Putative trans-splicing factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

trans-splicing factor, putative

Species:

Leishmania major

UniProt:

Q4Q4L0_LEIMA

TriTrypDb:

LmjF.33.0130 , LMJLV39_330006600 , LMJSD75_330006500

Length:

341

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005681	spliceosomal complex	3	2
GO:0005687	U4 snRNP	5	2
GO:0030532	small nuclear ribonucleoprotein complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0046540	U4/U6 x U5 tri-snRNP complex	6	12
GO:0071011	precatalytic spliceosome	4	2
GO:0097525	spliceosomal snRNP complex	4	12
GO:0097526	spliceosomal tri-snRNP complex	5	12
GO:0110165	cellular anatomical entity	1	2
GO:0120114	Sm-like protein family complex	2	12
GO:0140513	nuclear protein-containing complex	2	12
GO:1990904	ribonucleoprotein complex	2	12

Expansion

Sequence features

Q4Q4L0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4L0

Function

Biological processes
Term	Name	Level	Count
GO:0000244	spliceosomal tri-snRNP complex assembly	8	12
GO:0000375	RNA splicing, via transesterification reactions	8	2
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	2
GO:0000387	spliceosomal snRNP assembly	7	12
GO:0000398	mRNA splicing, via spliceosome	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006397	mRNA processing	7	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0008380	RNA splicing	7	2
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0022607	cellular component assembly	4	12
GO:0022618	ribonucleoprotein complex assembly	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043933	protein-containing complex organization	4	12
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0065003	protein-containing complex assembly	5	12
GO:0071704	organic substance metabolic process	2	2
GO:0071826	ribonucleoprotein complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	244	246	PF00675	0.360
CLV_NRD_NRD_1	294	296	PF00675	0.255
CLV_NRD_NRD_1	306	308	PF00675	0.255
CLV_NRD_NRD_1	332	334	PF00675	0.550
CLV_NRD_NRD_1	338	340	PF00675	0.544
CLV_NRD_NRD_1	56	58	PF00675	0.515
CLV_PCSK_FUR_1	304	308	PF00082	0.341
CLV_PCSK_FUR_1	335	339	PF00082	0.582
CLV_PCSK_KEX2_1	244	246	PF00082	0.315
CLV_PCSK_KEX2_1	294	296	PF00082	0.255
CLV_PCSK_KEX2_1	306	308	PF00082	0.255
CLV_PCSK_KEX2_1	332	334	PF00082	0.550
CLV_PCSK_KEX2_1	336	338	PF00082	0.549
CLV_PCSK_KEX2_1	56	58	PF00082	0.515
CLV_PCSK_PC1ET2_1	336	338	PF00082	0.550
CLV_PCSK_PC7_1	240	246	PF00082	0.360
CLV_PCSK_PC7_1	332	338	PF00082	0.559
CLV_PCSK_SKI1_1	126	130	PF00082	0.315
DEG_Nend_UBRbox_2	1	3	PF02207	0.646
DEG_SPOP_SBC_1	160	164	PF00917	0.496
DOC_CYCLIN_RxL_1	272	282	PF00134	0.466
DOC_CYCLIN_yClb5_NLxxxL_5	203	212	PF00134	0.455
DOC_MAPK_gen_1	275	285	PF00069	0.560
DOC_MAPK_gen_1	96	103	PF00069	0.465
DOC_MAPK_JIP1_4	273	279	PF00069	0.466
DOC_MAPK_MEF2A_6	278	287	PF00069	0.560
DOC_MAPK_MEF2A_6	96	103	PF00069	0.541
DOC_MAPK_RevD_3	230	245	PF00069	0.560
DOC_PP2B_LxvP_1	317	320	PF13499	0.480
DOC_USP7_MATH_1	158	162	PF00917	0.455
DOC_WW_Pin1_4	166	171	PF00397	0.541
LIG_14-3-3_CanoR_1	278	284	PF00244	0.462
LIG_14-3-3_CanoR_1	47	51	PF00244	0.490
LIG_14-3-3_CterR_2	337	341	PF00244	0.625
LIG_Actin_WH2_2	310	325	PF00022	0.518
LIG_BRCT_BRCA1_1	107	111	PF00533	0.506
LIG_BRCT_BRCA1_1	217	221	PF00533	0.455
LIG_CtBP_PxDLS_1	170	174	PF00389	0.560
LIG_FHA_1	215	221	PF00498	0.448
LIG_FHA_1	254	260	PF00498	0.458
LIG_FHA_1	282	288	PF00498	0.496
LIG_FHA_1	41	47	PF00498	0.389
LIG_FHA_1	9	15	PF00498	0.717
