Endo/exonuclease/phosphatase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Endonuclease/Exonuclease/phosphatase family, putative

Species:

Leishmania major

UniProt:

Q4Q2X8_LEIMA

TriTrypDb:

LmjF.34.2220 , LMJLV39_340026900 , LMJSD75_340027800

Length:

705

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q2X8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2X8

Function

Biological processes
Term	Name	Level	Count
GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	6	2
GO:0000956	nuclear-transcribed mRNA catabolic process	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006401	RNA catabolic process	5	2
GO:0006402	mRNA catabolic process	6	2
GO:0006417	regulation of translation	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009890	negative regulation of biosynthetic process	5	2
GO:0009892	negative regulation of metabolic process	4	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009894	regulation of catabolic process	4	2
GO:0009896	positive regulation of catabolic process	5	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010558	negative regulation of macromolecule biosynthetic process	6	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0010605	negative regulation of macromolecule metabolic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0010629	negative regulation of gene expression	6	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0017148	negative regulation of translation	7	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019439	aromatic compound catabolic process	4	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031324	negative regulation of cellular metabolic process	5	2
GO:0031325	positive regulation of cellular metabolic process	5	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0031327	negative regulation of cellular biosynthetic process	6	2
GO:0031329	regulation of cellular catabolic process	5	2
GO:0031331	positive regulation of cellular catabolic process	6	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0034249	negative regulation of amide metabolic process	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043487	regulation of RNA stability	3	2
GO:0043488	regulation of mRNA stability	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046700	heterocycle catabolic process	4	2
GO:0048518	positive regulation of biological process	3	2
GO:0048519	negative regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0048523	negative regulation of cellular process	4	2
GO:0050779	RNA destabilization	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051172	negative regulation of nitrogen compound metabolic process	5	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0051248	negative regulation of protein metabolic process	6	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051254	positive regulation of RNA metabolic process	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0061013	regulation of mRNA catabolic process	6	2
GO:0061014	positive regulation of mRNA catabolic process	7	2
GO:0061157	mRNA destabilization	5	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0071704	organic substance metabolic process	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2
GO:1903311	regulation of mRNA metabolic process	6	2
GO:1903313	positive regulation of mRNA metabolic process	7	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2
GO:2000113	obsolete negative regulation of cellular macromolecule biosynthetic process	7	2

