Histone-lysine N-methyltransferase, H3 lysine-79 specific

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Histone methylation protein DOT1, putative

Species:

Leishmania major

UniProt:

Q4Q409_LEIMA

TriTrypDb:

LmjF.33.1790 , LMJLV39_330027200 , LMJSD75_330026800

Length:

253

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0016020	membrane	2	2

Expansion

Sequence features

Q4Q409

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q409

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	2
GO:0000077	DNA damage checkpoint signaling	5	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006325	chromatin organization	4	12
GO:0006479	protein methylation	4	12
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0007165	signal transduction	2	2
GO:0008152	metabolic process	1	12
GO:0008213	protein alkylation	5	12
GO:0009987	cellular process	1	12
GO:0010564	regulation of cell cycle process	5	2
GO:0010948	negative regulation of cell cycle process	6	2
GO:0016043	cellular component organization	3	12
GO:0016570	histone modification	5	12
GO:0016571	histone methylation	5	12
GO:0018022	peptidyl-lysine methylation	5	12
GO:0018193	peptidyl-amino acid modification	5	12
GO:0018205	peptidyl-lysine modification	6	12
GO:0019538	protein metabolic process	3	12
GO:0031570	DNA integrity checkpoint signaling	5	2
GO:0032259	methylation	2	12
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034729	histone H3-K79 methylation	7	12
GO:0034968	histone lysine methylation	6	12
GO:0035556	intracellular signal transduction	3	2
GO:0036211	protein modification process	4	12
GO:0042770	signal transduction in response to DNA damage	4	2
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0045786	negative regulation of cell cycle	5	2
GO:0046483	heterocycle metabolic process	3	2
GO:0048519	negative regulation of biological process	3	2
GO:0048523	negative regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0050896	response to stimulus	1	2
GO:0051098	regulation of binding	3	2
GO:0051101	regulation of DNA binding	4	2
GO:0051716	cellular response to stimulus	2	2
GO:0051726	regulation of cell cycle	4	12
GO:0065007	biological regulation	1	12
GO:0065009	regulation of molecular function	2	2
GO:0071704	organic substance metabolic process	2	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901987	regulation of cell cycle phase transition	6	2
GO:1901988	negative regulation of cell cycle phase transition	7	2
GO:2000677	regulation of transcription regulatory region DNA binding	5	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008276	protein methyltransferase activity	3	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016278	lysine N-methyltransferase activity	6	12
GO:0016279	protein-lysine N-methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0018024	obsolete histone lysine N-methyltransferase activity	5	12
GO:0031151	histone H3K79 methyltransferase activity	6	12
GO:0042054	histone methyltransferase activity	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	217	219	PF00675	0.299
CLV_NRD_NRD_1	86	88	PF00675	0.628
CLV_PCSK_KEX2_1	149	151	PF00082	0.339
CLV_PCSK_KEX2_1	88	90	PF00082	0.651
