3	128	134	PF02991	0.411
LIG_LIR_Nem_3	222	228	PF02991	0.344
LIG_LIR_Nem_3	307	312	PF02991	0.382
LIG_LIR_Nem_3	56	62	PF02991	0.441
LIG_LIR_Nem_3	86	92	PF02991	0.531
LIG_MLH1_MIPbox_1	258	262	PF16413	0.390
LIG_PTAP_UEV_1	134	139	PF05743	0.369
LIG_SH2_PTP2	113	116	PF00017	0.377
LIG_SH2_STAP1	155	159	PF00017	0.452
LIG_SH2_STAP1	225	229	PF00017	0.377
LIG_SH2_STAT5	113	116	PF00017	0.386
LIG_SH2_STAT5	267	270	PF00017	0.411
LIG_SH2_STAT5	272	275	PF00017	0.394
LIG_SH2_STAT5	302	305	PF00017	0.381
LIG_SH2_STAT5	69	72	PF00017	0.377
LIG_SH3_3	132	138	PF00018	0.369
LIG_SH3_3	320	326	PF00018	0.351
LIG_SH3_3	74	80	PF00018	0.452
LIG_SUMO_SIM_anti_2	40	48	PF11976	0.397
LIG_SUMO_SIM_par_1	341	351	PF11976	0.617
LIG_SUMO_SIM_par_1	40	48	PF11976	0.397
LIG_UBA3_1	229	236	PF00899	0.375
LIG_WRC_WIRS_1	327	332	PF05994	0.523
LIG_WW_2	77	80	PF00397	0.377
MOD_CDK_SPxK_1	29	35	PF00069	0.362
MOD_CDK_SPxxK_3	29	36	PF00069	0.364
MOD_CK1_1	157	163	PF00069	0.407
MOD_CK1_1	274	280	PF00069	0.391
MOD_CK1_1	351	357	PF00069	0.516
MOD_CK1_1	379	385	PF00069	0.643
MOD_CK1_1	81	87	PF00069	0.548
MOD_CK2_1	157	163	PF00069	0.523
MOD_CK2_1	281	287	PF00069	0.380
MOD_CK2_1	336	342	PF00069	0.600
MOD_CK2_1	344	350	PF00069	0.693
MOD_CK2_1	353	359	PF00069	0.546
MOD_Cter_Amidation	330	333	PF01082	0.275
MOD_GlcNHglycan	135	138	PF01048	0.170
MOD_GlcNHglycan	15	18	PF01048	0.239
MOD_GlcNHglycan	350	353	PF01048	0.542
MOD_GlcNHglycan	47	50	PF01048	0.281
MOD_GlcNHglycan	51	54	PF01048	0.311
MOD_GlcNHglycan	80	83	PF01048	0.142
MOD_GSK3_1	270	277	PF00069	0.378
MOD_GSK3_1	344	351	PF00069	0.761
MOD_GSK3_1	377	384	PF00069	0.732
MOD_GSK3_1	45	52	PF00069	0.366
MOD_GSK3_1	78	85	PF00069	0.413
MOD_N-GLC_1	38	43	PF02516	0.450
MOD_NEK2_1	281	286	PF00069	0.467
MOD_NEK2_1	37	42	PF00069	0.443
MOD_NEK2_1	68	73	PF00069	0.369
MOD_NEK2_1	92	97	PF00069	0.381
MOD_NEK2_1	99	104	PF00069	0.452
MOD_PIKK_1	93	99	PF00454	0.452
MOD_PK_1	344	350	PF00069	0.444
MOD_PKA_2	125	131	PF00069	0.365
MOD_PKA_2	377	383	PF00069	0.492
MOD_PKA_2	93	99	PF00069	0.452
MOD_PKB_1	333	341	PF00069	0.422
MOD_Plk_2-3	336	342	PF00069	0.422
MOD_Plk_2-3	353	359	PF00069	0.460
MOD_Plk_4	15	21	PF00069	0.341
MOD_Plk_4	154	160	PF00069	0.403
MOD_Plk_4	246	252	PF00069	0.427
MOD_Plk_4	318	324	PF00069	0.334
MOD_ProDKin_1	157	163	PF00069	0.380
MOD_ProDKin_1	29	35	PF00069	0.481
MOD_ProDKin_1	302	308	PF00069	0.370
MOD_ProDKin_1	38	44	PF00069	0.395
MOD_ProDKin_1	382	388	PF00069	0.470
MOD_ProDKin_1	8	14	PF00069	0.426
TRG_DiLeu_BaLyEn_6	323	328	PF01217	0.271
TRG_ENDOCYTIC_2	155	158	PF00928	0.452
TRG_ENDOCYTIC_2	225	228	PF00928	0.369
TRG_ENDOCYTIC_2	309	312	PF00928	0.377
TRG_ER_diArg_1	288	291	PF00400	0.442
TRG_ER_diArg_1	332	335	PF00400	0.345
TRG_NES_CRM1_1	122	133	PF08389	0.455
TRG_NLS_MonoExtN_4	33	39	PF00514	0.329
TRG_Pf-PMV_PEXEL_1	191	195	PF00026	0.177

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A098DRK7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	22%	83%
A0A0N0P8C0	Leptomonas seymouri	28%	97%
A0A0N1IIY5	Leptomonas seymouri	60%	100%
A0A0S4JSC7	Bodo saltans	26%	100%
A0A1X0NS04	Trypanosomatidae	35%	100%
A0A1X0P2H1	Trypanosomatidae	25%	100%
A0A3S7X6B3	Leishmania donovani	89%	100%
A0A422NI42	Trypanosoma rangeli	37%	100%
A1CJ08	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	25%	97%
A2QY50	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	26%	96%
A4HL26	Leishmania braziliensis	76%	97%
A4I8K7	Leishmania infantum	88%	100%
A5DEF7	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	30%	97%
A5DSB4	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	24%	74%
A6ZRL7	Saccharomyces cerevisiae (strain YJM789)	27%	79%
A7KAL5	Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)	25%	97%
A7TQN1	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	27%	94%
D0AAK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E2RDP2	Canis lupus familiaris	24%	82%
E9B3G7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
P53867	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	79%
Q1E5M9	Coccidioides immitis (strain RS)	26%	90%
Q2HH40	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	24%	87%
Q2U5B0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	27%	95%
Q4U3V5	Cryphonectria parasitica	26%	85%
Q523C3	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	26%	79%
Q59UG3	Candida albicans (strain SC5314 / ATCC MYA-2876)	24%	87%
Q5B7L0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	22%	97%
Q5XH30	Xenopus laevis	26%	86%
Q684M2	Sus scrofa	25%	83%
Q68EP9	Xenopus tropicalis	24%	86%
Q68FJ9	Xenopus laevis	24%	83%
Q6CH28	Yarrowia lipolytica (strain CLIB 122 / E 150)	26%	71%
Q6FP20	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	25%	80%
Q6PZ02	Gallus gallus	21%	99%
Q75KP8	Oryza sativa subsp. japonica	23%	82%
Q811C2	Mus musculus	25%	85%
Q86TL0	Homo sapiens	25%	82%
Q8BGV9	Mus musculus	24%	82%
Q8S929	Arabidopsis thaliana	24%	83%
Q96DT6	Homo sapiens	25%	85%
Q9M1Y0	Arabidopsis thaliana	23%	81%
Q9P373	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
V5AXZ8	Trypanosoma cruzi	37%	100%