Molecular functions
Term	Name	Level	Count
GO:0000175	3'-5'-RNA exonuclease activity	7	2
GO:0003824	catalytic activity	1	12
GO:0004518	nuclease activity	4	3
GO:0004527	exonuclease activity	5	3
GO:0004532	RNA exonuclease activity	5	2
GO:0004540	RNA nuclease activity	4	2
GO:0008408	3'-5' exonuclease activity	6	2
GO:0016787	hydrolase activity	2	3
GO:0016788	hydrolase activity, acting on ester bonds	3	3
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	2
GO:0016896	RNA exonuclease activity, producing 5'-phosphomonoesters	6	2
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:0004519	endonuclease activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	406	412	PF00089	0.361
CLV_NRD_NRD_1	320	322	PF00675	0.349
CLV_NRD_NRD_1	496	498	PF00675	0.413
CLV_NRD_NRD_1	566	568	PF00675	0.406
CLV_NRD_NRD_1	615	617	PF00675	0.218
CLV_PCSK_FUR_1	494	498	PF00082	0.370
CLV_PCSK_KEX2_1	40	42	PF00082	0.722
CLV_PCSK_KEX2_1	496	498	PF00082	0.412
CLV_PCSK_KEX2_1	571	573	PF00082	0.401
CLV_PCSK_KEX2_1	80	82	PF00082	0.549
CLV_PCSK_PC1ET2_1	40	42	PF00082	0.722
CLV_PCSK_PC1ET2_1	571	573	PF00082	0.413
CLV_PCSK_PC1ET2_1	80	82	PF00082	0.573
CLV_PCSK_PC7_1	492	498	PF00082	0.374
CLV_PCSK_PC7_1	567	573	PF00082	0.413
CLV_PCSK_SKI1_1	286	290	PF00082	0.323
CLV_PCSK_SKI1_1	344	348	PF00082	0.340
CLV_PCSK_SKI1_1	417	421	PF00082	0.307
CLV_PCSK_SKI1_1	429	433	PF00082	0.339
CLV_PCSK_SKI1_1	515	519	PF00082	0.304
CLV_PCSK_SKI1_1	616	620	PF00082	0.416
CLV_PCSK_SKI1_1	66	70	PF00082	0.508
CLV_PCSK_SKI1_1	80	84	PF00082	0.576
CLV_Separin_Metazoa	665	669	PF03568	0.425
DEG_Nend_UBRbox_3	1	3	PF02207	0.604
DEG_SPOP_SBC_1	116	120	PF00917	0.394
DOC_MAPK_MEF2A_6	239	247	PF00069	0.488
DOC_MAPK_MEF2A_6	60	69	PF00069	0.465
DOC_PP1_RVXF_1	140	146	PF00149	0.423
DOC_PP1_RVXF_1	64	70	PF00149	0.565
DOC_PP2B_LxvP_1	113	116	PF13499	0.558
DOC_PP2B_LxvP_1	266	269	PF13499	0.566
DOC_PP2B_LxvP_1	639	642	PF13499	0.442
DOC_PP2B_LxvP_1	68	71	PF13499	0.555
DOC_PP4_FxxP_1	682	685	PF00568	0.425
DOC_PP4_FxxP_1	69	72	PF00568	0.583
DOC_USP7_MATH_1	12	16	PF00917	0.488
DOC_USP7_MATH_1	185	189	PF00917	0.405
DOC_USP7_MATH_1	422	426	PF00917	0.439
DOC_USP7_MATH_1	485	489	PF00917	0.421
DOC_USP7_MATH_1	593	597	PF00917	0.413
DOC_USP7_MATH_1	605	609	PF00917	0.426
DOC_USP7_MATH_1	671	675	PF00917	0.399
DOC_USP7_MATH_1	72	76	PF00917	0.686
DOC_USP7_UBL2_3	571	575	PF12436	0.374
DOC_USP7_UBL2_3	80	84	PF12436	0.646
DOC_WW_Pin1_4	169	174	PF00397	0.520
DOC_WW_Pin1_4	228	233	PF00397	0.387
DOC_WW_Pin1_4	473	478	PF00397	0.420
DOC_WW_Pin1_4	481	486	PF00397	0.350
