Putative G-actin binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

twinfilin

Species:

Leishmania major

UniProt:

Q4Q2X1_LEIMA

TriTrypDb:

LmjF.34.2290 , LMJLV39_340027600 , LMJSD75_340028500

Length:

331

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005884	actin filament	6	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0097014	ciliary plasm	5	2
GO:0099080	supramolecular complex	2	2
GO:0099081	supramolecular polymer	3	2
GO:0099512	supramolecular fiber	4	2
GO:0099513	polymeric cytoskeletal fiber	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	12
GO:0005856	cytoskeleton	5	10
GO:0043226	organelle	2	10
GO:0043228	non-membrane-bounded organelle	3	10
GO:0043229	intracellular organelle	3	10
GO:0043232	intracellular non-membrane-bounded organelle	4	10

Expansion

Sequence features

Q4Q2X1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2X1

Function

Biological processes
Term	Name	Level	Count
GO:0007015	actin filament organization	5	2
GO:0008064	regulation of actin polymerization or depolymerization	6	12
GO:0008154	actin polymerization or depolymerization	6	2
GO:0009987	cellular process	1	12
GO:0010639	negative regulation of organelle organization	6	12
GO:0016043	cellular component organization	3	12
GO:0022411	cellular component disassembly	4	2
GO:0030042	actin filament depolymerization	7	2
GO:0030832	regulation of actin filament length	5	12
GO:0030833	regulation of actin filament polymerization	7	12
GO:0030834	regulation of actin filament depolymerization	7	2
GO:0030835	negative regulation of actin filament depolymerization	7	2
GO:0030837	negative regulation of actin filament polymerization	7	12
GO:0031333	negative regulation of protein-containing complex assembly	6	12
GO:0032271	regulation of protein polymerization	6	12
GO:0032272	negative regulation of protein polymerization	7	12
GO:0032507	maintenance of protein location in cell	3	2
GO:0032535	regulation of cellular component size	4	12
GO:0032956	regulation of actin cytoskeleton organization	5	12
GO:0032970	regulation of actin filament-based process	4	12
GO:0032984	protein-containing complex disassembly	5	2
GO:0033043	regulation of organelle organization	5	12
GO:0042989	sequestering of actin monomers	4	2
GO:0043242	negative regulation of protein-containing complex disassembly	6	2
GO:0043244	regulation of protein-containing complex disassembly	5	2
GO:0043254	regulation of protein-containing complex assembly	5	12
GO:0043933	protein-containing complex organization	4	2
GO:0044087	regulation of cellular component biogenesis	4	12
GO:0045185	maintenance of protein location	3	2
GO:0048519	negative regulation of biological process	3	12
GO:0048523	negative regulation of cellular process	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0051016	barbed-end actin filament capping	9	2
GO:0051128	regulation of cellular component organization	4	12
GO:0051129	negative regulation of cellular component organization	5	12
GO:0051179	localization	1	2
GO:0051235	maintenance of location	2	2
GO:0051261	protein depolymerization	6	2
GO:0051493	regulation of cytoskeleton organization	6	12
GO:0051494	negative regulation of cytoskeleton organization	7	12
GO:0051651	maintenance of location in cell	2	2
GO:0051693	actin filament capping	8	2
GO:0065007	biological regulation	1	12
GO:0065008	regulation of biological quality	2	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0090066	regulation of anatomical structure size	3	12
GO:0097435	supramolecular fiber organization	4	2
GO:0110053	regulation of actin filament organization	6	12
GO:1901879	regulation of protein depolymerization	6	2
GO:1901880	negative regulation of protein depolymerization	7	2
GO:1902903	regulation of supramolecular fiber organization	5	12
GO:1902904	negative regulation of supramolecular fiber organization	6	12

