2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diphthamide biosynthesis enzyme Dph1/Dph2 domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4Q3U6_LEIMA

TriTrypDb:

LmjF.33.2410 , LMJLV39_330034900 , LMJSD75_330034200

Length:

666

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q3U6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3U6

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	11
GO:0006448	regulation of translational elongation	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009889	regulation of biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0010468	regulation of gene expression	5	11
GO:0010556	regulation of macromolecule biosynthetic process	5	11
GO:0010608	post-transcriptional regulation of gene expression	6	11
GO:0017182	peptidyl-diphthamide metabolic process	7	11
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	11
GO:0018193	peptidyl-amino acid modification	5	11
GO:0018202	peptidyl-histidine modification	6	11
GO:0019222	regulation of metabolic process	3	11
GO:0019538	protein metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	11
GO:0031326	regulation of cellular biosynthetic process	5	11
GO:0034248	regulation of amide metabolic process	5	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0050789	regulation of biological process	2	11
GO:0050794	regulation of cellular process	3	11
GO:0051171	regulation of nitrogen compound metabolic process	4	11
GO:0051246	regulation of protein metabolic process	5	11
GO:0060255	regulation of macromolecule metabolic process	4	11
GO:0065007	biological regulation	1	11
GO:0071704	organic substance metabolic process	2	11
GO:0080090	regulation of primary metabolic process	4	11
GO:1900247	regulation of cytoplasmic translational elongation	8	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016740	transferase activity	2	11
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	11
GO:0090560	2-(3-amino-3-carboxypropyl)histidine synthase activity	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	507	511	PF00656	0.534
CLV_MEL_PAP_1	627	633	PF00089	0.677
CLV_NRD_NRD_1	173	175	PF00675	0.468
CLV_NRD_NRD_1	421	423	PF00675	0.336
CLV_PCSK_KEX2_1	173	175	PF00082	0.433
CLV_PCSK_KEX2_1	39	41	PF00082	0.633
CLV_PCSK_KEX2_1	421	423	PF00082	0.273
CLV_PCSK_KEX2_1	467	469	PF00082	0.282
CLV_PCSK_KEX2_1	508	510	PF00082	0.362
CLV_PCSK_KEX2_1	556	558	PF00082	0.538
CLV_PCSK_PC1ET2_1	39	41	PF00082	0.496
CLV_PCSK_PC1ET2_1	467	469	PF00082	0.282
CLV_PCSK_PC1ET2_1	508	510	PF00082	0.362
CLV_PCSK_PC1ET2_1	556	558	PF00082	0.563
CLV_PCSK_PC7_1	463	469	PF00082	0.282
CLV_PCSK_SKI1_1	2	6	PF00082	0.400
CLV_PCSK_SKI1_1	76	80	PF00082	0.601
DEG_Kelch_Keap1_1	269	274	PF01344	0.633
DEG_Nend_UBRbox_1	1	4	PF02207	0.366
DEG_SPOP_SBC_1	267	271	PF00917	0.626
DEG_SPOP_SBC_1	287	291	PF00917	0.671
DEG_SPOP_SBC_1	543	547	PF00917	0.320
DOC_MAPK_gen_1	467	474	PF00069	0.482
DOC_MAPK_MEF2A_6	434	443	PF00069	0.482
