EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4Q3L4_LEIMA

TriTrypDb:

LmjF.33.3220 , LMJLV39_330045500 , LMJSD75_330044300

Length:

534

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0005813	centrosome	3	2
GO:0005815	microtubule organizing center	2	2
GO:0110165	cellular anatomical entity	1	12
GO:0016020	membrane	2	2

Expansion

Sequence features

Q4Q3L4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3L4

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0019220	regulation of phosphate metabolic process	6	12
GO:0019222	regulation of metabolic process	3	12
GO:0030865	cortical cytoskeleton organization	6	2
GO:0031323	regulation of cellular metabolic process	4	12
GO:0035303	regulation of dephosphorylation	7	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0051174	regulation of phosphorus metabolic process	5	12
GO:0065007	biological regulation	1	12
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005509	calcium ion binding	5	7
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	258	262	PF00656	0.421
CLV_C14_Caspase3-7	359	363	PF00656	0.488
CLV_NRD_NRD_1	102	104	PF00675	0.410
CLV_NRD_NRD_1	21	23	PF00675	0.470
CLV_NRD_NRD_1	222	224	PF00675	0.344
CLV_PCSK_KEX2_1	102	104	PF00082	0.468
CLV_PCSK_KEX2_1	139	141	PF00082	0.399
CLV_PCSK_PC1ET2_1	139	141	PF00082	0.399
CLV_PCSK_SKI1_1	13	17	PF00082	0.578
CLV_PCSK_SKI1_1	139	143	PF00082	0.390
CLV_PCSK_SKI1_1	224	228	PF00082	0.352
CLV_PCSK_SKI1_1	442	446	PF00082	0.459
CLV_Separin_Metazoa	250	254	PF03568	0.384
DEG_APCC_DBOX_1	139	147	PF00400	0.385
DOC_MAPK_gen_1	145	152	PF00069	0.369
DOC_MAPK_gen_1	99	107	PF00069	0.420
DOC_MAPK_MEF2A_6	145	152	PF00069	0.336
DOC_PP1_RVXF_1	101	108	PF00149	0.421
DOC_PP1_RVXF_1	138	145	PF00149	0.471
DOC_PP4_FxxP_1	107	110	PF00568	0.415
DOC_PP4_FxxP_1	111	114	PF00568	0.356
DOC_PP4_FxxP_1	278	281	PF00568	0.592
DOC_USP7_MATH_1	121	125	PF00917	0.571
DOC_USP7_MATH_1	177	181	PF00917	0.517
DOC_USP7_MATH_1	349	353	PF00917	0.505
DOC_USP7_MATH_2	336	342	PF00917	0.423
DOC_USP7_UBL2_3	438	442	PF12436	0.544
DOC_WW_Pin1_4	110	115	PF00397	0.381
DOC_WW_Pin1_4	178	183	PF00397	0.495
DOC_WW_Pin1_4	277	282	PF00397	0.567
DOC_WW_Pin1_4	29	34	PF00397	0.355
DOC_WW_Pin1_4	84	89	PF00397	0.447
LIG_14-3-3_CanoR_1	102	106	PF00244	0.453
LIG_14-3-3_CanoR_1	122	129	PF00244	0.233
LIG_Actin_WH2_2	189	206	PF00022	0.379
LIG_APCC_ABBAyCdc20_2	244	250	PF00400	0.383
LIG_BIR_III_4	362	366	PF00653	0.547
LIG_BRCT_BRCA1_1	103	107	PF00533	0.463
LIG_BRCT_BRCA1_1	221	225	PF00533	0.341
LIG_BRCT_BRCA1_1	244	248	PF00533	0.382
LIG_Clathr_ClatBox_1	339	343	PF01394	0.613
LIG_Clathr_ClatBox_1	431	435	PF01394	0.451
LIG_deltaCOP1_diTrp_1	100	107	PF00928	0.525
LIG_deltaCOP1_diTrp_1	485	489	PF00928	0.477