CLV_PCSK_PC1ET2_1	149	151	PF00082	0.339
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.721
CLV_PCSK_SKI1_1	105	109	PF00082	0.300
CLV_PCSK_SKI1_1	149	153	PF00082	0.348
CLV_PCSK_SKI1_1	89	93	PF00082	0.699
DEG_Nend_UBRbox_3	1	3	PF02207	0.532
DOC_CYCLIN_RxL_1	86	94	PF00134	0.714
DOC_MAPK_gen_1	127	136	PF00069	0.305
DOC_MAPK_gen_1	161	167	PF00069	0.479
DOC_MAPK_gen_1	177	185	PF00069	0.258
DOC_MAPK_gen_1	231	238	PF00069	0.637
DOC_MAPK_MEF2A_6	127	136	PF00069	0.305
DOC_MAPK_MEF2A_6	179	187	PF00069	0.349
DOC_PP1_RVXF_1	103	109	PF00149	0.342
DOC_USP7_UBL2_3	219	223	PF12436	0.411
DOC_WW_Pin1_4	17	22	PF00397	0.609
LIG_14-3-3_CanoR_1	231	236	PF00244	0.581
LIG_Actin_WH2_2	134	151	PF00022	0.203
LIG_BRCT_BRCA1_1	155	159	PF00533	0.411
LIG_BRCT_BRCA1_1	189	193	PF00533	0.266
LIG_BRCT_BRCA1_2	155	161	PF00533	0.331
LIG_deltaCOP1_diTrp_1	241	248	PF00928	0.488
LIG_EVH1_2	21	25	PF00568	0.612
LIG_FHA_1	200	206	PF00498	0.281
LIG_FHA_1	74	80	PF00498	0.537
LIG_LIR_Nem_3	10	16	PF02991	0.583
LIG_LIR_Nem_3	245	251	PF02991	0.505
LIG_PCNA_PIPBox_1	29	38	PF02747	0.668
LIG_Pex14_2	187	191	PF04695	0.315
LIG_Pex14_2	238	242	PF04695	0.599
LIG_SH2_CRK	13	17	PF00017	0.724
LIG_SH2_NCK_1	155	159	PF00017	0.331
LIG_SH2_SRC	249	252	PF00017	0.510
LIG_SH2_STAP1	155	159	PF00017	0.331
LIG_SH2_STAT5	249	252	PF00017	0.448
LIG_SH2_STAT5	3	6	PF00017	0.656
LIG_SH3_3	24	30	PF00018	0.578
LIG_SUMO_SIM_anti_2	180	188	PF11976	0.331
LIG_TRAF2_1	143	146	PF00917	0.411
LIG_TYR_ITIM	153	158	PF00017	0.331
LIG_TYR_ITIM	31	36	PF00017	0.679
LIG_UBA3_1	79	88	PF00899	0.535
MOD_CK2_1	157	163	PF00069	0.331
MOD_CK2_1	39	45	PF00069	0.649
MOD_CK2_1	91	97	PF00069	0.589
MOD_Cter_Amidation	85	88	PF01082	0.540
MOD_GlcNHglycan	112	115	PF01048	0.328
MOD_GSK3_1	153	160	PF00069	0.362
MOD_GSK3_1	181	188	PF00069	0.371
MOD_GSK3_1	91	98	PF00069	0.645
MOD_N-GLC_2	117	119	PF02516	0.266
MOD_NEK2_1	123	128	PF00069	0.266
MOD_NEK2_1	16	21	PF00069	0.573
MOD_NEK2_1	187	192	PF00069	0.283
MOD_NEK2_1	225	230	PF00069	0.522
MOD_NEK2_1	91	96	PF00069	0.583
MOD_NEK2_2	226	231	PF00069	0.470
MOD_PIKK_1	95	101	PF00454	0.631
MOD_PK_1	5	11	PF00069	0.650
MOD_Plk_1	49	55	PF00069	0.622
MOD_Plk_4	181	187	PF00069	0.290
MOD_ProDKin_1	17	23	PF00069	0.609
MOD_SUMO_for_1	178	181	PF00179	0.309
MOD_SUMO_rev_2	124	129	PF00179	0.331
MOD_SUMO_rev_2	217	225	PF00179	0.411
TRG_DiLeu_BaLyEn_6	27	32	PF01217	0.670
TRG_ENDOCYTIC_2	13	16	PF00928	0.699
TRG_ENDOCYTIC_2	155	158	PF00928	0.411
TRG_ENDOCYTIC_2	33	36	PF00928	0.560
TRG_ER_diArg_1	230	233	PF00400	0.610
TRG_NES_CRM1_1	110	121	PF08389	0.266
TRG_Pf-PMV_PEXEL_1	218	222	PF00026	0.280
TRG_Pf-PMV_PEXEL_1	34	38	PF00026	0.668
TRG_Pf-PMV_PEXEL_1	89	93	PF00026	0.725

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMC9	Leptomonas seymouri	77%	84%
A0A0S4JJM1	Bodo saltans	46%	74%
A0A1X0P3M1	Trypanosomatidae	52%	100%
A0A3S5H7U7	Leishmania donovani	98%	100%
A0A422N3B5	Trypanosoma rangeli	52%	100%
A4HLN9	Leishmania braziliensis	92%	100%
A4I974	Leishmania infantum	98%	100%
D0A6D6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	91%
E9B415	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5B8S7	Trypanosoma cruzi	51%	100%