PseudoU_synth_1 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase, putative

Species:

Leishmania major

UniProt:

Q4Q3H7_LEIMA

TriTrypDb:

LmjF.34.0310 , LMJLV39_340008500 , LMJSD75_340008400

Length:

701

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043231	intracellular membrane-bounded organelle	4	6
GO:0110165	cellular anatomical entity	1	6

Expansion

Sequence features

Q4Q3H7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3H7

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	9
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006396	RNA processing	6	9
GO:0006399	tRNA metabolic process	7	9
GO:0006400	tRNA modification	6	6
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008033	tRNA processing	8	9
GO:0008152	metabolic process	1	9
GO:0009451	RNA modification	5	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0016071	mRNA metabolic process	6	2
GO:0016556	mRNA modification	6	2
GO:0031119	tRNA pseudouridine synthesis	7	6
GO:0034470	ncRNA processing	7	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0034660	ncRNA metabolic process	6	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:1990481	mRNA pseudouridine synthesis	7	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	9
GO:0003723	RNA binding	4	9
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0009982	pseudouridine synthase activity	4	9
GO:0016853	isomerase activity	2	9
GO:0016866	intramolecular transferase activity	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:1901363	heterocyclic compound binding	2	9
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	369	373	PF00656	0.774
CLV_C14_Caspase3-7	374	378	PF00656	0.774
CLV_NRD_NRD_1	219	221	PF00675	0.429
CLV_NRD_NRD_1	282	284	PF00675	0.747
CLV_NRD_NRD_1	289	291	PF00675	0.765
CLV_NRD_NRD_1	298	300	PF00675	0.748
CLV_NRD_NRD_1	53	55	PF00675	0.465
CLV_NRD_NRD_1	578	580	PF00675	0.357
CLV_NRD_NRD_1	603	605	PF00675	0.438
CLV_NRD_NRD_1	608	610	PF00675	0.435
CLV_NRD_NRD_1	631	633	PF00675	0.485
CLV_NRD_NRD_1	665	667	PF00675	0.434
CLV_PCSK_FUR_1	51	55	PF00082	0.571
CLV_PCSK_KEX2_1	145	147	PF00082	0.542
CLV_PCSK_KEX2_1	187	189	PF00082	0.421
CLV_PCSK_KEX2_1	219	221	PF00082	0.475
CLV_PCSK_KEX2_1	264	266	PF00082	0.730
CLV_PCSK_KEX2_1	281	283	PF00082	0.560
CLV_PCSK_KEX2_1	298	300	PF00082	0.601
CLV_PCSK_KEX2_1	50	52	PF00082	0.525
CLV_PCSK_KEX2_1	53	55	PF00082	0.448
CLV_PCSK_KEX2_1	608	610	PF00082	0.436
CLV_PCSK_KEX2_1	631	633	PF00082	0.485
CLV_PCSK_KEX2_1	664	666	PF00082	0.435
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.611
CLV_PCSK_PC1ET2_1	187	189	PF00082	0.503
CLV_PCSK_PC1ET2_1	264	266	PF00082	0.730
CLV_PCSK_PC1ET2_1	298	300	PF00082	0.754
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.558
CLV_PCSK_PC7_1	604	610	PF00082	0.438
