EAP30 family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

EAP30/Vps36 family, putative

Species:

Leishmania major

UniProt:

Q4Q355_LEIMA

TriTrypDb:

LmjF.34.1470 , LMJLV39_340023000 , LMJSD75_340023100

Length:

394

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	12
GO:0005737	cytoplasm	2	2
GO:0032991	protein-containing complex	1	12
GO:0036452	ESCRT complex	2	12
GO:0098796	membrane protein complex	2	12
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q355

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q355

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0006886	intracellular protein transport	4	2
GO:0007034	vacuolar transport	4	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016192	vesicle-mediated transport	4	12
GO:0016197	endosomal transport	4	2
GO:0032509	endosome transport via multivesicular body sorting pathway	5	2
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	2
GO:0045184	establishment of protein localization	3	2
GO:0045324	late endosome to vacuole transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071985	multivesicular body sorting pathway	5	12
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072665	protein localization to vacuole	6	2
GO:0072666	establishment of protein localization to vacuole	5	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	202	206	PF00656	0.441
CLV_NRD_NRD_1	110	112	PF00675	0.249
CLV_NRD_NRD_1	161	163	PF00675	0.340
CLV_NRD_NRD_1	3	5	PF00675	0.509
CLV_NRD_NRD_1	368	370	PF00675	0.730
CLV_PCSK_KEX2_1	161	163	PF00082	0.295
CLV_PCSK_KEX2_1	166	168	PF00082	0.259
CLV_PCSK_KEX2_1	2	4	PF00082	0.527
CLV_PCSK_KEX2_1	319	321	PF00082	0.341
CLV_PCSK_KEX2_1	368	370	PF00082	0.642
CLV_PCSK_PC1ET2_1	166	168	PF00082	0.268
CLV_PCSK_PC1ET2_1	319	321	PF00082	0.327
CLV_PCSK_PC1ET2_1	368	370	PF00082	0.635
CLV_PCSK_PC7_1	162	168	PF00082	0.240
CLV_PCSK_PC7_1	315	321	PF00082	0.339
CLV_PCSK_SKI1_1	315	319	PF00082	0.353
CLV_PCSK_SKI1_1	83	87	PF00082	0.248
DEG_APCC_DBOX_1	314	322	PF00400	0.539
DEG_Nend_UBRbox_1	1	4	PF02207	0.520
DOC_CKS1_1	201	206	PF01111	0.448
DOC_PP1_RVXF_1	120	126	PF00149	0.539
DOC_USP7_MATH_1	149	153	PF00917	0.405
DOC_USP7_MATH_1	246	250	PF00917	0.559
DOC_USP7_MATH_1	285	289	PF00917	0.463
DOC_USP7_MATH_1	350	354	PF00917	0.741
DOC_USP7_UBL2_3	364	368	PF12436	0.639
DOC_WW_Pin1_4	200	205	PF00397	0.448
DOC_WW_Pin1_4	346	351	PF00397	0.709
LIG_14-3-3_CanoR_1	167	176	PF00244	0.456
LIG_APCC_ABBAyCdc20_2	82	88	PF00400	0.491
LIG_BIR_III_4	88	92	PF00653	0.491
LIG_BRCT_BRCA1_1	287	291	PF00533	0.457
LIG_eIF4E_1	131	137	PF01652	0.491
LIG_FHA_1	324	330	PF00498	0.539
LIG_FHA_1	358	364	PF00498	0.683
LIG_FHA_1	370	376	PF00498	0.666
LIG_FHA_2	200	206	PF00498	0.448
LIG_FHA_2	251	257	PF00498	0.430
LIG_GBD_Chelix_1	70	78	PF00786	0.304
LIG_LIR_Nem_3	382	388	PF02991	0.418
LIG_LIR_Nem_3	94	99	PF02991	0.440
LIG_PALB2_WD40_1	382	390	PF16756	0.497
