Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme, putative

Species:

Leishmania major

UniProt:

Q4Q3Q8_LEIMA

TriTrypDb:

LmjF.33.2770 , LMJLV39_330038900 , LMJSD75_330038400

Length:

243

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q3Q8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3Q8

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016740	transferase activity	2	10
GO:0016874	ligase activity	2	5
GO:0017076	purine nucleotide binding	4	11
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0061631	ubiquitin conjugating enzyme activity	5	3
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	3
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	3
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	139	141	PF00675	0.249
CLV_NRD_NRD_1	241	243	PF00675	0.582
CLV_PCSK_KEX2_1	241	243	PF00082	0.561
CLV_PCSK_KEX2_1	94	96	PF00082	0.268
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.268
CLV_PCSK_SKI1_1	104	108	PF00082	0.291
CLV_PCSK_SKI1_1	111	115	PF00082	0.256
CLV_PCSK_SKI1_1	129	133	PF00082	0.249
CLV_PCSK_SKI1_1	212	216	PF00082	0.590
CLV_PCSK_SKI1_1	237	241	PF00082	0.543
CLV_PCSK_SKI1_1	70	74	PF00082	0.249
DEG_APCC_DBOX_1	103	111	PF00400	0.293
DEG_APCC_DBOX_1	157	165	PF00400	0.494
DEG_SCF_TRCP1_1	184	190	PF00400	0.475
DOC_CYCLIN_RxL_1	105	116	PF00134	0.249
DOC_CYCLIN_RxL_1	209	218	PF00134	0.575
DOC_MAPK_MEF2A_6	53	60	PF00069	0.381
DOC_MAPK_MEF2A_6	94	103	PF00069	0.270
DOC_PP4_FxxP_1	159	162	PF00568	0.512
DOC_SPAK_OSR1_1	53	57	PF12202	0.381
DOC_USP7_MATH_1	169	173	PF00917	0.641
DOC_USP7_MATH_1	175	179	PF00917	0.668
DOC_USP7_MATH_1	182	186	PF00917	0.679
DOC_USP7_MATH_1	194	198	PF00917	0.718
DOC_USP7_MATH_1	232	236	PF00917	0.604
DOC_USP7_MATH_1	30	34	PF00917	0.328
DOC_USP7_UBL2_3	234	238	PF12436	0.506
DOC_WW_Pin1_4	44	49	PF00397	0.249
DOC_WW_Pin1_4	95	100	PF00397	0.323
LIG_14-3-3_CanoR_1	158	162	PF00244	0.687
LIG_14-3-3_CanoR_1	212	217	PF00244	0.565
LIG_Actin_RPEL_3	88	107	PF02755	0.293
LIG_APCC_ABBA_1	57	62	PF00400	0.235
LIG_EVH1_2	68	72	PF00568	0.381
LIG_FHA_1	209	215	PF00498	0.574
LIG_FHA_2	158	164	PF00498	0.563
LIG_FHA_2	213	219	PF00498	0.666
LIG_FHA_2	225	231	PF00498	0.671
LIG_Integrin_RGD_1	85	87	PF01839	0.381
LIG_LIR_Nem_3	133	138	PF02991	0.275
LIG_LIR_Nem_3	61	67	PF02991	0.271
LIG_LYPXL_yS_3	64	67	PF13949	0.249
LIG_REV1ctd_RIR_1	70	79	PF16727	0.381
LIG_SH2_CRK	145	149	PF00017	0.424
LIG_SH2_NCK_1	145	149	PF00017	0.424
LIG_SH2_STAP1	138	142	PF00017	0.381
LIG_SH2_STAT5	145	148	PF00017	0.424
LIG_SH3_2	65	70	PF14604	0.249
LIG_SH3_3	38	44	PF00018	0.180
LIG_SH3_3	62	68	PF00018	0.289
LIG_SUMO_SIM_par_1	55	62	PF11976	0.349
MOD_CK1_1	185	191	PF00069	0.655
MOD_CK1_1	197	203	PF00069	0.649
MOD_CK1_1	208	214	PF00069	0.647
MOD_CK2_1	157	163	PF00069	0.557
MOD_CK2_1	212	218	PF00069	0.646
MOD_CK2_1	224	230	PF00069	0.698
MOD_CK2_1	30	36	PF00069	0.447
MOD_GlcNHglycan	120	123	PF01048	0.283
MOD_GlcNHglycan	169	172	PF01048	0.589
MOD_GlcNHglycan	177	180	PF01048	0.596
MOD_GlcNHglycan	184	187	PF01048	0.708
MOD_GlcNHglycan	196	199	PF01048	0.550
MOD_GlcNHglycan	224	227	PF01048	0.577
MOD_GSK3_1	167	174	PF00069	0.619
MOD_GSK3_1	208	215	PF00069	0.628
MOD_N-GLC_1	80	85	PF02516	0.359
MOD_NEK2_1	72	77	PF00069	0.342
MOD_NEK2_2	89	94	PF00069	0.168
MOD_PKA_2	157	163	PF00069	0.601
MOD_PKA_2	28	34	PF00069	0.349
MOD_ProDKin_1	44	50	PF00069	0.249
MOD_ProDKin_1	95	101	PF00069	0.323
MOD_SUMO_for_1	164	167	PF00179	0.473
MOD_SUMO_rev_2	133	143	PF00179	0.381
TRG_DiLeu_BaEn_1	126	131	PF01217	0.249
TRG_DiLeu_LyEn_5	126	131	PF01217	0.249
TRG_ENDOCYTIC_2	145	148	PF00928	0.517
TRG_ENDOCYTIC_2	64	67	PF00928	0.272
TRG_ER_diArg_1	240	242	PF00400	0.552
TRG_NLS_MonoExtN_4	234	240	PF00514	0.648
TRG_Pf-PMV_PEXEL_1	111	116	PF00026	0.249
TRG_Pf-PMV_PEXEL_1	129	133	PF00026	0.249
TRG_Pf-PMV_PEXEL_1	9	14	PF00026	0.310

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P683	Leptomonas seymouri	71%	100%
A0A0N1I1I8	Leptomonas seymouri	30%	100%
A0A1X0NSR5	Trypanosomatidae	58%	87%
A0A3Q8IMQ9	Leishmania donovani	88%	100%
A0A3S5H604	Leishmania donovani	41%	100%
A0A422NKC5	Trypanosoma rangeli	51%	100%
A4H533	Leishmania braziliensis	34%	100%
A4H861	Leishmania braziliensis	79%	100%
A4HTB1	Leishmania infantum	41%	100%
A4I9B7	Leishmania infantum	88%	100%
B5DFI8	Rattus norvegicus	39%	100%
C6K3V1	Leptomonas seymouri	26%	80%
C9ZJA6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	98%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B4B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
E9B4R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	83%
P49427	Homo sapiens	32%	100%
Q16763	Homo sapiens	38%	100%
Q29503	Oryctolagus cuniculus	31%	100%
Q32LD2	Bos taurus	30%	100%
Q4Q3B5	Leishmania major	28%	84%
Q4QIK2	Leishmania major	41%	100%
Q6ZWZ2	Mus musculus	31%	100%
Q712K3	Homo sapiens	31%	100%
Q8CFI2	Mus musculus	32%	100%
Q9FF66	Arabidopsis thaliana	43%	97%
V5BAJ3	Trypanosoma cruzi	48%	100%