CLV_PCSK_SKI1_1	187	191	PF00082	0.417
CLV_PCSK_SKI1_1	269	273	PF00082	0.806
CLV_PCSK_SKI1_1	300	304	PF00082	0.786
CLV_PCSK_SKI1_1	423	427	PF00082	0.355
CLV_PCSK_SKI1_1	439	443	PF00082	0.436
CLV_PCSK_SKI1_1	599	603	PF00082	0.436
CLV_PCSK_SKI1_1	689	693	PF00082	0.490
CLV_PCSK_SKI1_1	697	701	PF00082	0.582
CLV_PCSK_SKI1_1	89	93	PF00082	0.482
CLV_Separin_Metazoa	562	566	PF03568	0.357
DEG_ODPH_VHL_1	245	257	PF01847	0.533
DEG_SCF_FBW7_2	319	326	PF00400	0.698
DOC_CYCLIN_RxL_1	436	446	PF00134	0.567
DOC_CYCLIN_RxL_1	86	95	PF00134	0.482
DOC_MAPK_gen_1	50	60	PF00069	0.442
DOC_MAPK_gen_1	664	672	PF00069	0.407
DOC_MAPK_gen_1	686	694	PF00069	0.407
DOC_MAPK_MEF2A_6	127	136	PF00069	0.385
DOC_MAPK_MEF2A_6	53	62	PF00069	0.405
DOC_MAPK_MEF2A_6	664	672	PF00069	0.386
DOC_PP1_RVXF_1	251	258	PF00149	0.633
DOC_PP1_RVXF_1	691	697	PF00149	0.513
DOC_PP2B_LxvP_1	535	538	PF13499	0.337
DOC_PP4_FxxP_1	244	247	PF00568	0.466
DOC_PP4_FxxP_1	499	502	PF00568	0.438
DOC_USP7_MATH_1	2	6	PF00917	0.699
DOC_USP7_MATH_1	285	289	PF00917	0.710
DOC_USP7_MATH_1	339	343	PF00917	0.696
DOC_USP7_MATH_1	525	529	PF00917	0.415
DOC_WW_Pin1_4	319	324	PF00397	0.763
DOC_WW_Pin1_4	348	353	PF00397	0.748
DOC_WW_Pin1_4	35	40	PF00397	0.543
DOC_WW_Pin1_4	452	457	PF00397	0.357
DOC_WW_Pin1_4	478	483	PF00397	0.412
DOC_WW_Pin1_4	498	503	PF00397	0.162
LIG_14-3-3_CanoR_1	115	123	PF00244	0.439
LIG_14-3-3_CanoR_1	127	133	PF00244	0.382
LIG_14-3-3_CanoR_1	269	274	PF00244	0.721
LIG_14-3-3_CanoR_1	354	360	PF00244	0.716
LIG_14-3-3_CanoR_1	388	394	PF00244	0.508
LIG_14-3-3_CanoR_1	467	473	PF00244	0.366
LIG_14-3-3_CanoR_1	505	511	PF00244	0.438
LIG_Actin_WH2_2	238	255	PF00022	0.611
LIG_Actin_WH2_2	678	695	PF00022	0.464
LIG_AP2alpha_2	476	478	PF02296	0.381
LIG_BRCT_BRCA1_1	474	478	PF00533	0.376
LIG_Clathr_ClatBox_1	110	114	PF01394	0.519
LIG_deltaCOP1_diTrp_1	492	499	PF00928	0.414
LIG_eIF4E_1	597	603	PF01652	0.369
LIG_FHA_1	225	231	PF00498	0.415
LIG_FHA_1	320	326	PF00498	0.797
LIG_FHA_1	335	341	PF00498	0.568
LIG_FHA_1	354	360	PF00498	0.593
LIG_FHA_1	4	10	PF00498	0.542
LIG_FHA_1	646	652	PF00498	0.598
LIG_FHA_1	92	98	PF00498	0.431
LIG_FHA_2	11	17	PF00498	0.743
LIG_FHA_2	230	236	PF00498	0.480
LIG_FHA_2	249	255	PF00498	0.353
LIG_FHA_2	270	276	PF00498	0.788
LIG_FHA_2	507	513	PF00498	0.391
LIG_FHA_2	80	86	PF00498	0.423
LIG_GBD_Chelix_1	668	676	PF00786	0.388
LIG_LIR_Apic_2	222	226	PF02991	0.384
LIG_LIR_Apic_2	24	28	PF02991	0.492
LIG_LIR_Apic_2	243	247	PF02991	0.411
LIG_LIR_Apic_2	426	431	PF02991	0.350
LIG_LIR_Gen_1	227	236	PF02991	0.398
LIG_LIR_Gen_1	405	414	PF02991	0.503
LIG_LIR_Gen_1	448	456	PF02991	0.353
LIG_LIR_Gen_1	622	629	PF02991	0.387
LIG_LIR_Gen_1	99	110	PF02991	0.576
LIG_LIR_Nem_3	207	213	PF02991	0.428
LIG_LIR_Nem_3	239	244	PF02991	0.526
LIG_LIR_Nem_3	38	43	PF02991	0.583