LIG_REV1ctd_RIR_1	123	133	PF16727	0.539
LIG_SH2_CRK	189	193	PF00017	0.458
LIG_SH2_PTP2	117	120	PF00017	0.448
LIG_SH2_STAT5	117	120	PF00017	0.441
LIG_SH2_STAT5	199	202	PF00017	0.437
LIG_SH2_STAT5	218	221	PF00017	0.504
LIG_SH3_1	240	246	PF00018	0.388
LIG_SH3_3	240	246	PF00018	0.475
LIG_SH3_3	284	290	PF00018	0.453
LIG_SUMO_SIM_anti_2	180	186	PF11976	0.539
LIG_SUMO_SIM_anti_2	211	217	PF11976	0.530
LIG_TRAF2_1	234	237	PF00917	0.572
LIG_TRAF2_1	359	362	PF00917	0.728
LIG_TYR_ITIM	383	388	PF00017	0.538
LIG_UBA3_1	175	181	PF00899	0.539
MOD_CDC14_SPxK_1	349	352	PF00782	0.667
MOD_CDK_SPxK_1	346	352	PF00069	0.702
MOD_CK1_1	154	160	PF00069	0.519
MOD_CK1_1	180	186	PF00069	0.515
MOD_CK1_1	283	289	PF00069	0.443
MOD_CK1_1	353	359	PF00069	0.709
MOD_CK1_1	55	61	PF00069	0.498
MOD_CK2_1	355	361	PF00069	0.737
MOD_Cter_Amidation	164	167	PF01082	0.276
MOD_GlcNHglycan	151	154	PF01048	0.340
MOD_GlcNHglycan	240	243	PF01048	0.305
MOD_GlcNHglycan	247	251	PF01048	0.291
MOD_GlcNHglycan	256	260	PF01048	0.305
MOD_GlcNHglycan	261	264	PF01048	0.341
MOD_GlcNHglycan	304	307	PF01048	0.352
MOD_GlcNHglycan	334	338	PF01048	0.654
MOD_GSK3_1	132	139	PF00069	0.509
MOD_GSK3_1	149	156	PF00069	0.534
MOD_GSK3_1	195	202	PF00069	0.470
MOD_GSK3_1	246	253	PF00069	0.576
MOD_GSK3_1	255	262	PF00069	0.548
MOD_GSK3_1	285	292	PF00069	0.509
MOD_GSK3_1	298	305	PF00069	0.539
MOD_GSK3_1	329	336	PF00069	0.623
MOD_GSK3_1	346	353	PF00069	0.707
MOD_NEK2_1	136	141	PF00069	0.458
MOD_NEK2_1	70	75	PF00069	0.441
MOD_NEK2_1	78	83	PF00069	0.431
MOD_PIKK_1	289	295	PF00454	0.515
MOD_PIKK_1	298	304	PF00454	0.470
MOD_PIKK_1	329	335	PF00454	0.704
MOD_PKA_1	369	375	PF00069	0.721
MOD_PKA_2	280	286	PF00069	0.472
MOD_PKA_2	298	304	PF00069	0.491
MOD_PKA_2	338	344	PF00069	0.776
MOD_Plk_1	131	137	PF00069	0.539
MOD_Plk_1	283	289	PF00069	0.409
MOD_Plk_4	132	138	PF00069	0.564
MOD_Plk_4	180	186	PF00069	0.536
MOD_Plk_4	195	201	PF00069	0.510
MOD_Plk_4	323	329	PF00069	0.422
MOD_ProDKin_1	200	206	PF00069	0.448
MOD_ProDKin_1	346	352	PF00069	0.709
MOD_SUMO_rev_2	105	113	PF00179	0.448
MOD_SUMO_rev_2	160	168	PF00179	0.478
MOD_SUMO_rev_2	358	366	PF00179	0.619
MOD_SUMO_rev_2	376	383	PF00179	0.382
TRG_ENDOCYTIC_2	117	120	PF00928	0.458
TRG_ENDOCYTIC_2	189	192	PF00928	0.434
TRG_ENDOCYTIC_2	385	388	PF00928	0.397
TRG_ENDOCYTIC_2	96	99	PF00928	0.448
TRG_ER_diArg_1	1	4	PF00400	0.520
TRG_ER_diArg_1	63	66	PF00400	0.460
TRG_Pf-PMV_PEXEL_1	68	72	PF00026	0.280
TRG_PTS2	1	39	PF00400	0.334

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HSW5	Leptomonas seymouri	70%	100%
A0A0S4J518	Bodo saltans	28%	96%
A0A1X0P9K2	Trypanosomatidae	45%	100%
A0A3Q8IKY6	Leishmania donovani	92%	100%
A0A3R7NWX2	Trypanosoma rangeli	42%	100%
A4H7S7	Leishmania braziliensis	77%	99%
A4I9V9	Leishmania infantum	92%	100%
C9ZMT0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9B4X2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	99%
V5BT20	Trypanosoma cruzi	41%	100%