Ascorbate peroxidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ascorbate peroxidase

Species:

Leishmania major

UniProt:

Q4Q3K2_LEIMA

TriTrypDb:

LmjF.34.0070 , LMJLV39_340005700 , LMJSD75_340005700

Length:

303

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4Q3K2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3K2

PDB structure(s): 3riv_A , 3riv_B , 3riw_A , 3riw_B , 4ged_A , 5al9_A , 5ala_A , 5ala_B , 5amm_A , 5amm_B

Function

Biological processes
Term	Name	Level	Count
GO:0000302	response to reactive oxygen species	4	2
GO:0006950	response to stress	2	10
GO:0006979	response to oxidative stress	3	10
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009987	cellular process	1	10
GO:0010941	regulation of cell death	4	2
GO:0030322	stabilization of membrane potential	4	2
GO:0033554	cellular response to stress	3	10
GO:0034599	cellular response to oxidative stress	4	10
GO:0042221	response to chemical	2	10
GO:0042391	regulation of membrane potential	3	2
GO:0042592	homeostatic process	3	2
GO:0042743	hydrogen peroxide metabolic process	4	2
GO:0042744	hydrogen peroxide catabolic process	4	2
GO:0042981	regulation of apoptotic process	6	2
GO:0043066	negative regulation of apoptotic process	7	2
GO:0043067	regulation of programmed cell death	5	2
GO:0043069	negative regulation of programmed cell death	6	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0048519	negative regulation of biological process	3	2
GO:0048523	negative regulation of cellular process	4	2
GO:0048878	chemical homeostasis	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050801	monoatomic ion homeostasis	5	2
GO:0050896	response to stimulus	1	10
GO:0051716	cellular response to stimulus	2	10
GO:0055065	obsolete metal ion homeostasis	7	2
GO:0055074	calcium ion homeostasis	8	2
GO:0055080	monoatomic cation homeostasis	6	2
GO:0060548	negative regulation of cell death	5	2
GO:0062197	cellular response to chemical stress	4	10
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0070887	cellular response to chemical stimulus	3	10
GO:0072507	obsolete divalent inorganic cation homeostasis	7	2
GO:0072593	reactive oxygen species metabolic process	3	2
GO:0098771	inorganic ion homeostasis	6	2
GO:1901700	response to oxygen-containing compound	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004129	cytochrome-c oxidase activity	4	2
GO:0004447	iodide peroxidase activity	4	2
GO:0004601	peroxidase activity	2	10