DOC_WW_Pin1_4	573	578	PF00397	0.427
DOC_WW_Pin1_4	581	586	PF00397	0.414
LIG_14-3-3_CanoR_1	244	248	PF00244	0.492
LIG_14-3-3_CanoR_1	286	291	PF00244	0.411
LIG_14-3-3_CanoR_1	41	47	PF00244	0.625
LIG_14-3-3_CanoR_1	515	522	PF00244	0.425
LIG_14-3-3_CanoR_1	99	106	PF00244	0.607
LIG_Actin_WH2_2	403	419	PF00022	0.365
LIG_APCC_ABBA_1	8	13	PF00400	0.585
LIG_APCC_ABBAyCdc20_2	84	90	PF00400	0.615
LIG_BRCT_BRCA1_1	187	191	PF00533	0.491
LIG_BRCT_BRCA1_1	195	199	PF00533	0.486
LIG_BRCT_BRCA1_1	399	403	PF00533	0.466
LIG_eIF4E_1	545	551	PF01652	0.335
LIG_FHA_1	116	122	PF00498	0.588
LIG_FHA_1	17	23	PF00498	0.437
LIG_FHA_1	231	237	PF00498	0.418
LIG_FHA_1	275	281	PF00498	0.536
LIG_FHA_1	287	293	PF00498	0.221
LIG_FHA_1	310	316	PF00498	0.281
LIG_FHA_1	41	47	PF00498	0.660
LIG_FHA_1	517	523	PF00498	0.389
LIG_FHA_1	667	673	PF00498	0.310
LIG_FHA_1	91	97	PF00498	0.529
LIG_FHA_2	582	588	PF00498	0.375
LIG_FHA_2	604	610	PF00498	0.270
LIG_FHA_2	647	653	PF00498	0.343
LIG_GBD_Chelix_1	696	704	PF00786	0.304
LIG_LIR_Apic_2	159	165	PF02991	0.311
LIG_LIR_Apic_2	349	353	PF02991	0.413
LIG_LIR_Apic_2	679	685	PF02991	0.426
LIG_LIR_Gen_1	129	140	PF02991	0.542
LIG_LIR_Gen_1	200	209	PF02991	0.559
LIG_LIR_Gen_1	218	228	PF02991	0.332
LIG_LIR_Gen_1	251	262	PF02991	0.443
LIG_LIR_Gen_1	289	297	PF02991	0.304
LIG_LIR_Gen_1	381	392	PF02991	0.364
LIG_LIR_Gen_1	640	650	PF02991	0.389
LIG_LIR_Gen_1	655	664	PF02991	0.219
LIG_LIR_Nem_3	129	135	PF02991	0.489
LIG_LIR_Nem_3	200	204	PF02991	0.482
LIG_LIR_Nem_3	218	224	PF02991	0.519
LIG_LIR_Nem_3	279	285	PF02991	0.395
LIG_LIR_Nem_3	289	293	PF02991	0.281
LIG_LIR_Nem_3	345	350	PF02991	0.348
LIG_LIR_Nem_3	381	387	PF02991	0.364
LIG_LIR_Nem_3	400	406	PF02991	0.181
LIG_LIR_Nem_3	511	517	PF02991	0.315
LIG_LIR_Nem_3	640	646	PF02991	0.315
LIG_LIR_Nem_3	647	653	PF02991	0.290
LIG_LIR_Nem_3	655	659	PF02991	0.271
LIG_NRBOX	549	555	PF00104	0.419
LIG_PCNA_yPIPBox_3	149	161	PF02747	0.439
LIG_Pex14_2	347	351	PF04695	0.396
LIG_Pex14_2	646	650	PF04695	0.425
LIG_PTB_Apo_2	138	145	PF02174	0.365
LIG_PTB_Apo_2	683	690	PF02174	0.320
LIG_PTB_Phospho_1	683	689	PF10480	0.320
LIG_SH2_CRK	162	166	PF00017	0.423
LIG_SH2_CRK	290	294	PF00017	0.304
LIG_SH2_CRK	360	364	PF00017	0.389
LIG_SH2_CRK	463	467	PF00017	0.320
LIG_SH2_CRK	514	518	PF00017	0.320
LIG_SH2_NCK_1	162	166	PF00017	0.410
LIG_SH2_PTP2	177	180	PF00017	0.384
LIG_SH2_STAT3	624	627	PF00017	0.451
LIG_SH2_STAT5	177	180	PF00017	0.351
LIG_SH2_STAT5	350	353	PF00017	0.365
LIG_SH2_STAT5	364	367	PF00017	0.221
LIG_SH2_STAT5	656	659	PF00017	0.349