LIG_eIF4E_1	334	340	PF01652	0.601
LIG_EVH1_1	173	177	PF00568	0.599
LIG_EVH1_2	157	161	PF00568	0.551
LIG_FHA_1	229	235	PF00498	0.418
LIG_FHA_1	398	404	PF00498	0.574
LIG_FHA_2	186	192	PF00498	0.383
LIG_FHA_2	357	363	PF00498	0.393
LIG_FHA_2	368	374	PF00498	0.402
LIG_FHA_2	486	492	PF00498	0.472
LIG_FHA_2	62	68	PF00498	0.573
LIG_FHA_2	85	91	PF00498	0.486
LIG_LIR_Apic_2	104	110	PF02991	0.443
LIG_LIR_Gen_1	116	121	PF02991	0.417
LIG_LIR_Gen_1	222	230	PF02991	0.369
LIG_LIR_Gen_1	245	255	PF02991	0.380
LIG_LIR_Gen_1	352	363	PF02991	0.407
LIG_LIR_Gen_1	380	390	PF02991	0.390
LIG_LIR_Gen_1	408	415	PF02991	0.363
LIG_LIR_Gen_1	42	52	PF02991	0.449
LIG_LIR_Gen_1	485	492	PF02991	0.407
LIG_LIR_Gen_1	526	533	PF02991	0.679
LIG_LIR_Gen_1	63	72	PF02991	0.456
LIG_LIR_Nem_3	116	120	PF02991	0.407
LIG_LIR_Nem_3	245	251	PF02991	0.372
LIG_LIR_Nem_3	323	329	PF02991	0.301
LIG_LIR_Nem_3	343	348	PF02991	0.343
LIG_LIR_Nem_3	352	358	PF02991	0.383
LIG_LIR_Nem_3	380	385	PF02991	0.388
LIG_LIR_Nem_3	405	409	PF02991	0.372
LIG_LIR_Nem_3	42	47	PF02991	0.418
LIG_LIR_Nem_3	485	489	PF02991	0.408
LIG_LIR_Nem_3	507	511	PF02991	0.363
LIG_LIR_Nem_3	526	531	PF02991	0.521
LIG_LIR_Nem_3	63	68	PF02991	0.484
LIG_LIR_Nem_3	71	75	PF02991	0.369
LIG_MLH1_MIPbox_1	103	107	PF16413	0.486
LIG_MYND_1	110	114	PF01753	0.372
LIG_PCNA_PIPBox_1	351	360	PF02747	0.386
LIG_Pex14_1	212	216	PF04695	0.397
LIG_Pex14_2	107	111	PF04695	0.406
LIG_PTB_Apo_2	376	383	PF02174	0.484
LIG_PTB_Apo_2	74	81	PF02174	0.347
LIG_PTB_Phospho_1	74	80	PF10480	0.372
LIG_SH2_CRK	50	54	PF00017	0.491
LIG_SH2_CRK	80	84	PF00017	0.397
LIG_SH2_GRB2like	26	29	PF00017	0.531
LIG_SH2_GRB2like	377	380	PF00017	0.505
LIG_SH2_GRB2like	409	412	PF00017	0.467
LIG_SH2_NCK_1	528	532	PF00017	0.628
LIG_SH2_SRC	26	29	PF00017	0.587
LIG_SH2_SRC	334	337	PF00017	0.515
LIG_SH2_SRC	528	531	PF00017	0.627
LIG_SH2_STAP1	134	138	PF00017	0.382
LIG_SH2_STAP1	65	69	PF00017	0.533
LIG_SH2_STAP1	80	84	PF00017	0.292
LIG_SH2_STAT3	307	310	PF00017	0.498
LIG_SH2_STAT5	106	109	PF00017	0.394
LIG_SH2_STAT5	217	220	PF00017	0.344
LIG_SH2_STAT5	26	29	PF00017	0.353
LIG_SH2_STAT5	334	337	PF00017	0.566
LIG_SH2_STAT5	409	412	PF00017	0.361
LIG_SH2_STAT5	427	430	PF00017	0.282
LIG_SH2_STAT5	6	9	PF00017	0.582
LIG_SH3_3	126	132	PF00018	0.469
LIG_SH3_3	171	177	PF00018	0.582
LIG_SH3_3	269	275	PF00018	0.450
LIG_SUMO_SIM_anti_2	347	352	PF11976	0.403
LIG_SUMO_SIM_par_1	430	435	PF11976	0.369
LIG_SUMO_SIM_par_1	462	467	PF11976	0.529
LIG_SUMO_SIM_par_1	502	507	PF11976	0.360
LIG_TRAF2_1	15	18	PF00917	0.604
LIG_TRAF2_1	370	373	PF00917	0.540
LIG_TYR_ITIM	48	53	PF00017	0.557
LIG_UBA3_1	431	438	PF00899	0.400
MOD_CK1_1	113	119	PF00069	0.412
MOD_CK1_1	178	184	PF00069	0.490
MOD_CK1_1	328	334	PF00069	0.481