LIG_FHA_2	11	17	PF00498	0.633
LIG_FHA_2	190	196	PF00498	0.453
LIG_FHA_2	296	302	PF00498	0.541
LIG_LIR_Gen_1	121	131	PF02991	0.489
LIG_LIR_Gen_1	76	84	PF02991	0.421
LIG_LIR_LC3C_4	150	155	PF02991	0.541
LIG_LIR_Nem_3	108	114	PF02991	0.492
LIG_LIR_Nem_3	121	127	PF02991	0.392
LIG_LIR_Nem_3	192	196	PF02991	0.466
LIG_LIR_Nem_3	298	303	PF02991	0.540
LIG_LIR_Nem_3	76	82	PF02991	0.426
LIG_MYND_1	270	274	PF01753	0.479
LIG_RPA_C_Fungi	284	296	PF08784	0.392
LIG_RPA_C_Fungi	93	105	PF08784	0.428
LIG_SH2_CRK	124	128	PF00017	0.307
LIG_SH2_CRK	36	40	PF00017	0.461
LIG_SH2_CRK	79	83	PF00017	0.407
LIG_SH2_NCK_1	36	40	PF00017	0.525
LIG_SH2_SRC	79	82	PF00017	0.495
LIG_SH2_STAP1	107	111	PF00017	0.342
LIG_SH2_STAP1	124	128	PF00017	0.307
LIG_SH2_STAT5	196	199	PF00017	0.293
LIG_SH2_STAT5	50	53	PF00017	0.494
LIG_SH2_STAT5	81	84	PF00017	0.480
LIG_SUMO_SIM_anti_2	150	156	PF11976	0.400
LIG_SUMO_SIM_anti_2	24	32	PF11976	0.603
LIG_SUMO_SIM_par_1	10	16	PF11976	0.623
LIG_TRAF2_1	13	16	PF00917	0.661
LIG_TRAF2_1	169	172	PF00917	0.342
LIG_TRAF2_1	179	182	PF00917	0.293
LIG_TRAF2_1	19	22	PF00917	0.615
LIG_TYR_ITIM	122	127	PF00017	0.307
LIG_TYR_ITIM	34	39	PF00017	0.473
LIG_TYR_ITSM	296	303	PF00017	0.428
MOD_CK1_1	161	167	PF00069	0.358
MOD_CK1_1	243	249	PF00069	0.414
MOD_CK1_1	281	287	PF00069	0.340
MOD_CK1_1	49	55	PF00069	0.450
MOD_CK1_1	83	89	PF00069	0.470
MOD_CK2_1	10	16	PF00069	0.669
MOD_CK2_1	166	172	PF00069	0.342
MOD_CK2_1	189	195	PF00069	0.302
MOD_CK2_1	295	301	PF00069	0.323
MOD_CK2_1	35	41	PF00069	0.431
MOD_CK2_1	56	62	PF00069	0.457
MOD_Cter_Amidation	333	336	PF01082	0.593
MOD_GlcNHglycan	163	166	PF01048	0.451
MOD_GlcNHglycan	223	226	PF01048	0.436
MOD_GlcNHglycan	248	251	PF01048	0.319
MOD_GlcNHglycan	51	54	PF01048	0.401
MOD_GSK3_1	131	138	PF00069	0.426
MOD_GSK3_1	211	218	PF00069	0.304
MOD_GSK3_1	253	260	PF00069	0.333
MOD_GSK3_1	279	286	PF00069	0.356
MOD_GSK3_1	45	52	PF00069	0.385
MOD_GSK3_1	80	87	PF00069	0.479
MOD_N-GLC_1	246	251	PF02516	0.392
MOD_N-GLC_2	207	209	PF02516	0.307
MOD_NEK2_1	118	123	PF00069	0.327
MOD_NEK2_1	211	216	PF00069	0.294
MOD_NEK2_1	221	226	PF00069	0.292
MOD_NEK2_1	279	284	PF00069	0.348
MOD_NEK2_1	45	50	PF00069	0.399
MOD_NEK2_2	105	110	PF00069	0.454
MOD_PIKK_1	147	153	PF00454	0.307
MOD_PIKK_1	215	221	PF00454	0.296
MOD_PK_1	35	41	PF00069	0.431
MOD_PKA_1	56	62	PF00069	0.534
MOD_PKA_2	243	249	PF00069	0.397
MOD_PKA_2	46	52	PF00069	0.507
MOD_PKA_2	56	62	PF00069	0.508
MOD_Plk_1	135	141	PF00069	0.342
MOD_Plk_1	171	177	PF00069	0.392
MOD_Plk_1	23	29	PF00069	0.550
MOD_Plk_1	83	89	PF00069	0.390
MOD_Plk_1	94	100	PF00069	0.342
MOD_Plk_4	118	124	PF00069	0.362
MOD_Plk_4	136	142	PF00069	0.124
MOD_Plk_4	189	195	PF00069	0.304
MOD_Plk_4	228	234	PF00069	0.428
MOD_Plk_4	23	29	PF00069	0.543
MOD_Plk_4	46	52	PF00069	0.409
MOD_Plk_4	84	90	PF00069	0.438
MOD_ProDKin_1	166	172	PF00069	0.428
TRG_DiLeu_BaEn_1	41	46	PF01217	0.384
TRG_DiLeu_BaEn_4	181	187	PF01217	0.428
TRG_DiLeu_BaLyEn_6	199	204	PF01217	0.323
TRG_DiLeu_BaLyEn_6	275	280	PF01217	0.355
TRG_ENDOCYTIC_2	124	127	PF00928	0.307
TRG_ENDOCYTIC_2	300	303	PF00928	0.428
TRG_ENDOCYTIC_2	36	39	PF00928	0.459
TRG_ENDOCYTIC_2	79	82	PF00928	0.409
TRG_ER_diArg_1	306	308	PF00400	0.337
TRG_ER_diArg_1	332	335	PF00400	0.549
TRG_ER_diArg_1	337	339	PF00400	0.547
TRG_ER_diArg_1	55	57	PF00400	0.526
TRG_NLS_MonoCore_2	334	339	PF00514	0.594
TRG_NLS_MonoExtC_3	334	339	PF00514	0.572
TRG_NLS_MonoExtN_4	332	339	PF00514	0.575
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.307
TRG_Pf-PMV_PEXEL_1	56	60	PF00026	0.491