GO:0005215	transporter activity	1	2
GO:0005488	binding	1	10
GO:0008324	monoatomic cation transmembrane transporter activity	4	2
GO:0008447	L-ascorbate oxidase activity	5	2
GO:0009055	electron transfer activity	3	2
GO:0015075	monoatomic ion transmembrane transporter activity	3	2
GO:0015078	proton transmembrane transporter activity	5	2
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	2
GO:0015399	primary active transmembrane transporter activity	4	2
GO:0015453	oxidoreduction-driven active transmembrane transporter activity	5	2
GO:0016209	antioxidant activity	1	10
GO:0016491	oxidoreductase activity	2	10
GO:0016675	oxidoreductase activity, acting on a heme group of donors	3	2
GO:0016679	oxidoreductase activity, acting on diphenols and related substances as donors	3	2
GO:0016682	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor	4	2
GO:0016684	oxidoreductase activity, acting on peroxide as acceptor	3	10
GO:0016688	L-ascorbate peroxidase activity	3	6
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	2
GO:0020037	heme binding	4	10
GO:0022804	active transmembrane transporter activity	3	2
GO:0022853	active monoatomic ion transmembrane transporter activity	4	2
GO:0022857	transmembrane transporter activity	2	2
GO:0022890	inorganic cation transmembrane transporter activity	4	2
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046906	tetrapyrrole binding	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:0140905	haloperoxidase activity	3	2
GO:0140906	halogenase activity	4	2
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	148	152	PF00656	0.302
CLV_NRD_NRD_1	104	106	PF00675	0.542
CLV_NRD_NRD_1	176	178	PF00675	0.556
CLV_NRD_NRD_1	262	264	PF00675	0.573
CLV_NRD_NRD_1	6	8	PF00675	0.556
CLV_PCSK_KEX2_1	176	178	PF00082	0.556
CLV_PCSK_KEX2_1	6	8	PF00082	0.556
CLV_PCSK_PC7_1	172	178	PF00082	0.436
CLV_PCSK_SKI1_1	105	109	PF00082	0.489
CLV_PCSK_SKI1_1	172	176	PF00082	0.520
CLV_PCSK_SKI1_1	285	289	PF00082	0.569
CLV_PCSK_SKI1_1	6	10	PF00082	0.554
DEG_APCC_DBOX_1	104	112	PF00400	0.286
DOC_CYCLIN_RxL_1	170	184	PF00134	0.236
DOC_CYCLIN_RxL_1	6	13	PF00134	0.548
DOC_PP1_RVXF_1	174	181	PF00149	0.321
DOC_PP1_RVXF_1	62	68	PF00149	0.302
DOC_PP2B_LxvP_1	101	104	PF13499	0.373
DOC_USP7_MATH_1	162	166	PF00917	0.328
DOC_WW_Pin1_4	78	83	PF00397	0.328
LIG_14-3-3_CanoR_1	242	250	PF00244	0.403
LIG_BIR_II_1	1	5	PF00653	0.692
LIG_BRCT_BRCA1_1	85	89	PF00533	0.302
LIG_FHA_1	183	189	PF00498	0.284
LIG_FHA_1	219	225	PF00498	0.373
