Nfu_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Scaffold protein Nfu/NifU N terminal/NifU-like domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4Q4F4_LEIMA

TriTrypDb:

LmjF.33.0540 , LMJLV39_330011800 , LMJSD75_330011800

Length:

426

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4Q4F4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q4F4

Function

Biological processes
Term	Name	Level	Count
GO:0006790	sulfur compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0016043	cellular component organization	3	10
GO:0016226	iron-sulfur cluster assembly	4	10
GO:0019538	protein metabolic process	3	2
GO:0022607	cellular component assembly	4	10
GO:0031163	metallo-sulfur cluster assembly	5	10
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	2
GO:0051604	protein maturation	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0071840	cellular component organization or biogenesis	2	10
GO:0097428	protein maturation by iron-sulfur cluster transfer	5	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	10
GO:0005506	iron ion binding	6	10
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0046914	transition metal ion binding	5	10
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051539	4 iron, 4 sulfur cluster binding	4	2
GO:0051540	metal cluster binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	116	120	PF00656	0.327
CLV_C14_Caspase3-7	255	259	PF00656	0.590
CLV_NRD_NRD_1	22	24	PF00675	0.686
CLV_NRD_NRD_1	405	407	PF00675	0.670
CLV_PCSK_KEX2_1	113	115	PF00082	0.344
CLV_PCSK_KEX2_1	22	24	PF00082	0.686
CLV_PCSK_PC1ET2_1	113	115	PF00082	0.485
CLV_PCSK_SKI1_1	45	49	PF00082	0.531
DEG_Nend_UBRbox_1	1	4	PF02207	0.527
DOC_CKS1_1	52	57	PF01111	0.401
DOC_MAPK_gen_1	308	317	PF00069	0.561
DOC_MAPK_MEF2A_6	2	10	PF00069	0.715
DOC_MAPK_RevD_3	98	114	PF00069	0.344
DOC_PP1_RVXF_1	283	290	PF00149	0.531
DOC_PP4_FxxP_1	69	72	PF00568	0.444
DOC_USP7_MATH_1	146	150	PF00917	0.525
DOC_USP7_MATH_1	185	189	PF00917	0.682
DOC_USP7_MATH_1	209	213	PF00917	0.588
DOC_USP7_MATH_1	327	331	PF00917	0.601
DOC_USP7_MATH_1	347	351	PF00917	0.547
DOC_USP7_MATH_2	254	260	PF00917	0.572
DOC_USP7_UBL2_3	403	407	PF12436	0.669
DOC_WW_Pin1_4	180	185	PF00397	0.750
DOC_WW_Pin1_4	194	199	PF00397	0.485
DOC_WW_Pin1_4	360	365	PF00397	0.728
DOC_WW_Pin1_4	384	389	PF00397	0.665
DOC_WW_Pin1_4	51	56	PF00397	0.356
DOC_WW_Pin1_4	84	89	PF00397	0.344
LIG_14-3-3_CanoR_1	28	37	PF00244	0.698
LIG_14-3-3_CanoR_1	305	315	PF00244	0.492
LIG_14-3-3_CanoR_1	337	345	PF00244	0.683
LIG_FHA_1	105	111	PF00498	0.408
LIG_FHA_1	176	182	PF00498	0.527
LIG_FHA_1	229	235	PF00498	0.452
LIG_FHA_1	310	316	PF00498	0.553
LIG_FHA_1	364	370	PF00498	0.753
LIG_FHA_1	44	50	PF00498	0.499
LIG_FHA_2	138	144	PF00498	0.594
LIG_FHA_2	250	256	PF00498	0.667
LIG_FHA_2	311	317	PF00498	0.474
LIG_FHA_2	52	58	PF00498	0.342
LIG_FHA_2	90	96	PF00498	0.429
LIG_GBD_Chelix_1	89	97	PF00786	0.344
LIG_LIR_Apic_2	66	72	PF02991	0.429
LIG_LIR_Apic_2	82	88	PF02991	0.369
LIG_LIR_Gen_1	107	115	PF02991	0.404
LIG_LIR_Gen_1	216	226	PF02991	0.461
LIG_LIR_Gen_1	339	347	PF02991	0.720
LIG_LIR_Gen_1	46	55	PF02991	0.369
LIG_LIR_Nem_3	107	112	PF02991	0.404
LIG_LIR_Nem_3	216	222	PF02991	0.441
LIG_LIR_Nem_3	283	289	PF02991	0.487
LIG_LIR_Nem_3	339	345	PF02991	0.697
LIG_LIR_Nem_3	399	405	PF02991	0.666
LIG_LIR_Nem_3	46	51	PF02991	0.480
LIG_LIR_Nem_3	57	63	PF02991	0.372
