ThiF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4Q3S1_LEIMA

TriTrypDb:

LmjF.33.2650 , LMJLV39_330037600 , LMJSD75_330036900

Length:

787

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q3S1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3S1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	6
GO:0019538	protein metabolic process	3	6
GO:0032446	protein modification by small protein conjugation	6	6
GO:0036211	protein modification process	4	6
GO:0043170	macromolecule metabolic process	3	6
GO:0043412	macromolecule modification	4	6
GO:0044238	primary metabolic process	2	6
GO:0045116	protein neddylation	7	6
GO:0070647	protein modification by small protein conjugation or removal	5	6
GO:0071704	organic substance metabolic process	2	6
GO:1901564	organonitrogen compound metabolic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	12
GO:0016874	ligase activity	2	12
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	12
GO:0019781	NEDD8 activating enzyme activity	3	6
GO:0140096	catalytic activity, acting on a protein	2	12
GO:0140657	ATP-dependent activity	1	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	301	305	PF00656	0.539
CLV_NRD_NRD_1	145	147	PF00675	0.480
CLV_NRD_NRD_1	323	325	PF00675	0.465
CLV_NRD_NRD_1	365	367	PF00675	0.483
CLV_NRD_NRD_1	460	462	PF00675	0.515
CLV_NRD_NRD_1	558	560	PF00675	0.566
CLV_NRD_NRD_1	642	644	PF00675	0.495
CLV_PCSK_KEX2_1	145	147	PF00082	0.497
CLV_PCSK_KEX2_1	323	325	PF00082	0.465
CLV_PCSK_KEX2_1	334	336	PF00082	0.516
CLV_PCSK_KEX2_1	365	367	PF00082	0.470
CLV_PCSK_KEX2_1	558	560	PF00082	0.467
CLV_PCSK_KEX2_1	642	644	PF00082	0.495
CLV_PCSK_PC1ET2_1	334	336	PF00082	0.474
CLV_PCSK_PC1ET2_1	558	560	PF00082	0.467
CLV_PCSK_SKI1_1	168	172	PF00082	0.424
CLV_PCSK_SKI1_1	21	25	PF00082	0.556
CLV_PCSK_SKI1_1	411	415	PF00082	0.420
CLV_PCSK_SKI1_1	582	586	PF00082	0.511
CLV_PCSK_SKI1_1	67	71	PF00082	0.456
CLV_Separin_Metazoa	399	403	PF03568	0.384
DEG_APCC_DBOX_1	572	580	PF00400	0.300
DEG_MDM2_SWIB_1	322	329	PF02201	0.512
DOC_CYCLIN_RxL_1	579	589	PF00134	0.501
DOC_CYCLIN_yCln2_LP_2	344	350	PF00134	0.356
DOC_MAPK_DCC_7	232	242	PF00069	0.507
DOC_MAPK_gen_1	21	31	PF00069	0.428
DOC_MAPK_gen_1	337	346	PF00069	0.398
DOC_MAPK_MEF2A_6	232	241	PF00069	0.475
DOC_MAPK_MEF2A_6	573	581	PF00069	0.287
DOC_MAPK_MEF2A_6	762	769	PF00069	0.495
DOC_PP1_RVXF_1	19	26	PF00149	0.548
