Putative ubiquitin-conjugating enzyme e2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania major

UniProt:

Q4Q3B5_LEIMA

TriTrypDb:

LmjF.34.0900 , LMJLV39_340016000 , LMJSD75_340015800

Length:

289

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005783	endoplasmic reticulum	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4Q3B5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q3B5

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010033	response to organic substance	3	2
GO:0010243	response to organonitrogen compound	4	2
GO:0010498	proteasomal protein catabolic process	5	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0030163	protein catabolic process	4	2
GO:0030433	ubiquitin-dependent ERAD pathway	6	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0033554	cellular response to stress	3	2
GO:0034976	response to endoplasmic reticulum stress	4	2
GO:0036211	protein modification process	4	2
GO:0036503	ERAD pathway	5	2
GO:0042221	response to chemical	2	2
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0050896	response to stimulus	1	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0051716	cellular response to stimulus	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901565	organonitrogen compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2
GO:1901698	response to nitrogen compound	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0016874	ligase activity	2	7
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061631	ubiquitin conjugating enzyme activity	5	2
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	135	137	PF00675	0.327
CLV_NRD_NRD_1	141	143	PF00675	0.277
CLV_NRD_NRD_1	16	18	PF00675	0.378
CLV_NRD_NRD_1	220	222	PF00675	0.502
CLV_NRD_NRD_1	269	271	PF00675	0.324
CLV_PCSK_KEX2_1	10	12	PF00082	0.385
CLV_PCSK_KEX2_1	134	136	PF00082	0.330
CLV_PCSK_KEX2_1	147	149	PF00082	0.298
CLV_PCSK_KEX2_1	154	156	PF00082	0.222
CLV_PCSK_KEX2_1	244	246	PF00082	0.512
CLV_PCSK_KEX2_1	269	271	PF00082	0.324
CLV_PCSK_PC1ET2_1	10	12	PF00082	0.330
CLV_PCSK_PC1ET2_1	147	149	PF00082	0.406
CLV_PCSK_PC1ET2_1	154	156	PF00082	0.330
CLV_PCSK_PC1ET2_1	244	246	PF00082	0.454
CLV_PCSK_SKI1_1	107	111	PF00082	0.330
CLV_PCSK_SKI1_1	154	158	PF00082	0.432
DEG_APCC_DBOX_1	106	114	PF00400	0.530
DOC_CKS1_1	75	80	PF01111	0.575
DOC_MAPK_gen_1	10	16	PF00069	0.555
DOC_MAPK_gen_1	269	277	PF00069	0.530
DOC_MAPK_gen_1	81	90	PF00069	0.530
DOC_MAPK_HePTP_8	266	278	PF00069	0.530
DOC_MAPK_MEF2A_6	269	278	PF00069	0.530
DOC_MAPK_MEF2A_6	81	90	PF00069	0.530
DOC_MAPK_RevD_3	4	18	PF00069	0.402
DOC_PP4_FxxP_1	68	71	PF00568	0.530
DOC_USP7_MATH_1	112	116	PF00917	0.561
DOC_USP7_MATH_1	138	142	PF00917	0.547
DOC_USP7_MATH_1	209	213	PF00917	0.687
DOC_USP7_MATH_1	240	244	PF00917	0.659
DOC_USP7_UBL2_3	143	147	PF12436	0.530
DOC_WW_Pin1_4	171	176	PF00397	0.702
DOC_WW_Pin1_4	220	225	PF00397	0.794
DOC_WW_Pin1_4	46	51	PF00397	0.554
DOC_WW_Pin1_4	74	79	PF00397	0.547
DOC_WW_Pin1_4	98	103	PF00397	0.530
LIG_14-3-3_CanoR_1	269	275	PF00244	0.530
LIG_deltaCOP1_diTrp_1	22	27	PF00928	0.462
LIG_deltaCOP1_diTrp_1	98	104	PF00928	0.530
LIG_DLG_GKlike_1	270	277	PF00625	0.397