LIG_MYND_1	180	184	PF01753	0.671
LIG_Pex14_2	127	131	PF04695	0.519
LIG_PTAP_UEV_1	242	247	PF05743	0.588
LIG_SH2_CRK	402	406	PF00017	0.708
LIG_SH2_STAT5	126	129	PF00017	0.536
LIG_SH2_STAT5	63	66	PF00017	0.401
LIG_SH2_STAT5	85	88	PF00017	0.485
LIG_SH3_3	12	18	PF00018	0.729
LIG_SH3_3	178	184	PF00018	0.635
LIG_SH3_3	240	246	PF00018	0.581
LIG_SUMO_SIM_anti_2	258	265	PF11976	0.487
LIG_SUMO_SIM_anti_2	312	319	PF11976	0.541
LIG_SUMO_SIM_anti_2	7	12	PF11976	0.521
LIG_SUMO_SIM_par_1	258	267	PF11976	0.459
LIG_SUMO_SIM_par_1	312	319	PF11976	0.554
LIG_TRAF2_1	140	143	PF00917	0.644
LIG_TRAF2_1	253	256	PF00917	0.471
MOD_CK1_1	108	114	PF00069	0.444
MOD_CK1_1	197	203	PF00069	0.716
MOD_CK1_1	27	33	PF00069	0.664
MOD_CK1_1	309	315	PF00069	0.474
MOD_CK1_1	336	342	PF00069	0.653
MOD_CK1_1	35	41	PF00069	0.662
MOD_CK1_1	363	369	PF00069	0.765
MOD_CK1_1	389	395	PF00069	0.675
MOD_CK1_1	396	402	PF00069	0.522
MOD_CK1_1	81	87	PF00069	0.363
MOD_CK2_1	137	143	PF00069	0.592
MOD_CK2_1	197	203	PF00069	0.599
MOD_CK2_1	249	255	PF00069	0.625
MOD_CK2_1	337	343	PF00069	0.710
MOD_CK2_1	409	415	PF00069	0.756
MOD_CK2_1	89	95	PF00069	0.429
MOD_GlcNHglycan	226	229	PF01048	0.430
MOD_GlcNHglycan	235	238	PF01048	0.491
MOD_GlcNHglycan	242	246	PF01048	0.560
MOD_GlcNHglycan	328	332	PF01048	0.357
MOD_GlcNHglycan	339	342	PF01048	0.696
MOD_GlcNHglycan	393	396	PF01048	0.758
MOD_GlcNHglycan	398	401	PF01048	0.747
MOD_GlcNHglycan	411	414	PF01048	0.743
MOD_GSK3_1	101	108	PF00069	0.444
MOD_GSK3_1	192	199	PF00069	0.632
MOD_GSK3_1	224	231	PF00069	0.481
MOD_GSK3_1	24	31	PF00069	0.683
MOD_GSK3_1	303	310	PF00069	0.495
MOD_GSK3_1	333	340	PF00069	0.590
MOD_GSK3_1	359	366	PF00069	0.728
MOD_GSK3_1	380	387	PF00069	0.705
MOD_GSK3_1	389	396	PF00069	0.644
MOD_N-GLC_1	79	84	PF02516	0.447
MOD_NEK2_1	129	134	PF00069	0.580
MOD_NEK2_1	224	229	PF00069	0.442
MOD_NEK2_1	268	273	PF00069	0.490
MOD_NEK2_1	393	398	PF00069	0.629
MOD_NEK2_1	6	11	PF00069	0.600
MOD_NEK2_1	79	84	PF00069	0.403
MOD_NEK2_1	89	94	PF00069	0.429
MOD_NEK2_2	303	308	PF00069	0.531
MOD_NEK2_2	43	48	PF00069	0.527
MOD_PIKK_1	146	152	PF00454	0.597
MOD_PIKK_1	197	203	PF00454	0.674
MOD_PKA_2	27	33	PF00069	0.697
MOD_PKA_2	319	325	PF00069	0.500
MOD_PKA_2	336	342	PF00069	0.571
MOD_Plk_1	101	107	PF00069	0.344
MOD_Plk_1	185	191	PF00069	0.704
MOD_Plk_1	192	198	PF00069	0.667
MOD_Plk_1	327	333	PF00069	0.601
MOD_Plk_1	6	12	PF00069	0.483
MOD_Plk_4	105	111	PF00069	0.444
MOD_Plk_4	209	215	PF00069	0.569
MOD_Plk_4	310	316	PF00069	0.551
MOD_Plk_4	32	38	PF00069	0.662
MOD_Plk_4	6	12	PF00069	0.600
MOD_Plk_4	73	79	PF00069	0.406
MOD_Plk_4	81	87	PF00069	0.411
MOD_Plk_4	89	95	PF00069	0.405
MOD_ProDKin_1	180	186	PF00069	0.751
MOD_ProDKin_1	194	200	PF00069	0.483
MOD_ProDKin_1	360	366	PF00069	0.728
MOD_ProDKin_1	384	390	PF00069	0.659
MOD_ProDKin_1	51	57	PF00069	0.356
MOD_ProDKin_1	84	90	PF00069	0.344
MOD_SUMO_rev_2	216	224	PF00179	0.439
TRG_ENDOCYTIC_2	126	129	PF00928	0.546
TRG_ENDOCYTIC_2	402	405	PF00928	0.706
TRG_ER_diArg_1	21	23	PF00400	0.688

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6M8	Leptomonas seymouri	63%	90%
A0A0S4J2M4	Bodo saltans	40%	94%
A0A3S7X6H5	Leishmania donovani	92%	95%
A4HL91	Leishmania braziliensis	76%	99%
A4I8R7	Leishmania infantum	92%	95%
D0A4Q8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E9B3N3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5B2F6	Trypanosoma cruzi	53%	94%