DOC_PP1_RVXF_1	598	605	PF00149	0.503
DOC_PP1_RVXF_1	621	627	PF00149	0.513
DOC_PP4_FxxP_1	4	7	PF00568	0.535
DOC_PP4_FxxP_1	568	571	PF00568	0.534
DOC_USP7_MATH_1	12	16	PF00917	0.516
DOC_USP7_MATH_1	190	194	PF00917	0.785
DOC_USP7_MATH_1	355	359	PF00917	0.467
DOC_USP7_MATH_1	415	419	PF00917	0.485
DOC_USP7_MATH_1	424	428	PF00917	0.592
DOC_USP7_MATH_1	57	61	PF00917	0.428
DOC_USP7_UBL2_3	380	384	PF12436	0.572
DOC_USP7_UBL2_3	554	558	PF12436	0.442
DOC_WW_Pin1_4	186	191	PF00397	0.773
DOC_WW_Pin1_4	194	199	PF00397	0.598
DOC_WW_Pin1_4	203	208	PF00397	0.507
DOC_WW_Pin1_4	418	423	PF00397	0.534
DOC_WW_Pin1_4	440	445	PF00397	0.450
DOC_WW_Pin1_4	92	97	PF00397	0.425
LIG_14-3-3_CanoR_1	129	133	PF00244	0.391
LIG_14-3-3_CanoR_1	146	156	PF00244	0.611
LIG_14-3-3_CanoR_1	324	330	PF00244	0.467
LIG_14-3-3_CanoR_1	337	347	PF00244	0.523
LIG_14-3-3_CanoR_1	354	360	PF00244	0.493
LIG_14-3-3_CanoR_1	365	370	PF00244	0.589
LIG_14-3-3_CanoR_1	573	577	PF00244	0.432
LIG_14-3-3_CanoR_1	600	605	PF00244	0.521
LIG_14-3-3_CanoR_1	629	634	PF00244	0.544
LIG_14-3-3_CanoR_1	67	72	PF00244	0.461
LIG_14-3-3_CanoR_1	75	82	PF00244	0.416
LIG_Actin_WH2_2	386	404	PF00022	0.518
LIG_BIR_II_1	1	5	PF00653	0.804
LIG_BRCT_BRCA1_1	342	346	PF00533	0.584
LIG_deltaCOP1_diTrp_1	594	604	PF00928	0.469
LIG_FHA_1	11	17	PF00498	0.690
LIG_FHA_1	161	167	PF00498	0.593
LIG_FHA_1	380	386	PF00498	0.527
LIG_FHA_1	458	464	PF00498	0.554
LIG_FHA_1	49	55	PF00498	0.429
LIG_FHA_1	512	518	PF00498	0.526
LIG_FHA_1	587	593	PF00498	0.336
LIG_FHA_1	626	632	PF00498	0.494
LIG_FHA_1	66	72	PF00498	0.435
LIG_FHA_1	750	756	PF00498	0.410
LIG_FHA_1	779	785	PF00498	0.559
LIG_FHA_1	88	94	PF00498	0.431
LIG_FHA_2	326	332	PF00498	0.486
LIG_FHA_2	68	74	PF00498	0.377
LIG_LIR_Apic_2	2	7	PF02991	0.535
LIG_LIR_Gen_1	218	226	PF02991	0.449
LIG_LIR_Nem_3	171	176	PF02991	0.433
LIG_LIR_Nem_3	213	219	PF02991	0.517
LIG_LIR_Nem_3	278	283	PF02991	0.510
LIG_LIR_Nem_3	352	356	PF02991	0.379
LIG_LIR_Nem_3	516	522	PF02991	0.371
LIG_LYPXL_yS_3	314	317	PF13949	0.458
LIG_NRBOX	396	402	PF00104	0.448
LIG_PDZ_Class_2	782	787	PF00595	0.516
LIG_Pex14_1	124	128	PF04695	0.391
LIG_Pex14_1	136	140	PF04695	0.422
LIG_Pex14_2	322	326	PF04695	0.491
LIG_PTB_Apo_2	779	786	PF02174	0.515
LIG_SH2_CRK	173	177	PF00017	0.475
LIG_SH2_CRK	353	357	PF00017	0.400
LIG_SH2_CRK	501	505	PF00017	0.494
LIG_SH2_GRB2like	780	783	PF00017	0.525
LIG_SH2_SRC	140	143	PF00017	0.615