LIG_SH3_1	492	498	PF00018	0.335
LIG_SH3_2	36	41	PF14604	0.702
LIG_SH3_3	118	124	PF00018	0.525
LIG_SH3_3	167	173	PF00018	0.405
LIG_SH3_3	175	181	PF00018	0.353
LIG_SH3_3	23	29	PF00018	0.257
LIG_SH3_3	265	271	PF00018	0.482
LIG_SH3_3	277	283	PF00018	0.358
LIG_SH3_3	33	39	PF00018	0.741
LIG_SH3_3	492	498	PF00018	0.419
LIG_SH3_3	582	588	PF00018	0.335
LIG_SH3_3	6	12	PF00018	0.420
LIG_SH3_3	667	673	PF00018	0.314
LIG_SH3_CIN85_PxpxPR_1	267	272	PF14604	0.489
LIG_SUMO_SIM_anti_2	155	162	PF11976	0.297
LIG_SUMO_SIM_anti_2	186	191	PF11976	0.517
LIG_SUMO_SIM_anti_2	548	555	PF11976	0.335
LIG_SUMO_SIM_anti_2	660	669	PF11976	0.327
LIG_SUMO_SIM_anti_2	691	699	PF11976	0.353
LIG_SUMO_SIM_par_1	15	21	PF11976	0.436
LIG_SUMO_SIM_par_1	166	172	PF11976	0.548
LIG_TRAF2_1	51	54	PF00917	0.575
LIG_TYR_ITIM	175	180	PF00017	0.471
LIG_TYR_ITIM	288	293	PF00017	0.320
LIG_UBA3_1	315	322	PF00899	0.372
LIG_WW_3	70	74	PF00397	0.620
MOD_CK1_1	169	175	PF00069	0.520
MOD_CK1_1	193	199	PF00069	0.425
MOD_CK1_1	205	211	PF00069	0.567
MOD_CK1_1	300	306	PF00069	0.342
MOD_CK1_1	421	427	PF00069	0.396
MOD_CK1_1	596	602	PF00069	0.401
MOD_CK1_1	660	666	PF00069	0.375
MOD_CK1_1	75	81	PF00069	0.707
MOD_CK2_1	122	128	PF00069	0.573
MOD_CK2_1	367	373	PF00069	0.410
MOD_CK2_1	434	440	PF00069	0.339
MOD_CK2_1	573	579	PF00069	0.411
MOD_CK2_1	603	609	PF00069	0.344
MOD_CK2_1	646	652	PF00069	0.343
MOD_CK2_1	671	677	PF00069	0.413
MOD_GlcNHglycan	206	210	PF01048	0.635
MOD_GlcNHglycan	212	215	PF01048	0.677
MOD_GlcNHglycan	367	370	PF01048	0.368
MOD_GlcNHglycan	420	423	PF01048	0.381
MOD_GlcNHglycan	436	439	PF01048	0.334
MOD_GlcNHglycan	74	77	PF01048	0.615
MOD_GSK3_1	12	19	PF00069	0.464
MOD_GSK3_1	122	129	PF00069	0.582
MOD_GSK3_1	130	137	PF00069	0.374
MOD_GSK3_1	204	211	PF00069	0.460
MOD_GSK3_1	226	233	PF00069	0.511
MOD_GSK3_1	248	255	PF00069	0.434
MOD_GSK3_1	296	303	PF00069	0.394
MOD_GSK3_1	40	47	PF00069	0.643
MOD_GSK3_1	418	425	PF00069	0.394
MOD_GSK3_1	481	488	PF00069	0.388
MOD_GSK3_1	54	61	PF00069	0.515
MOD_GSK3_1	573	580	PF00069	0.390
MOD_GSK3_1	637	644	PF00069	0.423
MOD_GSK3_1	71	78	PF00069	0.673
MOD_N-GLC_1	153	158	PF02516	0.496
MOD_N-GLC_1	365	370	PF02516	0.389
MOD_N-GLC_1	505	510	PF02516	0.304
MOD_N-GLC_1	596	601	PF02516	0.468
MOD_N-GLC_1	644	649	PF02516	0.339
MOD_N-GLC_2	453	455	PF02516	0.304
MOD_NEK2_1	126	131	PF00069	0.565
MOD_NEK2_1	204	209	PF00069	0.379
MOD_NEK2_1	297	302	PF00069	0.320
MOD_NEK2_1	379	384	PF00069	0.408
MOD_NEK2_1	432	437	PF00069	0.460
MOD_NEK2_1	505	510	PF00069	0.322
MOD_NEK2_1	516	521	PF00069	0.311
MOD_NEK2_1	646	651	PF00069	0.397