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKZ4	Leptomonas seymouri	90%	100%
A0A0N1PD49	Leptomonas seymouri	21%	80%
A0A0N1PE54	Leptomonas seymouri	23%	72%
A0A0S4J686	Bodo saltans	55%	96%
A0A0S4JJS5	Bodo saltans	22%	81%
A0A1X0NUB3	Trypanosomatidae	23%	78%
A0A1X0P0H6	Trypanosomatidae	68%	96%
A0A3Q8IDJ5	Leishmania donovani	99%	100%
A0A3R7KY21	Trypanosoma rangeli	65%	96%
A0A3S5H6F4	Leishmania donovani	23%	72%
A0A3S5H6V9	Leishmania donovani	22%	77%
A4H602	Leishmania braziliensis	22%	72%
A4H8A6	Leishmania braziliensis	23%	77%
A4HL50	Leishmania braziliensis	95%	100%
A4HUC9	Leishmania infantum	23%	72%
A4HWM6	Leishmania infantum	22%	77%
A4I8M9	Leishmania infantum	98%	100%
C9ZVG2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	71%
D0A4K7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	96%
E9AN29	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	72%
E9AQD3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	76%
E9B3J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O94514	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	21%	69%
Q4QHJ7	Leishmania major	23%	72%
Q5U5C5	Xenopus laevis	29%	68%
Q6NVP6	Xenopus tropicalis	30%	68%
Q7SXM7	Danio rerio	30%	67%
Q8CCF0	Mus musculus	30%	68%
Q8RXN6	Arabidopsis thaliana	31%	70%
Q8WWY3	Homo sapiens	30%	68%
V5BFW3	Trypanosoma cruzi	67%	96%