Putative peptidase M20/M25/M40

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytosolic nonspecific dipeptidase, putative

Species:

Leishmania major

UniProt:

Q4Q426_LEIMA

TriTrypDb:

LmjF.33.1610 , LMJLV39_330024700 , LMJSD75_330024400

Length:

472

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 33

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 23
NetGPI	no	yes: 0, no: 23

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q426

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q426

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0008152	metabolic process	1	4
GO:0019538	protein metabolic process	3	4
GO:0043170	macromolecule metabolic process	3	4
GO:0044238	primary metabolic process	2	4
GO:0071704	organic substance metabolic process	2	4
GO:1901564	organonitrogen compound metabolic process	3	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	24
GO:0008233	peptidase activity	3	4
GO:0016787	hydrolase activity	2	24
GO:0140096	catalytic activity, acting on a protein	2	4
GO:0005488	binding	1	1
GO:0005506	iron ion binding	6	1
GO:0016491	oxidoreductase activity	2	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0046914	transition metal ion binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	9	11	PF00675	0.526
CLV_PCSK_SKI1_1	210	214	PF00082	0.387
CLV_PCSK_SKI1_1	243	247	PF00082	0.347
CLV_PCSK_SKI1_1	256	260	PF00082	0.495
CLV_PCSK_SKI1_1	453	457	PF00082	0.346
CLV_PCSK_SKI1_1	50	54	PF00082	0.555
DEG_APCC_DBOX_1	242	250	PF00400	0.347
DOC_CYCLIN_RxL_1	240	250	PF00134	0.334
DOC_CYCLIN_RxL_1	253	261	PF00134	0.266
DOC_CYCLIN_RxL_1	448	460	PF00134	0.409
DOC_MAPK_gen_1	181	189	PF00069	0.409
DOC_MAPK_HePTP_8	302	314	PF00069	0.308
DOC_MAPK_MEF2A_6	183	191	PF00069	0.361
DOC_MAPK_MEF2A_6	305	314	PF00069	0.308
DOC_MAPK_MEF2A_6	330	339	PF00069	0.353
DOC_MAPK_MEF2A_6	77	85	PF00069	0.424
DOC_PP1_RVXF_1	254	260	PF00149	0.266
DOC_PP1_RVXF_1	365	371	PF00149	0.372
DOC_PP1_RVXF_1	451	458	PF00149	0.339
DOC_PP2B_LxvP_1	339	342	PF13499	0.293
DOC_PP4_FxxP_1	307	310	PF00568	0.409
DOC_USP7_MATH_1	141	145	PF00917	0.431
DOC_WW_Pin1_4	32	37	PF00397	0.421
DOC_WW_Pin1_4	378	383	PF00397	0.299
DOC_WW_Pin1_4	467	472	PF00397	0.441
DOC_WW_Pin1_4	75	80	PF00397	0.413
LIG_14-3-3_CanoR_1	330	336	PF00244	0.363
LIG_Actin_WH2_2	321	338	PF00022	0.387
LIG_BIR_II_1	1	5	PF00653	0.510
LIG_BRCT_BRCA1_1	167	171	PF00533	0.352
LIG_BRCT_BRCA1_1	34	38	PF00533	0.510
LIG_BRCT_BRCA1_1	411	415	PF00533	0.224
LIG_Clathr_ClatBox_1	71	75	PF01394	0.498
LIG_FHA_1	204	210	PF00498	0.351
LIG_FHA_1	211	217	PF00498	0.351
LIG_FHA_1	236	242	PF00498	0.392
LIG_FHA_1	252	258	PF00498	0.363
LIG_FHA_1	379	385	PF00498	0.272
LIG_FHA_1	42	48	PF00498	0.467
LIG_FHA_1	429	435	PF00498	0.347
LIG_FHA_1	454	460	PF00498	0.368
LIG_FHA_1	51	57	PF00498	0.424
LIG_FHA_1	76	82	PF00498	0.403
LIG_FHA_2	219	225	PF00498	0.363
LIG_LIR_Gen_1	133	142	PF02991	0.391
LIG_LIR_Gen_1	261	271	PF02991	0.394
LIG_LIR_Gen_1	3	13	PF02991	0.426
LIG_LIR_Gen_1	412	423	PF02991	0.228
LIG_LIR_Gen_1	445	452	PF02991	0.359
LIG_LIR_Gen_1	49	59	PF02991	0.412
LIG_LIR_Gen_1	68	74	PF02991	0.446
LIG_LIR_Gen_1	78	86	PF02991	0.355
LIG_LIR_Nem_3	133	139	PF02991	0.362
LIG_LIR_Nem_3	261	266	PF02991	0.344
LIG_LIR_Nem_3	3	9	PF02991	0.436
LIG_LIR_Nem_3	445	449	PF02991	0.359
LIG_LIR_Nem_3	49	55	PF02991	0.455
LIG_LIR_Nem_3	78	83	PF02991	0.353
LIG_LRP6_Inhibitor_1	324	330	PF00058	0.430
LIG_MYND_1	105	109	PF01753	0.351
LIG_MYND_1	382	386	PF01753	0.359
LIG_PCNA_yPIPBox_3	421	434	PF02747	0.290
LIG_Pex14_2	282	286	PF04695	0.376
LIG_Pex14_2	38	42	PF04695	0.537
LIG_PTB_Apo_2	358	365	PF02174	0.312
LIG_PTB_Phospho_1	358	364	PF10480	0.327
LIG_SH2_CRK	400	404	PF00017	0.296
LIG_SH2_PTP2	28	31	PF00017	0.409
LIG_SH2_SRC	28	31	PF00017	0.422
LIG_SH2_STAP1	200	204	PF00017	0.363
LIG_SH2_STAT5	263	266	PF00017	0.397
LIG_SH2_STAT5	269	272	PF00017	0.367
LIG_SH2_STAT5	28	31	PF00017	0.405
LIG_SH3_1	103	109	PF00018	0.351
LIG_SH3_3	103	109	PF00018	0.352
LIG_SH3_3	254	260	PF00018	0.391
LIG_SH3_3	356	362	PF00018	0.277
LIG_SH3_3	369	375	PF00018	0.234
LIG_SH3_3	430	436	PF00018	0.281
LIG_SUMO_SIM_anti_2	143	149	PF11976	0.296
LIG_SUMO_SIM_par_1	157	163	PF11976	0.347
LIG_TRAF2_1	164	167	PF00917	0.343
LIG_TRAF2_2	32	37	PF00917	0.365
LIG_TYR_ITIM	198	203	PF00017	0.296
LIG_UBA3_1	55	60	PF00899	0.344
MOD_CDK_SPK_2	467	472	PF00069	0.444
MOD_CDK_SPxxK_3	378	385	PF00069	0.308
MOD_CK1_1	140	146	PF00069	0.293
MOD_CK1_1	225	231	PF00069	0.386
MOD_CK1_1	273	279	PF00069	0.401
MOD_CK2_1	218	224	PF00069	0.363
MOD_GlcNHglycan	139	142	PF01048	0.299
MOD_GlcNHglycan	166	170	PF01048	0.363
MOD_GlcNHglycan	194	197	PF01048	0.352
MOD_GlcNHglycan	227	230	PF01048	0.351
MOD_GlcNHglycan	292	295	PF01048	0.417
MOD_GSK3_1	137	144	PF00069	0.450
MOD_GSK3_1	218	225	PF00069	0.464
MOD_GSK3_1	308	315	PF00069	0.485
MOD_GSK3_1	331	338	PF00069	0.363
MOD_GSK3_1	453	460	PF00069	0.376
MOD_GSK3_1	46	53	PF00069	0.472
MOD_N-GLC_1	198	203	PF02516	0.471
MOD_N-GLC_1	32	37	PF02516	0.432
MOD_NEK2_1	198	203	PF00069	0.383
MOD_NEK2_1	205	210	PF00069	0.315
MOD_NEK2_1	222	227	PF00069	0.342
MOD_NEK2_1	312	317	PF00069	0.396
MOD_NEK2_1	335	340	PF00069	0.293
MOD_NEK2_1	409	414	PF00069	0.307
MOD_NEK2_1	428	433	PF00069	0.383
MOD_NEK2_1	457	462	PF00069	0.453
MOD_PIKK_1	198	204	PF00454	0.517
MOD_PIKK_1	348	354	PF00454	0.438
MOD_Plk_1	17	23	PF00069	0.409
MOD_Plk_1	198	204	PF00069	0.469
MOD_Plk_2-3	167	173	PF00069	0.308
MOD_Plk_4	143	149	PF00069	0.409
MOD_Plk_4	17	23	PF00069	0.402
MOD_Plk_4	91	97	PF00069	0.422
MOD_ProDKin_1	32	38	PF00069	0.425
MOD_ProDKin_1	378	384	PF00069	0.299
MOD_ProDKin_1	75	81	PF00069	0.410
MOD_SUMO_rev_2	247	254	PF00179	0.441
MOD_SUMO_rev_2	377	386	PF00179	0.237
TRG_ENDOCYTIC_2	136	139	PF00928	0.424
TRG_ENDOCYTIC_2	200	203	PF00928	0.363
TRG_ENDOCYTIC_2	263	266	PF00928	0.397
TRG_ENDOCYTIC_2	28	31	PF00928	0.404
TRG_ENDOCYTIC_2	69	72	PF00928	0.408
TRG_ENDOCYTIC_2	98	101	PF00928	0.345
TRG_Pf-PMV_PEXEL_1	243	247	PF00026	0.296