Molecular functions
Term	Name	Level	Count
GO:0003779	actin binding	4	12
GO:0003785	actin monomer binding	5	2
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0008092	cytoskeletal protein binding	3	12
GO:0044877	protein-containing complex binding	2	2
GO:0051015	actin filament binding	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	307	309	PF00675	0.642
CLV_PCSK_KEX2_1	116	118	PF00082	0.556
CLV_PCSK_PC1ET2_1	116	118	PF00082	0.544
CLV_PCSK_SKI1_1	137	141	PF00082	0.546
CLV_PCSK_SKI1_1	218	222	PF00082	0.319
CLV_PCSK_SKI1_1	227	231	PF00082	0.322
CLV_PCSK_SKI1_1	296	300	PF00082	0.548
CLV_PCSK_SKI1_1	35	39	PF00082	0.497
CLV_PCSK_SKI1_1	60	64	PF00082	0.554
CLV_PCSK_SKI1_1	66	70	PF00082	0.534
DEG_Nend_Nbox_1	1	3	PF02207	0.525
DOC_MAPK_DCC_7	156	166	PF00069	0.607
DOC_PP2B_LxvP_1	40	43	PF13499	0.510
DOC_PP2B_PxIxI_1	254	260	PF00149	0.581
DOC_USP7_MATH_1	301	305	PF00917	0.570
DOC_USP7_MATH_1	44	48	PF00917	0.474
DOC_USP7_UBL2_3	35	39	PF12436	0.508
DOC_WW_Pin1_4	108	113	PF00397	0.553
DOC_WW_Pin1_4	232	237	PF00397	0.572
DOC_WW_Pin1_4	251	256	PF00397	0.374
LIG_14-3-3_CanoR_1	120	125	PF00244	0.492
LIG_14-3-3_CanoR_1	308	314	PF00244	0.664
LIG_14-3-3_CanoR_1	97	107	PF00244	0.501
LIG_BIR_III_2	223	227	PF00653	0.525
LIG_DCNL_PONY_1	1	4	PF03556	0.591
LIG_FHA_1	116	122	PF00498	0.508
LIG_FHA_1	191	197	PF00498	0.490
LIG_FHA_1	22	28	PF00498	0.491
LIG_FHA_1	286	292	PF00498	0.575
LIG_FHA_1	57	63	PF00498	0.453
LIG_FHA_1	79	85	PF00498	0.530
LIG_FHA_2	143	149	PF00498	0.449
LIG_FHA_2	173	179	PF00498	0.578
LIG_FHA_2	220	226	PF00498	0.581
LIG_FHA_2	288	294	PF00498	0.525
LIG_LIR_Gen_1	225	236	PF02991	0.549
LIG_LIR_Gen_1	69	79	PF02991	0.375
LIG_LIR_Gen_1	80	88	PF02991	0.354
LIG_LIR_Nem_3	225	231	PF02991	0.549
LIG_LIR_Nem_3	297	302	PF02991	0.586
LIG_LIR_Nem_3	69	75	PF02991	0.362
LIG_LIR_Nem_3	80	86	PF02991	0.355
LIG_SH2_PTP2	247	250	PF00017	0.501
LIG_SH2_PTP2	72	75	PF00017	0.502
LIG_SH2_PTP2	83	86	PF00017	0.516
LIG_SH2_STAP1	261	265	PF00017	0.562
LIG_SH2_STAP1	54	58	PF00017	0.434
LIG_SH2_STAT5	247	250	PF00017	0.487
LIG_SH2_STAT5	72	75	PF00017	0.497
LIG_SH2_STAT5	83	86	PF00017	0.403
LIG_SH2_STAT5	87	90	PF00017	0.351
LIG_SH3_3	161	167	PF00018	0.515
LIG_SH3_3	24	30	PF00018	0.542
LIG_SH3_3	245	251	PF00018	0.544
LIG_SUMO_SIM_par_1	29	34	PF11976	0.426
LIG_TRAF2_1	15	18	PF00917	0.462
LIG_TYR_ITSM	79	86	PF00017	0.452
LIG_WRC_WIRS_1	191	196	PF05994	0.581
MOD_CDK_SPK_2	232	237	PF00069	0.593
MOD_CDK_SPK_2	251	256	PF00069	0.407
MOD_CDK_SPxxK_3	251	258	PF00069	0.562
MOD_CK1_1	122	128	PF00069	0.496
MOD_CK1_1	219	225	PF00069	0.559
MOD_CK1_1	235	241	PF00069	0.471
MOD_CK1_1	89	95	PF00069	0.515
MOD_CK2_1	120	126	PF00069	0.474
MOD_CK2_1	127	133	PF00069	0.441
MOD_CK2_1	142	148	PF00069	0.383
MOD_CK2_1	294	300	PF00069	0.510
MOD_GlcNHglycan	17	21	PF01048	0.514
MOD_GlcNHglycan	263	266	PF01048	0.342
MOD_GlcNHglycan	296	299	PF01048	0.488
MOD_GlcNHglycan	303	306	PF01048	0.598
MOD_GlcNHglycan	92	95	PF01048	0.446
MOD_GSK3_1	115	122	PF00069	0.453
MOD_GSK3_1	313	320	PF00069	0.705
MOD_GSK3_1	74	81	PF00069	0.481
MOD_GSK3_1	86	93	PF00069	0.515
MOD_GSK3_1	98	105	PF00069	0.546
MOD_LATS_1	311	317	PF00433	0.518
MOD_N-GLC_1	56	61	PF02516	0.441
MOD_NEK2_1	16	21	PF00069	0.486
MOD_NEK2_1	203	208	PF00069	0.581
MOD_NEK2_1	56	61	PF00069	0.525
MOD_NEK2_1	98	103	PF00069	0.444
MOD_PIKK_1	179	185	PF00454	0.509
MOD_PIKK_1	235	241	PF00454	0.498
MOD_PIKK_1	78	84	PF00454	0.509
MOD_PK_1	120	126	PF00069	0.327
MOD_PKA_2	119	125	PF00069	0.486
MOD_PKA_2	307	313	PF00069	0.657
MOD_Plk_1	203	209	PF00069	0.502
MOD_Plk_4	127	133	PF00069	0.411
MOD_Plk_4	216	222	PF00069	0.526
MOD_Plk_4	325	331	PF00069	0.648
MOD_ProDKin_1	108	114	PF00069	0.556
MOD_ProDKin_1	232	238	PF00069	0.572
MOD_ProDKin_1	251	257	PF00069	0.374
MOD_SUMO_for_1	194	197	PF00179	0.539
MOD_SUMO_rev_2	130	139	PF00179	0.553
TRG_DiLeu_BaEn_1	36	41	PF01217	0.504
TRG_ENDOCYTIC_2	72	75	PF00928	0.457
TRG_ENDOCYTIC_2	83	86	PF00928	0.421

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8Y3	Leptomonas seymouri	63%	100%
A0A0S4JJ86	Bodo saltans	26%	100%
A0A1X0P9W3	Trypanosomatidae	33%	97%
A0A3R7M8Y8	Trypanosoma rangeli	30%	100%
A0A3S7X7X8	Leishmania donovani	92%	100%
A4HAU1	Leishmania braziliensis	74%	100%
A4IA09	Leishmania infantum	92%	100%
C9ZMM6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9B515	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
V5BPY6	Trypanosoma cruzi	30%	100%