LIG_SH2_STAP1	780	784	PF00017	0.524
LIG_SH2_STAT5	140	143	PF00017	0.458
LIG_SH2_STAT5	254	257	PF00017	0.449
LIG_SH2_STAT5	508	511	PF00017	0.394
LIG_SH2_STAT5	651	654	PF00017	0.392
LIG_SH2_STAT5	780	783	PF00017	0.525
LIG_SH2_STAT5	86	89	PF00017	0.421
LIG_SH3_1	232	238	PF00018	0.524
LIG_SH3_3	232	238	PF00018	0.524
LIG_SH3_3	260	266	PF00018	0.579
LIG_SH3_3	293	299	PF00018	0.538
LIG_SH3_3	4	10	PF00018	0.526
LIG_SH3_3	740	746	PF00018	0.550
LIG_SUMO_SIM_anti_2	208	214	PF11976	0.412
LIG_SUMO_SIM_anti_2	220	226	PF11976	0.384
LIG_SUMO_SIM_anti_2	68	74	PF11976	0.428
LIG_SUMO_SIM_par_1	208	214	PF11976	0.358
LIG_SUMO_SIM_par_1	238	245	PF11976	0.477
LIG_SUMO_SIM_par_1	50	56	PF11976	0.428
LIG_SUMO_SIM_par_1	67	74	PF11976	0.187
LIG_SUMO_SIM_par_1	95	100	PF11976	0.228
LIG_TRAF2_1	116	119	PF00917	0.428
LIG_TRAF2_1	330	333	PF00917	0.587
LIG_TRAF2_1	369	372	PF00917	0.440
LIG_TRAF2_1	396	399	PF00917	0.320
LIG_TRAF2_1	478	481	PF00917	0.579
LIG_TRAF2_1	685	688	PF00917	0.509
LIG_TRAF2_1	725	728	PF00917	0.489
LIG_TRAF2_1	756	759	PF00917	0.348
LIG_TYR_ITIM	312	317	PF00017	0.363
LIG_TYR_ITIM	517	522	PF00017	0.498
LIG_UBA3_1	344	351	PF00899	0.499
MOD_CK1_1	183	189	PF00069	0.644
MOD_CK1_1	205	211	PF00069	0.539
MOD_CK1_1	220	226	PF00069	0.504
MOD_CK1_1	340	346	PF00069	0.603
MOD_CK1_1	418	424	PF00069	0.549
MOD_CK1_1	572	578	PF00069	0.545
MOD_CK1_1	603	609	PF00069	0.517
MOD_CK1_1	683	689	PF00069	0.573
MOD_CK2_1	325	331	PF00069	0.526
MOD_CK2_1	365	371	PF00069	0.536
MOD_Cter_Amidation	143	146	PF01082	0.445
MOD_GlcNHglycan	1	4	PF01048	0.782
MOD_GlcNHglycan	142	145	PF01048	0.619
MOD_GlcNHglycan	149	152	PF01048	0.608
MOD_GlcNHglycan	163	166	PF01048	0.536
MOD_GlcNHglycan	192	195	PF01048	0.734
MOD_GlcNHglycan	244	247	PF01048	0.483
MOD_GlcNHglycan	348	351	PF01048	0.347
MOD_GlcNHglycan	367	370	PF01048	0.459
MOD_GlcNHglycan	428	431	PF01048	0.463
MOD_GlcNHglycan	505	508	PF01048	0.497
MOD_GlcNHglycan	605	608	PF01048	0.557
MOD_GlcNHglycan	668	672	PF01048	0.591
MOD_GSK3_1	12	19	PF00069	0.674
MOD_GSK3_1	157	164	PF00069	0.664
MOD_GSK3_1	175	182	PF00069	0.619
MOD_GSK3_1	186	193	PF00069	0.794
MOD_GSK3_1	333	340	PF00069	0.615
MOD_GSK3_1	48	55	PF00069	0.359
MOD_GSK3_1	625	632	PF00069	0.542
MOD_GSK3_1	711	718	PF00069	0.553
MOD_GSK3_1	744	751	PF00069	0.521
MOD_GSK3_1	88	95	PF00069	0.423
MOD_N-GLC_1	180	185	PF02516	0.602
MOD_NEK2_1	179	184	PF00069	0.746
MOD_NEK2_1	225	230	PF00069	0.462