DOC_MAPK_MEF2A_6	87	94	PF00069	0.232
DOC_MAPK_NFAT4_5	87	95	PF00069	0.232
DOC_PP2B_PxIxI_1	205	211	PF00149	0.411
DOC_PP4_FxxP_1	512	515	PF00568	0.496
DOC_USP7_MATH_1	124	128	PF00917	0.496
DOC_USP7_MATH_1	259	263	PF00917	0.679
DOC_USP7_MATH_1	294	298	PF00917	0.539
DOC_USP7_MATH_1	308	312	PF00917	0.509
DOC_USP7_MATH_1	342	346	PF00917	0.535
DOC_USP7_MATH_1	525	529	PF00917	0.585
DOC_USP7_MATH_1	561	565	PF00917	0.519
DOC_USP7_MATH_1	72	76	PF00917	0.629
DOC_WW_Pin1_4	290	295	PF00397	0.510
DOC_WW_Pin1_4	515	520	PF00397	0.482
DOC_WW_Pin1_4	631	636	PF00397	0.562
LIG_14-3-3_CanoR_1	173	183	PF00244	0.471
LIG_14-3-3_CanoR_1	226	236	PF00244	0.511
LIG_14-3-3_CanoR_1	245	250	PF00244	0.665
LIG_14-3-3_CanoR_1	286	296	PF00244	0.635
LIG_14-3-3_CanoR_1	434	443	PF00244	0.459
LIG_14-3-3_CanoR_1	587	595	PF00244	0.672
LIG_14-3-3_CanoR_1	76	81	PF00244	0.666
LIG_BIR_III_2	574	578	PF00653	0.529
LIG_BIR_III_4	155	159	PF00653	0.376
LIG_deltaCOP1_diTrp_1	274	284	PF00928	0.492
LIG_FHA_1	146	152	PF00498	0.612
LIG_FHA_1	164	170	PF00498	0.415
LIG_FHA_1	214	220	PF00498	0.473
LIG_FHA_1	453	459	PF00498	0.491
LIG_FHA_2	227	233	PF00498	0.583
LIG_FHA_2	530	536	PF00498	0.539
LIG_FHA_2	571	577	PF00498	0.670
LIG_GBD_Chelix_1	175	183	PF00786	0.420
LIG_LIR_Apic_2	510	515	PF02991	0.497
LIG_LIR_Gen_1	299	309	PF02991	0.525
LIG_LIR_Gen_1	321	331	PF02991	0.536
LIG_LIR_Gen_1	490	499	PF02991	0.477
LIG_LIR_Gen_1	545	555	PF02991	0.420
LIG_LIR_Nem_3	238	242	PF02991	0.391
LIG_LIR_Nem_3	299	305	PF02991	0.482
LIG_LIR_Nem_3	321	326	PF02991	0.517
LIG_LIR_Nem_3	449	454	PF02991	0.514
LIG_LIR_Nem_3	469	473	PF02991	0.505
LIG_LIR_Nem_3	490	495	PF02991	0.477
LIG_LIR_Nem_3	545	551	PF02991	0.414
LIG_LIR_Nem_3	621	627	PF02991	0.541
LIG_MAD2	286	294	PF02301	0.435
LIG_NRBOX	457	463	PF00104	0.496
LIG_PCNA_PIPBox_1	613	622	PF02747	0.622
LIG_PCNA_yPIPBox_3	613	622	PF02747	0.574
LIG_PTB_Apo_2	351	358	PF02174	0.426
LIG_Rb_pABgroove_1	614	622	PF01858	0.521
LIG_RPA_C_Fungi	168	180	PF08784	0.463
LIG_SH2_PTP2	136	139	PF00017	0.447
LIG_SH2_PTP2	624	627	PF00017	0.635
LIG_SH2_SRC	32	35	PF00017	0.623
LIG_SH2_STAP1	544	548	PF00017	0.434
LIG_SH2_STAP1	620	624	PF00017	0.596
LIG_SH2_STAT3	499	502	PF00017	0.398
LIG_SH2_STAT5	113	116	PF00017	0.432
LIG_SH2_STAT5	136	139	PF00017	0.335
LIG_SH2_STAT5	302	305	PF00017	0.383
LIG_SH2_STAT5	32	35	PF00017	0.623
LIG_SH2_STAT5	335	338	PF00017	0.460
LIG_SH2_STAT5	473	476	PF00017	0.345
LIG_SH2_STAT5	544	547	PF00017	0.505
LIG_SH2_STAT5	624	627	PF00017	0.610
LIG_SH3_3	200	206	PF00018	0.568
LIG_SH3_3	279	285	PF00018	0.517
LIG_SH3_3	492	498	PF00018	0.447
LIG_SH3_3	54	60	PF00018	0.762
LIG_SH3_3	80	86	PF00018	0.682
LIG_Sin3_3	165	172	PF02671	0.327
LIG_SUMO_SIM_anti_2	14	20	PF11976	0.454
LIG_SUMO_SIM_par_1	164	170	PF11976	0.507
LIG_SUMO_SIM_par_1	521	528	PF11976	0.378
LIG_TRAF2_1	59	62	PF00917	0.539
MOD_CK1_1	127	133	PF00069	0.337
MOD_CK1_1	144	150	PF00069	0.484
MOD_CK1_1	269	275	PF00069	0.602
MOD_CK1_1	289	295	PF00069	0.687