LIG_LIR_Nem_3	448	454	PF02991	0.364
LIG_LIR_Nem_3	464	469	PF02991	0.276
LIG_LIR_Nem_3	622	626	PF02991	0.357
LIG_LIR_Nem_3	67	72	PF02991	0.399
LIG_LIR_Nem_3	79	83	PF02991	0.439
LIG_LIR_Nem_3	99	105	PF02991	0.245
LIG_LYPXL_yS_3	80	83	PF13949	0.464
LIG_MYND_1	28	32	PF01753	0.590
LIG_PCNA_yPIPBox_3	104	118	PF02747	0.521
LIG_RPA_C_Fungi	661	673	PF08784	0.464
LIG_SH2_CRK	201	205	PF00017	0.478
LIG_SH2_CRK	428	432	PF00017	0.509
LIG_SH2_CRK	466	470	PF00017	0.492
LIG_SH2_CRK	69	73	PF00017	0.402
LIG_SH2_GRB2like	213	216	PF00017	0.506
LIG_SH2_GRB2like	451	454	PF00017	0.357
LIG_SH2_NCK_1	201	205	PF00017	0.373
LIG_SH2_SRC	138	141	PF00017	0.510
LIG_SH2_STAP1	221	225	PF00017	0.382
LIG_SH2_STAP1	236	240	PF00017	0.437
LIG_SH2_STAP1	399	403	PF00017	0.576
LIG_SH2_STAP1	510	514	PF00017	0.438
LIG_SH2_STAT5	142	145	PF00017	0.529
LIG_SH2_STAT5	213	216	PF00017	0.463
LIG_SH2_STAT5	224	227	PF00017	0.331
LIG_SH2_STAT5	240	243	PF00017	0.436
LIG_SH2_STAT5	597	600	PF00017	0.369
LIG_SH2_STAT5	623	626	PF00017	0.391
LIG_SH2_STAT5	64	67	PF00017	0.384
LIG_SH2_STAT5	69	72	PF00017	0.393
LIG_SH3_1	26	32	PF00018	0.671
LIG_SH3_1	609	615	PF00018	0.438
LIG_SH3_3	131	137	PF00018	0.367
LIG_SH3_3	244	250	PF00018	0.457
LIG_SH3_3	26	32	PF00018	0.628
LIG_SH3_3	349	355	PF00018	0.815
LIG_SH3_3	471	477	PF00018	0.357
LIG_SH3_3	609	615	PF00018	0.357
LIG_SH3_3	75	81	PF00018	0.476
LIG_SUMO_SIM_anti_2	131	136	PF11976	0.468
LIG_SUMO_SIM_par_1	342	351	PF11976	0.754
LIG_SUMO_SIM_par_1	356	363	PF11976	0.756
LIG_SUMO_SIM_par_1	647	652	PF11976	0.598
LIG_TRAF2_1	13	16	PF00917	0.771
LIG_TRAF2_1	182	185	PF00917	0.493
LIG_TRAF2_1	274	277	PF00917	0.783
LIG_TRAF2_1	481	484	PF00917	0.454
LIG_TRAF2_1	509	512	PF00917	0.422
LIG_TRAF2_1	82	85	PF00917	0.402
LIG_UBA3_1	574	580	PF00899	0.357
LIG_WRC_WIRS_1	225	230	PF05994	0.381
LIG_WRC_WIRS_1	241	246	PF05994	0.445
LIG_WW_3	266	270	PF00397	0.736
MOD_CDK_SPxK_1	348	354	PF00069	0.710
MOD_CDK_SPxK_1	35	41	PF00069	0.543
MOD_CDK_SPxxK_3	452	459	PF00069	0.357
MOD_CDK_SPxxK_3	498	505	PF00069	0.438
MOD_CK1_1	175	181	PF00069	0.443
MOD_CK1_1	248	254	PF00069	0.520
MOD_CK1_1	348	354	PF00069	0.768
MOD_CK1_1	375	381	PF00069	0.732
MOD_CK1_1	490	496	PF00069	0.438
MOD_CK2_1	10	16	PF00069	0.700
MOD_CK2_1	179	185	PF00069	0.542
MOD_CK2_1	229	235	PF00069	0.431
MOD_CK2_1	248	254	PF00069	0.407
MOD_CK2_1	271	277	PF00069	0.791
MOD_CK2_1	329	335	PF00069	0.786
MOD_CK2_1	368	374	PF00069	0.766
MOD_CK2_1	375	381	PF00069	0.690
MOD_CK2_1	478	484	PF00069	0.454
MOD_CK2_1	498	504	PF00069	0.238
MOD_CK2_1	506	512	PF00069	0.367
MOD_CK2_1	525	531	PF00069	0.391
MOD_CK2_1	79	85	PF00069	0.430
MOD_Cter_Amidation	217	220	PF01082	0.480
MOD_Cter_Amidation	262	265	PF01082	0.715
MOD_GlcNHglycan	174	177	PF01048	0.661
MOD_GlcNHglycan	287	290	PF01048	0.818