MOD_NEK2_1	241	246	PF00069	0.482
MOD_NEK2_1	250	255	PF00069	0.483
MOD_NEK2_1	425	430	PF00069	0.499
MOD_NEK2_1	457	462	PF00069	0.396
MOD_NEK2_1	48	53	PF00069	0.321
MOD_NEK2_1	512	517	PF00069	0.469
MOD_NEK2_1	586	591	PF00069	0.433
MOD_NEK2_1	669	674	PF00069	0.531
MOD_NEK2_1	705	710	PF00069	0.704
MOD_NEK2_1	87	92	PF00069	0.440
MOD_NEK2_1	97	102	PF00069	0.445
MOD_NEK2_2	12	17	PF00069	0.516
MOD_PIKK_1	264	270	PF00454	0.612
MOD_PIKK_1	680	686	PF00454	0.546
MOD_PIKK_1	715	721	PF00454	0.430
MOD_PKA_1	365	371	PF00069	0.419
MOD_PKA_2	128	134	PF00069	0.353
MOD_PKA_2	16	22	PF00069	0.437
MOD_PKA_2	242	248	PF00069	0.469
MOD_PKA_2	338	344	PF00069	0.529
MOD_PKA_2	365	371	PF00069	0.429
MOD_PKA_2	572	578	PF00069	0.432
MOD_PKB_1	335	343	PF00069	0.585
MOD_Plk_1	217	223	PF00069	0.529
MOD_Plk_1	705	711	PF00069	0.638
MOD_Plk_4	205	211	PF00069	0.391
MOD_Plk_4	220	226	PF00069	0.478
MOD_Plk_4	340	346	PF00069	0.593
MOD_Plk_4	355	361	PF00069	0.510
MOD_Plk_4	513	519	PF00069	0.506
MOD_Plk_4	57	63	PF00069	0.362
MOD_Plk_4	572	578	PF00069	0.479
MOD_Plk_4	67	73	PF00069	0.308
MOD_Plk_4	97	103	PF00069	0.484
MOD_ProDKin_1	186	192	PF00069	0.776
MOD_ProDKin_1	194	200	PF00069	0.595
MOD_ProDKin_1	203	209	PF00069	0.497
MOD_ProDKin_1	418	424	PF00069	0.529
MOD_ProDKin_1	440	446	PF00069	0.445
MOD_ProDKin_1	92	98	PF00069	0.425
MOD_SUMO_rev_2	331	336	PF00179	0.579
TRG_DiLeu_BaEn_1	677	682	PF01217	0.517
TRG_DiLeu_BaEn_1	759	764	PF01217	0.343
TRG_DiLeu_BaLyEn_6	580	585	PF01217	0.501
TRG_ENDOCYTIC_2	173	176	PF00928	0.525
TRG_ENDOCYTIC_2	216	219	PF00928	0.528
TRG_ENDOCYTIC_2	314	317	PF00928	0.344
TRG_ENDOCYTIC_2	353	356	PF00928	0.367
TRG_ENDOCYTIC_2	519	522	PF00928	0.380
TRG_ER_diArg_1	145	147	PF00400	0.495
TRG_ER_diArg_1	322	324	PF00400	0.456
TRG_ER_diArg_1	364	366	PF00400	0.436
TRG_ER_diArg_1	616	619	PF00400	0.517
TRG_ER_diArg_1	765	768	PF00400	0.353
TRG_Pf-PMV_PEXEL_1	520	524	PF00026	0.443

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5J2	Leptomonas seymouri	50%	100%
A0A0S4JRQ2	Bodo saltans	24%	96%
A0A1X0NR91	Trypanosomatidae	26%	100%
A0A3R7MGL1	Trypanosoma rangeli	27%	100%
A0A3S7X724	Leishmania donovani	85%	98%
A4HLX6	Leishmania braziliensis	72%	100%
A4I9A5	Leishmania infantum	85%	98%
C9ZJC1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
E9B4A5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
V5AV29	Trypanosoma cruzi	27%	100%