MOD_CK1_1	375	381	PF00069	0.417
MOD_CK1_1	467	473	PF00069	0.380
MOD_CK2_1	11	17	PF00069	0.563
MOD_CK2_1	367	373	PF00069	0.411
MOD_CK2_1	84	90	PF00069	0.432
MOD_CMANNOS	483	486	PF00535	0.438
MOD_GlcNHglycan	13	16	PF01048	0.527
MOD_GlcNHglycan	153	157	PF01048	0.508
MOD_GlcNHglycan	177	180	PF01048	0.510
MOD_GlcNHglycan	397	400	PF01048	0.499
MOD_GlcNHglycan	465	469	PF01048	0.404
MOD_GlcNHglycan	493	497	PF01048	0.426
MOD_GlcNHglycan	522	525	PF01048	0.588
MOD_GlcNHglycan	8	11	PF01048	0.533
MOD_GSK3_1	177	184	PF00069	0.491
MOD_GSK3_1	242	249	PF00069	0.414
MOD_GSK3_1	35	42	PF00069	0.504
MOD_GSK3_1	393	400	PF00069	0.483
MOD_GSK3_1	4	11	PF00069	0.576
MOD_LATS_1	440	446	PF00433	0.440
MOD_N-GLC_1	378	383	PF02516	0.482
MOD_NEK2_1	120	125	PF00069	0.520
MOD_NEK2_1	152	157	PF00069	0.467
MOD_NEK2_1	228	233	PF00069	0.442
MOD_NEK2_1	34	39	PF00069	0.493
MOD_NEK2_1	4	9	PF00069	0.629
MOD_NEK2_1	434	439	PF00069	0.445
MOD_NEK2_2	101	106	PF00069	0.484
MOD_PIKK_1	121	127	PF00454	0.462
MOD_PIKK_1	185	191	PF00454	0.407
MOD_PIKK_1	380	386	PF00454	0.494
MOD_PIKK_1	8	14	PF00454	0.607
MOD_PKA_2	101	107	PF00069	0.425
MOD_PKA_2	121	127	PF00069	0.249
MOD_Plk_1	285	291	PF00069	0.427
MOD_Plk_1	372	378	PF00069	0.425
MOD_Plk_2-3	338	344	PF00069	0.512
MOD_Plk_2-3	485	491	PF00069	0.516
MOD_Plk_4	113	119	PF00069	0.413
MOD_Plk_4	39	45	PF00069	0.476
MOD_Plk_4	405	411	PF00069	0.382
MOD_Plk_4	467	473	PF00069	0.394
MOD_Plk_4	497	503	PF00069	0.394
MOD_ProDKin_1	110	116	PF00069	0.378
MOD_ProDKin_1	178	184	PF00069	0.488
MOD_ProDKin_1	277	283	PF00069	0.562
MOD_ProDKin_1	29	35	PF00069	0.354
MOD_ProDKin_1	84	90	PF00069	0.451
MOD_SUMO_for_1	243	246	PF00179	0.512
MOD_SUMO_rev_2	313	318	PF00179	0.554
TRG_AP2beta_CARGO_1	380	389	PF09066	0.392
TRG_DiLeu_BaLyEn_6	137	142	PF01217	0.397
TRG_ENDOCYTIC_2	326	329	PF00928	0.440
TRG_ENDOCYTIC_2	355	358	PF00928	0.383
TRG_ENDOCYTIC_2	409	412	PF00928	0.363
TRG_ENDOCYTIC_2	50	53	PF00928	0.563
TRG_ENDOCYTIC_2	508	511	PF00928	0.382
TRG_ENDOCYTIC_2	528	531	PF00928	0.375
TRG_ENDOCYTIC_2	65	68	PF00928	0.297
TRG_ENDOCYTIC_2	75	78	PF00928	0.346
TRG_ENDOCYTIC_2	80	83	PF00928	0.357
TRG_ER_diArg_1	101	103	PF00400	0.496
TRG_ER_diArg_1	144	147	PF00400	0.378
TRG_Pf-PMV_PEXEL_1	401	405	PF00026	0.464

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKT4	Leptomonas seymouri	82%	99%
A0A0S4J574	Bodo saltans	49%	93%
A0A1X0NRG1	Trypanosomatidae	64%	100%
A0A3R7REK3	Trypanosoma rangeli	64%	100%
A0A3S7X7C5	Leishmania donovani	96%	100%
A4HM30	Leishmania braziliensis	91%	100%
A4I9G3	Leishmania infantum	96%	100%
C9ZJT8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	87%
E9B4G1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
V5BJB1	Trypanosoma cruzi	62%	93%