MOD_GlcNHglycan	330	334	PF01048	0.749
MOD_GlcNHglycan	389	392	PF01048	0.508
MOD_GlcNHglycan	539	542	PF01048	0.436
MOD_GlcNHglycan	625	629	PF01048	0.465
MOD_GSK3_1	175	182	PF00069	0.618
MOD_GSK3_1	236	243	PF00069	0.516
MOD_GSK3_1	285	292	PF00069	0.752
MOD_GSK3_1	3	10	PF00069	0.647
MOD_GSK3_1	329	336	PF00069	0.750
MOD_GSK3_1	368	375	PF00069	0.785
MOD_GSK3_1	457	464	PF00069	0.340
MOD_GSK3_1	468	475	PF00069	0.339
MOD_GSK3_1	91	98	PF00069	0.430
MOD_N-GLC_1	100	105	PF02516	0.499
MOD_N-GLC_1	452	457	PF02516	0.332
MOD_NEK2_1	407	412	PF00069	0.580
MOD_NEK2_1	413	418	PF00069	0.514
MOD_NEK2_1	468	473	PF00069	0.422
MOD_NEK2_1	619	624	PF00069	0.357
MOD_NEK2_2	461	466	PF00069	0.395
MOD_NEK2_2	684	689	PF00069	0.390
MOD_PIKK_1	245	251	PF00454	0.476
MOD_PIKK_1	372	378	PF00454	0.797
MOD_PKA_2	114	120	PF00069	0.441
MOD_PKA_2	289	295	PF00069	0.753
MOD_PKA_2	353	359	PF00069	0.714
MOD_PKA_2	387	393	PF00069	0.585
MOD_Plk_1	461	467	PF00069	0.467
MOD_Plk_2-3	512	518	PF00069	0.492
MOD_Plk_2-3	79	85	PF00069	0.456
MOD_Plk_4	236	242	PF00069	0.567
MOD_Plk_4	407	413	PF00069	0.586
MOD_Plk_4	569	575	PF00069	0.357
MOD_Plk_4	593	599	PF00069	0.422
MOD_Plk_4	619	625	PF00069	0.357
MOD_ProDKin_1	319	325	PF00069	0.767
MOD_ProDKin_1	348	354	PF00069	0.749
MOD_ProDKin_1	35	41	PF00069	0.543
MOD_ProDKin_1	452	458	PF00069	0.357
MOD_ProDKin_1	478	484	PF00069	0.412
MOD_ProDKin_1	498	504	PF00069	0.162
MOD_SUMO_rev_2	15	20	PF00179	0.765
MOD_SUMO_rev_2	182	189	PF00179	0.509
TRG_DiLeu_BaEn_1	185	190	PF01217	0.413
TRG_DiLeu_LyEn_5	185	190	PF01217	0.413
TRG_ENDOCYTIC_2	201	204	PF00928	0.395
TRG_ENDOCYTIC_2	42	45	PF00928	0.517
TRG_ENDOCYTIC_2	451	454	PF00928	0.357
TRG_ENDOCYTIC_2	466	469	PF00928	0.357
TRG_ENDOCYTIC_2	623	626	PF00928	0.391
TRG_ENDOCYTIC_2	69	72	PF00928	0.393
TRG_ENDOCYTIC_2	80	83	PF00928	0.430
TRG_ER_diArg_1	219	221	PF00400	0.475
TRG_ER_diArg_1	281	283	PF00400	0.686
TRG_ER_diArg_1	51	54	PF00400	0.575
TRG_ER_diArg_1	607	609	PF00400	0.436
TRG_ER_diArg_1	631	633	PF00400	0.487
TRG_ER_diArg_1	664	666	PF00400	0.473
TRG_NLS_Bipartite_1	281	302	PF00514	0.756
TRG_NLS_MonoCore_2	282	287	PF00514	0.767
TRG_NLS_MonoExtC_3	49	54	PF00514	0.569
TRG_NLS_MonoExtN_4	281	287	PF00514	0.759
TRG_NLS_MonoExtN_4	297	302	PF00514	0.716
TRG_NLS_MonoExtN_4	51	58	PF00514	0.456
TRG_Pf-PMV_PEXEL_1	188	192	PF00026	0.459
TRG_Pf-PMV_PEXEL_1	439	444	PF00026	0.567
TRG_Pf-PMV_PEXEL_1	89	93	PF00026	0.482

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I609	Leptomonas seymouri	68%	100%
A0A3Q8IKM7	Leishmania donovani	95%	100%
A0A422N797	Trypanosoma rangeli	42%	100%
A4H7D9	Leishmania braziliensis	81%	92%
A4I9K1	Leishmania infantum	95%	100%
D0A1W0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9B4J9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%