LIG_FHA_1	106	112	PF00498	0.530
LIG_FHA_1	163	169	PF00498	0.545
LIG_FHA_1	254	260	PF00498	0.739
LIG_FHA_2	128	134	PF00498	0.530
LIG_FHA_2	189	195	PF00498	0.733
LIG_FHA_2	209	215	PF00498	0.625
LIG_FHA_2	47	53	PF00498	0.530
LIG_LIR_Apic_2	22	26	PF02991	0.461
LIG_LIR_Apic_2	65	71	PF02991	0.530
LIG_PCNA_PIPBox_1	34	43	PF02747	0.530
LIG_PCNA_yPIPBox_3	28	41	PF02747	0.515
LIG_Pex14_1	23	27	PF04695	0.462
LIG_PTB_Apo_2	89	96	PF02174	0.530
LIG_PTB_Phospho_1	89	95	PF10480	0.530
LIG_SH2_CRK	153	157	PF00017	0.578
LIG_SH2_CRK	271	275	PF00017	0.397
LIG_SH2_PTP2	56	59	PF00017	0.599
LIG_SH2_STAP1	271	275	PF00017	0.397
LIG_SH2_STAT5	40	43	PF00017	0.544
LIG_SH2_STAT5	56	59	PF00017	0.465
LIG_SH3_3	213	219	PF00018	0.697
LIG_SH3_3	64	70	PF00018	0.530
LIG_SUMO_SIM_anti_2	273	279	PF11976	0.397
LIG_SUMO_SIM_par_1	108	115	PF11976	0.530
LIG_SUMO_SIM_par_1	58	65	PF11976	0.530
LIG_TRAF2_1	130	133	PF00917	0.490
LIG_TRAF2_1	205	208	PF00917	0.705
LIG_WRC_WIRS_1	113	118	PF05994	0.530
MOD_CDK_SPK_2	171	176	PF00069	0.633
MOD_CDK_SPxxK_3	74	81	PF00069	0.547
MOD_CK1_1	180	186	PF00069	0.758
MOD_CK1_1	212	218	PF00069	0.760
MOD_CK1_1	243	249	PF00069	0.696
MOD_CK1_1	91	97	PF00069	0.530
MOD_CK2_1	127	133	PF00069	0.578
MOD_CK2_1	188	194	PF00069	0.738
MOD_CK2_1	208	214	PF00069	0.618
MOD_CK2_1	46	52	PF00069	0.588
MOD_GlcNHglycan	127	130	PF01048	0.355
MOD_GlcNHglycan	180	183	PF01048	0.549
MOD_GlcNHglycan	225	228	PF01048	0.443
MOD_GlcNHglycan	78	81	PF01048	0.330
MOD_GSK3_1	112	119	PF00069	0.535
MOD_GSK3_1	121	128	PF00069	0.474
MOD_GSK3_1	177	184	PF00069	0.716
MOD_GSK3_1	208	215	PF00069	0.763
MOD_GSK3_1	231	238	PF00069	0.757
MOD_LATS_1	251	257	PF00433	0.686
MOD_N-GLC_1	91	96	PF02516	0.330
MOD_N-GLC_1	98	103	PF02516	0.272
MOD_NEK2_1	90	95	PF00069	0.530
MOD_NEK2_2	112	117	PF00069	0.530
MOD_PK_1	69	75	PF00069	0.565
MOD_PKA_1	252	258	PF00069	0.622
MOD_Plk_1	91	97	PF00069	0.558
MOD_Plk_2-3	188	194	PF00069	0.739
MOD_Plk_4	112	118	PF00069	0.530
MOD_Plk_4	270	276	PF00069	0.445
MOD_ProDKin_1	171	177	PF00069	0.710
MOD_ProDKin_1	220	226	PF00069	0.795
MOD_ProDKin_1	46	52	PF00069	0.554
MOD_ProDKin_1	74	80	PF00069	0.547
MOD_ProDKin_1	98	104	PF00069	0.530
MOD_SUMO_for_1	175	178	PF00179	0.686
TRG_ENDOCYTIC_2	153	156	PF00928	0.582
TRG_ENDOCYTIC_2	271	274	PF00928	0.397
TRG_ENDOCYTIC_2	40	43	PF00928	0.544
TRG_ENDOCYTIC_2	56	59	PF00928	0.465
TRG_ER_diArg_1	134	136	PF00400	0.462
TRG_ER_diArg_1	268	270	PF00400	0.655
TRG_Pf-PMV_PEXEL_1	155	159	PF00026	0.392
TRG_Pf-PMV_PEXEL_1	17	22	PF00026	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IHC0	Leishmania donovani	95%	100%
A0A3Q8IMQ9	Leishmania donovani	27%	100%
A4HAK3	Leishmania braziliensis	80%	100%
A4HAK5	Leishmania braziliensis	80%	100%
A4HAK6	Leishmania braziliensis	81%	100%
A4I9B7	Leishmania infantum	27%	100%
A4I9P7	Leishmania infantum	95%	100%
C9ZU73	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	81%
E9B4R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	99%
P33296	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	39%	100%
Q4Q3Q8	Leishmania major	28%	100%
Q6CMG6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	36%	100%