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1Q9	Leptomonas seymouri	82%	100%
A0A0N1PBD8	Leptomonas seymouri	74%	100%
A0A0S4JJE7	Bodo saltans	58%	100%
A0A1X0P1Q0	Trypanosomatidae	68%	100%
A0A381MQ29	Leishmania infantum	74%	100%
A0A3Q8IFP2	Leishmania donovani	74%	83%
A0A3R7KSZ4	Trypanosoma rangeli	64%	100%
A0A3S7X4P6	Leishmania donovani	74%	100%
A0A3S7X6U2	Leishmania donovani	94%	100%
A4HJG9	Leishmania braziliensis	74%	100%
A4HJH1	Leishmania braziliensis	74%	100%
A4HJI4	Leishmania braziliensis	74%	100%
A4HLM2	Leishmania braziliensis	86%	100%
A4I933	Leishmania infantum	94%	100%
B2KZE7	Corynebacterium striatum	28%	100%
C9ZQ48	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	99%
D0A536	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	99%
E9B1Y8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
E9B201	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	100%
E9B3Z8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
P43616	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	98%
Q3ZC84	Bos taurus	26%	99%
Q4Q660	Leishmania major	74%	82%
Q4Q673	Leishmania major	74%	82%
Q5R432	Pongo abelii	26%	99%
Q6Q0N1	Rattus norvegicus	27%	99%
Q96KP4	Homo sapiens	27%	99%
Q9D1A2	Mus musculus	26%	99%
V5BHA2	Trypanosoma cruzi	63%	99%