LIG_FHA_1	242	248	PF00498	0.302
LIG_FHA_1	290	296	PF00498	0.395
LIG_FHA_2	191	197	PF00498	0.314
LIG_LIR_Apic_2	202	207	PF02991	0.302
LIG_LIR_Gen_1	119	128	PF02991	0.304
LIG_LIR_Gen_1	218	224	PF02991	0.302
LIG_LIR_Gen_1	72	79	PF02991	0.403
LIG_LIR_Nem_3	119	123	PF02991	0.304
LIG_LIR_Nem_3	173	178	PF02991	0.320
LIG_LIR_Nem_3	200	204	PF02991	0.309
LIG_LIR_Nem_3	218	223	PF02991	0.293
LIG_LIR_Nem_3	259	265	PF02991	0.288
LIG_LIR_Nem_3	72	76	PF02991	0.409
LIG_LYPXL_yS_3	115	118	PF13949	0.320
LIG_Pex14_2	215	219	PF04695	0.326
LIG_Pex14_2	276	280	PF04695	0.352
LIG_PTB_Apo_2	214	221	PF02174	0.302
LIG_SH2_PTP2	129	132	PF00017	0.373
LIG_SH2_PTP2	265	268	PF00017	0.403
LIG_SH2_STAP1	120	124	PF00017	0.302
LIG_SH2_STAT5	129	132	PF00017	0.373
LIG_SH2_STAT5	265	268	PF00017	0.361
LIG_SH3_3	135	141	PF00018	0.290
LIG_SH3_3	151	157	PF00018	0.282
LIG_SH3_3	226	232	PF00018	0.403
LIG_SH3_3	31	37	PF00018	0.430
LIG_SUMO_SIM_anti_2	184	190	PF11976	0.306
LIG_TYR_ITIM	118	123	PF00017	0.302
LIG_WRC_WIRS_1	17	22	PF05994	0.381
MOD_CK1_1	2	8	PF00069	0.767
MOD_CK1_1	218	224	PF00069	0.302
MOD_CK1_1	28	34	PF00069	0.629
MOD_CK1_1	81	87	PF00069	0.328
MOD_CK2_1	190	196	PF00069	0.314
MOD_GlcNHglycan	1	4	PF01048	0.546
MOD_GlcNHglycan	200	204	PF01048	0.519
MOD_GlcNHglycan	21	24	PF01048	0.498
MOD_GlcNHglycan	30	33	PF01048	0.613
MOD_GlcNHglycan	83	86	PF01048	0.520
MOD_GSK3_1	162	169	PF00069	0.413
MOD_GSK3_1	285	292	PF00069	0.396
MOD_NEK2_1	1	6	PF00069	0.729
MOD_NEK2_1	215	220	PF00069	0.302
MOD_NEK2_1	25	30	PF00069	0.446
MOD_NEK2_2	107	112	PF00069	0.312
MOD_PIKK_1	164	170	PF00454	0.312
MOD_PIKK_1	218	224	PF00454	0.403
MOD_PK_1	150	156	PF00069	0.302
MOD_PKA_1	285	291	PF00069	0.418
MOD_PKA_2	241	247	PF00069	0.343
MOD_Plk_1	215	221	PF00069	0.302
MOD_Plk_2-3	69	75	PF00069	0.320
MOD_Plk_4	16	22	PF00069	0.382
MOD_Plk_4	215	221	PF00069	0.302
MOD_Plk_4	58	64	PF00069	0.405
MOD_ProDKin_1	78	84	PF00069	0.328
MOD_SUMO_for_1	232	235	PF00179	0.363
MOD_SUMO_for_1	89	92	PF00179	0.343
TRG_DiLeu_BaEn_1	184	189	PF01217	0.302
TRG_DiLeu_BaEn_1	235	240	PF01217	0.348
TRG_ENDOCYTIC_2	115	118	PF00928	0.306
TRG_ENDOCYTIC_2	120	123	PF00928	0.297
TRG_ENDOCYTIC_2	129	132	PF00928	0.291
TRG_ENDOCYTIC_2	265	268	PF00928	0.310
TRG_ENDOCYTIC_2	73	76	PF00928	0.404
TRG_ER_diArg_1	175	177	PF00400	0.356
TRG_NES_CRM1_1	122	133	PF08389	0.308
TRG_NLS_MonoExtC_3	111	116	PF00514	0.320
TRG_Pf-PMV_PEXEL_1	285	289	PF00026	0.581
TRG_Pf-PMV_PEXEL_1	42	47	PF00026	0.574