MOD_NEK2_1	700	705	PF00069	0.456
MOD_PIKK_1	12	18	PF00454	0.600
MOD_PIKK_1	596	602	PF00454	0.438
MOD_PIKK_1	603	609	PF00454	0.401
MOD_PK_1	367	373	PF00069	0.425
MOD_PKA_1	40	46	PF00069	0.673
MOD_PKA_1	80	86	PF00069	0.538
MOD_PKA_2	243	249	PF00069	0.496
MOD_PKA_2	379	385	PF00069	0.358
MOD_PKA_2	40	46	PF00069	0.658
MOD_PKA_2	432	438	PF00069	0.449
MOD_PKA_2	72	78	PF00069	0.701
MOD_PKA_2	80	86	PF00069	0.365
MOD_PKA_2	98	104	PF00069	0.535
MOD_Plk_1	126	132	PF00069	0.538
MOD_Plk_1	153	159	PF00069	0.437
MOD_Plk_1	185	191	PF00069	0.469
MOD_Plk_1	20	26	PF00069	0.460
MOD_Plk_1	470	476	PF00069	0.468
MOD_Plk_1	637	643	PF00069	0.347
MOD_Plk_1	644	650	PF00069	0.289
MOD_Plk_1	660	666	PF00069	0.339
MOD_Plk_2-3	525	531	PF00069	0.374
MOD_Plk_4	117	123	PF00069	0.489
MOD_Plk_4	153	159	PF00069	0.400
MOD_Plk_4	163	169	PF00069	0.396
MOD_Plk_4	185	191	PF00069	0.485
MOD_Plk_4	346	352	PF00069	0.346
MOD_Plk_4	398	404	PF00069	0.408
MOD_Plk_4	470	476	PF00069	0.451
MOD_ProDKin_1	169	175	PF00069	0.521
MOD_ProDKin_1	228	234	PF00069	0.392
MOD_ProDKin_1	473	479	PF00069	0.420
MOD_ProDKin_1	481	487	PF00069	0.350
MOD_ProDKin_1	573	579	PF00069	0.427
MOD_ProDKin_1	581	587	PF00069	0.414
MOD_SUMO_rev_2	47	57	PF00179	0.598
TRG_DiLeu_BaEn_2	372	378	PF01217	0.425
TRG_DiLeu_BaEn_4	108	114	PF01217	0.546
TRG_DiLeu_BaLyEn_6	512	517	PF01217	0.425
TRG_DiLeu_BaLyEn_6	538	543	PF01217	0.389
TRG_DiLeu_BaLyEn_6	63	68	PF01217	0.560
TRG_ENDOCYTIC_2	177	180	PF00928	0.402
TRG_ENDOCYTIC_2	282	285	PF00928	0.395
TRG_ENDOCYTIC_2	290	293	PF00928	0.260
TRG_ENDOCYTIC_2	294	297	PF00928	0.245
TRG_ENDOCYTIC_2	360	363	PF00928	0.360
TRG_ENDOCYTIC_2	463	466	PF00928	0.304
TRG_ENDOCYTIC_2	514	517	PF00928	0.304
TRG_ENDOCYTIC_2	656	659	PF00928	0.320
TRG_ER_diArg_1	492	495	PF00400	0.374
TRG_ER_diArg_1	496	498	PF00400	0.323
TRG_ER_diArg_1	64	67	PF00400	0.474
TRG_NES_CRM1_1	328	341	PF08389	0.257
TRG_NES_CRM1_1	662	675	PF08389	0.451
TRG_Pf-PMV_PEXEL_1	541	546	PF00026	0.425

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IC26	Leptomonas seymouri	62%	99%
A0A0S4J452	Bodo saltans	32%	100%
A0A1X0PAG3	Trypanosomatidae	44%	100%
A0A3R7LBT2	Trypanosoma rangeli	42%	100%
A0A3S7X813	Leishmania donovani	94%	100%
A4HAT4	Leishmania braziliensis	80%	83%
A4I9Z4	Leishmania infantum	94%	85%
C9ZMN4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9B508	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q3TIU4	Mus musculus	25%	100%
Q6AXQ5	Rattus norvegicus	25%	100%
Q8W0Z9	Arabidopsis thaliana	24%	100%
V5DGT6	Trypanosoma cruzi	42%	100%