MOD_CK1_1	377	383	PF00069	0.557
MOD_CK1_1	500	506	PF00069	0.398
MOD_CK1_1	598	604	PF00069	0.734
MOD_CK2_1	225	231	PF00069	0.600
MOD_CK2_1	268	274	PF00069	0.589
MOD_CK2_1	515	521	PF00069	0.328
MOD_Cter_Amidation	465	468	PF01082	0.335
MOD_GlcNHglycan	143	146	PF01048	0.554
MOD_GlcNHglycan	176	179	PF01048	0.536
MOD_GlcNHglycan	196	199	PF01048	0.339
MOD_GlcNHglycan	260	264	PF01048	0.694
MOD_GlcNHglycan	271	274	PF01048	0.643
MOD_GlcNHglycan	311	314	PF01048	0.413
MOD_GlcNHglycan	376	379	PF01048	0.522
MOD_GlcNHglycan	381	384	PF01048	0.487
MOD_GlcNHglycan	395	398	PF01048	0.409
MOD_GlcNHglycan	436	439	PF01048	0.354
MOD_GlcNHglycan	527	530	PF01048	0.355
MOD_GlcNHglycan	590	593	PF01048	0.596
MOD_GlcNHglycan	600	603	PF01048	0.618
MOD_GlcNHglycan	662	665	PF01048	0.793
MOD_GlcNHglycan	87	90	PF01048	0.600
MOD_GSK3_1	141	148	PF00069	0.417
MOD_GSK3_1	227	234	PF00069	0.516
MOD_GSK3_1	286	293	PF00069	0.621
MOD_GSK3_1	353	360	PF00069	0.403
MOD_GSK3_1	377	384	PF00069	0.567
MOD_GSK3_1	429	436	PF00069	0.409
MOD_GSK3_1	525	532	PF00069	0.386
MOD_GSK3_1	561	568	PF00069	0.560
MOD_GSK3_1	72	79	PF00069	0.619
MOD_LATS_1	74	80	PF00433	0.609
MOD_N-GLC_1	353	358	PF02516	0.403
MOD_N-GLC_1	368	373	PF02516	0.515
MOD_NEK2_1	126	131	PF00069	0.340
MOD_NEK2_1	141	146	PF00069	0.415
MOD_NEK2_1	288	293	PF00069	0.648
MOD_NEK2_1	353	358	PF00069	0.437
MOD_NEK2_1	374	379	PF00069	0.445
MOD_NEK2_1	442	447	PF00069	0.418
MOD_PIKK_1	452	458	PF00454	0.418
MOD_PIKK_1	46	52	PF00454	0.693
MOD_PIKK_1	529	535	PF00454	0.567
MOD_PIKK_1	609	615	PF00454	0.627
MOD_PK_1	245	251	PF00069	0.669
MOD_PKA_2	127	133	PF00069	0.335
MOD_PKA_2	181	187	PF00069	0.560
MOD_PKA_2	225	231	PF00069	0.562
MOD_PKA_2	433	439	PF00069	0.354
MOD_PKA_2	500	506	PF00069	0.398
MOD_PKA_2	561	567	PF00069	0.592
MOD_PKA_2	586	592	PF00069	0.664
MOD_PKA_2	595	601	PF00069	0.600
MOD_Plk_1	163	169	PF00069	0.384
MOD_Plk_1	231	237	PF00069	0.528
MOD_Plk_1	353	359	PF00069	0.434
MOD_Plk_1	96	102	PF00069	0.335
MOD_Plk_4	163	169	PF00069	0.479
MOD_ProDKin_1	290	296	PF00069	0.504
MOD_ProDKin_1	515	521	PF00069	0.335
MOD_ProDKin_1	631	637	PF00069	0.564
MOD_SUMO_rev_2	34	41	PF00179	0.650
MOD_SUMO_rev_2	564	568	PF00179	0.483
TRG_DiLeu_BaLyEn_6	550	555	PF01217	0.384
TRG_ENDOCYTIC_2	302	305	PF00928	0.435
TRG_ENDOCYTIC_2	323	326	PF00928	0.436
TRG_ENDOCYTIC_2	328	331	PF00928	0.438
TRG_ENDOCYTIC_2	451	454	PF00928	0.381
TRG_ENDOCYTIC_2	624	627	PF00928	0.635
TRG_ER_diArg_1	173	175	PF00400	0.448
TRG_ER_diArg_1	179	182	PF00400	0.440
TRG_ER_diArg_1	421	424	PF00400	0.361

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8D8	Leptomonas seymouri	57%	86%
A0A1X0P3T2	Trypanosomatidae	38%	100%
A0A3S5ISG6	Trypanosoma rangeli	40%	100%
A0A3S7X6Z1	Leishmania donovani	92%	100%
A4HLV3	Leishmania braziliensis	73%	87%
D0A670	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AHQ9	Leishmania infantum	91%	86%
E9B480	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
V5BP75	Trypanosoma cruzi	39%	100%