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3Y9	Leptomonas seymouri	25%	74%
A0A0N0P6I0	Leptomonas seymouri	77%	93%
A0A0S4IHN4	Bodo saltans	56%	94%
A0A1X0P9U7	Trypanosomatidae	67%	92%
A0A3S7WWR4	Leishmania donovani	25%	89%
A2XFC7	Oryza sativa subsp. indica	42%	100%
A4HAD2	Leishmania braziliensis	82%	99%
A4HC12	Leishmania braziliensis	25%	78%
A4HZF3	Leishmania infantum	25%	89%
A4I9H5	Leishmania infantum	96%	100%
A4QVH4	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	45%	84%
A4R606	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	43%	100%
A5H454	Zea mays	23%	95%
E9AVE9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	89%
E9B4H3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
H6V7N3	Leishmania donovani	96%	100%
O23237	Arabidopsis thaliana	22%	92%
O23474	Arabidopsis thaliana	28%	87%
O48677	Arabidopsis thaliana	28%	93%
O80912	Arabidopsis thaliana	25%	87%
O81772	Arabidopsis thaliana	26%	93%
P00431	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	41%	84%
P00434	Brassica rapa subsp. rapa	24%	100%
P0C0L0	Oryza sativa subsp. japonica	37%	95%
P0C0L1	Oryza sativa subsp. japonica	37%	98%
P0C0V3	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	49%	84%
P0CP54	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	46%	80%
P0CP55	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	46%	80%
P0CP56	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	42%	96%
P0CP57	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	42%	96%
P12437	Solanum tuberosum	25%	83%
P15003	Solanum lycopersicum	25%	83%
P15004	Solanum lycopersicum	25%	83%
P17180	Armoracia rusticana	24%	87%
P22195	Arachis hypogaea	24%	96%
P24102	Arabidopsis thaliana	24%	87%
P27337	Hordeum vulgare	21%	96%
P48534	Pisum sativum	41%	100%
P80679	Armoracia rusticana	23%	99%
Q01MI9	Oryza sativa subsp. indica	44%	100%
Q02200	Nicotiana sylvestris	25%	94%
Q05431	Arabidopsis thaliana	40%	100%
Q05855	Triticum aestivum	26%	97%
Q0JEQ2	Oryza sativa subsp. japonica	44%	100%
Q10N21	Oryza sativa subsp. japonica	42%	100%
Q1PER6	Arabidopsis thaliana	39%	100%
Q42564	Arabidopsis thaliana	43%	100%
Q42592	Arabidopsis thaliana	36%	81%
Q42593	Arabidopsis thaliana	37%	71%
Q43731	Arabidopsis thaliana	21%	92%
Q43873	Arabidopsis thaliana	22%	92%
Q4HWQ2	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	43%	92%
Q4ING3	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	46%	85%
Q4PBY6	Ustilago maydis (strain 521 / FGSC 9021)	44%	76%
Q4PD66	Ustilago maydis (strain 521 / FGSC 9021)	46%	92%
Q4QC30	Leishmania major	24%	89%
Q4WLG9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	45%	94%
Q4WPF8	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	51%	83%
Q539E5	Hydra viridissima	27%	83%
Q59X94	Candida albicans (strain SC5314 / ATCC MYA-2876)	50%	100%
Q5AEN1	Candida albicans (strain SC5314 / ATCC MYA-2876)	41%	83%
Q5B1Z0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	45%	97%
Q6BIB1	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	48%	71%
Q6BKY9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	43%	84%
Q6C0Z6	Yarrowia lipolytica (strain CLIB 122 / E 150)	42%	89%
Q6C7U1	Yarrowia lipolytica (strain CLIB 122 / E 150)	45%	100%
Q6CAB5	Yarrowia lipolytica (strain CLIB 122 / E 150)	46%	100%
Q6CW24	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	41%	88%
Q6FMG7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	40%	85%
Q6URB0	Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)	48%	80%
Q6ZJJ1	Oryza sativa subsp. japonica	44%	100%
Q7SDV9	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	45%	85%
Q7XJ02	Oryza sativa subsp. japonica	37%	84%
Q7XZP5	Arabidopsis thaliana	42%	100%
Q8GY91	Arabidopsis thaliana	34%	92%
Q96510	Arabidopsis thaliana	23%	92%
Q96518	Arabidopsis thaliana	25%	94%
Q96522	Arabidopsis thaliana	24%	93%
Q9FE01	Oryza sativa subsp. japonica	40%	100%
Q9FJZ9	Arabidopsis thaliana	24%	90%
Q9FL16	Arabidopsis thaliana	30%	92%
Q9FLC0	Arabidopsis thaliana	25%	94%
Q9FX85	Arabidopsis thaliana	24%	87%
Q9LE15	Arabidopsis thaliana	25%	96%
Q9LHA7	Arabidopsis thaliana	30%	96%
Q9M9Q9	Arabidopsis thaliana	23%	94%
Q9SD46	Arabidopsis thaliana	21%	88%
Q9SJZ2	Arabidopsis thaliana	24%	92%
Q9SK52	Arabidopsis thaliana	26%	92%
Q9SY33	Arabidopsis thaliana	25%	87%
Q9SZB9	Arabidopsis thaliana	27%	93%
Q9THX6	Solanum lycopersicum	28%	88%
V5BM56	Trypanosoma cruzi	70%	100%